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Citations for GE Life Sciences :
501 - 550 of 3680 citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The crude extract was applied to 1ml Glutathione Sepharose beads (GE Healthcare) previously equilibrated in Buffer A (PBS ...
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bioRxiv - Cell Biology 2019Quote: ... Protein was purified using glutathione sepharose 4B beads (GE healthcare life science). 20 mM concentration of reduced glutathione was used for protein elution ...
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bioRxiv - Cell Biology 2019Quote: ... Clarified cell lysate was loaded onto Glutathione (GSH) Sepharose beads (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was then loaded on Glutathione Sepharose 4B column (GE Healthcare) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Equal protein amounts were incubated with Glutathione Sepharose® 4B (GE Healthcare) charged with GST fusion proteins overnight at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded onto a 5-mL glutathione column (GE Healthcare), followed by washing with 20 column volumes of buffer G plus 300 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... the supernatants were incubated with 2 mL glutathione-Sepharose beads (GE Healthcare) at 4°C for 3 hrs ...
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bioRxiv - Biophysics 2022Quote: ... Lysates containing FAT were incubated with Glutathione Sepharose 4B beads (GE healthcare). The beads were thoroughly washed with 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Developmental Biology 2022Quote: ... from which soluble forms were purified on glutathione-Sepharose beads (GE Healthcare). Recombinant C-terminally HA tagged Su(H ...
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bioRxiv - Developmental Biology 2021Quote: ... the supernatant was incubated with 5 ml Glutathione Sepharose 4B (GE Healthcare) for 1 h at 4 °C in batch mode under gentle rotation ...
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bioRxiv - Molecular Biology 2020Quote: ... Resultant supernatant was incubated with 100μL of Glutathione Sepharose beads (GE healthcare) overnight with constant mixing at 4°C ...
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Control of cortical cytoskeleton-membrane interaction by RhoA regulates peripheral nerve myelinationbioRxiv - Neuroscience 2021Quote: ... for Rho proteins) that was immobilized on Glutathione Sepharose beads (GE Healthcare). Bait-couple beads were incubated with lysates incubated with overnight at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... gravity flow columns containing 0.2 mL glutathione Sepharose 4B resin (GE Healthcare), and incubated for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... The solution was added to glutathione Sepharose 4B (GE Healthcare, Chicago, IL) and incubated 4 h at 4°C under agitation ...
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bioRxiv - Biochemistry 2020Quote: ... the supernatant was purified by Glutathione Sepharose 4B column chromatography (GE Healthcare) followed by digestion with PreScission protease (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... and purified using Glutathione Sepharose 4B beads (GE Healthcare, #17-0756-01). The purified protein was injected into rabbits and the antisera were collected (Cocalico).
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bioRxiv - Biophysics 2019Quote: ... the supernatant was loaded onto a Glutathione Sepharose 4B resin (GE Healthcare) column ...
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bioRxiv - Cell Biology 2021Quote: ... The lysates were added directly on glutathione sepharose 4B (GE Healthcare, #17075601) for 2h ...
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bioRxiv - Biochemistry 2021Quote: ... Cleared lysate was applied to glutathione sepharose resin (GE Healthcare Life Sciences), incubated for 1h with the resin ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was incubated with 30 µl Glutathione Sepharose beads (GE Healthcare) at 4°C for 4 hours ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was purified over Glutathione Sepharose 4 Fast Flow (GE Healthcare) and eluted from the beads using 50 mM Tris-HCl pH 8 ...
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bioRxiv - Microbiology 2022Quote: Protein purification was achieved using a glutathione Sepharose 4B column (GE Healthcare) following the recommended protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the supernatant was incubated with Glutathione-Sepharose 4B beads (GE Healthcare) for 2 hrs at 4 °C under gentle rotation and then centrifuged at 4,000 x g for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was applied to Glutathione Sepharose™ 4B beads (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... and the supernatants were incubated with glutathione-Sepharose 4B beads (GE Healthcare). The beads were collected ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were purified by using glutathione Sepharose beads (GE Healthcare, Waukesha, WI), and subsequently sent to Eurogentec (Seraing ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant was passed through a 5 ml Glutathione Sepharose 4B (GE Healthcare) equilibrated with buffer A2 using an AKTA system (GE Healthcare) ...
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bioRxiv - Cell Biology 2023Quote: ... The clear supernatant fraction was incubated with glutathione Sepharose bead (GE healthcare). GST fusion proteins on beads were incubated with recombinant proteins at 25 °C for 2 hours or 4 °C overnight in reaction buffer (25 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... Supernatants were incubated with glutathione Sepharose (GSH) fast flow beads (GE Healthcare) for GST-tagged proteins or nickel– nitriloacetic acid (Ni-NTA ...
