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Citations for GE Life Sciences :
51 - 100 of 106 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... The mix was then run on a Superdex 200 10/300 column (GE Healthcare Life Sciences) equilibrated in 50 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... Signals were amplified by Chemi-Reagent Mix and visualized using an imageQuant LAS4000 mini (GE Healthcare) and quantified by ImageStudioLite (LICOR) ...
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bioRxiv - Molecular Biology 2023Quote: ... The input was collected from the mix and 50μl of equilibrated glutathione sepharose 4B (GE healthcare) were added to the reaction mix ...
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bioRxiv - Biochemistry 2021Quote: SARM1-dN tagged with strep-tag II and flag-tag was immunoprecipitated with StrepTactin resin (GE healthcare), washed with buffer W (100 mM Tris-HCl pH8.0 ...
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bioRxiv - Microbiology 2021Quote: ... Individual images were also obtained with a 100x objective on a DeltaVision II deconvolution microscope (Applied Precision Inc. ...
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bioRxiv - Zoology 2020Quote: ... and 3500 V/2 mA/5 W for 6 h using a Multiphor II system (GE Healthcare). The IEF strip was then equilibrated for 15 min in 5 ml equilibration stock solution (6 M urea ...
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bioRxiv - Molecular Biology 2019Quote: ... was labeled with dCTP α-32P using Amersham Rediprime II DNA Labelling kit (GE Healthcare, Buckinghamshire, England) and used for hybridization at 42 °C ...
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bioRxiv - Biochemistry 2021Quote: ... IEF was then performed on a Multiphor II Electrophoresis System with ImmobilineDryStrip Kit (Amersham Biosciences, Ge Healthcare), in 4 consecutive steps ...
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bioRxiv - Molecular Biology 2023Quote: ... using the Amersham Rediprime II Random Prime Labeling System (RPN 1633; GE Healthcare, Chalfont St Giles, UK). Membranes were hybridized (250 mM Na2HPO4 ...
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bioRxiv - Microbiology 2022Quote: ... 500 μg of a 1:1 mix of hydrophobic and hydrophilic beads (GE Healthcare 65152105050250 and 45152105050250) was added to each sample ...
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bioRxiv - Cell Biology 2023Quote: ... a chemiluminescence reagent mix was added and measurements were performed using an Amersham Imager 600 (GE Healthcare) and quantified with ImageJ.
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bioRxiv - Biochemistry 2023Quote: MALS experiments were done on a Wyatt HELEOS II scattering detector coupled to an ÄKTA FPLC (GE Healthcare) with a Superose 6 Increase 10/300 column using HBS as the sample and running buffer ...
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bioRxiv - Plant Biology 2021Quote: ... The gels were scanned at 300 dpi to obtained digital images (TIFF files) using imagescanner (Epson Expression 11000XL) and spots detection was performed with Image Master 2D Platinum Software (GE Healthcare). The spots were matched and detected by choosing a gel as the reference gel semi-automatically (manual correction and with default spot analysis setting in the software) ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were then incubated with Chemi Reagent Mix and developed in ImageQuant LAS 500 imaging system (GE Healthcare). For protein level quantification ...
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bioRxiv - Microbiology 2020Quote: ... The assembly mix was concentrated at 350 × g using Vivaspin filters (10 kDa molecular weight cutoff; GE Healthcare) and subjected to another round of size-exclusion chromatography using the same column and buffer to remove unassembled components ...
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bioRxiv - Molecular Biology 2020Quote: ... The assembled transcribing Pol II complex was then applied onto a Superdex 200 Increase 3.2/300 column (GE Healthcare) equilibrated with the SEC150 buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... The general purification strategy was as follows: (i) Nickel (II) affinity chromatography (Ni Sepharose 6 Fast Flow, GE Healthcare) in 25 mM Tris (pH 8.0) ...
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bioRxiv - Molecular Biology 2021Quote: ... and analyzed (normalization, spot matching, expression analyses, and statistics) using Image Master 2-D Platinum 6 image analysis software (GE Healthcare, USA). Spots on the gel were identified and each spot was assigned a spot quantity (q) ...
