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Citations for GE Life Sciences :
301 - 350 of 4693 citations for Fatty acid binding protein FABP1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Harvested supernatants were applied to a Hi-Trap Nickel excel columns (GE Healthcare) using an Akta pure (GE Healthcare) ...
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bioRxiv - Immunology 2023Quote: ... Nbs from PE were purified using IMAC Hi-Trap columns (GE Healthcare, 17092003) following manufacturer’s instructions and eluted using 300 nM Imidazole ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was applied to a 5 ml His-TRAP column (GE Healthcare) and eluted with a gradient of 20 mM to 500 mM imidazole ...
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bioRxiv - Microbiology 2023Quote: ... The F341_RBP was purified using Ni2+- NTA-affinity chromatography (His GraviTrap, GE Healthcare) according to manufacturer’s instructions and exchanged into PBS buffer using 10 kDA Amicon® Ultra-15 Centrifugal filter units ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was loaded onto a nickel-chelated Hi-trap column (GE Healthcare) and eluted with a linear gradient of 25-500 mM imidazole in 20 mM HEPES (pH 7.5) ...
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bioRxiv - Molecular Biology 2024Quote: ... HIS-p300 was further purified on a Q sepharose column (HiTrap, GE Healthcare) in BC buffer and eluted between 100-600 mM NaCl ...
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bioRxiv - Microbiology 2024Quote: ... The NRP2-his was purified using a HiTrap IMAC FF Ni2+ (GE Healthcare) affinity column and Superdex 75 10/300 size exclusion chromatography (GE Healthcare ...
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bioRxiv - Cancer Biology 2019Quote: ... Primary antibodies were detected by binding goat anti-rabbit IgG-HRP (NA934, GE Healthcare), goat anti-mouse IgG-HRP (NA931 ...
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bioRxiv - Biochemistry 2020Quote: Binding studies were performed at 25°C using a MicroCal iTC200 instrument (GE Healthcare). ANT(9 ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibody binding was followed by anti-mouse (GE Healthcare, catalog # NXA931V, lot # 17041890) or anti-rabbit IgG (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: SPR binding experiments were collected on a Biacore T200 or S200 Instrument (GE Healthcare). Neutravidin (Pierce ...
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bioRxiv - Immunology 2021Quote: ... Recombinant CEACAM6 protein consisting of the CEACAM6 extracellular domain fused to the constant region of human IgG were produced in HEK-293 cells and purified via protein G columns (GE Healthcare, Munich, Germany) as described previously (71).
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bioRxiv - Molecular Biology 2021Quote: ... using a Hi-Load 16/600 Superdex 200 prep grade column (GE Healthcare Bioscience) equilibrated with 20 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... The cell lysate was loaded to a His-trap column (GE Healthcare, IL, USA), washed with phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2020Quote: ... and supernatants loaded onto 5 mL HP HIS-TRAP columns (GE Healthcare Life Sciences) equilibrated in buffer A (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Biophysics 2022Quote: ... The clarified lysate was loaded onto a 5mL His Trap HP column (GE Healthcare) pre-equilibrated in buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.4 and applied to a 5 mL Hi-Trap NHS column (GE Healthcare), which contained 20 mg of immobilised E141A H164A OmCI protein ...
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bioRxiv - Cell Biology 2020Quote: ... the elution was further purified by Hi-trap Q anion-exchange chromatography (GE Healthcare). Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3 ...
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bioRxiv - Cell Biology 2019Quote: ... the elution was further purified by Hi-trap Q anion-exchange chromatography (GE Healthcare). Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3 ...
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bioRxiv - Microbiology 2020Quote: ... SARS2-CoV-2 RBD-His was immobilized to a CM5 sensor chip (GE Healthcare). Serial dilutions of purified recombinant Nanosota-1 drugs were injected at different concentrations ...
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bioRxiv - Microbiology 2021Quote: ... The crude extract was applied onto a nickel chelation column (His GraviTrap, GE Healthcare), and TasA28-261-His was eluted according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Subsequently hCD14-his was purified on a 5 mL HisTrap Excel column (GE Healthcare) by employing ÄKTA Pure system (GE Healthcare) ...
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bioRxiv - Biophysics 2023Quote: ... and applied to a Hi Trap SP HP column (GE Healthcare #17-1151-01) equilibrated with dCas9-Cys dialysis buffer ...
