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Citations for GE Life Sciences :
251 - 300 of 1141 citations for Herpes Simplex Virus 2 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... cell lysates of these complexes were firstly treated with GST agarose beads (GE Healthcare). And then the protein-bound beads were transferred into gravity columns and treated with 3C protease overnight at 4 °C ...
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bioRxiv - Biochemistry 2019Quote: ... The clear lysate was firstly passed through a 5 mL TALON column (GE Healthcare). After extensive washing ...
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bioRxiv - Synthetic Biology 2021Quote: ... The resulting filtered lysate was applied to a 5 mL HisTrap column (GE Healthcare) on an ÄKTA FPLC system (GE Healthcare ...
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bioRxiv - Synthetic Biology 2021Quote: ... Clarified cell lysate was loaded onto a GSTrap FF column (GE Healthcare, Piscataway, NJ) that had been pre-equilibrated with 10 column volumes of binding buffer (1x PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... Clarified lysates were loaded onto an affinity column (HisTrap HP 5 ml; GE Healthcare). Eluted protein was further purified using an anion exchange column (HiTrap Q HP 5 ml ...
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bioRxiv - Plant Biology 2020Quote: ... The clarified lysate was incubated with Ni-sepharose 6 fast flow beads (GE Healthcare) equilibrated in buffer A ...
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bioRxiv - Biochemistry 2019Quote: ... The soluble lysate was bound in batch-mode to Glutathione Sepharose 4B (GE Healthcare), then washed and eluted with 50 mM HEPES buffer pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The cleared lysate was loaded onto a pre-equilibrated HisTrap Crude FF (GE Healthcare) column ...
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bioRxiv - Biochemistry 2021Quote: ... 650μg-1mg of the lysate was added to Protein A-Sepharose beads (GE Healthcare) pre-incubated for 3-7 h with rabbit anti-FLAG antibody (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were passed through a 5 mL chelating sepharose FF column (GE Healthcare) charged with nickel and equilibrated in binding buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... the lysates were applied to Sephacryl S400 HR spin columns (GE Healthcare Life Sciences), and ribosome-protected fragments were purified using the Zymo RNA Clean & Concentrator-25 kit (Zymo Research) ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were loaded on to a Ni-sepharose IMAC gravity flow column (GE healthcare), washed with 2 column volumes of wash buffer (buffer A supplemented with 45 mM imidazole) ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleared lysate was applied to a 5 mL HisTrap HP column (GE Healthcare) and washed with Ni wash buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... the lysate of cells was loaded onto a HiTrap Chelating HP column (GE Healthcare) equilibrated with a buffer containing 20 mM imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... chromatin lysates were incubated for 3 hours with protein-G sepharose beads (GE Healthcare). Beads were washed 3× with low-salt buffer (150 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... The clarified cell lysate was first passed over Ni-NTA Sepharose resin (GE Healthcare). The resin was washed with wash buffer (lysis buffer containing 20-40 mM imidazole) ...
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bioRxiv - Cell Biology 2021Quote: ... GST fusion proteins were purified from lysates with glutathione-Sepharose 4 Beads (GE Healthcare). His-tag fusion proteins were purified from lysates using HisTrap HP column (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... His-tag fusion proteins were purified from lysates using HisTrap HP column (GE Healthcare). The size and purity of the protein preparations were verified by SDS-PAGE and Coomassie Blue staining ...
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bioRxiv - Cell Biology 2021Quote: ... The clarified lysate was added to 20 μL Glutathione Sepharose 4B resin (GE Healthcare) that had been washed three times in TUBE lysis buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... Sonicated cell lysate was loaded into a 5 ml HisTrap HP column (GE Healthcare) and purified by nickel affinity chromatography ...
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bioRxiv - Cell Biology 2022Quote: ... Clarified bacterial lysates were incubated with glutathione sepharose beads 4B (GE Healthcare Life Sciences). Beads with immobilized GST-SH3 domains were extensively washed in LB1 buffer and then added to lysates from HeLa cells ...
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bioRxiv - Plant Biology 2022Quote: ... the lysate was loaded onto amylose column MBPTrap HP (GE Healthcare, #28-9187-78) prewashed with 5 ml Column Buffer (CB) ...
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bioRxiv - Microbiology 2023Quote: ... The cell lysate was loaded on a Ni-sepharose FF HiLoad column (GE Healthcare), equilibrated in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... Extracted protein lysates were mixed with Streptavidin Sepharose High-Performance beads (GE Healthcare, USA) at 4°C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was loaded onto a HiTrap DEAE_FF 5 ml column (GE Healthcare) pre-equilibrated with 0.1 mM ZnCl2/20 mM HEPES ...
