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Citations for GE Life Sciences :
201 - 250 of 3998 citations for Enterovirus CoxB3 VP1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Equal volumes of cell lysates containing ∼1000 μg protein were precleared with 10 μl Protein A Sepharose (GE Healthcare, Freiburg, Germany) for 1 h at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... lysates were incubated with the primary antibody at 4°C for 2 h or overnight and then incubated with protein G- or protein A-Sepharose (GE Healthcare) for 2 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein quantification was performed using BCA and proteins was separated on SDS-PAGE followed by transfer to PVDF membrane (GE Healthcare). Next ...
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bioRxiv - Neuroscience 2023Quote: Proteins from the samples were purified by a modified trichloroacetic acid protein precipitation procedure (Clean-Up Kit; GE Healthcare, Munich, Germany), and gel-assisted proteolysis was carried out ...
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bioRxiv - Immunology 2024Quote: ... The antibody was purified from culture supernatant by protein G affinity chromatography using the ÄKTA Pure system with a HiTrap Protein G column (GE Healthcare).
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bioRxiv - Physiology 2019Quote: ... Equal loading of protein content was verified using alpha-tubulin as a housekeeping protein and bands visualised using ECL detection system (GE Healthcare, UK). Bands were measured using Image J densitometry ...
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bioRxiv - Cell Biology 2021Quote: ... Postnuclear supernatants were incubated with 3 μg of antibodies coupled to protein A– or protein G–Sepharose beads (GE Healthcare, Solingen, Germany) for 4 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were eluted with an imidazole gradient and fractions containing the target protein collected using an AKTA Prime system (GE Life Sciences). Protein quality was analysed by Coomassie gel analysis ...
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bioRxiv - Microbiology 2019Quote: ... Protein was then purified using the AKTA protein purification system with a Ni-NTA HisTrap affinity column (GE Healthcare GE17-5248-02), followed by passage through a Superdex 75 column for size exclusion chromatography ...
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bioRxiv - Immunology 2020Quote: ... Expression of the recombinant proteins was performed using ExpiCHOTM Expression system (Thermoscientific, USA) following purification using HiTrap Protein-A column (GE healthcare, UK). The purified protein was sterile-filtered and stored in PBS.
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bioRxiv - Biophysics 2022Quote: ... The fusion protein was purified by fast protein liquid chromatography (FPLC) using a 5ml HisTrap HP nickel column (GE Healthcare, Uppsala, Sweden) with 0.5M imidazole gradient (SKU I5513-100G ...
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bioRxiv - Cancer Biology 2023Quote: ... Escherichia coli cells by induction with 0.5 mmol/L isopropyl-β-D-thiogalactopyranoside overnight at 16°C and purified recombinant proteins using an ÄKTA fast protein liquid chromatography system (GE Healthcare). This was followed by affinity and size exclusion chromatography.
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: Non-specifically binding proteins were cleared from each sample by incubation with 60 µL of 20% Protein A-Sepharose™ CL-4B (GE Healthcare) in IP Buffer (150 mM NaCl / 1% Triton X-100 / 15 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2021Quote: ... protein was transferred to nitrocellulose membrane (GE healthcare, RPN203D). Membranes were blocked for 1 hour at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were visualized using ECL detection reagents (GE Healthcare). Uncropped gel images for western blots are available in Supplementary information 3.
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bioRxiv - Cell Biology 2019Quote: ... proteins were transferred to nitrocellulose blotting membrane (GE Healthcare). The membrane was blocked with 3% w/v dried skimmed milk (Marvel ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred on 0.45μm nitrocellulose membrane (GE Healthcare), blocked with 5% milk with PBS-Igepal (PBS + 0,3% Igepal ...
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bioRxiv - Plant Biology 2020Quote: ... Tagged proteins were bound on HisTrap (HP)(GE Healthcare) and eluted with increasing imidazole concentration ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Protein A-Sepharose 4 Fast Flow suspension (GE Healthcare) was washed with Tris-Buffered Saline (TBS ...
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bioRxiv - Immunology 2020Quote: ... using protein A affinity chromatography (GE Healthcare, Marlborough, MA). IgG from pooled samples that were collected from the macaques before immunization was purified as the control for passive antibody transfer ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein G beads from GE Healthcare (GE Healthcare, 10280243) were used to purify the target protein and then the protein sample was analyzed using western blot ...
