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Citations for GE Life Sciences :
301 - 350 of 1780 citations for Haptoglobin HP Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: His-purified CsoSCA-MBP-his was run on a Superose 6 Increase 10/300 GL size exclusion column (GE Healthcare) to remove MBP-his contamination ...
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bioRxiv - Microbiology 2019Quote: ... and anti-His (GE Healthcare, Chicago, IL, USA) primary antibodies ...
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bioRxiv - Immunology 2021Quote: ... A Hi-Trap® desalting column (GE Healthcare), equilibrated with argon-flushed 100 mM sodium phosphate pH 7.4 buffer ...
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bioRxiv - Immunology 2021Quote: ... A Hi-Trap® desalting column (GE Healthcare), equilibrated with 20 mM sodium phosphate buffer pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... His-tag was cleaved by thrombin (GE Healthcare) and NaCl concentration was reduced to 100 mM ...
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bioRxiv - Biophysics 2024Quote: ... loaded onto a His-Trap column (GE Healthcare), and eluted by applying a linear imidazole gradient from 20 mM to 200 mM over 30 column volumes ...
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bioRxiv - Plant Biology 2024Quote: ... purified on a His-Trap column (GE Healthcare) following the manufacturers’ instructions ...
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bioRxiv - Biophysics 2019Quote: ... TtRecFS261C and TtRecO were purified using HiTrap Heparin HP column (GE healthcare). All the purified proteins were further polished using gel filtration column (Hi-load 16/600 Superdex200pg ...
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bioRxiv - Cell Biology 2020Quote: ... Nucleotides were removed with a Heparin column (HiTrap Heparin HP, GE Healthcare) by binding protein in 250mM NaCl and isocratic elution in 750mM NaCl ...
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bioRxiv - Microbiology 2019Quote: ... lysate was applied to a 5 ml HisTrap HP column (GE healthcare) in buffer containing 25 mM Tris/HCl ...
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bioRxiv - Microbiology 2019Quote: ... and loaded onto 1 mL MBP Trap™ HP column (GE Healthcare) following equilibration with Buffer A ...
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bioRxiv - Cell Biology 2019Quote: ... Clarified protein extracts were loaded onto a HisTrap HP column (GE Healthcare) pre-equilibrated in 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2019Quote: ... and then passed over a 5 mL HisTrap HP column (GE Healthcare). The column was washed with 10 column volumes (CV ...
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bioRxiv - Microbiology 2019Quote: ... lysate was applied to a 5 ml HisTrap HP column (GE healthcare) and washed with buffer containing 25 mM Tris/HCl ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was loaded onto a 5-mL StrepTrap HP (GE Healthcare) column and then washed with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2019Quote: ... and HiTrap™ IgM Purification HP columns (GE Healthcare, Wauwatosa, WI, USA), respectively ...
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bioRxiv - Biophysics 2021Quote: ... The TEV reaction was passed through a HisTrap HP column (GE Healthcare) as a subtractive step to remove His6-TEV protease ...
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bioRxiv - Cell Biology 2020Quote: ... filtered and loaded onto a HisTrap HP 5 ml column (GE Healthcare). After extensive washes with wash buffer (50 mM Hepes pH 8 ...
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bioRxiv - Biochemistry 2020Quote: ... in brief: lysates were loaded into a GSTrap HP cartridge (GE Healthcare) and treated overnight with GST-PreScission Protease (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... and UbcH7 lysates were loaded into a GSTrap HP cartridge (GE Healthcare); once loaded GST-PreScission Protease (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were purified using a 5 mL HisTrap HP column (GE healthcare) followed by gel filtration (Superdex 30/60 GE healthcare).
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bioRxiv - Neuroscience 2021Quote: ... and subsequently loaded on a HisTrap HP column (GE Healthcare Life Sciences) using 25 mM sodium phosphate ...
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bioRxiv - Biophysics 2020Quote: ... dialyzed sample was applied on a HiTrap HP Q column (GE Healthcare) equilibrated in buffer QA and eluted in a gradient with increasing concentrations of NaCl using buffer QB (40 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... PCR products were bound to Streptavidin Sepharose HP (GE Healthcare Life Sciences) for immobilization ...
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bioRxiv - Biochemistry 2021Quote: ... and loaded onto a Q-HP anion exchange (AEX) column (GE Healthcare) equilibrated with dilution buffer containing 0.1 mg/mL DDM ...
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bioRxiv - Biochemistry 2021Quote: ... Lysate was applied to a 5 ml HisTrap HP column (GE Healthcare) equilibrated in Buffer A (20 mM HEPES-KOH ...
