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Citations for GE Life Sciences :
351 - 400 of 5650 citations for Guanine Nucleotide Binding Protein G Protein Alpha Activating Activity Polypeptide O GNAO1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... IgG and IgM antibodies were purified from plasma samples by affinity chromatography using prepacked HiTrap™ Protein G HP columns (GE Healthcare, Wauwatosa, WI, USA) and HiTrap™ IgM Purification HP columns (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... IgG and IgA antibodies were depleted from plasma obtained from 25 recovered COVID-19 patient using Protein G HP Spintrap (GE Healthcare Life Sciences, Buckinghamshire, UK) and Peptide M / Agarose (InvivoGen ...
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bioRxiv - Cell Biology 2021Quote: ... polypeptides of interest were visualized with the enhanced chemiluminiscence system (ECL, GE Healthcare). Total protein on blots was stained with Simply Blue Colloidal Coomassie reagent (Invitrogen).
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bioRxiv - Biochemistry 2022Quote: ... Protein (GE Healthcare), according to the manufacturer’s specifications in protocol “E,” except that glutardialdehyde (25% w/v ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of protein from each sample were added to 50 µl of precleaned CaM binding beads (GE Healthcare, 17-0529-01) in 300 to 500 µl of 1% TX100-TNM lysis buffer and incubated with rotation at 4°C overnight (16-18 hours) ...
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bioRxiv - Microbiology 2020Quote: ... IgG and IgM were captured by affinity chromatography using HiTrap Protein G High Performance column (GE Healthcare) and CaptureSelect IgM Affinity Matrix (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant containing 2F2 or 2E10.E9 IgG was purified via Protein G affinity chromatography (GE Healthcare) and size-exclusion chromatography (Superose 6 Increase 10/300 GL ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were collected from selected hybridoma clones and purified through a protein G-sepharose column (GE Healthcare). mAbs were isotyped with Pierce rapid isotyping kit (Cat#26178) ...
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bioRxiv - Molecular Biology 2019Quote: ... the reaction was added to 15 µL (30 µL 50% slurry) Protein A:Protein G beads (GE Healthcare) pre-coupled with antibodies ...
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bioRxiv - Immunology 2019Quote: ... followed by the addition of 30 μl of a 50% slurry of protein G beads (GE Healthcare), and incubation overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Fractions containing the purified protein were pooled and desalted with PD midi Trap G-25 (GE Healthcare) using the spin protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Immune complexes were isolated by the addition of 20 μL of protein G– Sepharose beads (GE Healthcare) followed by incubation for 2 hr at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Chromatin samples derived from 1.0×106 cells were precleared with protein G sepharose (GE Healthcare, Chicago, IL) and then 1-5 μg of each antibody was added to form immune complex ...
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bioRxiv - Microbiology 2020Quote: IgG was purified from heat-inactivated human plasma using Protein G HP spin trap columns (GE Healthcare) and then incubated with CaptureSelect IgA Affinity Matrix (ThermoScientific ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were either stained with Coomassie brilliant blue G-250 or transferred to nitrocellulose membranes (GE Healthcare). The membranes were blocked with 5% milk in 0.05% Tween TBS at room temperature for 1 h and then incubated at 4 °C overnight with a specific polyclonal serum produced by immunization of mice with RBD produced in HEK-293T cells ...
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bioRxiv - Microbiology 2019Quote: ... Dual-labeled capsid proteins were separated using a PD Mini-Trap G-25 desalting column (GE healthcare) with imaging buffer (50 mM Tris ...
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bioRxiv - Immunology 2020Quote: ... Sera were then pooled for each group and IgGs were purified using protein G beads (GE Healthcare). The IgG concentration for each sample was obtained measuring the absorbance at 280 nm using a nanodrop (Thermo Scientific).
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bioRxiv - Immunology 2021Quote: ... Anti-DEC205-OVA was purified from the media using a HiTrap Protein G HP column (GE Healthcare) according to the manufacturer’s recommendation ...
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bioRxiv - Microbiology 2022Quote: Total IgG was purified from the final bleed rabbit sera using Protein G Gravitrap (GE Healthcare, Cytiva). The sera were diluted as 1:5 in binding buffer (20 mM Sod ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were revealed using the appropriate HRP-conjugated secondary antibodies (GE healthcare). Proteins were then identified using Immobilon Western (Millipore ...
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bioRxiv - Immunology 2020Quote: ... antibodies were immobilized on Series S Protein L sensor chip (GE Healthcare) at a density of 400 RU and experiments were performed in HBS-EP+ buffer at pH 6.0 following the method described above ...
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bioRxiv - Molecular Biology 2021Quote: ... Antibody-chromatin complexes were immobilized on Protein A-Sepharose beads (GE Healthcare) for 16 h at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were detected with HRP-conjugated secondary antibodies (1:10,000; GE Healthcare) and visualized using an AI600 Imager (GE Healthcare) ...
