Labshake search
Citations for GE Life Sciences :
601 - 650 of 1141 citations for Herpes Simplex Virus 2 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: 80 μg of primary epithelial cell lysates or 1 μg purified receptor candidates separated in SDS-PAGE gels were Western-blotted onto nitrocellulose (Amersham, GE Healthcare) with Schaeffer-Nielsen buffer (48mM Tris ...
-
bioRxiv - Molecular Biology 2019Quote: ... The remainder of the lysates were incubated with polyclonal rabbit anti-GFP (EU2; Eusera) for 1 hour followed by Protein A-Sepharose beads (GE Healthcare) for another hour at 4° C ...
-
bioRxiv - Biophysics 2019Quote: ... Cell lysate was centrifuged at 35,000 g for 60 minutes to remove insoluble cell debris and the soluble lysate was loaded onto a 5 mL His Trap HP Ni column (GE Healthcare) at a rate of 1.5 mL/minute ...
-
bioRxiv - Neuroscience 2019Quote: ... After another centrifugation and filtration step the lysate was then loaded onto a combination of a HiTrap Q and a HiTrap SP column (GE Healthcare) pre-equilibrated with running buffer ...
-
bioRxiv - Immunology 2020Quote: ... Purification of His-HMGB1 from 293-freestyle cell lysate was carried out using Ni SepharoseTM 6 Fast Flow gel (GE Healthcare). After purification ...
-
bioRxiv - Pathology 2020Quote: ... the vector was purified from the cell lysate using a iodixanol density gradient followed by anion exchange chromatography (HiTrap Q-FF column, GE Healthcare). The scAAV9-CB-SMN vector was finally resuspended and concentrated in DPBS on a centrifugal filter unit (Amicon® Ultra-15 ...
-
bioRxiv - Synthetic Biology 2019Quote: ... cells were lysed by three passes through an emulsiflex C5 (Avestin, Mannheim, Germany) and cleared cell lysate was loaded onto a HisTrap FF column (GE Healthcare). After washing with 10 column volumes of buffer A (30 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2021Quote: ... The lysates with equal total protein amount were incubated with 50 μl pre-washed Glutathione Sepharose 4B (GE Healthcare Life Sciences) in 100 μl GST pull-down buffer (20 mM Tris ...
-
bioRxiv - Microbiology 2020Quote: ... Cell debris was pelleted at 15000 × g for 10 min and 10 μg of the cleared cell lysates were resolved on a 10% SDS-PAGE and transferred to 0.45 μm nitrocellulose membrane (GE life Sciences). Membranes were blocked in blocking buffer (5% non-fat milk ...
-
bioRxiv - Microbiology 2019Quote: ... lysed with 100 μg lysozyme and sonication and the filtered cell lysate loaded onto a 1 ml HisTrap HP Ni2+ column (GE Healthcare) before elution using a gradient of Buffer B (50 mM Tris pH 7.5 ...
-
bioRxiv - Biophysics 2019Quote: ... The cells were lysed by sonication and the lysate was separated on a Ni sepharose 6 Fast Flow resin (GE Healthcare) equilibrated with 30 mM Tris-HCl pH 8 ...
-
bioRxiv - Microbiology 2020Quote: ... Supernatants from the cell lysate were run on 12% resolving Tris-Glycine gels and then transferred onto PVDF membrane (GE Healthcare). Rabbit α-strep antibody (1:1000 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cell debris was removed by centrifugation and the clear lysate was loaded onto StrepTactin Sepharose High Performance Column (StrepTrap HP, GE Healthcare). The non-specific binding protein and contaminants were flowed through ...
-
bioRxiv - Biophysics 2021Quote: ... The cells were then burst open at 19 psi using French press followed by collection of cell lysate and purification using Ni2+-NTA affinity column of AKTATM Pure (GE Healthcare). The proteins were purified using 20 mM imidazole containing binding buffer and 250 mM imidazole containing elution sodium phosphate buffer (pH 7.4) ...
-
bioRxiv - Biophysics 2020Quote: ... after which the lysate was pre-cleared for 1 h at 4 °C with 40 μl of equilibrated protein G-Sepharose beads (GE Healthcare). The day before ...
-
bioRxiv - Molecular Biology 2021Quote: ... Proteins in cell lysates were separated on SDS-PAGE and then transferred electrophoretically onto PVDF membranes (GE Healthcare, Milwaukee, WI, USA) using a transfer cell (Bio-Rad ...
-
bioRxiv - Cell Biology 2020Quote: ... centrifuged at 16,000xg for 10 min and the clarified lysates were pre-adsorbed to Protein A or Protein G beads (GE Healthcare Bioscience) for 2 hr at room temperature ...
