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Citations for GE Life Sciences :
701 - 750 of 1736 citations for Glutathione S transferase Class mu 26 kDa Isozyme GST Schistosoma Japonicum since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The supernatant was loaded on to pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare) and recombinant proteins were eluted by different concentrations of glutathione (5-40 mM) ...
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bioRxiv - Cell Biology 2021Quote: ... The clarified lysate was added to 20 μL Glutathione Sepharose 4B resin (GE Healthcare) that had been washed three times in TUBE lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... and His(6)GFP –AnkBD proteins were purified using Glutathione Sepharose 4B (GE Healthcare) or Talon® (Clontech ...
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bioRxiv - Cell Biology 2022Quote: ... Clarified bacterial lysates were incubated with glutathione sepharose beads 4B (GE Healthcare Life Sciences). Beads with immobilized GST-SH3 domains were extensively washed in LB1 buffer and then added to lysates from HeLa cells ...
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bioRxiv - Cell Biology 2023Quote: ... coli extracts on 100 μl Glutathione–Sepharose beads in PBS (GE Healthcare, Freiburg, Germany). After incubation for 1h at 4°C with either E.coli extracts or purified proteins ...
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bioRxiv - Plant Biology 2023Quote: ... Tagged proteins were purified separately using Glutathione Sepharose 4B (17075601, GE Healthcare, Chicago, USA) or Ni-NTA affinity agarose beads (30210 ...
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bioRxiv - Biochemistry 2023Quote: ... HisGST-CK1δ ΔC fusion proteins were purified using Glutathione Sepharose 4B resin (GE Healthcare) using standard approaches and eluted from the resin using 50 mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: ... Cell lysate was then loaded onto glutathione SepharoseTM 4 Fast Flow resin (GE Healthcare), and washed with Tris buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... The total proteins were isolated and then purified using glutathione Sepharose 4B (GE Healthcare), Ni Sepharose (GE Healthcare ...
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bioRxiv - Biophysics 2019Quote: ... This mixture was then filtered through a 0.2 μM filter and concentrated on a 10 kDa (for EC1-5/EC3-5 proteins) or a 100 kDa (for full length proteins) MWCO column (Vivaspin, GE Healthcare). The protein was buffer exchanged 2-3x using either 7 kDa MWCO Zeba Columns for EC1-5/EC3-5 proteins or 40 kDa MWCO Zeba Columns for full length proteins (ThermoFisher).
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bioRxiv - Biochemistry 2021Quote: ... Soluble protein was injected on a HiLoad Sephadex 16/600 S200 (for constructs larger than ~25 kDa) or S75 (for constructs smaller than ~25 kDa) (GE Healthcare) equilibrated in SEC buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2019Quote: ... or Superdex S-200 (GE Healthcare) in 10/300 GL columns were used on NGC chromatography systems ...
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bioRxiv - Neuroscience 2020Quote: ... MicroSpin S-400 columns (GE Healthcare) were used to isolate ribosome bound RNAs ...
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bioRxiv - Immunology 2022Quote: ... Series S Sensor SA (GE Healthcare) chips were primed in 10 mM HEPES ...
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bioRxiv - Molecular Biology 2020Quote: ... Sephacryl S-400 columns (GE Healthcare) were used ...
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bioRxiv - Biochemistry 2021Quote: ... followed by two consecutive gel filtration steps (HiLoad 26/600 Superdex 200 pg; GE healthcare), the first in buffer A (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2020Quote: ... and injected on pre-equilibrated Superdex HiLoad S200 16/60 or 26/600 (GE Healthcare) at 4°C with lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were grown for 26-30 hours before scanning on a Typhoon FLA9500 (GE Healthcare) fluorescence scanner equipped with 488 nm and 532 nm excitation lasers and 520/40 and 610/30 emission filters ...
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bioRxiv - Biophysics 2019Quote: ... cZP1-N1 was injected into a HiLoad 26/600 Superdex 200 pg column (GE Healthcare) pre-equilibrated with 20 mM Na-HEPES pH 8.0 ...
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bioRxiv - Biophysics 2019Quote: ... Proteins were then loaded onto a SEC HiLoad Superdex 75 26/60 column (GE Healthcare) that was equilibrated with 40 mM phosphate buffer ...
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bioRxiv - Cell Biology 2019Quote: ... eluted with imidazol and desalted on silica column with PBS (HiPrep 26 /10Desalting, GE Healthcare) or on a Vivaspin column (Vivaspin Sartorius) ...
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bioRxiv - Synthetic Biology 2020Quote: ... column and then a size exclusion column (HiLoad 26/60 Superdex 200 increase GE Healthcare) before concentration and storage at −80 °C.
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bioRxiv - Neuroscience 2020Quote: ... This sample was run through Hiload 26/600 Superdex 200pg (GE Healthcare, 28-9893-36) column equilibrated with PBS and eluted as 2.5 mL fractions at a flow rate of 1 mL/min ...
