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Citations for GE Life Sciences :
101 - 150 of 782 citations for Histone H3 acetyl K14 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The flow through (containing soluble free histones) was filtered with 0.22 μ filters and loaded on a cation exchange Resource S column (GE Healthcare) equilibrated with the dilution buffer EB5_1 (50 mM Tris-HCl pH8) ...
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bioRxiv - Microbiology 2020Quote: ... Human mAbs were purified by loading the supernatant onto a 1 mL Protein A HP HiTrap column (GE Healthcare). Columns were equilibrated and washed using Dulbecco’s phosphate buffered saline (DPBS) ...
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bioRxiv - Immunology 2021Quote: Kinetics binding measurement for anti-spike mAbs in this study was performed using a Biacore T200 instrument (GE Healthcare). The system was flushed with filtered 1xHBS-P+ running buffer (0.01M HEPES ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... The recombinant σ38 was firstly passed through 5 mL HisTrap HP column (GE Healthcare), and the eluted protein was diluted and incubated with purified His6-TEV protein expressed by pRK793 plasmid (Kapust et al ...
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bioRxiv - Immunology 2021Quote: ... Recombinant HA molecules were purified by FPLC using a HisTrap HP Column (GE Healthcare) and eluted with 250 mM imidazole.
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bioRxiv - Plant Biology 2020Quote: ... The recombinant proteins were purified with a pre-packaged HisTrap FF column (GE Healthcare). The His-MBP tags were cleaved by TEV protease overnight and removed by flowing through a HisTrap FF column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant proteins were purified using a Ni-NTA Superflow affinity column (GE Healthcare) and Source-Q (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... Recombinant proteins were purified by affinity chromatography using glutathione (GSH) agarose beads (GE Healthcare) and dialyzed in mild lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The eluate containing recombinant proteins was passed through a PD-10 column (GE healthcare) to exchange buffer with Store Buffer (50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Plant Biology 2021Quote: ... Recombinant proteins were immobilized on a HiTrap Talon crude 1 ml column (GE Healthcare), equilibrated with buffer A (50□mM NaH2PO4 (pH 8) ...
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bioRxiv - Neuroscience 2021Quote: ... The recombinant protein was desalted with PD-10 column (SephadexTM G-25M, GE Healthcare), purified using a cation exchange column [HiTrap carboxymethyl (CM ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant proteins were purified by IMAC using an AKTA-pure-25 FPLC (GE Healthcare). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant IgG was purified from the culture supernatants using protein A Sepharose (GE healthcare) except for Fig ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant IgG was purified from the culture supernatants using protein A Sepharose (GE healthcare). The concentration of purified IgG was measured at OD280.
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bioRxiv - Plant Biology 2023Quote: ... We purified recombinant proteins and MBP protein by a Hisprep IMAC column (GE Healthcare). Cis-fragments of target gene promoters (34-bp free probes of OsRFL containing the NRE motif and 23-bp free probes of OsMOC1 containing the WNNN (CCANTG ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were immobilized on a HiTrap Talon crude 1 mL column (GE Healthcare), equilibrated with buffer A (50 mM NaH2PO4 (pH 8) ...
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bioRxiv - Immunology 2023Quote: ... Biotinylated recombinant NS1 was purified by FPLC using a HisTrap HP Column (GE Healthcare), eluted with a 50–250 mM imidazole gradient ...
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bioRxiv - Immunology 2022Quote: ... HLA class-I and -II-associated peptides were isolated using the pan-HLA class I-specific mAb W6/32 and the pan-HLA class II-specific mAb TÜ39 (both in house mouse monoclonal) covalently linked to CNBr-activated Sepharose (GE Healthcare). HLA-peptide complexes were eluted by repeated addition of 0.2% TFA (trifluoroacetic acid ...
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bioRxiv - Immunology 2020Quote: The binding kinetics and affinity of mAbs to SARS-CoV-2 RBD were analyzed by SPR (Biacore T200, GE Healthcare). Specifically ...
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bioRxiv - Microbiology 2021Quote: ... Purified recombinant N proteins were loaded onto a Superdex 200 16/30 column (GE Healthcare) and eluted in 20 mM Tris/HCl pH 8.5 ...
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bioRxiv - Biochemistry 2019Quote: ... The recombinant protein was purified under denaturing conditions over a His-SpinTrap column (GE Healthcare), desalted using a PD SpinTrap G-25 column (GE Healthcare ...
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bioRxiv - Biophysics 2019Quote: ... Recombinant myosin was purified from clarified lysate by using the AKTA purify system (GE Healthcare). First ...
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bioRxiv - Microbiology 2019Quote: ... Purifications of the GST-tagged recombinants were performed as described by the manufacturer (GE Healthcare). For expression in mammalian cells ...
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bioRxiv - Pathology 2020Quote: ... Recombinant proteins were purified with a Ni-NTA resin and Q-Sepharose™ (GE Healthcare). Endotoxin was removed by Pierce High-Capacity Endotoxin Removal Resin (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Purified recombinant N proteins were loaded onto a Superdex 200 16/30 column (GE Healthcare) and eluted in 20 mM Tris/HCl pH 8.5 ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: ... Cleaved recombinant protein was then purified on a MonoQ 5/50 GL column (GE Healthcare) using Buffer QA (described above + 1 mM DTT ...
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bioRxiv - Immunology 2023Quote: ... the recombinant monoclonal antibodies were purified from the supernatant with protein G beads (GE Healthcare).