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bioRxiv - Immunology 2022Quote: ... and antibody was purified by Protein A affinity chromatography column (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... reactions were ended with 30 mM EDTA and unincorporated nucleotides were removed using Illustra MicroSpin G-50 columns (GE Healthcare). Samples were then separated on 0.6% alkaline agarose gels (30 mM NaOH and 2 mM EDTA ...
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bioRxiv - Cell Biology 2019Quote: ... Immunoprecipitated proteins were pulled down with protein A-sepharose (GE Healthcare) for immunoblotting ...
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bioRxiv - Bioengineering 2020Quote: ... Fc-Fusion proteins were isolated using HiTrap protein-A (GE Healthcare) affinity columns.
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bioRxiv - Immunology 2022Quote: ... and stirred overnight (O/N) at 4°C with 4.5 g CM Sephadex C-25 beads (GE Healthcare) preequilibrated for 3 h with 100 mL of buffer A ...
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bioRxiv - Immunology 2020Quote: ... Antibody binding was detected using the ECL system (GE Healthcare). Images were acquired on film (Kodak ...
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bioRxiv - Genetics 2023Quote: ... Antibody binding was detected using the ECL system (GE Healthcare). Images were acquired on G:BOX gel imaging system (Syngene ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were purified from the infected cell culture supernatants by binding to a HiTrap ProteinG HP column (GE Healthcare Life Sciences, Piscataway, NJ) and eluted with 0.1 M citrate ...
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bioRxiv - Immunology 2021Quote: ... fritted deep-well plates were used containing 25 μL of settled protein G resin (GE Healthcare Life Sciences) per well ...
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bioRxiv - Immunology 2021Quote: ... fritted deep-well plates were used containing 25 μl of settled protein G resin (GE Healthcare Life Sciences) per well ...
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bioRxiv - Microbiology 2020Quote: ... Eight days post transfection the supernatant was loaded on the HiTrap protein G HP 1ml column (GE Healthcare) in binding buffer (20 mM sodium phosphate pH 7.0 ...
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bioRxiv - Neuroscience 2022Quote: ... The lysate was divided equally and incubated with 50 μl of prewashed 50% Protein G slurry (GE Healthcare) for each condition ...
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bioRxiv - Microbiology 2019Quote: ... The elution fractions containing the purified protein were loaded onto a PD MidiTrap G-25 column (GE healthcare) equilibrated with 20 mM phosphate buffer (pH 7.4 ...
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bioRxiv - Developmental Biology 2020Quote: ... Lysates were precleared using 20 μL protein G Sepharose 4 fast flow (1:1 in PBS, GE Healthcare) for 30 minutes at 4°C on a shaker ...
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bioRxiv - Immunology 2020Quote: ... After 6 days the supernatant was harvested and Protein G Sepharose® 4 Fast Flow beads (GE Healthcare) were used for antibody purification ...
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bioRxiv - Microbiology 2020Quote: ... All mAbs were purified from cell culture supernatant using a HiTrap protein G HP affinity column (GE Healthcare) and stored at −80 °C ...
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bioRxiv - Immunology 2021Quote: ... After 6 days the supernatant was harvested and Protein G Sepharose® 4 Fast Flow beads (GE Healthcare) were used for antibody purification.
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bioRxiv - Immunology 2020Quote: ... we used fritted deep well plates containing 25 μL of settled protein G resin (GE Healthcare Life Sciences) per well ...
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bioRxiv - Immunology 2020Quote: ... fritted deep-well plates were used containing 25 μl of settled protein G resin (GE Healthcare Life Sciences) per well ...
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bioRxiv - Immunology 2022Quote: The total IgG was purified from murine serum using Protein G Sepharose 4 Fast Flow columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Immunoprecipitation was performed by incubating sheared chromatin with Protein G Sepharose™ 4 Fast Flow beads (GE Healthcare) and antibodies ...
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bioRxiv - Microbiology 2023Quote: ... The immunocomplex was captured by using protein G Sepharose 4 fast flow beads (17-0618-01/ GE Healthcare). The immunoprecipitated complex was washed two times with IP lysis buffer and one time with mili-Q ...
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bioRxiv - Neuroscience 2024Quote: ... The immuno-complexes were then incubated with 30 μl protein G sepharose beads (GE healthcare, 17-0618-01), which were pre-equilibrated with Lysis Buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 μl of a protein A/G sepharose mixture (GE Healthcare; #17-0780-01 and #17-0618-01), pre-equilibrated in ChIP dilution buffer was added to the lysates and incubation continued for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... fritted deep-well plates were used containing 25 μl of settled Protein G resin (GE Healthcare Life Sciences) per well ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were then incubated with washed and pre-blocked protein G Sepharose beads (50 µl, GE Healthcare) for 45 mins at 4°C ...
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bioRxiv - Biophysics 2019Quote: ... polypeptides were refolded through a size exclusion column Superdex200 10/300 increase (GE Healthcare) in the buffer (20 mM Tris-HCl pH 8.0 ...