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bioRxiv - Microbiology 2023Quote: ... Clarified lysates were then incubated with A28 antisera-conjugated protein A beads (GE Healthcare) at 4 °C for 4 h with rotation at 15 rpm ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified lysates were loaded onto a 5 mL NiNTA HisTrap column (GE Healthcare). The column was then washed with 25 mL of wash buffer (4 M guanidine hydrochloride ...
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bioRxiv - Cell Biology 2023Quote: ... Lysate was circulated over 5 mL HiTrap Chelating column (GE Healthcare, Cat# 17-040901) charged with 100 mM CoCl2 for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... Cell lysate was then loaded onto glutathione SepharoseTM 4 Fast Flow resin (GE Healthcare), and washed with Tris buffer ...
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bioRxiv - Biochemistry 2023Quote: ... the cell lysates in buffer D was incubated with Ni-NTA resin (GE Healthcare), washed using buffer D supplemented with 20 mM imidazole ...
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bioRxiv - Biophysics 2023Quote: ... Clarified lysates were loaded onto a Superose 6 10/300 GL column (GE Healthcare), attached to an analytical high-performance liquid chromatography system with in-line fluorescence detection (Shimadzu) ...
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bioRxiv - Cell Biology 2023Quote: ... clarified lysates were applied to a 5 ml HisTrap™ HP column (GE Healthcare) and washed with lysis buffer followed by a buffer containing 20 mM Tris-HCl pH 8.0 at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The lysates were added to a HiTrap Chelating HP 5 ml column (GE Healthcare) equilibrated with buffer A (20 mM Hepes pH 7 ...
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bioRxiv - Molecular Biology 2024Quote: Clarified lysate was passed over a 1-ml GSTrap Fast Flow Column (GE Healthcare) equilibrated with binding/wash buffer (20 mM phosphate pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... The cleared cell lysate was loaded on a 5 mL MBPTrap column (GE Healthcare). The captured proteins were washed with 5 CV wash buffer (20 mM HEPES pH 8 ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysate was incubated with Protein G SepharoseTM 4 Fast Flow beads (GE Healthcare) for pre-clearance for 30 min at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... This lysate was applied to a nickel affinity column (HisTrap FF 5mL ©Ge healthcare) pre-equilibrated with Ni-A buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... Lysates were cleared by centrifugation and then incubated with Glutathione-Sepharose 4B resin (GE Healthcare) at 4 °C overnight and subjected to a series of salt washes (0.25M to 1M NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... This pre-clarified lysate was subjected to cation-exchange chromatography.(Sepharose fast flow GE healthcare) for further purification ...
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bioRxiv - Systems Biology 2022Quote: ... The filtered lysate was loaded onto a 1 ml HisTrap FF (GE Healthcare, Freiburg, Germany) and unbound protein was removed with 20 column volumes of 15% buffer B (50 mM HEPES/KOH pH 7.8 ...
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bioRxiv - Biophysics 2022Quote: ... Protein was affinity purified from lysate using a HisTrap HP 5 mL column (GE Healthcare) in binding buffer (50 mM NaPO4 ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates (200 μg) were incubated with 100 μL slurry of Streptavidin beads (GE Healthcare) for 1 h at RT with end-over-end mixing ...
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bioRxiv - Biochemistry 2020Quote: ... For purification of Usp21196-565 lysate was loaded ino a HisTrap FF cartridge (GE Healthcare) and eluted with 50 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... and the unclarified lysate was applied onto an IMAC HiTrap TALON crude column (GE Healthcare). The column was washed first with 50 mM sodium phosphate pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were applied to MicroSpin S-400 HR spin columns (GE Healthcare, 27-5140-01). Total RNA was purified with Zymo RNA Clean & Concentrator-25 kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2020Quote: ... Equal protein amounts of lysate were incubated with streptavidin beads (17-5113-01, GE Healthcare) for 1 hour at 4°C before being washed and analysed by western blotting.
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bioRxiv - Microbiology 2020Quote: ... SepHMs-His6 was purified from lysates using an HisTrap column in ÄKTA pure (GE Healthcare) and eluted using an increasing concentration of imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... The cleared lysate was incubated with 5 ml IgG Sepharose 6 Fast Flow (GE Healthcare) for 3 h ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was passed over a 5 ml Ni-NTA Fast Flow column (GE Healthcare) that had been equilibrated with Ni wash buffer (20 mM Tris pH8 ...
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bioRxiv - Microbiology 2019Quote: ... the lysate was loaded onto a Ni2+ column (HiPrep IMAC FF 16/10; GE Healthcare), followed by elution with a gradient of 10-250 mM imidazole in 2X PBS with 10 mM imidazole ...