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bioRxiv - Biophysics 2023Quote: ... and applied to a Hi Trap SP HP column (GE Healthcare #17-1151-01) equilibrated with dCas9 dialysis buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were applied to Nickel-Nitrilotriacetic (Ni-NTA) His-Trap FF column (GE Healthcare) and 6xHis-tagged-mCherry-p62 was eluted via a stepwise imidazole gradient (50 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... GgUox was purified using a 5 mL His-trap Ni-NTA column (GE Healthcare) equilibrated in 50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Biophysics 2023Quote: ... loaded onto a Hi-Trap heparin affinity column (2×5 ml, GE Life Sciences), and eluted with a salt gradient (200–800 mM NaCl) ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was passed through a His Prep FF 16/10 column (GE Healthcare) and washed with 5 column volumes of buffer ...
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bioRxiv - Biophysics 2023Quote: ... Subsequent removal of DTT was achieved with a Hi-Trap desalting column (GE Healthcare) equilibrated in buffer A without β-mercaptoethanol ...
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bioRxiv - Biochemistry 2024Quote: ... ZapD and His-Smt3 were separated by chromatography in 5-ml HisTrap (GE Healthcare), then the protein was eluted in a 5-ml HiTrap Desalting column (Healtcare ...
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bioRxiv - Cell Biology 2020Quote: ... Chemiluminescence signal from antibody binding was detected using ECL Select detection kit (GE healthcare, GERPN2235) with a ChemiDoc system (BioRad).
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bioRxiv - Biochemistry 2020Quote: ... The binding study was carried out at 25°C in PBS-P buffer (GE Healthcare). The heparin molecule or fondaparinux at different concentrations were flowed over the immobilized recombinant proteins at a flow rate of 30 μl/min with a contact time of 60 s and a dissociation time of 100 s ...
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bioRxiv - Biochemistry 2020Quote: Binding affinity of a repebody was determined through surface plasmon resonance (Biacore T200, GE Healthcare). Briefly ...
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bioRxiv - Microbiology 2021Quote: RBD binding to ACE2 was analysed on a Biacore T200 instrument (GE Healthcare Life Sciences) at 37°C and a flow rate of 30 μl/min ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... Primary antibody binding was followed by anti-mouse-IgG (GE Healthcare, catalog #NXA931V, lot #17193521) or anti-rabbit-IgG (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: Equilibrium binding experiments were performed at 25°C using a Biacore T200 instrument (GE Healthcare) using PBS + 0.005% (v/v ...
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bioRxiv - Microbiology 2023Quote: Antibody binding properties were analysed at 25 °C using a Biacore 8K instrument (GE Healthcare) with 10 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: SPR studies for studying binding kinetics were carried out using BIACORE T200 (GE Healthcare, USA) with Ni-NTA research grade sensor chip (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... Nickelnitrilotriacetic acid-agarose was from GE Healthcare. Deidentified ...
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bioRxiv - Molecular Biology 2021Quote: 1ug of soluble His-Rap1 was pre-cleared on Glutathione sepharose 4B beads (GE Healthcare) for 1h at 4C ...
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bioRxiv - Biochemistry 2019Quote: ... The His-tagged UBC13 and its variants were bound to HisTrap HP columns (GE Healthcare) and were eluted with 100mM-500mM Imidazole gradient ...
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bioRxiv - Bioengineering 2021Quote: ... and purified by his tag affinity to a nickel column (Histrap, GE healthcare, Chicago, Illinois) via fast protein liquid chromatography (FPLC) ...
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bioRxiv - Biochemistry 2020Quote: ... hydrophobic interaction chromatography using a Hi-Trap Butyl-Sepharose column (GE Healthcare, Cat. No. 28411001) with a flow rate of 1 ml/min and a gradient from 0.9 M (NH4)2SO4 in PBS to PBS over 5 CV was performed ...
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bioRxiv - Biochemistry 2020Quote: ... the soluble fraction was loaded onto a His Trap HP 5 mL column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was loaded onto a 1mL His-Trap FF column (GE Healthcare Life Sciences) and ZapB was eluted with an imidazole gradient (0 to 0.5M) ...
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bioRxiv - Microbiology 2020Quote: ... Concentrated samples were loaded onto Hi Load 16/600 Superdex 75 pg column (GE Healthcare) connected to the NGC Scout Chromatography system and running buffer composed of 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... The eluate was applied to a Hi-Load Superdex 200 16/60 column (GE Healthcare) in a buffer A ...
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bioRxiv - Microbiology 2020Quote: ... And further purified by gel filtration on a Hi Load Superdex 75 column (GE Healthcare). Peak fractions were concentrated to 10 mg/ml in 20 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged ZO-1-PDZ2 were purified using a HiTrap Chelating HP (GE Healthcare), where a renaturation gradient by exchange of denaturation buffer to a gel filtration buffer (Tris 50 mM (pH 7.5) ...
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bioRxiv - Microbiology 2020Quote: ... The KLKs were isolated via the 6 × His tag using nickel resin (GE Healthcare, Poland) according to the manufacturer’s protocol ...