-
bioRxiv - Biochemistry 2022Quote: ... The supernatant was first purified by Immobilized Metal Affinity Chromatography (IMAC) by loading the clarified lysate onto a 5 mL Nickel column (GE Healthcare) pre-equilibrated in buffer A ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were lysed and cleared lysate prepared as described and loaded onto a 5 mL Strep-Trap HP column (GE Healthcare), equilibrated with buffer D ...
-
bioRxiv - Plant Biology 2023Quote: ... The ASK1-D3/D3D720K (D3 or D3 with D720K mutation) complex was isolated from the soluble cell lysate by Q Sepharose High Performance resin (GE healthcare). 600 mM NaCl eluates were further subjected to Nickel Sepharose Fast Flow (GE healthcare ...
-
bioRxiv - Neuroscience 2022Quote: ... Equal volumes of cell lysates containing ∼1000 μg protein were precleared with 10 μl Protein A Sepharose (GE Healthcare, Freiburg, Germany) for 1 h at 4°C ...
-
bioRxiv - Biochemistry 2022Quote: ... The cells were disrupted by sonication and the soluble part of the lysate was purified on two 5 mL-HiTrap heparin columns (GE Healthcare) at 4 °C ...
-
bioRxiv - Biochemistry 2022Quote: ... The cells were disrupted by sonication and the soluble part of the lysate was purified on a 5 mL-HiTrap heparin column (GE Healthcare) at 4 °C ...
-
bioRxiv - Biophysics 2022Quote: ... All BtuF constructs were purified directly from whole cell lysate (obtained by sonication) via Ni-NTA chromatography followed by size-exclusion chromatography (Superdex 200 10/300 GL, GE Healthcare) in final buffers of 20-50 mM Tris-HCl ...
-
bioRxiv - Microbiology 2023Quote: ... Lysate was cleared by centrifugation and the supernatant loaded into an anion exchange column Hi-Trap Sepharose Q-HP (GE Healthcare). KtrA and KtrC were eluted with a 50-1000 mM KCl gradient ...
-
bioRxiv - Biochemistry 2023Quote: ... The lysates were cleared by centrifugation and the soluble protein fraction was affinity-purified on a HisTrap Excel column (GE Healthcare). After elution in a 0–300 mM imidazole gradient ...
-
bioRxiv - Biochemistry 2023Quote: ... Strep-tag purification (used for Rubisco-variants, see SI Table 5): Clarified lysate was applied to a 5 mL StrepTrap column (GE Healthcare) equilibrated with Strep-buffer (50 mM Tris ...
-
bioRxiv - Biochemistry 2023Quote: ... DAP5 was first purified from bacterial cell lysate using a 5 mL His-Trap HP (Ni-NTA) column from GE Healthcare Life Sciences ...
-
bioRxiv - Cell Biology 2023Quote: ... rPF4 was purified from soluble fractions of cell lysates by affinity chromatography using a 5-ml HiTrap heparin-agarose column (GE Healthcare). Endotoxin-free rPF4 was prepared as previously described 3 ...
-
bioRxiv - Cell Biology 2023Quote: ... and pure fractions were pooled and concentrated before being concentrated and snap-frozen in liquid nitrogen GST-tagged RAB7A was similarly isolated via passing cleared lysate over a gravity column packed with 5 mL of Glutathione Sepharose 4B (GE Healthcare), followed by elution using 10 mM reduced glutathione dissolved in 50 mM Tris-HCl pH 8.0. ...
-
bioRxiv - Cell Biology 2023Quote: ... The supernatant was diluted 1:1 with solubilization buffer without detergent and lysates were pre-cleared by incubation with Protein G sepharose beads (GE Healthcare) on a rotation wheel for 2 h ...
-
bioRxiv - Cell Biology 2023Quote: GST fusion protein was similarly purified using affinity chromatography by incubating bacterial cell lysate with glutathione-Sepharose 4B beads (#17075601; GE Healthcare) according to the manufacturer’s instructions and eluted from beads with elution buffer (50 mM Tris-HCl ...
-
bioRxiv - Biophysics 2022Quote: ... All BtuF constructs were purified directly from whole cell lysate (obtained by sonication) via Ni-NTA chromatography followed by size-exclusion chromatography (Superdex 200 10/300 GL, GE Healthcare) in final buffers of 20-50 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2023Quote: ... Lysate was cleared by centrifugation at 20000g for 45 min and was subjected to 5 ml His-Trap columns (GE Healthcare). The protein was eluted with a linear imidazole gradient of 15–300 mM in 30 column volumes ...
