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Citations for GE Life Sciences :
401 - 450 of 1118 citations for Human Apelin 17 AP17 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... 100 µg of freeze-dried HMW compounds were dissolved in 1 mL of 20 mM sodium acetate buffer (pH 4.5) and eluted through a 1 ml HiTrap ANX FF column (GE Healthcare, 17-5162-01). Samples were eluted at a flow rate of 1 ml/min with 20 mM sodium acetate buffer pH 4.5 for 36 ml ...
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bioRxiv - Neuroscience 2019Quote: ... and 140 mM NaCl) was added and samples were pre-cleared for 1 hour using protein G sepharose (GE Healthcare, 17-0618-01). Immunoprecipitation was carried out overnight at 4 °C using anti-HA agarose beads (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... embryos were injected with mRNAs and DNAs at the four-cell stage using a PicoSpritzer setup in 1/3x MR with 2.5% Ficoll PM 400 (GE Healthcare, #17-0300-50), and were transferred after injection into 1/3x MR containing 50μg/ml Gentamycin ...
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bioRxiv - Microbiology 2022Quote: ... Protein samples were solubilized in DHHC Buffer C (1% SDS, 1 mM EDTA, 100 mM HEPES, pH 7.5) and were captured on thiopropyl sepharose 6B (GE Healthcare, 17-0402-01) in the presence of 2 M hydroxylamine or 2 M NaCl (negative control ...
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bioRxiv - Molecular Biology 2021Quote: ... Antibodies were affinity-purified from filtered cultured supernatant on a High-Trap protein G column (GE Healthcare, Chicago, USA, Cat.Nr 17-0404-01).
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bioRxiv - Cell Biology 2020Quote: ... Buffer was exchanged to BRB80 (80 mM NaPIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8) by loading protein over Nap10 (GE Healthcare, 17-0854-01) and PD10 (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... Buffer was exchanged to BRB80 (80 mM NaPIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8) by loading protein over NAP- 10 (GE Healthcare, 17-0854-01) and PD-10 (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... and the resultant lysate was incubated overnight at 4 °C with 1.5 ml of glutathione-Sepharose 4B bead slurry (GE Healthcare, 17-0756-01). Beads were washed three times in wash buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... bacterial extracts were centrifugated for 20 min at 18,000 x g at 4°C and the supernatant was incubated with 1.5 ml of a GSH-sepharose 4B resin (GE Healthcare 17-0756-01) for 1.5 h at 4°C on a rotating wheel ...
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bioRxiv - Immunology 2023Quote: ... Leukocytes were further isolated using a Percoll® gradient: samples were resuspended in 45% Percoll® (GE Healthcare, Cat. No. 17-0891-01), prepared in DMEM + 1.5M NaCl) ...
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bioRxiv - Biophysics 2023Quote: ... The cleared lysate was supplemented with CaCl2 to a final concentration of 2 mM and with KCl to a final concentration of 0.3 M and then incubated for 1 h at 4 °C with washed Sepharose 4B Calmodulin beads (GE Healthcare #17-0529-01) in a spinning rotor ...
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bioRxiv - Biochemistry 2023Quote: ... The TEV protease cleaved PIK3CB/ybbr-PIK3R1 complex was separated on a Superdex 200 size exclusion column (GE Healthcare, Cat# 17-5174-01) equilibrated with 20 mM Tris [pH 8.0] ...
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bioRxiv - Cell Biology 2023Quote: ... equal amounts of supernatant from each sample were mixed with 30 μl of pre-washed streptavidin-sepharose beads (GE Healthcare, 17-5113-01) and incubated for 3 hours at 4°C on a nutator ...
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bioRxiv - Developmental Biology 2021Quote: ... samples were red cell and granulocyte depleted by density gradient separation using Ficoll-Paque PLUS (GE Healthcare Life Sciences, cat. no. 17-5442-03) and CD34 enrichment was carried out on freshly isolated mononuclear cells (MNC ...
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bioRxiv - Molecular Biology 2021Quote: ... 300 µg of the prepared lysate was incubated with the appropriate antibody (3 µg) and pulled down using protein A Sepharose 6MB (GE healthcare # 17-0469-01). All primary antibodies used in this study are listed in table T3 ...
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bioRxiv - Immunology 2020Quote: ... The supernatant was diluted 1:1 with 20 mM sodium phosphate pH 7.4 and then purified using HisTrap Excel columns (GE Life Sciences # 17-3712-05) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The clarified extract was either directly loaded onto Superose 6 column following pre-clearing with 150 μl of Sepharose CL-2B (GE Healthcare, 17-0140-01) or subjected to Halo purification by incubating with 100 μl of HaloLink™ Resin (Promega ...
