Labshake search
Citations for GE Life Sciences :
51 - 100 of 5304 citations for Human G protein coupled receptor 42 GPR42 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2019Quote: ... Protein A and Protein G beads mix (1:1) (GE Healthcare) were washed with HEMG100 ...
-
bioRxiv - Cell Biology 2021Quote: ... and proteins were collected on protein G-Sepharose beads (GE Healthcare) for an additional 4 h at 4°C with gentle shaking ...
-
bioRxiv - Molecular Biology 2019Quote: ... a mixture of protein A and protein G sepharose (GE Healthcare life sciences ...
-
bioRxiv - Cell Biology 2022Quote: ... A tenth of the lysate was taken as TCE control and the rest was added to washed Protein G-coupled Sepharose beads (Sigma-Aldrich GE Healthcare 17-0618-01) for preclearing (30 min) ...
-
bioRxiv - Immunology 2023Quote: Heavy and light chain variable regions were amplified from single-cell cDNA by a nested PCR approach as previously described.[33] The PCR products were cloned into expression vectors for production of full-length human IgG1 molecules in HEK293-F cells followed by purification of the secreted mAbs on Protein G columns (GE Healthcare). Sequence analysis was done with the IMGT/V-QUEST tool.[34]
-
bioRxiv - Developmental Biology 2021Quote: ... The remaining supernatant was added to 50 µl G-Protein Sepharose beads (Protein G-Sepharose 4 Fast Flow, GE Healthcare) (pre-washed with the lysis buffer ...
-
bioRxiv - Developmental Biology 2021Quote: ... antibodies and protein G Mag sepharose (GE Healthcare) overnight at 4°C ...
-
bioRxiv - Biochemistry 2019Quote: ... 45 μl Protein G Sepharose beads (GE Healthcare) were added ...
-
bioRxiv - Molecular Biology 2019Quote: ... Protein G Sepharose beads CL-4B (GE Healthcare) were pre-equilibrated with lysis buffer ...
-
bioRxiv - Plant Biology 2020Quote: ... 50 □L of Protein G Sepharose (GE Healthcare) was used in accordance with the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... Protein G–Sepharose (GE Healthcare, 17-0618-01). Dynabeads Protein G (Invitrogen ...
-
bioRxiv - Molecular Biology 2020Quote: ... Protein G SepharoseTM 4 Fast Flow (GE Healthcare), pre-blocked with 1 mg/ml bovine serum albumin (BSA ...
-
bioRxiv - Plant Biology 2019Quote: ... 10 μl Protein G-Sepharose matrix (GE Healthcare) was added which was previously equilibrated in TBS buffer and this mixture was further incubated for another 2 h on a rotating wheel at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... Took 40μl protein G Mag Sepharose (GE Healthcare) and incubated with antibody at 4°C for 30 minutes ...
-
bioRxiv - Immunology 2022Quote: ... and Protein G Sepharose (GE Healthcare cat.# 45000118) were mixed at 1:1 ratio before adding into the supernatant and rotating overnight at 4°C ...
-
bioRxiv - Cancer Biology 2024Quote: ... and Protein G Sepharose beads (GE Healthcare, 17061801) overnight at 4 °C ...
-
bioRxiv - Immunology 2022Quote: ... The culture was harvested after seven days and the supernatant purified into PBS by Protein G affinity using a HiTrap Protein G HP affinity column (GE Healthcare), eluted with 0.1M acetic acid (VWR international ...
-
bioRxiv - Immunology 2024Quote: ... The antibody was purified from culture supernatant by protein G affinity chromatography using the ÄKTA Pure system with a HiTrap Protein G column (GE Healthcare).
-
bioRxiv - Developmental Biology 2019Quote: ... 1ml supernatant (2mg protein) was incubated with 30μl Protein G Sepharose (GE Healthcare) for two hours on a rotator at 4°C ...
-
bioRxiv - Biochemistry 2019Quote: ... Proteins were pre-cleared using 40 μl of Protein G Sepharose (GE Healthcare), for 1 hour at 4 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... The proteins were further incubated with protein A/G-sepharose beads (GE Healthcare) for 2 hours ...
-
bioRxiv - Immunology 2021Quote: ... Recombinant CEACAM6 protein consisting of the CEACAM6 extracellular domain fused to the constant region of human IgG were produced in HEK-293 cells and purified via protein G columns (GE Healthcare, Munich, Germany) as described previously (71).
-
bioRxiv - Cell Biology 2021Quote: ... GST fusion proteins were coupled to glutathione sepharose (GE Healthcare, Freiburg, Germany) using 1x buffer B (10 mM HEPES-NaOH pH 7.4 ...
-
bioRxiv - Cell Biology 2019Quote: ... One column contained total E.coli proteins covalently coupled to cyanogen bromide beads and the second column contained purified GST protein coupled to cyanogen bromide beads (GE Healthcare). Serum was cleaned by sequential incubations of 3 – 5 hours at room temperature with each column after which it was analyzed on an SDS page gel for lack of cross reactivity with GST and E.coli proteins from a total cell extract ...
