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Citations for GE Life Sciences :
351 - 400 of 5991 citations for Glioma pathogenesis related protein 1 GliPR1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... All Parkin constructs were further purified over Anion exchange column Hi-trap Q HP (GE Healthcare) followed by Hiload 16/600 Superdex 200pg column (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... supernatants filtered through 0.45 µm syringe filter and loaded onto His Trap (5 mL, GE Healthcare) equilibrated with 5% HisB buffer (100% HisB ...
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bioRxiv - Biophysics 2019Quote: ... 1 mM DTT at pH 7.0) while incubating with PreScission protease (protease to protein ratio 1:2000) (GE Healthcare) overnight ...
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bioRxiv - Neuroscience 2020Quote: ... monkey (XP_005545315.1; 121-760aa) and human (NP_001121620.1; 120-760aa) TfR1 in 0.1% BSA in HBS-EP+ buffer (GE Healthcare) was captured at flow rate 10 μl/min ...
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bioRxiv - Cell Biology 2019Quote: GST-tagged mammalian ATG8 fusion proteins were cloned into pGEX-4T-1 (GE Healthcare) and expressed in Escherichia coli BL21 (DE3 ...
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bioRxiv - Biochemistry 2019Quote: ... Protein expression was induced by adding (isopropyl β-D-1-thiogalactopyranoside, IPTG; GE Healthcare) to a final concentration of 0.3 mM ...
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bioRxiv - Microbiology 2021Quote: ... the protein was concentrated to 1 mg/ml using a vivaspin 20 (GE Healthcare) and store at −80 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Recombinant proteins were immobilized on a HiTrap Talon crude 1 ml column (GE Healthcare), equilibrated with buffer A (50□mM NaH2PO4 (pH 8) ...
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bioRxiv - Plant Biology 2023Quote: ... coli or insect cells as fusion proteins subcloned into pGEX-6P-1(GE Healthcare) or te pFastBac-1 vector (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were immobilized on a HiTrap Talon crude 1 mL column (GE Healthcare), equilibrated with buffer A (50 mM NaH2PO4 (pH 8) ...
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bioRxiv - Plant Biology 2019Quote: ... Cleaved GFP-8His and the His-tagged protease were removed using a HisTrap nickel column (GE Healthcare), and the untagged target AtGAAP3 was harvested from the flow-through ...
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bioRxiv - Microbiology 2019Quote: ... Clarified lysate was loaded onto a 5 mL His-Trap Ni-NTA column (GE Healthcare Life Sciences) using an Akta Pure (GE Healthcare Life Sciences) ...
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bioRxiv - Molecular Biology 2020Quote: ... The cell-free lysate was applied into a 5 ml Hi Trap Chelating HP column (GE Healthcare) equilibrated in start buffer ...
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bioRxiv - Microbiology 2020Quote: ... Purification was done using 5 mL pre-packed Ni-sepharose AKTA Hi-TRAP column (GE Healthcare, USA) equilibrated (100 mM NaH2PO4 ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 µl of equilibrated in lysis buffer His Mag Sepharose beads Ni (GE28-9799-17, GE Healthcare) was added to each sample and kept on a roller overnight ...
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bioRxiv - Cell Biology 2022Quote: ... the 6x His-MBP fusion was further purified on a Superdex 200 16/60 column (GE Healthcare), in 50 mM Tris pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... The recombinant 3CLpro were then purified using HIS Gravitrap Ni-NTA affinity columns (GE Healthcare, Chicago, IL), following the standard protocol (Kim et al. ...
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bioRxiv - Biochemistry 2021Quote: HR1 with C-terminal poly-his tag was immobilized onto a NTA sensor chip (from GE Healthcare) to around 500 RU using a Biacore SPR 8K (GE Healthcare ...
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bioRxiv - Genetics 2019Quote: ... This diluted sample was then loaded onto a 5 mL Hi-Trap Q HP column (GE Healthcare) and eluted over a linear gradient of 150 mM to 1000 mM NaCl with the protein eluting at 225 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... and the supernatant was subjected to affinity purification with a His-Trap HP 5 mL (GE Healthcare) column ...
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bioRxiv - Biochemistry 2023Quote: ... gel filtration chromatography with Superdex-75 SEC column (Hi-Load 16/600, Prep Grade, GE Healthcare, USA) and MALDI analysis were used to determine the molecular mass of PrASNase [23].
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bioRxiv - Plant Biology 2023Quote: ... Each of the His-tag-fused LssaCA muteins was bound to Ni Sepharose (GE Healthcare, Uppsala, Sweden), then the GST-NP protein or the GST control protein was added to the beads ...
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bioRxiv - Plant Biology 2023Quote: ... GST-SnRK2.6 and His-MBP-SIZ1-C were mixed separately with high-affinity GST resins (GE Healthcare) in a pull-down buffer containing 50 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Microbiology 2023Quote: ... was then purified by Ni-NTA using a His-TRAP FF 5-ml column (GE Healthcare®). Collected elutions were dialyzed into 10 mM HEPES ...
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bioRxiv - Biophysics 2024Quote: ... ISCU2 and FXN were subjected to Ni-NTA affinity chromatography (His-Trap 5ml FF crude; GE Healthcare). The column was washed with 10 CV IMAC wash buffer ...
