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Citations for GE Life Sciences :
201 - 250 of 3149 citations for Estriol 2 4 16 17 D4 98% 95% Pure since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 0.2% Triton X-100) and incubated 1 hour at RT or 16 hours at 4°C with HRP-coupled streptavidin (RPN1231VS, GE Healthcare) diluted 1:20000 in BSA blocking buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions that contained BED were concentrated and further purified at 4 °C on a preparative Superdex 75 16/600 size exclusion column (GE Healthcare) equilibrated with 25 mM HEPES.Na pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions that contained the protein were concentrated and further purified at 4 °C on a preparative Superose 6 XK 16/70 size exclusion column (GE Healthcare) equilibrated with 25 mM HEPES.Na pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... the N-terminal His tag was cleaved by overnight dialysis with TEV protease at 4 °C and purified by size exclusion chromatography (SEC) (HiLoad Superdex-200 16/600 column, GE Healthcare) in CHIP storage buffer (50 mM HEPES ...
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bioRxiv - Biophysics 2024Quote: ... The peak elution fractions were pooled and concentrated to 2 mL using a Amicon Ultra 10K MWCO centrifugal filter at 4 °C and was loaded into a HiLoad Superdex 200 16/60 column (GE healthcare) equilibrated with 20 mM HEPES pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... on an AKTA Pure 25L protein purification system (GE Healthcare). The column was washed with 10 column volume (CV ...
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bioRxiv - Molecular Biology 2021Quote: ... and subsequently purified using an Äkta Pure system (GE Healthcare) as previously described (19) ...
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bioRxiv - Biophysics 2020Quote: ... using an ÄKTA pure chromatography system (GE Healthcare Life Sciences). Eluted fractions containing monomeric H57-scFV were concentrated with 10 kDa Amicon® Ultra-4 centrifugal filters (MERCK) ...
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bioRxiv - Biochemistry 2021Quote: ... at 5 mL/min by an AKTA Pure (GE Healthcare). The 5 mL HisTrap column was washed with 10 column volumes of wash buffer (2× GIBCO 14200-075 PBS ...
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bioRxiv - Bioengineering 2021Quote: ... in binding buffer on an Äkta Pure FPLC (GE healthcare) with sample pump ...
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bioRxiv - Biophysics 2022Quote: ... in an ÄKTA Pure purification system (GE Healthcare Life Sciences). The protein was eluted as 5 mL fractions using a linear gradient of lysis buffer from 0 to 1 M NaCl ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein was purified using an AKTA Pure HPLC (GE Healthcare) fitted with a HisTrap HP 5-ml column (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... and purified using an Äkta pure chromatography system (GE Healthcare). Following extensive wash with 12 column volumes (CV ...
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bioRxiv - Immunology 2021Quote: ... and the AKTA™ Pure system (GE Healthcare, Life Sciences) was used for purification ...
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bioRxiv - Molecular Biology 2020Quote: ... peptides were fractionated on an ÄKTA Pure system (GE Healthcare) using a Superdex Peptide 3.2/300 (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... connected to an ÄKTA pure FPLC system (both GE Healthcare). 250 µl fractions were collected and analysed on SDS-PAGE ...
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bioRxiv - Biophysics 2019Quote: ... An ÄKTA-Pure liquid chromatography system (GE Healthcare Life Sciences) with a 5mL HisTrap column was used for IMAC purification of the protein ...
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bioRxiv - Microbiology 2021Quote: ... Using an Akta Pure FPLC (GE Healthcare BioSciences, Marlborough, MA) housed at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... using an Äkta Pure chromatography system (GE Healthcare, Pittsburgh, USA). LC-MS and SDS-PAGE was performed to assess purity and integrity of all purified or modified proteins ...
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bioRxiv - Molecular Biology 2023Quote: ... using the AKTA pure 25 system (GE Healthcare, Life sciences). Purified rAAVs were subsequently concentrated using centrifugal concentrators (Amicon Ultra-15 and Ultra-0.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... for SEC on a HPLC ÄKTA Pure system (GE Healthcare) in lysis buffer containing 1 mM DTT.
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bioRxiv - Biochemistry 2023Quote: ... 50 mM NaCl using an ÄKTA pure system (GE Healthcare).
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bioRxiv - Microbiology 2024Quote: ... connected to an ÄKTA Pure system (GE Healthcare, Chicago, USA) using buffer C ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were run on an ÄKTA Pure 25 (GE Healthcare) fast protein liquid chromatography machine at 1 mL/min in PBS pH 7.4 at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Glutathione Sepharose™ 4B (GE healthcare; catalog no. 17-0756), equilibrated to the GST lysis buffer (50 mM Tris-HCl ...
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bioRxiv - Bioengineering 2019Quote: ... 6% Ficoll PM-400 (17-0300-10, GE Healthcare, Sweden), 0.2% sarcosyl (L7414 ...
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bioRxiv - Immunology 2020Quote: ... Elution from HisTrap columns (GE Life Sciences # 17-3712-05) was accomplished using 0.5 M imidazole and fractionated into 1 ml aliquots using the Frac30 fraction collector (GE Life Sciences # 29023051) ...