-
bioRxiv - Developmental Biology 2021Quote: ... Protein G beads from GE Healthcare (GE Healthcare, 10280243) were used to purify the target protein and then the protein sample was analyzed using western blot ...
-
bioRxiv - Genomics 2020Quote: ... Protein G Sepharose beads (GE Healthcare, 4 Fast Flow) were washed with WB140 ...
-
bioRxiv - Developmental Biology 2021Quote: ... 20 μL of Protein G-sepharose beads (GE Healthcare) coated with 12 µL rabbit anti-Bicc1 ...
-
bioRxiv - Immunology 2019Quote: ... with Protein-G Sepharose (GE Healthcare, Milwaukee, WI, USA).
-
bioRxiv - Immunology 2020Quote: ... with Protein-G Sepharose (GE Healthcare, Milwaukee, WI, USA).
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... SE301 was purified using Protein G resin (GE Healthcare) in 20 mM HEPES pH 7.5 ...
-
bioRxiv - Microbiology 2023Quote: ... Protein G Sepharose (17061801) was purchased from GE Healthcare Bio-sciences AB (Pittsburgh ...
-
bioRxiv - Molecular Biology 2023Quote: ... Protein G Sepharose beads (17-0618-02, GE Healthcare) was used to recover the targeted chromatin ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the Protein A/G agarose beads (GE Healthcare) were added ...
-
bioRxiv - Genetics 2023Quote: ... Protein G Sepharose 4 Fast Flow (GE Healthcare, 17061801) was equilibrated with PBS containing 0.5% BSA ...
-
bioRxiv - Cancer Biology 2024Quote: ... Protein lysate was incubated with Protein G SepharoseTM 4 Fast Flow beads (GE Healthcare) for pre-clearance for 30 min at 4°C ...
-
bioRxiv - Developmental Biology 2021Quote: ... were coupled 2 h at RT to protein A Sepharose beads (GE Healthcare). Coupled beads were covalently crosslinked using dimethyl pimelimidate according to standard procedures ...
-
bioRxiv - Microbiology 2021Quote: ... Fifty microliters of Protein A-coupled Sepharose 4B (GE Healthcare, 17-0780-01) was preincubated for 2 h at 4 °C with 4 μg of anti-ubiquitin mouse monoclonal antibodies (Clone FK2 ...
-
bioRxiv - Microbiology 2020Quote: ... Fifty microliters of Protein A-coupled Sepharose 4B (GE Healthcare, 17-0780-01) was preincubated for 2 h at 4 °C with 4 μg of the appropriate antibody (anti-T7 epitope rabbit polyclonal antibody ...
-
bioRxiv - Molecular Biology 2019Quote: ... bead-coupled proteins were incubated with PreScission Protease (GE Healthcare, 27-0843-01) in reaction buffer (50 mM Tris-HCl ...
-
bioRxiv - Developmental Biology 2021Quote: ... were coupled overnight at 4°C to protein A Sepharose beads (GE Healthcare). Coupled beads were washed three times in EB buffer (50 mM Hepes ...
-
bioRxiv - Molecular Biology 2019Quote: ... Approximately 1mg protein was pre-cleared using 10µL of 50% protein G Beads (GE healthcare) by rotating for 15 minutes ...
-
bioRxiv - Genomics 2021Quote: ... two sets of 20 µL of Protein A or Protein G Sepharose beads (GE Healthcare) were washed three times with nuclear lysis buffer for immunoprecipitation with antibody raised in guinea pig or in rabbit respectively ...
-
bioRxiv - Immunology 2023Quote: ... the filtered supernatant ran through a protein A and protein G affinity column (GE Healthcare) and eluted with 0.2 M citric acid at pH 3.0 and neutralized in 2 M Tris-base ...
-
bioRxiv - Cell Biology 2023Quote: ... The complex was pulled down using Protein A or Protein G-sepharose beads (GE Healthcare) pre-equilibrated in lysis buffer for 2 h ...
-
bioRxiv - Immunology 2021Quote: ... supernatants were purified using Protein G Sepharose beads (GE Healthcare), dialyzed against PBS ...
-
bioRxiv - Molecular Biology 2020Quote: ... on Protein G Sepharose 4 Fast Flow beads (GE healthcare). The samples were incubated at 4°C overnight and then washed with progressively more stringent conditions ...
-
bioRxiv - Genetics 2019Quote: ... and 20 μl of protein G sepharose beads (GE Healthcare) blocked with Escherichia coli DNA and bovine serum albumin ...
-
bioRxiv - Microbiology 2021Quote: ... Protein G sepharose beads (GE Healthcare, 40 µl per IP) were equilibrated in B1 lysis buffer by washing and centrifuging them at 6,000 rpm at 4°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... conjugated to Protein G SepharoseTM 4 Fast flow (GE Healthcare) and Protein A SepharoseTM 4 Fast flow (GE Healthcare ...
-
bioRxiv - Neuroscience 2019Quote: ... antibody-coated protein G–Sepharose beads (GE Healthcare; 20 μl) were added to the mixture and incubated on a shaker overnight at 4°C ...