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bioRxiv - Microbiology 2021Quote: ... Purified 6x-His-SUMO or 6x-His-SUMO-Pat1 were coupled to NHS-activated Sepharose 4 fast flow resin (GE Healthcare, 17-0906-01) in ligand coupling buffer (200 mM NaHCO3 ...
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bioRxiv - Immunology 2021Quote: ... Whole blood was collected 25 to 39 days following a positive RT-PCR test and subjected to density gradient centrifugation using the Ficoll Paque Plus reagent (GE Healthcare, #17-1440-02). After separation ...
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bioRxiv - Cell Biology 2021Quote: Human peripheral blood mononuclear cells (PBMC) were isolated from whole blood by Ficoll density gradient using Ficoll Plaque Premium (Sigma GE Healthcare, #17-544-02) or BD Vacutainer CPT Tubes (BD ...
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bioRxiv - Immunology 2021Quote: ... Cells were resuspended in 10 ml FACS wash and filtered through a nylon filter before being overlayed onto 3 ml Ficoll (GE Healthcare, 17-1440-03). Cell/Ficoll mixture was centrifuged at 931g for 1 hour at room temperature with brakes off ...
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bioRxiv - Pathology 2022Quote: Epoxy-activated sepharose 4B conjugated with tegaserod maleate was prepared according to the manufacture’s instruction (#17-0480-01, GE Healthcare Life Sciences, Uppsala, Sweden). 200 μl tegaserod maleate-Sepharose 4B beads or vehicle were rotated with cell proteins (500 μg ...
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bioRxiv - Cell Biology 2022Quote: ... the remainders of the lysates were nutated with anti-Cdc15 polyclonal antibody (VU326) (Roberts-Galbraith et al., 2009) for 1 hr at 4°C followed by incubation with protein A sepharose (GE Healthcare; 17-5280-04) for 30 min at 4°C ...
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bioRxiv - Bioengineering 2019Quote: The TetR-family protein protein CAETHG_0459 was eluted in the same wash buffer containing a stepwise imidazole gradient (50-500 mM) following a buffer exchange performed using a HiTrap Desalting column (GE Healthcare #17-1408-01). Finally ...
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bioRxiv - Immunology 2020Quote: ... Whole blood was collected 31 and 32 days following a positive RT-PCR test and subjected to density gradient centrifugation using the Ficoll Paque Plus reagent (GE Healthcare, #17-1440-02). After separation ...
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bioRxiv - Molecular Biology 2020Quote: ... Immunoprecipitation procedures were performed by mixing ~1.6 mg of total proteins and 30 μl of the IgG Sepharose 6 Fast Flow beads (GE Healthcare, Cat# 17-0969-01) at 4°C for 2h and precipitating the NTAP tagged Orc4 ...
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bioRxiv - Plant Biology 2020Quote: ... Suspension was gently load onto two prepared falcon tubes containing equal volumes of 2X isolation buffer and Percoll™ (GE Healthcare-17-0891-02). Samples were centrifuged in a swing bucket centrifuge at 1200Xg for 10 minutes with brakes off ...
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bioRxiv - Cell Biology 2021Quote: ... For co-immunoprecipitation of endogenous SQSTM1/p62 the cell lysates were incubated for 2-4 h with specific antibody followed by 1-2 h with protein-G coupled Sepharose beads (GE Healthcare, 17-0885-01). To resolve protein complexes for denaturing immunoprecipitation ...
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bioRxiv - Genomics 2021Quote: ... #S0282).[1] Reverse transcription was performed in 1.5 mL microfuge tubes under end-over-end rotation using a modified Reverse Transcription mix (1x Maxima H-RT buffer, 4% Ficoll PM-400 (GE Healthcare, #17-0300-05), 3 mM MgCl2 (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... The fractions containing proteins were loaded onto the HiTrap Desalting 5 x 5 ml (Sephadex G-25 S) (GE Healthcare, Code#: 17-1408-01) with a running condition of 50 mM Tris (pH 8.0) ...
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bioRxiv - Neuroscience 2023Quote: ... The remaining tag and protease were removed by reverse Ni-NTA purification in batch mode (Ni-Sepharose High Performance; GE Healthcare, 17-5268-02). Final polishing of the target proteins was done through size exclusion on a HiLoad 26/600 Superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: White blood cells were isolated from leukocyte cones (NC24) supplied by the NHS blood and transplant service by centrifugation on Ficoll-Paque Premium (GE Healthcare 17-5442-03) for 60 min at 300 g ...
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bioRxiv - Biophysics 2023Quote: ... The cleared lysate was supplemented with CaCl2 to a final concentration of 2 mM and then incubated for 1 h at 4 °C with washed Sepharose 4B Calmodulin beads (GE Healthcare #17-0529-01) in a spinning rotor ...