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bioRxiv - Systems Biology 2020Quote: ... and purified using NAP5 columns (GE Healthcare 17-0853-01). Then maleimide activated antibodies were mixed with 6-15 equivalent of the reduced form of the thiol-modified DNA oligonucleotides in 1× PBS at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... 40 μL Glutathione-Sepharose 4B (GE Healthcare, 17-0756-01) was added to the solution ...
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bioRxiv - Cancer Biology 2022Quote: ... and resuspended in 30% Percoll (GE Healthcare, 17-0891-01) solution containing 10% FBS (Hyclone SH30396.03) ...
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bioRxiv - Microbiology 2022Quote: ... The Percoll (Catalog No. 17-0891-09, GE Healthcare, Sweden) gradient centrifugation method was used for separation and purification ...
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bioRxiv - Immunology 2021Quote: ... Ficoll-Paque Plus (GE-17-1440-03, GE Healthcare, Sweden) purified PBMC were cultured in 96-well plates for 12 hours in the presence of SARS-CoV-2 Spike peptide pools (PepMix™ SARS-CoV-2 JPT or Biosynthesis in 2 pools) ...
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bioRxiv - Neuroscience 2021Quote: ... or Protein G (monoclonal antibodies, GE Healthcare, 17-0618-01) agarose beads were washed with 1 ml of lysis buffer three times and then rotated with the lysates for 1 hour at 4 °C ...
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bioRxiv - Genetics 2021Quote: ... Streptavidin Sepharose High Performance beads (#17-5113-01, GE Healthcare) were used to immobilize 10 μl (ELOVL2 ...
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bioRxiv - Neuroscience 2020Quote: ... Percoll (#17-0891-01) was from GE Healthcare (Marlborough, MA). HBSS (#14185-052 ...
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bioRxiv - Developmental Biology 2021Quote: ... 25μL of Glutathione Sepharose 4B (17-0756-01, GE Healthcare) was blocked with 1% BSA (in Buffer A ...
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bioRxiv - Neuroscience 2022Quote: ... GammaBind Plus Sepharose (17-0886-01) was from GE healthcare. Other chemicals were from Sigma (St ...
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bioRxiv - Neuroscience 2022Quote: ... Protein G Sepharose beads (GE Healthcare CAT#17-0618-01) were washed 3x in RapiGest elution buffer and conjugated to anti-PC antibodies (Novus ...
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bioRxiv - Immunology 2022Quote: ... and resuspended in 30% Percoll (GE Healthcare, 17-0891-01) solution containing 10% FBS (Hyclone SH30396.03) ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 µl Protein G beads (17-0618-02, GE Healthcare) were added to each sample and incubated for an additional hour at 4°C ...
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bioRxiv - Genomics 2023Quote: ... or protein-G-sepharose beads (GE Healthcare, 17-0618-01) for 1h at 4˚C ...
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bioRxiv - Cell Biology 2023Quote: ... Protein-A conjugated sepharose beads (GE Healthcare, 17-0780-01) were added and incubated at 4°C for 2 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 µL streptavidin bead slurry (GE Healthcare, 17-5113-01) per pulldown were either mock treated or blocked with 1 mM biotin by incubation in Buffer C (420 mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... 300 μM 5’ thiol-modified 18-nt DNA oligonucleotides—comprised of an AAA base sequence and a 15-base secondary probe binding sequence (Eurofins)—were reduced with 50 mM dithiothreitol in PBS at room temperature for 2 hours and purified using NAP5 columns (GE Healthcare 17-0853-01). Maleimide-activated antibodies were then combined with 11 equivalents of the reduced form of the thiol-modified DNA oligonucleotides in PBS and incubated at 4℃ overnight ...
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bioRxiv - Molecular Biology 2019Quote: ... concentrated to a volume of 2 ml and applied onto a S75 16/600 pg size exclusion column (GE Healthcare), equilibrated with 10 mM HEPES pH 7.2 ...
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bioRxiv - Immunology 2020Quote: ... Eluted protein was concentrated down to 2 mL for size exclusion chromatography with a Superose6 16/600 column (GE Healthcare) in 10 mM Tris pH8 ...
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bioRxiv - Biochemistry 2022Quote: ... 16/600 (GE Healthcare, Rosenberg, Sweden). The size exclusion chromatography as last step of the purification was performed directly in NMR buffer.
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bioRxiv - Biophysics 2022Quote: ... and analysed by electrophoresis in a blue native gel containing 4 - 16% gradient of acrylamide (29:1 Acrylamide:Bis) using the SE 600 Vertical electrophoresis system (GE Healthcare Life Sciences). The gels were run at 4 °C at constant 200 V for 2 h followed by 6 h at 600 V with max ...
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bioRxiv - Cell Biology 2020Quote: ... Size-exclusion chromatography was performed at 4 °C using a HiLoad™ Superdex 200pg 16/600 GE preparative column (GE Healthcare, USA). The column was equilibrated with 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... All proteins were further purified using Superdex 75 16/60 or Superdex 200 16/60 (GE Healthcare) gel filtration chromatography and stored in their respective running buffers ...