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bioRxiv - Biochemistry 2021Quote: ... HCoV-OC43 S was purified using a StrepTrap HP column (GE healthcare). Purified proteins were concentrated ...
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bioRxiv - Biochemistry 2021Quote: ... and loaded on an anion-exchange HiTrap Q HP column (GE Healthcare). Next ...
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bioRxiv - Biochemistry 2020Quote: ... bound to Ni2+ ions followed by a StrepTrap HP column (GE healthcare). Elution from the chelating resin was performed using 250mM imidazole pH 8.0 added to the Lysis buffer and allowed the protein to bind to the StrepTactin Sepharose resin ...
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bioRxiv - Biochemistry 2020Quote: ... loaded on a 20 ml Ni-IMAC Sepharose HP column (GE Healthcare) and after several washing steps RNAP was eluted into IMAC elution buffer (IMAC binding buffer containing 250 mM imidazole) ...
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bioRxiv - Synthetic Biology 2021Quote: ... HP columns (1 mL) (both GE Healthcare Life Sciences, Little Chalfont, UK). A buffer exchange was carried out to remove excess imidazole using an Amicon® Ultra-15 Centrifugal Filter Unit – 3 kDa MW cutoff (UFC900308 ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was directly applied to a HisTrap HP column (GE Healthcare), equilibrated with binding buffer ...
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bioRxiv - Microbiology 2021Quote: ... StrepII-CERT was captured using 1 mL StrepTrap HP column (GE Healthcare) that had been pre-equilibrated in wash buffer (100 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... and loaded onto a 1-mL HiTrap IMAC-HP column (GE Healthcare), which was pre-equilibrated with lysis buffer containing 0.1% dodecyl maltoside ...
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bioRxiv - Biophysics 2022Quote: ... hydrophobic chromatography on a phenyl-sepharose column (HiTrap Phenyl HP, GE Healthcare) and size exclusion chromatography on an analytical HiLoad 16/60 Superdex 75 (HiLoad 16/600 Superdex 75 pg ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was loaded onto HisTrapTM HP 5 ml column (GE healthcare) and eluted with buffer A containing 300 mM imidazole on an ÄKTA purifier system (GE Healthcare) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Supernatant was applied to a 5 ml HisTrap HP column (GE Healthcare) using an AKTA Prime liquid chromatography system before washing with buffer B (20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was loaded onto a HisTrap HP column (GE Healthcare, USA). Proteins were purified by fast protein liquid chromatography and washed using two column volumes of Ni Buffer A (50 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... proteins were additionally purified by passing through HisTrap HP column (GE Healthcare). Size exclusion chromatography on a Superdex 75 10/300 column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... The recombinant proteins were purified on a HisTrap HP column (GE Healthcare), followed by buffer exchange on a HiPrepTM 26/10 desalting column (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were finally loaded on a HisTrap HP Chelating column (GE healthcare) in Tris/NaCl buffer supplemented with 40 mM imidazole and eluted with 150 mM imidazole ...
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bioRxiv - Biophysics 2022Quote: ... The protein solution was applied to a Phenyl-sepharose HP (GE Healthcare) column equilibrated with 20 mM MES-NaOH pH 6.0 ...
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bioRxiv - Biophysics 2019Quote: ... Nucleotides were removed with a Heparin column (HiTrap Heparin HP, GE Healthcare) by binding protein in 250mM NaCl and isocratic elution in 750mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... and loaded on 1 mL HiTrap Heparin HP (GE Healthcare, Cat# 17040601) equilibrated with buffer C (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... and loaded onto a 5 ml HiTrap Chelating HP column (GE Healthcare) preloaded with NiSO4 as described by the manufacturer and pre-equilibrated in wash buffer (50 mM NaH2PO4 ...
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bioRxiv - Biochemistry 2019Quote: ... A HiTrap SP HP column (5 ml) (17-1151-01, GE Healthcare) was equilibrated with buffer A containing 0.1 M KCl prior to loading the clarified lysates ...
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bioRxiv - Biochemistry 2019Quote: ... with an anion-exchange HiTrap Q HP column (1 mL, GE Healthcare) using a salt gradient with a start and end concentration of 100 and 500 mM NaCl in 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were further purified by HiTrap HP cation exchange chromatography (GE Healthcare) and Superdex75 gel filtration chromatography (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... Eluted material was further purified by a HiTrap Q-HP (GE Healthcare) anion exchange column ...
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bioRxiv - Microbiology 2021Quote: ... RBD-SD1was purified by HiTrap Protein G HP column (GE Healthcare, US) pre-equilibrated in PBS and eluted with 0.1 M glycine (pH 2.7) ...