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Citations for GE Life Sciences :
501 - 550 of 5411 citations for Human IgG1 Anti Flavivirus Envelope Protein Antibody 4G2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... membranes were probed with an ECL anti-rabbit IgG secondary antibody (1:10,000) from GE Healthcare Life Science and imaged using Chemidoc Molecular Imaging System (BioRad) ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibody used in Western blotting was HRP sheep anti-mouse IgG (GE Life Sciences NA931V).
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bioRxiv - Biophysics 2024Quote: ... the membrane was incubated with Anti-mouse HPR-conjugated secondary antibody (1:5000 dilution, GE Healthcare) at RT for 2 hours and visualized with enhanced chemiluminescence using ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Molecular Biology 2023Quote: ... 1,000 μg of proteins were prepared and pre-cleared with 20 μl of protein A-Sepharose and protein G-Sepharose beads mixture (GE Healthcare). 2 μl of (HA ...
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bioRxiv - Microbiology 2021Quote: ... Anti-Fh15 polyclonal IgG was purified by affinity chromatography using 5/5/HiTrap Protein-A HP (GE Healthcare, Piscataway, NJ). The resulting IgG was conjugated with horseradish peroxidase (HRP ...
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bioRxiv - Cell Biology 2021Quote: ... They were then incubated with a horseradish peroxidase-conjugated secondary antibody and proteins were detected using enhanced chemiluminescence (GE Healthcare Life Sciences, RPN2209). Densitometry analysis of bands was performed using Image J software.
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bioRxiv - Cancer Biology 2022Quote: ... cell lysates were immunoprecipitated with the indicated antibodies for 3 h at 4 °C and then incubated with protein G (GE Healthcare Life Sciences) for 2 hrs at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Antibodies were affinity-purified from filtered cultured supernatant on a High-Trap protein G column (GE Healthcare, Chicago, USA, Cat.Nr 17-0404-01).
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bioRxiv - Molecular Biology 2021Quote: ... NET2 buffer was added to a final volume of 1 mL with 20 µL of Glutathione Sepharose 4B or SF3b155a-antibody conjugated with Protein G Sepharose (GE Healthcare Life Sciences) and incubated at 4°C for 3 h ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were incubated with antibodies for one hour at 4°C in an overhead tumbler before immune complexes were retrieved by protein G (GE Healthcare or abcam) or A Sepharose (GE Healthcare) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The secondary antibody was incubated for 2 hours (Anti-rabbit, GE Healthcare, 1:10000 or Anti-mice, GE Healthcare, 1:10000, Chicago, Illinois, USA). The bands were detected using chemiluminescence methods (ECLPrime® ...
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bioRxiv - Cell Biology 2022Quote: ... and membranes were incubated with secondary antibodies conjugated to horseradish peroxidase (anti-mouse or anti-rabbit; GE Healthcare 17097199 and 16951542, 1:5000 dilution) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated for 1 h at room temperature with 1:10,000 dilutions of anti-rabbit or anti-mouse secondary antibodies (GE Healthcare Life Sciences, Pittsburgh, PA), washed 3X with TBS-T ...
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bioRxiv - Molecular Biology 2022Quote: ... Human cDNA of JAZF1 was purchased from GE Healthcare. Open reading frame (ORF ...
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bioRxiv - Immunology 2021Quote: Human PBMCs were separated on Ficoll-Paque (GE Healthcare) by density centrifugation of heparinized blood from healthy donors (“Etablissement Français du Sang” EFS ...
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bioRxiv - Developmental Biology 2019Quote: ... 1ml supernatant (2mg protein) was incubated with 30μl Protein G Sepharose (GE Healthcare) for two hours on a rotator at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were pre-cleared using 40 μl of Protein G Sepharose (GE Healthcare), for 1 hour at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Soluble Ig-fused proteins were purified using protein A-Sepharose beads (GE Healthcare). Bound proteins were eluted with 0.1 M glycine (pH 2.5 ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were further incubated with protein A/G-sepharose beads (GE Healthcare) for 2 hours ...
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bioRxiv - Biochemistry 2022Quote: ... Fc-fused ACE2 proteins were purified on HiTrap Protein A column (GE Healthcare) and eluted with 50 mM Tris pH 7.2 ...
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bioRxiv - Immunology 2022Quote: ... fusion protein was purified from cell supernatant using protein A column (GE healthcare). After digestion with TEV protease ...
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bioRxiv - Cancer Biology 2022Quote: Human peripheral blood mononuclear cells (PBMC) were purified from human blood buffy coat using Ficoll Paque Plus (GE Healthcare, cat. 17-1440-02) reagent and CD14+ microbeads (MACS Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were washed with PBST and incubated with alkaline phosphatase-conjugated secondary antibodies (anti-rabbit IgG and anti-mouse IgG, GE healthcare life sciences, 1:10000) for 1 hr at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... the membranes were incubated for 90 minutes at room temperature with secondary antibodies (donkey anti-rabbit HRP-IgG, GE Healthacare, cat#NA934V, 1:5000 or goat anti-mouse HRP-IgG, GE Healthcare, cat#NA931V, 1:2000) diluted in blocking solution and washed as before ...
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bioRxiv - Cancer Biology 2022Quote: ... 1:100 Dako #M7248) (overnight, 4 °C) and biotinylated secondary antibodies (HRP anti-mouse and HRP anti-rabbit, 1:200, GE Healthcare (2h at room temperature). Target proteins were detected with the ABC Kit and the DAB kit (Vector Laboratories).
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bioRxiv - Molecular Biology 2021Quote: ... 300 µg of the prepared lysate was incubated with the appropriate antibody (3 µg) and pulled down using protein A Sepharose 6MB (GE healthcare # 17-0469-01). All primary antibodies used in this study are listed in table T3 ...