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bioRxiv - Cell Biology 2024Quote: ... Bound proteins were then purified via binding to glutathione Sepharose (GE Healthcare) and washed ...
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bioRxiv - Microbiology 2021Quote: ... Fluorescent CFUs were determined by imaging with a Typhoon FLA7000 fluorescent scanner (GE Healthcare).
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bioRxiv - Neuroscience 2023Quote: ... was used and the fluorescent signal acquired with a Typhoon fluorescent scanner (GE Healthcare). Band intensities were quantified using ImageQuant software (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was clarified by centrifugation (15000 rpm for 1 h at 4 °C) and incubated with glutathione Sepharose 4B (GE Healthcare) for 4 h at 4 °C ...
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bioRxiv - Biochemistry 2019Quote: ... Bacterial lysates were collected as described above and the lysates were incubated with 1 ml Glutathione Sepharose 4B (GE Healthcare, UK) at 4°C for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: GST or GST-tagged β’-COP (residues 1-604) or Glo3 constructs (GAP domain, BoCCS region, GRM region) were immobilized on glutathione sepharose resin (GE Healthcare) for one hour on ice ...
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bioRxiv - Microbiology 2022Quote: IVTT reaction mixtures (100 µl) were incubated at 4 °C for 1 h with a 50% slurry of PBS/Glutathione-Sepharose 4B (GE Healthcare) with gentle mixing ...
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bioRxiv - Plant Biology 2022Quote: ... coli strain BL21 with protein induction when OD600 reached 0.5 with 1 mM IPTG (isopropyl b-D-1-Thiogalactopyranoside) for 4 h at 37°C and then purified over a glutathione Sepharose 4B column (GE Healthcare). The eluted samples were then resolved by SDS-PAGE and stained by Coomassie brilliant blue R-250 to evaluate purity ...
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bioRxiv - Immunology 2023Quote: ... The proteins were induced in Escherichia coli BL21 (Gold) cells with 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) for 5 hour and purified by glutathione sepharose (GE healthcare) followed by on-column TEV cleavage and finally by size-exclusion chromatography on Hiload 16/60 Superdex 75 coloumn (GE healthcare).
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bioRxiv - Cell Biology 2019Quote: ... coli GST sequence on the pGEX-2T plasmid (GE Healthcare, Freiburg, Germany) using BamHI and EcoRI restriction sites ...
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bioRxiv - Cell Biology 2019Quote: ... except that the column was connected to a GST-Trap (GE Healthcare) for online removal of GST and the GST-3C protease.
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded onto a 4-mL GST column (GE Healthcare) equilibrated in PBS containing 1 mM EDTA ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysates were incubated with 250 μL of GST Sepharose 4B (GE Healthcare) for 3 hours at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... coli GST sequence on the pGEX-2T plasmid (GE Healthcare, Freiburg, Germany) using BamHI and EcoRI restriction sites ...
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bioRxiv - Cell Biology 2020Quote: ... The soluble fraction was bound to the GST Sepharose 4B (GE Healthcare) for 90 to 120 min at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified supernatant was purified on GST Sepharose 4B resin (GE healthcare) and then eluted in the lysis buffer with 25mM glutathione ...
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bioRxiv - Developmental Biology 2022Quote: ... GST fusion proteins were affinity purified over Gluthione Sepharose 4B (GE Healthcare). After extensive washing ...
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bioRxiv - Cell Biology 2022Quote: ... GST-Lc3b was constructed by cloning Lc3b into the BamHI and SalI sites of pGEX-4T-1 (GE Healthcare, IL, USA). The pac sequence was amplified from pBApo-EF1α Pur and cloned into pBApo-EF1α Neo ...
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bioRxiv - Immunology 2019Quote: ... ERV-K envelope protein was cloned out of the sequencing vector and ligated into pGEX-6p-1 N’ GST-tag expression vector (GE Healthcare) between EcoRI and NotI sites using the following primers:
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bioRxiv - Cell Biology 2021Quote: ... and GST-SV40-NLS were cloned by in-frame insertion of the corresponding NFAT5 fragments and SV40-NLS into the C-terminus of GST of pGEX-4T-1 (GE Healthcare), respectively ...
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bioRxiv - Genomics 2021Quote: ... and AsNRDE-3 amino acids 45-246 (HQ611973) were fused in frame with GST using the Bam H1 site of pGEX-6P-1 (GE Healthcare), the proteins were expressed in E ...