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Undruggable oncoproteins cMyc and NMyc bind to mediator of transcription with superior high affinitybioRxiv - Biochemistry 2024Quote: ... The supernatant was loaded on the His trap HP 5 mL column packed with Nickel (GE Healthcare). FPLC was used for elution of the GST-His6x-TEV-KIX protein with 300 mM imidazole in 50 mM Tris-HCl buffer (150 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: Human ileal enteroids were transduced with GIPZ lentiviral shRNA kit for human Coronin 1a (GE Healthcare Dharmacon) based on protocol adapted from Heijmans et al.17 ...
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bioRxiv - Bioengineering 2021Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated from fresh human peripheral blood using Ficoll (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Immunology 2020Quote: ... Human peripheral blood mononuclear cells (PBMC) were separated from healthy human blood samples using Ficoll-Paque (GE healthcare) or Lymphoprep (Stem Cell ...
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bioRxiv - Cancer Biology 2023Quote: ... human ERBB4 siRNA pool and human ERBB2 siRNA pool were purchased (Dharmacon GE Healthcare, now Horizon Discovery Ltd.). miRNA mimics ...
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bioRxiv - Immunology 2021Quote: ... the mAb was purified using a 1 mL HiTrap Protein A HP column (GE Healthcare). The media was diluted with an equal volume of Protein A IgG Binding Buffer (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM DTT) and protein was eluted by ca 40 units PreScission protease (GE Healthcare) for 4 hours at 4°C under rotation ...
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bioRxiv - Cell Biology 2021Quote: ... and then for another 1 h following the addition of protein-G Sepharose (GE Healthcare). Protein bound to antibody beads was washed five times with lysis buffer at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... The ITC protein sample p52 (1-398) went through desalting column (HiTrap desalting, GE Healthcare) to freshly made ITC buffer containing 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Microbiology 2020Quote: ... using multiple runs over a 1 ml Protein-A HiTrap column (GE Healthcare Life Sciences) on an ÄKTA FPLC (GE Healthcare Life Sciences) ...
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bioRxiv - Neuroscience 2023Quote: ... and Synaptotagmin-1 protein was subjected to anion exchange chromatography (MonoS, GE Healthcare, Chicago, IL) to remove nucleotide contaminants ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by 1 h of incubation with protein A–Sepharose CL-4B beads (GE Healthcare). Immunoprecipitates were washed three times in lysis buffer ...
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bioRxiv - Immunology 2023Quote: ... The protein solution was loaded on to a 1 ml Mono S column (GE Healthcare), eluted with a 20-bed volume linear gradient from 0 to 500 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... RAS:Affimer immunoprecipitation was as for RAS:RAF immunoprecipitation except His Mag Sepharose™ Ni2+-NTA beads (GE healthcare®) were used and incubated with 20 µg of Affimer K3 for 1 h with agitation ...
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bioRxiv - Biochemistry 2020Quote: ... Ni Sepharose High Performance (His Trap HP) and Superose 6 10/300 column were purchased from GE Healthcare.
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bioRxiv - Biochemistry 2021Quote: ... followed by gel filtration chromatography using Hi-Load 16/600 superdex 200 pg and 75 pg (GE Healthcare) on AKTA pure and AKTA prime systems ...
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bioRxiv - Microbiology 2020Quote: ... Untagged His-GmPUB13 U-box and Avr1d were loaded on a superdex 200 gel-filtration column (GE Healthcare) and eluted in superdex buffer (20 mM Tris [pH 8.0] ...
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bioRxiv - Biophysics 2021Quote: ... and the final product was obtained by running the solution through an 5 mL Hi-Trap (GE Healthcare) desalting column (Figure S1).
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bioRxiv - Biochemistry 2022Quote: ... The clarified supernatant was then passed over a Ni-NTA His- Trap FF 5 ml column (GE Healthcare) which was pre-equilibrated with loading buffer composed of 25 mM HEPES pH 7.0 ...
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bioRxiv - Biophysics 2022Quote: ... The clarified supernatant was then passed over a Ni-NTA His-Trap FF 5 ml column (GE Healthcare) which was pre-equilibrated with loading buffer composed of 25 mM HEPES pH 7.0 ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was then applied onto a Hi Trap affinity (5 ml) (Ni2+) column (GE Healthcare Life Sciences) The column was washed with 10 column volumes of buffer A ...
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bioRxiv - Microbiology 2020Quote: ... Supernatant was incubated with His Mag Sepharose beads (equilibrated with lysis buffer according to manufacturer’s instructions; GE Healthcare) for 30 min at 4 °C rotating end-over-end ...
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bioRxiv - Plant Biology 2021Quote: ... The 6×His-tagged AVR-PikD (ligand) was immobilised on the Series S Sensor Chip NTA (GE Healthcare) and the HMA constructs (analytes ...
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bioRxiv - Genetics 2019Quote: The clarified lysate containing mZCWPW1 was then loaded onto a 5 mL His-Trap HP column (GE Healthcare) using NGCTM chromatography system (BioRad) ...
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bioRxiv - Microbiology 2021Quote: ... The clarified lysate was first affinity-purified on an ÄKTA Purifier using His-Trap HP columns (GE Healthcare) and eluted with elution buffer (50 mM HEPES pH 7.4 ...