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Citations for GE Life Sciences :
351 - 400 of 6552 citations for Human G Protein Coupled Receptor Family C Group 6 Member A GPRC6A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Protein-G Sepharose 4 Fast Flow and SAv-Sepharose beads were obtained from GE Healthcare Bio-Sciences AB ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was precleared with beads (Protein G sepharose™ 4 flast flow, GE Healthcare) for 1 hour at 4°C on a rotating wheel ...
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bioRxiv - Microbiology 2019Quote: ... Supernatants were precleared using 20µl of protein G sepahrose beads (GE Healthcare, 7-0618-01) for 1hr at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... This solution was loaded onto a 5 ml HiTrap Protein G HP column (GE Healthcare) equilibrated with 20 mM Na3PO4 pH 7 and eluted with 0.1 M glycine-HCl pH 2.7 ...
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bioRxiv - Cell Biology 2021Quote: ... and then for another 1 h following the addition of protein-G Sepharose (GE Healthcare). Protein bound to antibody beads was washed five times with lysis buffer at 4 °C ...
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bioRxiv - Biophysics 2022Quote: ... the protein was eluted by gravity flow using a PD MiniTrapTM G-25 (GE Healthcare) Sephadex column into 200 mM HEPES ...
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bioRxiv - Molecular Biology 2020Quote: ... the antibody was pre-incubated with 20 μl of prewashed protein G sepharose (GE healthcare) at 4°C for 1 hr ...
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bioRxiv - Neuroscience 2022Quote: ... The mouse monoclonal antibodies were purified from hybridoma supernatant using protein G columns (GE Healthcare). The specificity of CAST monoclonal antibodies was confirmed by immunohistochemistry with CAST KO mice (56) ...
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bioRxiv - Neuroscience 2019Quote: ... Sera for pooled sample testing was purified using HiTrap Protein G HP columns (GE Healthcare), eluted with 0.1M glycine:HCL pH2.7 ...
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bioRxiv - Immunology 2020Quote: IgG purification was performed using HiTrap™ Protein G HP (GE Healthcare #17-0404-01) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were pre-cleared with 30 μl of protein-G-Sepharose (GE Healthcare Bio-Sciences). Pre-cleared supernatants were incubated with 5 μg of anti-SARS-CoV-2 nucleocapsid antibody mixture (GTX135357 and GTX632269 [6H3] ...
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bioRxiv - Immunology 2020Quote: ... The RBD-Fc fusion was purified from filtered supernatant on Protein G Sepharose (GE Healthcare) and cleaved using bovine enterokinase (GenScript ...
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bioRxiv - Microbiology 2020Quote: ... The RBD-Fc fusion was purified from filtered supernatant on Protein G Sepharose (GE Healthcare). The protein was cleaved using bovine enterokinase (GenScript ...
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bioRxiv - Immunology 2021Quote: ... IgG3 antibodies were isolated using a HiTrap Protein G High Performance column (Cytiva, GE Healthcare). Antibodies were dialyzed overnight in PBS and filter-sterilized though 0.22 µm Spin-X filters ...
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bioRxiv - Microbiology 2022Quote: ... whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare). Antibodies were dialyzed in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Flag immunoprecipitates of IGF-1R-Flag were incubated with Protein G Sepharose beads (GE Healthcare). Beads were pelleted ...
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bioRxiv - Immunology 2023Quote: ... the recombinant monoclonal antibodies were purified from the supernatant with protein G beads (GE Healthcare).
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were pre-cleared by incubating with protein A or G sepharose beads (GE Healthcare) for 1 h at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were purified from clarified supernatants using Protein G Sepharose 4 Fast Flow (GE Healthcare), dialyzed in PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.2% Triton X-100) and incubated 1 hour at RT or 16 hours at 4°C with HRP-coupled streptavidin (RPN1231VS, GE Healthcare) diluted 1:20000 in BSA blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... coupled with the epi-fluorescence illuminator Nikon Intensilight C-HGFIE and viral particles were purified with heparin (GE Healthcare; Cat.nr. 170406013). The concentration of the virus was checked with qPCR (fwd ITR primer ...
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bioRxiv - Microbiology 2021Quote: ... Eranthie Weerapana) for 1 h at 22 °C and desalted (PD Miditrap G-25 columns, GE Healthcare). Each light lysate was then combined 1:1 with a heavy lysate in 1 mL total volume and incubated with 2% SDS and 2 M urea final concentration in PBS in a final volume of 2 mL for 40 min at 22 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNAs encoding human NGN3 protein fragments were sub-cloned into pGEX4T-1 (GE Healthcare), expressed in Escherichia coli ...
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bioRxiv - Cancer Biology 2023Quote: The cell proliferation rate was evaluated using the Kit Biotrak ELISA System (GE Healthcare, Orsay, France) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... concentrated to 0.5 ml and after centrifugation at 21,700 g for 20 min directly applied to a Superose 6 Increase 10/300 GL column (GE Healthcare) in SEC buffer (50 mM TRIS pH 7.8 ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by centrifugation at 33,000 g and loaded on pre-equilibrated Ni-Sepharose 6 Fast Flow (HisTrap FF, GE Healthcare). The column was washed with 20-30 column volumes (CV ...
