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Citations for GE Life Sciences :
251 - 300 of 972 citations for Human Galectin 3 LGALS3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... at 55 cm Hg for 1.5 hrs with RNA transfer buffer (3 M NaCl, 0.01 N NaOH) using a VacuGene XL (GE Healthcare). The membrane was rinsed with 2x SSC for 10 min ...
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bioRxiv - Cell Biology 2024Quote: Col1a1-H2B-GFP;Rosa26-M2-rtTA;Slc23a2FL/FL;Mx1-Cre mice and Col1a1-H2B-GFP;Rosa26-M2-rtTA;Slc23a2FL/FL littermate controls were given 3 intraperitoneal injections of 40µg of poly I:C (GE Healthcare) dissolved in PBS over 5 days to induce Cre expression ...
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bioRxiv - Cell Biology 2024Quote: ... Mx1-Cre mice were given 3 intraperitoneal injections over 5 days of 40µg of polyinosinic-polycytidilic acid (poly I:C) (GE Healthcare) dissolved in PBS ...
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bioRxiv - Evolutionary Biology 2021Quote: ... or with a GFX PCR DNA kit and Gel Band Purification kit (GE Healthcare, USA) following manufacturer guidelines ...
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bioRxiv - Immunology 2022Quote: ... Human monocytes were purified from buffy coats by two gradient centrifugations: PBMCs were initially isolated with Ficoll-Paque (GE Healthcare, Pittsburgh, PA) and subsequently peripheral monocytes were isolated with Percoll (GE Healthcare ...
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bioRxiv - Genetics 2021Quote: SARS-CoV-2 receptor binding domain binding to human extracellular ACE2 were analysed on a Biacore T200 instrument (GE Healthcare Life Sciences) at 37°C and a flow rate of 30 μl/min ...
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bioRxiv - Immunology 2023Quote: FH was isolated from human plasma using an affinity column made up of a monoclonal antibody against human FH (MRCOX23; MRC Immunochemistry Unit, University of Oxford) coupled to CNBr-activated Sepharose (GE Healthcare, UK) as described previously (21) ...
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bioRxiv - Immunology 2022Quote: ... Plates were subsequently washed 4 times with washing buffer and incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, NA933) at a 1:5000 dilution in PBS + 0.05% Tween-20 ...
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bioRxiv - Immunology 2022Quote: ... Plates were subsequently washed 4 times with washing buffer and incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, NA933) at a 1:5000 dilution in PBS + 0.05% Tween-20 ...
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bioRxiv - Cancer Biology 2023Quote: Binding of mAbs to human FcRn was performed by measuring surface plasmon resonance (SPR) using a Biacore T200 instrument (GE Healthcare, UK), as described previously (42).
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bioRxiv - Immunology 2024Quote: Human PBMCs were isolated from heparinised blood and tissue derived mononuclear cells were isolated from mechanically dissociated and filtered human tissue using Ficoll density gradient centrifugation (Ficoll PaquePlus; GE Healthcare, Amersham) or 33% Percoll (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: Human HeLa (ATCC® CCL-2™) were cultured in DMEM (PanEco) supplemented with 10% fetal bovine serum (FBS; HyClone/GE Healthcare) and penicillin/streptomycin ...
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bioRxiv - Developmental Biology 2019Quote: ... peroxidase-linked species-specific F(ab’)2 fragment (from donkey) (1:10,000 in 3% skim milk/TBST, NA9340, GE Healthcare) Mouse TrueBlot ULTRA ...
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bioRxiv - Developmental Biology 2019Quote: ... peroxidase-linked species-specific F(ab’)2 fragment (from sheep) (1:10,000 in 3% skim milk/TBST, NA9310, GE Healthcare) ECL anti-rabbit IgG ...
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bioRxiv - Developmental Biology 2019Quote: ... the membrane was incubated with an appropriate secondary antibody (anti-rabbit or anti-mouse IgG HRP-conjugated Ab (1:10,000 in 3% skim milk in TBS, GE Healthcare), mouse TrueBlot ULTRA ...