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bioRxiv - Developmental Biology 2021Quote: ... Resolved proteins were transferred into nitrocellulose membranes (GE Healthcare). The following primary antibodies were incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were then transferred on PVDF membranes (GE Healthcare) and detected after incubation with specific antibodies using ECL Clarity Western blotting Substrate (Bio-Rad) ...
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bioRxiv - Genomics 2020Quote: ... Protein G Sepharose beads (GE Healthcare, 4 Fast Flow) were washed with WB140 ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein was bound to glutathione affinity resin (GE Healthcare), washed with 50 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were transferred to hybond ECL membrane (GE Healthcare) and blotted with IL10Rα (AF-474-SP ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 μL of Protein G-sepharose beads (GE Healthcare) coated with 12 µL rabbit anti-Bicc1 ...
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bioRxiv - Cancer Biology 2019Quote: ... using a ÄKTA Purifier protein purification system (GE Healthcare). His-tagged proteins were eluted with a buffer containing 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Bioengineering 2020Quote: ... on an ÄKTA FPLC protein purification system (GE Healthcare) was used to further purify the Nbs and ensure their monomeric states ...
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bioRxiv - Biochemistry 2020Quote: ... Protein samples were incubated with dithiothreitol (DTT -GE Healthcare) at 10 mM and 30°C for one hour ...
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bioRxiv - Biophysics 2021Quote: ... in an AKTA go protein purification system (GE Healthcare). The purified FRET constructs were divided into 200 μL aliquots ...
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bioRxiv - Cell Biology 2021Quote: ... and proteins transferred onto nitrocellulose (GE Healthcare, Chicago, IL) or polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2021Quote: ... 5 µl samples of Protein A-Sepharose (GE Healthcare) were preincubated with 10 µl each of purified α-MyoE or α-Uso1 antibodies for 3 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Protein was transferred to a PVDF membrane (GE Healthcare) and blocked with 5% (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... and Protein A SepharoseTM 4 Fast flow (GE Healthcare) beads by dimethyl pimelimidate cross-linking as described in (69).
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bioRxiv - Plant Biology 2021Quote: ... Separated proteins were transferred to nitrocellulose membrane (GE Healthcare). For BN-PAGE ...
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bioRxiv - Plant Biology 2021Quote: ... dilution) together with protein A-Sepharose beads (GE Healthcare) were used ...
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bioRxiv - Biophysics 2020Quote: ... and purified by protein A affinity chromatography (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... proteins were transferred onto nitrocellulose membranes 0.2μm (GE Healthcare) using a wet electrophoretic transfer ...
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bioRxiv - Biochemistry 2022Quote: ... the protein was purified with Source-Q (GE Healthcare) and Sephacryl S100 columns (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... and protein concentration was measured spectrophotometrically (NanoVue, GE Healthcare). The panning process was described in detail in our previous protocol [64].
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bioRxiv - Cell Biology 2022Quote: ... the proteins were transferred onto PVDF membranes (GE Healthcare) using 25 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... bound proteins were detected by ECL reagent (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... and by HRP-conjugated protein A (GE Healthcare, NA9120V). Colorimetric reaction was started by adding BM Blue POD substrate (Roche ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were incubated with Protein A Sepharose (GE Healthcare) for 1-2 hr at 4°C and bound proteins were washed extensively with lysis buffer and eluted with SDS sample buffer with boiling.
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were transferred to Nitrocellulose blotting membranes (GE Healthcare) at 400 mA for 70 min on ice in cold transfer buffer (25 mM Tris base ...
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bioRxiv - Genomics 2022Quote: ... were bound to protein A magnetic beads (GE Healthcare) and then incubated with MNase extracts over night at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein was bound to glutathione affinity resin (GE Healthcare), eluted by cleavage of the GST-tag with TEV ...
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bioRxiv - Biochemistry 2019Quote: ... PreScission proteinase (8 units/mg of protein, GE Healthcare) was added to remove the His6 tag from the Asf1 ...
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bioRxiv - Cell Biology 2020Quote: ... the protein was purified on Heparin column (GE Healthcare), eluted by NaCl gradient elution and dialyzed for 2 days (20 mM phosphate buffer ...