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bioRxiv - Biochemistry 2020Quote: ... and further purified by gel filtration on a Superdex 75 26/60 column (GE Healthcare) using 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... followed by size-exclusion chromatography (HiLoad™ 26/600 Superdex™ 200 pg, GE Healthcare) in buffer (30 mM Tris ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein was further polished on XK 26/70 Superose 6 pg column (GE Healthcare) equilibrated in 20 mM Tris-HCl pH 8 (4 °C) ...
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bioRxiv - Biophysics 2021Quote: ... The following day imidazole was removed using a HiPrep 26/10 Desalting Column (GE Healthcare) preequilibrated with buffer A ...
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bioRxiv - Microbiology 2021Quote: ... followed finally by size-exclusion chromatography on a 26/60 superdex 200 column (GE Healthcare). At each stage ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was recovered and passed over a Superdex 200 26/60 column (GE Healthcare) equilibrated with 6 M GdmCl ...
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bioRxiv - Molecular Biology 2019Quote: ... The complex was run on a Superdex 200 26/60 column (GE Healthcare Life Sciences) in 50 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... FANCI was then run on a Superdex 200 26/60 column (GE Healthcare Life Sciences) in 50 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... FANCD2 was then run on a Superdex 200 26/60 column (GE Healthcare Life Sciences) in 50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted protein was applied to a HiLoad Superdex 75 26/600 column (GE Healthcare) equilibrated with SEC Buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: ... 10 mM Imidazole (pH 8.0)) using a Hi-Prep Desalting 26/10 column (GE Healthcare). To remove the HIS-myc affinity tag ...
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bioRxiv - Immunology 2021Quote: ... then further purified by size exclusion chromatography with Superdex 200 26/60 column (GE Healthcare) with the protein storage buffer (500 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... and loaded onto a Sephacryl S100 HR 26/60 size-exclusion chromatography column (GE Healthcare), with a mobile phase adequate to the downstream use of the protein ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were further purified by size-exclusion chromatography (Superdex-200 26/60, GE healthcare) with the buffer containing 150mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... the protein sample was passed over a HiLoad 26/600 Superdex 200 column (GE Healthcare) pre-equilibrated with the equilibration buffer (100 mM NaCl ...
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bioRxiv - Synthetic Biology 2023Quote: ... and subjected to size exclusion chromatography (SEC) using a XK 26/100 column (GE Healthcare) equilibrated with 50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted MsbA was pooled and desalted using HiPrep 26/10 desalting column (GE Healthcare) pre-equilibrated in desalting buffer (NHA-DDM with imidazole omitted) ...
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bioRxiv - Cell Biology 2019Quote: GST-tagged mammalian ATG8 fusion proteins were cloned into pGEX-4T-1 (GE Healthcare) and expressed in Escherichia coli BL21 (DE3 ...
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bioRxiv - Biochemistry 2021Quote: ... The GST affinity tag was then cleaved using the PreScission Protease (GE Life Sciences) for four hours in cleavage buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was incubated with 5mL of washed and packed GST resin (GE Healthcare) for 1 hour at 4 °C with gentle rocking ...
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bioRxiv - Cell Biology 2021Quote: ... and cutting of the GST tag from purified proteins using PreScission protease (GE Healthcare) in 150 mM NaCl ...
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bioRxiv - Cell Biology 2019Quote: ... cell lysates of these complexes were firstly treated with GST agarose beads (GE Healthcare). And then the protein-bound beads were transferred into gravity columns and treated with 3C protease overnight at 4 °C ...
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bioRxiv - Plant Biology 2019Quote: ... Peptides were purified via GST affinity chromatography by FPLC (Äkta Prime Plus, GE Healthcare). The GST tag was proteolytically cleaved by TEV-protease digestion ...
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bioRxiv - Cell Biology 2019Quote: ... The chip was prepared by covalent coupling of an anti-GST antibody (GE Healthcare) as capture molecule to the dextran surface of both flow cells of a CM5 Chip (GE Heathcare ...
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Receptor-like role for PQLC2 amino acid transporter in the lysosomal sensing of cationic amino acidsbioRxiv - Cell Biology 2020Quote: ... and the GST-WDR41 fusion was generated in pGEX-6T-1 (GE Life Sciences). These new plasmids were generated by Gibson Assembly or Q5 Site-Directed Mutagenesis according to manufacturer instructions (New England Biolabs) ...
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bioRxiv - Plant Biology 2021Quote: ... using the GST gene fusion system for heterologous expression and affinity purification (GE Healthcare). DNA templates for the cystatins were synthesized as g-blocks (IDT ...
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bioRxiv - Biochemistry 2020Quote: ... MBP-tagged or GST-tagged proteins immobilized on Amylose beads (GE Healthcare Life Science) or Glutathione Sepharose (GE Healthcare Life Science ...