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bioRxiv - Neuroscience 2023Quote: ... recombinant mouse his-GST-Src was first immobilized on glutathione sepharose 4B beads (GE Healthcare). 20µg purified protein was resolved in 460µl 1xPBS and incubated with beads for 2h at 4°C with light agitation ...
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bioRxiv - Biochemistry 2023Quote: ... The recombinant proteins were batch purified on Nickel-Sepharose beads 6 Fast Flow (GE Healthcare) and eluted with 6xHis-TEV protease ...
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bioRxiv - Microbiology 2024Quote: ... and recombinant protein was purified using glutathione sepharose beads (GE Healthcare Life Sciences; Table S4).
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bioRxiv - Microbiology 2024Quote: ... α-rabbit (GE Healthcare) or α-rat (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Purification of the 4D11 mAb was carried out by affinity chromatography using a Protein A/Protein G GraviTrap 1-mL column (GE Healthcare), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: The binding and kinetic rates measurement of gp120 proteins against RV305 mAbs were obtained by surface plasmon resonance (SPR) using the Biacore 3000 instrument (GE Healthcare). SPR measurements were performed using a CM5 sensor chip with anti-human IgG Fc antibody directly immobilized to a level of 9000-11000RU (response unit) ...
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bioRxiv - Microbiology 2020Quote: ... a CM5 sensor chip was immobilized with a mouse anti-human IgG CH2 mAb using reagents and instructions supplied with the Human Antibody Capture Kit (GE Healthcare) in order to capture RBD-Fc or ACE2-Fc ...
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bioRxiv - Biochemistry 2022Quote: The binding kinetics of mAbs to SARS-CoV-2 Delta-RBD or Omicron-RBD monomer were analyzed using SPR (Biacore 8K; GE Healthcare). Specifically ...
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bioRxiv - Immunology 2021Quote: ... and mAb was purified from spent medium by affinity chromatography on an ÄKTATM pure Fast Protein Liquid Chromatography (FPLC) instrument (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... The soluble xfR5 mAb from the supernatant was isolated using HiTrap™ Protein-A HP prepacked column (GE Healthcare; NJ, USA) following standard manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... with an anti-his-tag mAb (MBL) as the primary and anti-mouse IgG HRP linked whole Ab from sheep (GE Healthcare) as the secondary antibody ...
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bioRxiv - Immunology 2021Quote: ... at room temperature for 18 hours and subsequently the mAb-spike complexes were purified using SEC (Superose 6 Increase; GE Healthcare) with TBS as a running buffer and concentrated with 10 kDa cutoff Amicon ultrafiltration units ...
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bioRxiv - Immunology 2020Quote: ... Binding of the RBD to the various mAbs was measured using single-cycle kinetics experiments with a Biacore T200 instrument (GE Healthcare). Purified mAbs were first immobilized at coupling densities of 800-1200 response units (RU ...
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bioRxiv - Immunology 2021Quote: ... to prevent activation of the alternative complement pathway and complement consumption) and anti-FH mAb (35H9) on Hi-Trap HP-NHS columns (GE Healthcare). FH protein was purified from EDTA-plasma using an anti-FH mAb (35H9 ...
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bioRxiv - Immunology 2023Quote: Heavy and light chain variable regions were amplified from single-cell cDNA by a nested PCR approach as previously described.[33] The PCR products were cloned into expression vectors for production of full-length human IgG1 molecules in HEK293-F cells followed by purification of the secreted mAbs on Protein G columns (GE Healthcare). Sequence analysis was done with the IMGT/V-QUEST tool.[34]
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bioRxiv - Biophysics 2023Quote: ... A PGT145 Ab affinity column was made by coupling PGT145 mAbs to CNBr-activated Sepharose 4B (Cat No. 170430-01, GE Bio-Sciences) and packed into a Tricorn column (GE Healthcare). The supernatant was applied over the column at 2 mL/min using an AKTA go chromatography system (Cytiva ...
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bioRxiv - Cancer Biology 2023Quote: ... the unconjugated chemical TCO/Tz click moieties were removed from the conjugated mAbs using a size exclusion column (PD-10; GE Healthcare). The conjugate solutions were further concentrated using amicon filters with a 50 kDa cutoff.
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bioRxiv - Immunology 2023Quote: ... A PGT145 and PGT151 Ab affinity column was made by coupling mAbs to CNBr-activated Sepharose 4B (Cat No. 170430-01, GE Bio-Sciences) and packed into a Tricorn column (GE Healthcare). The supernatant was applied over the column at 2 mL/min using an AKTA go chromatography system (Cytiva ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant protein was purified by chromatography using a Nickel-loaded Hitrap Chelating HP column (GE Healthcare). Unbound material was extensively washed using Tris 20mM pH8 ...
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bioRxiv - Immunology 2021Quote: Purification of the recombinant antibodies was conducted on a ÄKTAprime plus system (GE Healthcare Life Sciences) at 4°C using a 1 mL HiTrap® Protein A HP pre-packed Protein A Sepharose® column (Cytiva) ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant APE1 were then further purified by Superdex 200 increase 10/300 chromatography (GE Healthcare) in the buffer containing 20 mM Tris-HCl (pH 7.0) ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant WT and mutant INs were analyzed on Superdex 200 10/300 GL column (GE Healthcare) with running buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant protein was purified using a 1 ml HisTrap FF column (GE Healthcare, Amersham, United Kingdom) with imidazole gradient elution ...