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bioRxiv - Biochemistry 2019Quote: ... to 3 ml and the protein further purified by size exclusion chromatography (Hiload 16/600 Superdex 200 pg, GE healthcare) equilibrated with SEC buffer (20 mM HEPES ...
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bioRxiv - Microbiology 2019Quote: ... Wet gels were exposed to a phosphor screen for 0.5 h to 3 h and then scanned using a Typhoon FLA 7000 imaging system (GE Healthcare), after which gels were stained with Coomassie Brilliant Blue.
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bioRxiv - Cancer Biology 2021Quote: ... Cells were transfected with 1-3 nM non-targeting siRNA as a control (D-001810-10-05, GE Healthcare Dharmacon) or with siRNAs targeting PDK2 and PYCR1 (Dharmacon ...
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bioRxiv - Bioengineering 2020Quote: ... and 35 ml of this suspension was carefully added to 15 ml Ficol-Paque PLUS (1.077 g cm-3, GE Healthcare) and centrifuged at RT ...
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bioRxiv - Neuroscience 2020Quote: ... washed three times with 0.1 M cacodylate and then incubated for 3-h at RT in streptavidin HRP conjugate (1:200; GE Healthcare). Sections were visualized after 1-min and 30-sec DAB (Sigma-Aldrich)/glucose oxidase reaction ...
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bioRxiv - Plant Biology 2021Quote: ... and the peptides used for SPR was dissolved in HBS-EP+ (10 mM HEPES, 150 mM NaCl, 3 mM EDTA and 0.05% [v/v] surfactant P20 (GE Healthcare)) ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed 3 times for 5 min each with 1X TBST buffer and developed by chemiluminescence (GE healthcare, RPN2106). The Odyssey FC imaging system (LI-COR ...
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bioRxiv - Immunology 2020Quote: ... IgGs were purified from ∼1-3 ml of serum using equal volume of settled Protein A Sepharose resin (GE Healthcare). Samples were eluted off the resin with 0.1 M glycine pH 2.5 and immediately neutralized with 1 M Tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2020Quote: ... The suspension was centrifuged for 10 minutes at 25,000g and the lysate supernatant was incubated for 3 hours with 1 mL of glutathione sepharose beads (GE Healthcare) pre-washed in lysis buffer ...
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bioRxiv - Systems Biology 2019Quote: ... Samples were separated into 24 fractions using the 24 cm IPG strips pH 3-10 NL (GE HealthCare, Chicago, IL). To achieve the required low salt concentration 2.5 mg wheat germ extract was diluted with water and centrifuged 10 min. ...
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bioRxiv - Biochemistry 2019Quote: ... Wet gels were exposed to phosphor screen (0.5–3 hrs) before being scanned using a Typhoon FLA 7000 imaging system (GE Healthcare). Background subtracted autoradiograph band intensities were determined with ImageStudio (Li-COR ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins into rehydration buffer were incubated with an 18 cm immobilized pH 3–11 nonlinear gradient dry strip (GE Healthcare) for 16 hours at 20°C ...
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bioRxiv - Biophysics 2020Quote: ... A volume of 50 μl of each sample at 3 mg·ml−1 was loaded onto a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in 25 mM Hepes pH 7.8 ...
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bioRxiv - Immunology 2020Quote: ... Then cells were washed 3 times with PBS and stained with 75 μM CypHer5E NHS ester (GE Healthcare, PA #15401) for 1 hour at 25°C[13] ...
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bioRxiv - Biochemistry 2021Quote: ... anti-EGFR antibody (#MI-12-1, MBL), anti-GFP antibody (#M-048-3, MBL) and Protein A-Sepharose (GE Healthcare) were used for immunoprecipitation ...
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bioRxiv - Immunology 2020Quote: ... with a 3 kDa membrane cutoff and loaded onto a HiLoad 16/600 Superdex 75 pg (GE Healthcare Life Sciences) size exclusion chromatography column previously equilibrated with 7M urea ...
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bioRxiv - Cell Biology 2022Quote: ... 3 mM b-mercaptoethanol (BME) and then loaded onto a HiTrap SP HP 5 ml column (GE healthcare, Piscataway, NJ). Elution from the SP column was performed with a 70 ml linear gradient from 0–500 mM NaCl in SP buffer A ...