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bioRxiv - Biophysics 2023Quote: ... The cleavage mix was then passed through a sulphopropyl sepharose column (HiPrep SP FF 16/10 20ml, GE HealthCare) and washed with buffer C ...
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bioRxiv - Genomics 2022Quote: ... Dual X-ray absorptiometry (DXA) was performed on the femurs and lumbar vertebrae using the Lunar Piximus II (GE Healthcare) as described previously by Beamer et al57 ...
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bioRxiv - Biochemistry 2021Quote: ... The excess Cu(II) was eliminated by passing the protein through a PD MidiTrap G-25 desalting column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 and 150 mM NaCl.
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bioRxiv - Immunology 2022Quote: ... HLA class-I and -II-associated peptides were isolated using the pan-HLA class I-specific mAb W6/32 and the pan-HLA class II-specific mAb TÜ39 (both in house mouse monoclonal) covalently linked to CNBr-activated Sepharose (GE Healthcare). HLA-peptide complexes were eluted by repeated addition of 0.2% TFA (trifluoroacetic acid ...
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bioRxiv - Microbiology 2022Quote: ... Transfer was performed in an XCell II Blot Module in NuPAGE Transfer Buffer (Thermo Fischer) onto a Hybond P 0.45 PVDF membrane (GE Healthcare). After blocking in 5% BSA or milk powder (Carl Roth ...
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bioRxiv - Biochemistry 2020Quote: Size exclusion chromatography was performed on a quaternary Agilent 1290 Infinity II UPLC system equipped with a SEC column (Superdex Peptide 3.2/300, GE healthcare). 40 μg of the eight protein mixture was loaded and separated at a flow rate of 0.05 ml/min with running buffer (30%ACN ...
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bioRxiv - Microbiology 2022Quote: ... and the obtained supernatant was purified using 0.22 μm filter and passed over a Strep-Tactin II column (GE Healthcare) to collect secreted TraC ...
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bioRxiv - Microbiology 2023Quote: ... Transfer was performed in an XCell II Blot Module in NuPAGE Transfer Buffer (Thermo Fischer) onto a Hybond P 0.45 PVDF membrane (GE Healthcare). After blocking in 5% BSA or milk powder (Carl Roth ...
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bioRxiv - Biophysics 2023Quote: ... Pol II (with a protein A tag in the Rpb3 subunit) was purified by an IgG affinity column (GE Healthcare), followed by Hi-Trap Heparin (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... MK2 was subsequently removed from the reaction mix by another round of SEC (Superdex 75, 10/300 GL, GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... The chromatin was then precleared overnight at 4°C with vertical rotation at 15rpm (a total of 5 reactions per condition in 15ml tubes) with 500µls of a mix of Protein G and A SepharoseTM 4 Fast flow (GE Healthcare) beads and then used for immunoprecipitation.
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bioRxiv - Molecular Biology 2021Quote: ... and immunoprecipitated again in a single reaction with 100µls of a mix of Protein G and A SepharoseTM 4 Fast flow (GE Healthcare) pre-conjugated with histone H3 and histone H2B antibodies in order to specifically enrich for chromatin-associated dCas9 complexes and eliminate from the purification unspecific non-chromatin associated dCas9 complexes ...
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bioRxiv - Microbiology 2020Quote: ... the assembly mix was sterile filtered (0.22 μm) and applied to a Superose 6 increase 10/300 GL column (GE Healthcare) to remove unassembled components ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Samples were homogenised by bead beating at 4°C in 500 μl Tris-HCl lysis buffer (100 mM, pH 6.8) containing 1% Protease Inhibitor Mix (GE Healthcare). A full and detailed description of the protocol for 2-dimensional gel electrophoresis (2-DE ...
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bioRxiv - Molecular Biology 2020Quote: ... The gel was stained in Sybr Green II and scanned on a Typhoon 9410 Variable Mode Imager (GE Healthcare, Piscataway, NJ).
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bioRxiv - Molecular Biology 2019Quote: ... and ii) the final step of the purification involved gel filtration through a Superdex 200 Increase 10/300 GL (GE Healthcare) column in storage buffer (50 mM Tris-Cl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: SEC-MALS experiments were carried out with a DAWN HELEOS II detector (Wyatt Technology) with an in-line size exclusion Superdex 200 (10/300 GL) column (GE Healthcare) balanced in 10 mM phosphate ...