-
bioRxiv - Physiology 2023Quote: ... 2500µg of cleared mitochondrial lysate were fractionated by gel filtration using fast protein size-exclusion liquid chromatography (AKTA Pure FPLC; GE Healthcare), using a Superdex 200 Increase 10/300 column (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: ... insoluble material was removed by centrifugation at 10,000 × g and the cell lysate was pre- cleared by addition of heat inactivated goat serum and protein G sepharose beads (GE Healthcare). Proteins were then either immunoprecipitated by adding 2 μg mouse anti-GFP (Clones 7.1 and 13.1 ...
-
bioRxiv - Biochemistry 2023Quote: ... The supernatant of the cell lysate was incubated overnight at 4°C on a wheel with Glutathione Sepharose 4B resin (GSTrap 4B, GE Healthcare). After several washes ...
-
bioRxiv - Microbiology 2023Quote: ... The clarified lysate was incubated (overnight, at 4 °C, with gentle agitation) with 1 ml bed volume of GSH-Sepharose (GE Healthcare). Next ...
-
bioRxiv - Molecular Biology 2023Quote: ... DSS-treated cell lysates with ultracentrifugation and DSS-treated AcGFP were injected onto a Superdex 200 Increase (10/300GL) column (GE Healthcare), using the UltiMate 3000 UHPLC system (ThermoFisher ...
-
bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was loaded onto a pre-washed/pre-equilibrated CM Sepharose HiTrap column (5 ml, GE Healthcare, Chicago, IL) on an Äkta explorer FPLC (GE Healthcare ...
-
bioRxiv - Genomics 2023Quote: ... and the lysate were passed through a size exclusion column (Illustra MicroSpin S-400 HR Columns; GE Healthcare, 27-5140-01). RNA > 17 nt was isolated with the RNA Clean & Concentrator-25 kit (Zymo Research ...
-
bioRxiv - Molecular Biology 2024Quote: ... The cleared lysate was rotated for 1 h at 4 °C in presence of 50 µL of streptavidin sepharose (50% slurry, GE Healthcare). Beads were washed three times with NET buffer ...
-
bioRxiv - Microbiology 2024Quote: ... The lysate underwent a subsequent centrifugation at 20,000 □g for 45 □min and the cleared soluble protein lysate was loaded on a prepacked Ni-NTA column (GE Healthcare). Proteins binding to the Ni-NTA coloumns were washed with 20 column volumes of binding buffer supplemented with 20 □mM imidazole to eliminate impurities ...
-
bioRxiv - Synthetic Biology 2021Quote: ... 2 mM 2-mercaptoethanol for 12 h and purified by anion exchange chromatography (HiTrap Q, GE Healthcare) with a linear gradient of 0 to 500 mM NaCl over 40 min and size-exclusion chromatography (HiLoad 16/600 Superdex 200 ...
-
bioRxiv - Immunology 2021Quote: ... The lysate was centrifuged to remove cell debris and the supernatant was subjected to affinity chromatography using Ni-NTA beads (GE Life Sciences). The recombinant fusion proteins were eluted in 300 mM Imidazole in PBS pH 7.4while the protein was bound to the column by incubating at 4 °C for 12 to 16 h ...
-
bioRxiv - Developmental Biology 2021Quote: ... The protein lysates were first pre-cleared for 24 hours at 4°C with G-sepharose beads (GE Healthcare, Chicago, IL, USA) coupled to rabbit IgG (Bethyl Laboratories Inc ...
-
bioRxiv - Microbiology 2019Quote: ... FtsZ or ΔCTL were then purified from the lysate using anion exchange chromatography column (HiTrap Q HP 5 ml, GE Life Sciences), followed by ammonium sulfate precipitation (at 20% - 30% ammonium sulfate saturation) ...
-
bioRxiv - Biochemistry 2020Quote: ... MALX-E-NirF was purified by loading the cleared cell lysate onto a 5ml MBPtrap HP column (GE Healthcare, Chicago, United States) which was eluted with a gradient to 20 mM maltose ...
-
bioRxiv - Immunology 2020Quote: ... Lysates were centrifuged at 14,000 rpm and proteins purified by affinity chromatography using a Ni2+ column (1 mL GE Healthcare HisTrap HP) and imidazole gradient elution in an AKTA Start protein purification system ...
-
bioRxiv - Neuroscience 2023Quote: Tissue lysates were resolved by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (GE Healthcare Life Sciences, Pittsburgh, PA).