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bioRxiv - Biophysics 2023Quote: ... Cdc6 protein was purified from the supernatant by incubating for 1 h at 4 °C with glutathione beads Fastflow (GE Healthcare #17-5132-02). The beads were washed 20 times with 5 ml Cdc6 lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Reduction of disulfide bonds and alkylation of cysteine residues was performed by incubating gel fragments in 10 mM 1,4-Dithio-D-threitol (GE Healthcare; cat. #17-1318-02) at 50°C for 1 hour followed by a light-protected incubation with 55 mM iodoacetamide (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... The cleaved protein was diluted to 100mM NaCl and clarified by centrifugation before application to a 5mL Heparin HiTrap HP (GE Healthcare 17-0407-01) column equilibrated with Buffer B (25mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2023Quote: ... spleens were removed and meshed through a 70 µm cell strainer in PBS and lymphocytes were isolated by Ficoll-Paque (GE Healthcare, 17-5442-03). Single cell suspensions were washed and stained for flow cytometric analysis ...
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bioRxiv - Cancer Biology 2023Quote: ... to separate blood constituents and isolation of buffy coat density gradient centrifugation (400 × g at 18°C for 30 min) of RPMI-1640 diluted blood over a Ficoll-Paque™ Plus reagent (Cat. 17-1440-02, GE Healthcare, Piscataway, NJ) was performed ...
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bioRxiv - Molecular Biology 2023Quote: ... 300 μM 5’ thiol-modified 18-nt DNA oligonucleotides—comprised of an AAA base sequence and a 15-base secondary probe binding sequence (Eurofins)—were reduced with 50 mM dithiothreitol in PBS at room temperature for 2 hours and purified using NAP5 columns (GE Healthcare 17-0853-01). Maleimide-activated antibodies were then combined with 11 equivalents of the reduced form of the thiol-modified DNA oligonucleotides in PBS and incubated at 4℃ overnight ...
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bioRxiv - Biochemistry 2020Quote: ... 150 μl of DEPC-treated water was then added and the reaction passed through a home-poured G-25 sephadex (GE Healthcare cat. # 17-0033-01) spin column ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the sections were collected on coverslips (22 mm x 22 mm) previously pretreated with a silanization solution (0.3% (v/v) bind-silane (GE Healthcare Life Sciences, 17-1330-01), 0.1% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... A tenth of the lysate was taken as TCE control and the rest was added to washed Protein G-coupled Sepharose beads (Sigma-Aldrich GE Healthcare 17-0618-01) for preclearing (30 min) ...
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bioRxiv - Biochemistry 2021Quote: ... DrrA-OCRL or DrrA-INPP5E were then loaded onto a 1 mL MonoS (5/50 GL) cation exchange column (GE Healthcare, Cat# 17-5168-01) equilibrated in 20 mM HEPES pH 7 ...
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bioRxiv - Biochemistry 2021Quote: ... Secreted protein was captured by two round of batch absorption (BA) with 4 mL/L of pre-equilibrated Ni Sepharose beads (GE Healthcare, Cat #17-5318-01). The bound beads were transferred to gravity flow column and sequentially washed with 30 CVs Wash Buffer (50 mM HEPES 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... the removal of SUMO protease and SUMO tag from the cleavage reaction was performed with affinity chromatography on a HisTrap HP column (GE Healthcare, Code#:17-5248-01). The SUMO protease and SUMO tag will stay in the HisTrap HP column and the cleaved native protein will be in the flow-through fractions ...
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bioRxiv - Neuroscience 2023Quote: ... isolation was performed by diluting sample in PBS (equivalent blood volume) and transferred on top of the Ficoll layer (GE Healthcare Cat#17-5442-02). The tubes were then centrifuged at 400xg ...
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bioRxiv - Immunology 2023Quote: ... Tissue was centrifuged at 500g for 5 minutes and resuspended in 10 mL of 30% Percoll solution (9 mL Percoll (GE Healthcare Biosciences, #17-5445-01), 3 mL 10X PBS ...
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bioRxiv - Immunology 2023Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from fresh whole blood of healthy donors by gradient centrifugation using Ficoll-PaqueTM PLUS (GE Healthcare, Cat# 17-1440-02). For T cell activation ...
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bioRxiv - Immunology 2023Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood of healthy donors by gradient centrifugation using Ficoll-PaqueTM PLUS (GE Healthcare, Cat# 17-1440-02). For T cell activation ...
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bioRxiv - Immunology 2024Quote: ... Tissue was centrifuged at 500g for 5 minutes and resuspended in 10 mL of 30% Percoll solution (9 mL Percoll (GE Healthcare Biosciences, #17-5445-01), 3 mL 10X PBS ...