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bioRxiv - Molecular Biology 2022Quote: ... overnight at 4°C in the presence of Protein A beads (GE Healthcare). ChIP-Seq libraries were prepared using the Kapa LTP Library Preparation Kit (Kapa Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... the proteins were transferred onto a nitrocellulose membrane (Hybond-C Extra, GE Healthcare) and an anti-His-HRP conjugate antibody (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... The proteins were harvested from the supernatant of cell cultures and purified by HisTrap Excel (for his-tagged proteins) or Protein G (for antibodies) columns (GE Healthcare Life Sciences). RHMS-1 (trimer) ...
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bioRxiv - Plant Biology 2021Quote: ... Horseradish peroxidase-coupled (GE Healthcare) or alkaline phosphatase-coupled (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... was then loaded to a 5 mL HisTrap Ni-NTA column coupled to AKTA Pure protein purification system (GE Healthcare). The His-SUMO-tagged CAP1 protein was eluted from the nickel column with an imidazole gradient using buffer A and buffer B (buffer A + 250 mM imidazole) ...
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bioRxiv - Cell Biology 2021Quote: ... His⨯6-tagged proteins in the supernatant were purified by Ni-NTA agarose beads (GE Healthcare). After extensive wash by a buffer containing 20 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tagged fusion proteins were purified by Ni2+-Sepharose 6 fast flow (GE Healthcare, USA) affinity chromatography followed by size-exclusion chromatography.
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bioRxiv - Biochemistry 2019Quote: ... Plasma proteins were separated by anion exchange chromatography using Resource Q column (GE Healthcare, 6 ml) connected to an ÄKTA Avant 25 system (GE Healthcare) ...
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bioRxiv - Biophysics 2019Quote: ... the protein was subjected to separation on the Ni sepharose 6 Fast Flow resin (GE Healthcare) using the same buffers as above to separate the protein from the cleaved tag ...
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Structure of mycobacterial cytochrome bcc in complex with Q203 and TB47, two anti-TB drug candidatesbioRxiv - Microbiology 2021Quote: ... Protein was then concentrated and loaded onto a Superdex 6 increase (10/300 GL, GE Healthcare) column equilibrated in buffer B ...
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bioRxiv - Neuroscience 2022Quote: ... The eluted protein was concentrated and further injected into a Superose 6 Increase column (GE Healthcare) for size-exclusion chromatography (SEC ...
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bioRxiv - Biochemistry 2023Quote: ... the concentrated protein was applied onto a Superose 6 Increase 10/300 GL column (GE healthcare) equilibrated with SEC buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... the reaction was added to 25 µL (50 µL 50% slurry) Protein G beads (GE Healthcare) pre-coupled with antibodies ...
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bioRxiv - Cancer Biology 2019Quote: ... or a combination of the appropriate antibody and Protein A or G sepharose beads (GE Healthcare) were used to immunoprecipitate tagged or endogenous proteins from cleared lysates ...
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bioRxiv - Immunology 2022Quote: ... mAbs were harvested and purified 7 days after transfection using Protein G Sepharose columns (GE Healthcare). All purified recombinant mAbs were analyzed by SDS-PAGE under reducing conditions using NuPAGE Novex 4-12% Bis-Tris polyacrylamide gels and NuPAGE reducing agent (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... The supernatant from pDNA transfected cells was purified using Protein G GraviTrap (GE Healthcare, Chicago, IL) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Ribosomes bound to an anti-HA antibody was purified using protein G magnetic beads (GE Healthcare). The RNA bound to ribosomes was purified with RNeasy Protect Mini Kit (QIAGEN ...
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bioRxiv - Cell Biology 2022Quote: ... The homogenates were precleared by incubation with protein G Sepharose (GE Healthcare, cat#17-0618-02) for 1 hour at 4°C ...
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bioRxiv - Immunology 2022Quote: ... the supernatants consisting of secreted antibodies were collected and trapped by protein G Sepharose (GE Healthcare). The bound antibodies on the Sepharose were eluted and concentrated using Centricon Plus-70 (051555 ...
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bioRxiv - Microbiology 2022Quote: ... The supernatants were filtered and purified by HiTrap Protein G HP antibody purification columns (GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... pH 7.0) and loaded to a 1-ml HiTrap Protein G Sepharose FF column (GE Healthcare). The column was washed with binding buffer until the UV280 absorbance dropped to a stable baseline ...
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bioRxiv - Biochemistry 2019Quote: ... IP is carried out with clarified cell lysate pre-cleaned by protein G sepharose (GE Healthcare), then the cell lysate was incubated with anti-LRRC33 or control mouse IgG (2μg/ml ...
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bioRxiv - Microbiology 2021Quote: ... pH 5.0 immediately before purification on a HiTrap Protein G HP 1 mL column (GE Healthcare) using an AKTA pure instrument ...
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bioRxiv - Immunology 2020Quote: ... mAbs were purified directly from culture supernatants using HiTrap protein G columns (GE Healthcare Life Sciences) according to the manufacturer’s protocol ...