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bioRxiv - Cell Biology 2019Quote: ... using a HiTrap NHS-Activated HP affinity column coupled with aIL-4 monoclonal antibodies and an ÄKTA Fast Protein Liquid Chromatography (FPLC) instrument (GE Healthcare, Piscataway, NJ, USA). Immunizations ...
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bioRxiv - Cell Biology 2021Quote: ... For co-immunoprecipitation of endogenous SQSTM1/p62 the cell lysates were incubated for 2-4 h with specific antibody followed by 1-2 h with protein-G coupled Sepharose beads (GE Healthcare, 17-0885-01). To resolve protein complexes for denaturing immunoprecipitation ...
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bioRxiv - Biophysics 2021Quote: ... Total protein was collected and incubated with anti-p75NTR (Alomone, ANT-007, 1ug) overnight at 4 °C and then incubated with Sepharose protein-G beads (GE Healthcare). Samples were then prepared for immunoblotting as described below.
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bioRxiv - Molecular Biology 2019Quote: ... The remainder of the lysates were incubated with polyclonal rabbit anti-GFP (EU2; Eusera) for 1 hour followed by Protein A-Sepharose beads (GE Healthcare) for another hour at 4° C ...
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bioRxiv - Cell Biology 2020Quote: ... and peroxidase-conjugated donkey anti-rabbit IgG antibody diluted in 1:2000–1:4000 (NA934, GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... peroxidase-conjugated sheep anti-mouse IgG antibody diluted in 1:4000 (NA931, GE Healthcare, Little Chalfont, UK); and peroxidase-conjugated donkey anti-rabbit IgG antibody diluted in 1:2000–1:4000 (NA934 ...
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bioRxiv - Molecular Biology 2021Quote: ... Horseradish peroxidase (HRP)-linked secondary antibodies included anti-mouse IgG (sheep polyclonal, NA931: GE Healthcare, Buckinghamshire, UK), anti-rabbit IgG (sheep polyclonal ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were incubated with anti-mouse HRP-conjugated secondary antibody from GE Healthcare (Cat. NA9311ML, Chicago, USA). The bands were analyzed by an Immobilon Western Chemiluminescent HRP Substrate (Millipore ...
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bioRxiv - Cell Biology 2020Quote: Secondary antibodies and stains used were: IgG peroxidase-conjugated anti-mouse IgG from donkey (GE Healthcare, NA931), anti-rabbit IgG from donkey (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... anti-ABCA4 antibody (CL2) to CNBr-activated Sepharose 4B beads (GE Healthcare Bio-Sciences, Chicago, IL, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... the membranes were probed with 1:2,500 goat anti-rat HRP-linked secondary antibodies (GE healthcare, NA935) in 5% milk powder/PBS solution for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... samples were incubated with anti-LptD antibodies and nProtein A Sepharose™ 4 Fast Flow (GE healthcare) at 4°C over-night with slow rotation ...
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bioRxiv - Microbiology 2020Quote: ... and revealed with horseradish peroxidase (HRP)-conjugated anti-rabbit IgG secondary antibody (1:3,000; GE Healthcare, USA). Blots were developed using a Pierce ECL chemiluminescence substrate (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were incubated with anti-Rabbit HRP-conjugated secondary antibody (1:5000 of NA934, GE Healthcare) for 1 hour at room temperature and subsequently washed with PBST thrice ...
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bioRxiv - Physiology 2021Quote: ... or anti-goat IgG antibodies and Amersham ECLTM Prime Western Blotting Detection Reagent (GE Healthcare, Buckinghamshire, UK). Band quantification was carried out using an Image Station 2000R (Kodak ...
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bioRxiv - Microbiology 2021Quote: ... The membrane was then incubated with an HRP-conjugated secondary antibody (ECL anti-rabbit IgG, GE Healthcare) and PstS bands were visualized with the ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Neuroscience 2021Quote: Secondary antibodies for western blot analysis were ECL Plex goat anti-rabbit IgG-Cy5 (PA45012, GE Healthcare), ECL Plex goat anti-mouse IgG-Cy3 (PA43009 ...
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bioRxiv - Neuroscience 2022Quote: ... the membranes incubated with an anti-mouse HRP-linked secondary antibody (1:5000, GE Healthcare, NXA931, RRID:AB_772209). Either Pierce ECL Western Blotting Substrate (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were detected using either HRP-linked donkey anti–rabbit IgG (GE Healthcare, #NA934V; 1:10,000) or HRP-linked sheep anti– mouse IgG (GE Healthcare ...
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bioRxiv - Neuroscience 2023Quote: HRP-conjugated secondary antibodies raised against the appropriate species were used: anti- mouse IgG (GE Healthcare, #NA931), anti-rabbit IgG (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: ... Purified GST-Slp1(456-488aa) was detected with either goat anti-GST HRP-conjugated antibodies (GE Healthcare) or rabbit polyclonal anti-pThr480 antibodies.
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bioRxiv - Cell Biology 2022Quote: The real time protein-protein interaction of protease and PDZ domain proteins of PfHtrA2was analysed by SPR using Biacore T200 instrument (GE Healthcare, Uppsala, Sweden) as described previously [7] ...
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bioRxiv - Biochemistry 2019Quote: His6-tagged proteins were purified by binding proteins to Ni-NTA columns (GE Healthcare) in buffer D (50 mM Tris pH 8.0 ...
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bioRxiv - Developmental Biology 2021Quote: ... Extracted proteins were immunoprecipitated with Protein A Sepharose CL-4B beads (GE Healthcare, USA) and desired primary antibodies ...