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Citations for GE Life Sciences :
51 - 100 of 2342 citations for Herpes Simplex Virus 1 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... lysates were loaded into a ResourceS (GE Healthcare) cartridge and eluted in a gradient of 0 to 500 mM NaCl in 50 mM ammonium acetate ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates were incubated with lgG sepharose (GE Healthcare) at 4 °C for 2 hrs ...
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bioRxiv - Biochemistry 2020Quote: ... Virus was purified by heparin affinity chromatography on an Akta FPLC (HiTrap, GE Healthcare), ultra-centrifuged overnight and resuspended in sterile Ringer’s solution ...
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bioRxiv - Biochemistry 2021Quote: ... The lysates were centrifuged and the supernatants used for the purification on HisTrap HP columns (1 mL; GE Healthcare) connected to an AKTA Explorer system (GE Healthcare) ...
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bioRxiv - Cell Biology 2019Quote: ... Antigens were visualized by chemiluminescence and detected using an Amersham Imager 600 (GE Healthcare Life Sciences) gel imaging system ...
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bioRxiv - Cell Biology 2022Quote: ... antibody-antigen complexes were detected by FUSION SOLO S (VILBER) using an ECL system (GE Healthcare). Quantification was performed via densitometry using Fusion Capt software (VILBER) ...
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bioRxiv - Molecular Biology 2023Quote: ... The presence of the antigens of interest was observed with Enhanced Chemifluorescence substrate (ECF, Ge Healthcare) and chemifluorescence imaging (ChemiDocTM Imaging System ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates were mixed with glutathione sepharose beads (GE Healthcare) and incubated overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Cleared lysates were incubated with IgG-Sepharose (GE Healthcare) at 4 °C for 2 h and eluted by TEV protease (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... lysates were incubated with streptavidin sepharose beads (GE Healthcare) overnight while rotating at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were incubated with Protein A Sepharose (GE Healthcare) for 1-2 hr at 4°C and bound proteins were washed extensively with lysis buffer and eluted with SDS sample buffer with boiling.
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bioRxiv - Biophysics 2019Quote: ... Lysate was loaded on to HisTrap HP (GE Healthcare), His-RanQ69L was eluted in binding buffer with 500mM Imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were loaded onto HisTrap crude column (GE Healthcare) on FPLC system (AKTA pure) ...
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bioRxiv - Biochemistry 2021Quote: ... cleared lysate was applied on GST-Trap (GE Healthcare) before extensive washing with ly-sis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Cleared lysates were incubated with IgG Sepharose (GE Healthcare), washed with buffer Y and eluted by overnight TEV cleavage.
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bioRxiv - Cell Biology 2023Quote: ... lysates were incubated with glutathione Sepharose beads (GE Healthcare) coated with the other interaction partner for 30min at 4°C on a rotary wheel ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates were incubated with Streptactin II (GE healthcare) affinity beads for 2 hours at 4°C while rotating ...
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bioRxiv - Cell Biology 2020Quote: Cells lysates were prepared using 1 % triton lysis buffer and precleared with prewashed Protein G Sepharose (PGS) beads (GE Healthcare) for 30 min on a shaker at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... The lysate was centrifuged for 1 h at 95,000g and the supernatant was loaded onto a Glutathione Sepharose HP (GE Healthcare) column ...
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bioRxiv - Microbiology 2020Quote: ... The clarified lysate was then passed using an ÄKTA Purifier FPLC through a 1 ml HisTrap HP column (GE Healthcare), pre-equilibrated in lysis buffer ...
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bioRxiv - Microbiology 2022Quote: ... the concentration of cAMP in lysates from infected THP-1 cells was determined using the cAMP enzyme immunoassay (GE Healthcare) as previously described (57) ...
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bioRxiv - Molecular Biology 2020Quote: ... The suspension was centrifuged for 10 minutes at 25,000g and the lysate supernatant was incubated for 3 hours with 1 mL of glutathione sepharose beads (GE Healthcare) pre-washed in lysis buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 mL of the clarified lysate was loaded onto a 1 mL HiTrap IMAC Sepharose FF column (GE Healthcare, Illinois) charged with Ni2+ and preequilibrated with buffer A ...
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bioRxiv - Cell Biology 2023Quote: ... Each clarified lysate was passed twice through a Ni2+-charged 1 ml HiTrap Chelating HP column (GE Healthcare Life Sciences) which had been equilibrated in Buffer TN (50 mM Tris pH 7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration in the lysates was determined by Bradford assay against BSA and 1-3mg protein-lysate equivalent was loaded onto 10μL of bead slurry (Dynabeads, GE healthcare) prewashed twice with lysis buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was clarified by centrifugation (15000 rpm for 1 h at 4 °C) and incubated with glutathione Sepharose 4B (GE Healthcare) for 4 h at 4 °C ...