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bioRxiv - Plant Biology 2021Quote: ... Eluted proteins were copper saturated with Cu(II)SO4 and applied to a 120 mL Superdex 75/16 column (GE Healthcare) to remove the excess of copper ...
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bioRxiv - Immunology 2022Quote: ... After equilibration of the IPG strips in reduction buffer and alkylation buffer proteins were separated according to their molecular weight in the second dimension using a gradient gel (8%-15%) in an Ettan DALT II system (GE Healthcare). The gels were then scanned at the different wavelengths using a Typhoon 9400 Laser Scanner (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... size exclusion chromatography (SEC) coupled to MALS analysis was performed on MiniDAWN TREOS II coupled to S200 10/300 increase column (GE Healthcare) mounted on FPLC system (AKTA purifier) ...
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bioRxiv - Cell Biology 2023Quote: SEC-MALS experiments were carried out on DAWN HELEOS-II (Wyatt Technology Corporation) coupled with a Shimadzu liquid chromatography system equipped with a Superdex 200 10/300 GL (GE Healthcare) gel-filtration column ...
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bioRxiv - Cell Biology 2023Quote: ... actin Ac-74 or Pol II S5P) overnight at +4°C and immune-complexes were collected with 50 μl of protein A sepharose (GE Healthcare) at +4°C for two hours on rotation ...
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bioRxiv - Biochemistry 2019Quote: ... and allowed to mix at room temperature for 30 minutes before injection on to a Superdex 200 Increase 10/300 column (GE Healthcare) equilibrated in 10 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... A 1:1 combination mix of two of magnetic carboxylate beads was used (Sera-Mag Speed beads, #45152105050250, #65152105050250, GE Healthcare). Beads were prepared fresh by rinsing with water three times prior to use at a stock concentration of 20 μg/μL ...
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bioRxiv - Microbiology 2021Quote: ... or an in vitro reconstituted NS1-HDL mix were analyzed by size exclusion chromatography on a Superdex 200 10/300 column (GE healthcare). Protein standards from Bio-Rad were used to interprete elution profiles ...
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bioRxiv - Biochemistry 2024Quote: ... 2 µL of a 1:1 mix of two types of commercially available carboxylate beads (Sera-Mag Speed beads, 45152105050250, 65152105050250, GE Healthcare), and 60 µL ethanol ...
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bioRxiv - Evolutionary Biology 2019Quote: ... gene specific fragments that were used for VIGS (primer pairs listed in Table S1) were radiolabeled with [α-32P] dCTP (Perkin-Elmer) using the Rediprime II DNA Labeling System (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... was combined with 250 μg carboxylated paramagnetic beads (1:1 mix of hydrophiclic and hydrophobic Sera-Mag SpeedBead Carboxylate-Modified, GE Life Sciences) and 750 μl ethanol ...
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bioRxiv - Biochemistry 2022Quote: ... The precipitated pellet was resuspended in Buffer A and the solution subjected to Ni(II) immobilized affinity chromatography using a 5 mL HisTrap FF column (GE Healthcare Life Sciences) with a gradient from 100% buffer A to 100% buffer B (25 mM Tris-HCl ...
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bioRxiv - Bioengineering 2020Quote: ... MRI scan acquired using a Dixon imaging sequence (Table II) acquired between two and four weeks before surgery on a clinical 3T MRI (Discovery MR750, GE Healthcare, Waukesha, WI). Regions of interest (ROIs ...
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bioRxiv - Immunology 2020Quote: HEK293T cells were grown at 37°C to approximately 80% confluence in 10 cm tissue culture dishes using DMEM with 5% Hyclone Fetal Clone II (GE Healthcare Lifesciences, Logan, UT) and 1X antibiotic and antimycotic (Gibco ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Probes were labled with 32P-dCTP (Hartmann Analytic, Braunschweig) by the use of the Rediprime II DNA Labeling Kit (GE Healthcare Life Science, Little Chalfont) according to the manufacturer’s manual ...