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Citations for GE Life Sciences :
701 - 750 of 5414 citations for Human IgM Anti Flavivirus Envelope Protein Antibody 4G2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Culture supernatants were clarified followed by protein A affinity chromatography with MabSelect SuRe LX protein A resin (GE Healthcare) using the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then washed twice with PBS and incubated for 1 h with anti-mouse IgG horseradish peroxidase (HRP)-conjugated sheep antibody (GE Healthcare) diluted 1:4,000 in antibody dilution buffer ...
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bioRxiv - Microbiology 2020Quote: ... ELISA plates were washed 3 times with PBST and incubated in 50 μL per well of sheep anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (GE Healthcare) or goat anti-hamster IgG-HRP conjugated antibody (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... ELISA plates were washed 3 times with PBST and 50 µL of sheep anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (GE Healthcare) was added at a dilution of 1:3,000 in blocking solution ...
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bioRxiv - Biochemistry 2019Quote: ... the strips were washed with TBS and incubated with anti-rabbit IgG HRP-conjugated antibodies (RRID: AB_772206, GE Healthcare, Pittsburgh, PA) at a 1:20,000 dilution in TBS + 3% BSA ...
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bioRxiv - Microbiology 2019Quote: ... plaque assays were washed with PBS three times and then incubated for 1 h in anti-mouse IgG horseradish peroxidase (HRP)-conjugated sheep antibody (GE Healthcare) diluted 1:4,000 in antibody dilution buffer ...
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bioRxiv - Neuroscience 2019Quote: ... mouse monoclonal anti-tubulin antibody (1:1000, DSHB, University of Iowa, Iowa City, IA, USA) and secondary anti-mouse IgG-HRP (1:2000, GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... and detection of enhanced chemilluminescence from HRP-coupled anti-rabbit secondary antibodies followed by detection with an ImageQuant LAS4000 mini imaging system and analysis with ImageQuant software (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... and then detected with an anti-rabbit horseradish peroxidase-linked secondary antibody using an ImageQuant LAS 4000 mini digital imaging system (GE Healthcare). Detection of DnaA ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated for 1 h with 5 μL of anti-MYC antibody (Gentex) followed by 1 h incubation with an additional 50 μL of GammaBind plus Sepharose beads (GE Healthcare) to capture protein complexes ...
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bioRxiv - Microbiology 2022Quote: ... Aldolase was detected using the above protocol with 1:10,000 mouse anti-aldolase antiserum (a kind gift from David Sullivan) and 1:2,500 sheep anti-mouse HRP-linked secondary antibodies (GE healthcare, NA931).
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bioRxiv - Immunology 2022Quote: ... ELISA plates were afterwards washed 3 times with PBST and 50 μL of anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (Cytiva, GE Healthcare) was added at a dilution of 1:3,000 in blocking solution ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were then incubated for 1 h with a secondary antibody (goat anti-rabbit horseradish peroxidase-linked IgG [NA934V GE Healthcare] or sheep anti-mouse horseradish peroxidase-linked IgG [NA931V GE Healthcare] ...
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bioRxiv - Microbiology 2020Quote: ... Secondary antibody was horseradish peroxidase (HRP)-conjugated anti-mouse IgG or IgA and a chemiluminescence HRP was used as substrate (GE Healthcare). Apparent molecular weight of bands was determined using a standard curve generated from molecular weight markers (r2=0.9916 ...
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bioRxiv - Molecular Biology 2022Quote: Purified NiV F was immobilized to a single flow cell of either (i) a CM5 sensor functionalized with an anti-StreptagII antibody or a NiNTA sensor in a Biacore X100 (GE Healthcare). Binding kinetics for Fabs 4H3 and 1H8 were measured using a CM5 sensor ...
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bioRxiv - Physiology 2022Quote: ... membranes were washed three times with 1X TBST and incubated with secondary antibodies in 5% milk in TBST for one hour at room temperature with the following concentration of secondary antibodies: donkey anti-rabbit IgG (1:5000, NA934, GE Healthcare) and goat anti-mouse IgG (1:5000 ...
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bioRxiv - Cell Biology 2019Quote: ... G2 and G1 phases based on the signal intensity of anti-CENPF antibody staining with software (IN Cell Investigator, GE Healthcare).
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bioRxiv - Neuroscience 2019Quote: ... The blots were exposed to a peroxidase-conjugated anti-mouse secondary antibody and ECL Advance chemiluminescent substrate (GE Healthcare, Piscataway, NJ). The resulting chemilluminescent signal was acquired with a Molecular Imager Chemidoc EQ system (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... between 400 to 600 RU of biotinylated anti-IFX monoclonal antibodies were immobilised on a streptavidin-coated sensor chip prepared using the Biotin CAPture kit (GE Healthcare); a biotinylated mouse monoclonal antibody (OX68 ...
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bioRxiv - Cancer Biology 2021Quote: ... or HRP-conjugated anti-mouse (CST, 7076; 1:1000) IgG secondary antibodies and visualized using ECL Prime Western Blotting Detection Reagent (GE Healthcare) and ImageQuant LAS 4000 Mini (FUJIFILM) ...
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bioRxiv - Microbiology 2021Quote: ... were used for immunoblotting along with sheep anti-mouse-HRP NXA931V secondary antibody (GE Healthcare, 1:5,000 or 1:10,000, respectively). All yeast growth and Western blot data shown are representative of at least two biological replicates.
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated for 1 h at room temperature with secondary antibody (anti-mouse IgG, 1:10,000 in 3% BSA/TBS-T; GE Healthcare, #RPN4201) linked to horseradish peroxidase ...