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bioRxiv - Microbiology 2020Quote: 80 μg of primary epithelial cell lysates or 1 μg purified receptor candidates separated in SDS-PAGE gels were Western-blotted onto nitrocellulose (Amersham, GE Healthcare) with Schaeffer-Nielsen buffer (48mM Tris ...
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bioRxiv - Molecular Biology 2019Quote: ... The remainder of the lysates were incubated with polyclonal rabbit anti-GFP (EU2; Eusera) for 1 hour followed by Protein A-Sepharose beads (GE Healthcare) for another hour at 4° C ...
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bioRxiv - Microbiology 2019Quote: ... lysed with 100 μg lysozyme and sonication and the filtered cell lysate loaded onto a 1 ml HisTrap HP Ni2+ column (GE Healthcare) before elution using a gradient of Buffer B (50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... after which the lysate was pre-cleared for 1 h at 4 °C with 40 μl of equilibrated protein G-Sepharose beads (GE Healthcare). The day before ...
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bioRxiv - Microbiology 2023Quote: ... The clarified lysate was incubated (overnight, at 4 °C, with gentle agitation) with 1 ml bed volume of GSH-Sepharose (GE Healthcare). Next ...
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bioRxiv - Molecular Biology 2024Quote: ... The cleared lysate was rotated for 1 h at 4 °C in presence of 50 µL of streptavidin sepharose (50% slurry, GE Healthcare). Beads were washed three times with NET buffer ...
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bioRxiv - Bioengineering 2020Quote: ... Clarified lysate loaded onto HiTrap Q HP column (GE Healthcare) and eluted through salt gradient using low-salt buffer (as described above ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysates were loaded on HisTrap HP column (GE Healthcare) and after extensive washing the recombinant proteins were eluted by imidazole gradient ...
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Structural determinants of protocadherin-15 elasticity and function in inner-ear mechanotransductionbioRxiv - Biophysics 2019Quote: ... The cleared lysates were loaded onto Ni-Sepharose (GE Healthcare), eluted with denaturing buffer supplemented with 500 mM imidazole and refolded as indicated in Table S3 using MWCO 2000 membranes (Spectra/Por) ...
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bioRxiv - Biophysics 2019Quote: ... The lysate was purified by Histrap FF columns (GE Healthcare) with an imidazole gradient from 0 to 0.5 M ...
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bioRxiv - Cell Biology 2020Quote: ... Centrifuge-cleared lysate was applied to Ni sepharose (GE healthcare), washed with 50 mM HEPES (pH 7.4) ...
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bioRxiv - Plant Biology 2021Quote: ... protein lysates were incubated with glutathione Sepharose beads (GE Healthcare) in E buffer for 2 h ...
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bioRxiv - Cell Biology 2020Quote: ... Clarified lysates were incubated with prewashed glutathione sepharose (GE Healthcare) and gently mixed with rotation for 1 h ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates were mixed with protein G sepharose beads (GE Healthcare) bound to α-PP2A-Aα or control IgG ...
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bioRxiv - Molecular Biology 2021Quote: ... the cleared lysate was applied to Streptactin beads (GE Healthcare) in batch and incubated for 1 h at 4°C with rotation ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates were incubated with IgG sepharose 6 beads (GE Healthcare) in XB buffer (100 mM KCl ...
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bioRxiv - Biophysics 2019Quote: ... Cleared lysate was loaded onto a glutathione-sepharose (GE Healthcare) column (GST-column ...
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bioRxiv - Microbiology 2021Quote: ... Cleared lysates were incubated with glutathione Sepharose 4B (GE Healthcare), washed with wash buffer (20 mM Tris pH 8.5 [pUL21-GST] or 7.5 [GST-PP1γ] ...
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bioRxiv - Biochemistry 2021Quote: ... cleared lysate was incubated with glutathione-Sepharose resin (GE Healthcare) for several hours before washing the resin first with PBS buffer containing 1% (v/v ...
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bioRxiv - Microbiology 2020Quote: ... Cell lysates were mixed with either StrepTactin sepharose (GE Healthcare) for PorEStrep immunoprecipation ...
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bioRxiv - Microbiology 2019Quote: ... Cleared lysates were then incubated with glutathione sepharose (GE healthcare) for 4 hours at 4°C and ...
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bioRxiv - Microbiology 2021Quote: ... The lysates were loaded on HisTrap HP column (GE Healthcare) and after extensive washing the recombinant protein was eluted by imidazole gradient ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were incubated with pre-equilibrated streptavidin beads (GE Healthcare) for 4 hr ...
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bioRxiv - Neuroscience 2023Quote: ... The cleared lysates were loaded onto Ni-Sepharose (GE Healthcare) and eluted with denaturing buffer or non-denaturing buffer supplemented with 500 mM imidazole ...