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bioRxiv - Physiology 2022Quote: ... Membranes were then washed 3 more times and the signal was detected by chemiluminescence using ECL Prime reagent (GE Healthcare) on an Amersham Imager 600 detection system (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... and washed 10 minutes 3 times in PBST before being incubated with horseradish peroxidase-conjugated anti-rabbit IgG (GE Healthcare) diluted 1:2000 in the blocking buffer for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... or with horseradish peroxidase (HRP)-conjugated secondary antibodies followed by a 3-min incubation with a chemiluminescent substrate (GE Healthcare) for ChemiDoc imaging ...
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bioRxiv - Bioengineering 2023Quote: ... All images were acquired in a 3 Tesla MRI with 32 channel receiver system (GE Healthcare, Chicago, Illinois 60661, USA) and made anonymous in compliance with HIPAA standards and rules ...
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bioRxiv - Cell Biology 2022Quote: ... 6 µL of the reaction were diluted to 100 µL in 2x SSC (3 M NaCl, 0.3 M sodium citrate) and slot-blotted on a Hybond XL nylon membrane (GE Healthcare). For detection of ChIP samples ...
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bioRxiv - Microbiology 2023Quote: ... the buffer was exchanged with 1% acetic acid at pH 3 and 0.02% sodium azide by using a HiPrep 26/10 desalting column (GE Healthcare). This ensured that the proteins were maintained in their monomeric form ...
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bioRxiv - Neuroscience 2023Quote: The experiments were conducted using a 3-Tesla scanner equipped with an 8-ch phased array coil (GE Healthcare, Signa). Functional images were acquired using a T2*-weighted gradient-echo echo-planar imaging sequence ...
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bioRxiv - Cell Biology 2020Quote: ... GST kit (GE Healthcare, Chicago, IL) was used for purifying GST-Rheb following manufactures instructions and eluted with reduced glutathione ...
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bioRxiv - Cell Biology 2019Quote: ... ECL chemiluminescent kit (RPN2132, GE Healthcare) was used for detection according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... A His-capture kit (GE Healthcare) was used to immobilize anti-His antibodies on CM5 chips (as recommended by the manufacturer) ...
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bioRxiv - Cancer Biology 2019Quote: ... The His SpinTrap Kit (GE Healthcare) was used according to manufacturer’s recommendations to purify the recombinant CELF1 proteins ...
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bioRxiv - Cell Biology 2019Quote: ... The ECL Prime Kit (GE Healthcare) was used for HRP detection.
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bioRxiv - Microbiology 2019Quote: Human Custom ON-TARGET plus siKSRP or mimic miRs were resuspended in siRNA dilution buffer (Dharmacon, GE Healthcare, Chalfont-St-Giles, United Kingdom) to a 20 µM stock solution ...
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bioRxiv - Immunology 2021Quote: ... Recombinant CEACAM6 protein consisting of the CEACAM6 extracellular domain fused to the constant region of human IgG were produced in HEK-293 cells and purified via protein G columns (GE Healthcare, Munich, Germany) as described previously (71).
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bioRxiv - Immunology 2022Quote: Cells were isolated from human blood using density gradient centrifugation agent Ficoll®-Paque Premium (17-5442-02, GE Healthcare, SIGMA, Darmstadt, Germany). Monocytes were plated at 3×105 cells per well in plastic 24 well plates ...
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bioRxiv - Immunology 2023Quote: ... freshly isolated mononuclear cells were separated from human blood by density centrifugation over Ficoll-Paque Plus (GE Healthcare, Chalfont St. Giles, United Kingdom). CD4+ T cells were subsequently purified by negative selection in an Automacs (Miltenyi Biotec ...
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bioRxiv - Microbiology 2022Quote: ... The DNA was extracted using the kit Illustrative Bacteria Genomic Mini Spin Kit (GE Life Sciences, Switzerland) according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... ELISA plates were washed 3 times with PBST and 50 µL of sheep anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (GE Healthcare) was added at a dilution of 1:3,000 in blocking solution ...