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bioRxiv - Cell Biology 2023Quote: ... The following secondary antibodies were used for immunoblotting: HRP-conjugated donkey anti-rabbit IgG (1:2000) (GE Healthcare, catalogue number NA934) and HRP-conjugated donkey anti-mouse IgG (1:5000 ...
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bioRxiv - Biochemistry 2023Quote: ... Bound proteins were eluted by boiling in SDS–PAGE sample loading buffer and analyzed by western blot using an anti-GST antibody (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... membranes were washed three times with TBS-Tween 1 % and incubated for 1 h at room temperature with a secondary antibody anti-Mouse conjugated to horseradish peroxidase (GE Healthcare, NA9310 ...
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bioRxiv - Biochemistry 2023Quote: ... Anti-rabbit horseradish peroxidase-linked IgG and anti-mouse IgG, horseradish peroxidase linked antibodies (NA944V and NA931, respectively) were from GE Healthcare. Polystyrene round-bottom tubes (5 mL ...
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bioRxiv - Immunology 2023Quote: ... An Fc capture chip was produced by amine coupling of anti-mouse IgG antibody to a CM5 chip using a commercial Fc Capture Kit (GE Healthcare). First ...
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bioRxiv - Cell Biology 2023Quote: ... The following secondary antibodies were used in immunoblotting: HRP-conjugated donkey anti-rabbit IgG (1:2000) (GE Healthcare, catalog number AB_772206), HRP-conjugated goat anti-mouse IgG (1:5000 ...
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bioRxiv - Molecular Biology 2021Quote: ... column for Notch1fe (human version) or a Superdex 10/300 increase (GE Healthcare) column for Jagged1fe,HA and Notch1NRR ...
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bioRxiv - Microbiology 2020Quote: Neutrophils isolated from fresh human blood using a Ficoll-Paque PLUS (GE Healthcare) gradient were resuspended in medium at a concentration of 5 × 106 cells/mL ...
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bioRxiv - Cell Biology 2019Quote: ... human adult blood was collected and immune cells purified by Ficoll (GE healthcare). Mice were injected intravenously with 1×107 freshly isolated PBMCs or NK-depleted PBMCs obtained from the negative fraction of a positive selection (CD56+ ...
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bioRxiv - Genomics 2024Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Paque (GE Healthcare) density centrifugation from buffy coats (American Red Cross ...
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bioRxiv - Microbiology 2020Quote: ... proteins were immobilized to a protein A sensorchip a level of ~500 response units (RUs) using Biacore 8000 (GE Healthcare) and a running buffer of composed of 20mM PB7.4 with 300 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... Protein was purified via its C-terminal green fluorescence protein (GFP) tag using GFP nanobody coupled Sepharose Beads (GE Healthcare) and eluted by removing the GFP tag with the PreScission Protease ...
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bioRxiv - Biophysics 2019Quote: The proteins and protein complexes were analyzed by analytical gel filtration using a Superdex 200 5/150 column (GE Healthcare) and the 1260 Infinity Bio-inert high-performance liquid chromatography system (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... The protein absorbance at 280 nm was monitored to detect protein elution on an AKTA system (GE Healthcare Life Sciences). The eluted fractions were collected for SDS-PAGE analysis.
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bioRxiv - Biochemistry 2022Quote: ... Cleaved protein was further purified by fast protein liquid chromatography (FPLC) using a Source-S cation-exchange column (GE Healthcare) (low salt buffer with 50 mM potassium phosphate at pH 7.0 ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins were further purified by gel filtration on a fast protein liquid chromatography (FPLC) system (GE Healthcare Life Sciences). The principle peaks were collected ...
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bioRxiv - Zoology 2021Quote: ... Visualization of identified total protein and phosphorylated protein bands was completed using an Amershan ECL Plus detection kit (GE Healthcare) as per manufacturer’s instructions (Figure A1 ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was then loaded onto the protein A column and the target protein was eluted with 0.1 M acetic acid on ÄKTA pure (GE Healthcare). The collected protein was added to 1 mM Edetate disodium (EDTA) ...
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bioRxiv - Developmental Biology 2021Quote: ... The remaining supernatant was added to 50 µl G-Protein Sepharose beads (Protein G-Sepharose 4 Fast Flow, GE Healthcare) (pre-washed with the lysis buffer ...
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bioRxiv - Bioengineering 2021Quote: ... Protein G-coupled sepharose (#17–0618–01) and Protein A-coupled sepharose (#17–5138–01) beads were from GE Healthcare and the protease inhibitor cocktail was from Sigma.
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bioRxiv - Biophysics 2023Quote: ... Protein was purified via its C-terminal green fluorescence protein (GFP) tag using GFP nanobody-coupled Sepharose beads (GE Healthcare) and eluted by removing the GFP tag with PreScission Protease ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies were detected with peroxidase-conjugated secondary antibodies (GE Healthcare).
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bioRxiv - Biophysics 2021Quote: ... Protein-transferred PVDF membranes (GE Healthcare Life Science) were blocked with 5% skim milk in PBS-T ...
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bioRxiv - Biophysics 2021Quote: ... proteins were visualized using ECL prime (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: M-PER mammalian protein extraction reagent (GE Healthcare) was used to extract protein from mOSE cells and run on NuPAGE 4-12% Bis-Tris gradient gels (Life Technologies).
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bioRxiv - Molecular Biology 2020Quote: ... Protein transfer onto nitrocellulose membranes (GE Life Sciences) was carried out for 90 min at 25 W in a Mini Trans-Blot Electrophoretic Transfer Cell (BioRad) ...
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bioRxiv - Biochemistry 2019Quote: ... 45 μl Protein G Sepharose beads (GE Healthcare) were added ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein G Sepharose beads CL-4B (GE Healthcare) were pre-equilibrated with lysis buffer ...