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Citations for GE Life Sciences :
501 - 550 of 1203 citations for En 1948 4 Who Dioxin Like Pcb "Starter Kit" since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... injection of 185 MBq [123I]-2ß-carbometoxy-3ß-[4-iodophenyl]-N-[3-fluoropropyl]nortropane ([123I]FP-CIT, GE Healthcare, Amersham, UK). The SPECT data acquisition was begun after exactly 3 hours ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were cleared for 30 min at 12,500 × g at 4°C and the supernatant was collected and fractionated on a 1 ml Q-HiTrap column (GE Healthcare) equilibrated in 20 mM Tris ...
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bioRxiv - Neuroscience 2022Quote: ... Aβ42 peptides in the filtrate were further concentrated to ∼50 μM at 4°C and 4000×g with a 5 000 Da Vivaspin concentrator (GE Healthcare). The Aβ42 peptide concentration was calculated using an extinction coefficient of 1400 M-1cm-1 ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was centrifuged at 4 °C and 20000 g for 30 min and the supernatant applied to a gravity flow column with 4 mL pre-equilibrated Ni-NTA Sepharose resin (GE Healthcare). The column was washed with 50 mL of high-salt Tris-HCL buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Neuroscience 2022Quote: ... 1 mg of total protein was pre-cleared for 3h at 4°C by incubation with glutathione-coupled sepharose beads (GE Healthcare) previously equilibrated in Wash buffer 1 (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... Aggregates were removed by centrifugation (20 min, 48000xg, 4° C) and the protein solution loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated in 20 mM MOPS-NaOH pH 7.0 ...
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bioRxiv - Biophysics 2022Quote: ... proteins were concentrated by ultrafiltration at 4 °C (MWCO: 10 kDa) and passed over a Superdex 75 26/60 gel filtration column (GE Healthcare) equilibrated in 20 mM sodium phosphate pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... Radioactive signal was visualized by exposing 4 h a Storage Phosphor Screen BAS-IP MS 2040 (Fujifilm) then imaged with a Typhoon FLA 9500 (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... were acquired every 1–3 min over a total time period of 4–6 h and deconvolved using the softWoRx software (Applied Precision). In addition ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 4 °C) and the supernatant was incubated with 75 μl of IgG Sepharose 6 Fastbeads (GE Healthcare, 52-2083-00 AH) by rotating at 4 °C for 2 to 3 hours to enable binding of the tagged protein ...
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bioRxiv - Synthetic Biology 2022Quote: ... The lysates were clarified by centrifugation at 20 000 × g for 1 hour at 4 °C before loading on a 5 mL HiTrap FF Ni-NTA column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Plant Biology 2022Quote: ... The lysate was centrifuged at 15557 g for 30 min at 4°C and the supernatant was loaded on a HisTrap column (GE Healthcare) equilibrated in 20 mM HEPES 150 mM NaCl 20 mM imidazole 0.02% NaN3 pH = 7.4) ...
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bioRxiv - Plant Biology 2022Quote: ... Purification tags were then removed by incubating with 3C protease (10 μg/mg fusion protein) overnight at 4°C followed by passing through tandem Ni2+-NTA and MBP Trap HP columns (GE Healthcare). The flow-through was concentrated as appropriate and loaded on a Superdex 75 26/600 gel filtration column for final purification and buffer exchange into 20 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2022Quote: ... to 3 mL for loading onto a 1 × 66 cm gel filtration column comprised of 4 g of degassed Sephadex G-50 beads (GE Healthcare) preequilibrated in buffer A O/N ...
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bioRxiv - Microbiology 2022Quote: ... The sample was incubated at 4°C for 30 minutes and further purified using a HiLoad 26/600 Superdex 200 (GE healthcare).
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bioRxiv - Plant Biology 2022Quote: ... coli strain BL21 with protein induction when OD600 reached 0.5 with 1 mM IPTG (isopropyl b-D-1-Thiogalactopyranoside) for 4 h at 37°C and then purified over a glutathione Sepharose 4B column (GE Healthcare). The eluted samples were then resolved by SDS-PAGE and stained by Coomassie brilliant blue R-250 to evaluate purity ...
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bioRxiv - Molecular Biology 2022Quote: ... the pre-cleaning step was omitted and the ChIP extract directly incubated o/n on a rotor at 4°C with 50 μl IgG Sepharose 6 Fast Flow beads (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... After sonication the lysate was clarified by centrifugation (22 500 rpm/61 236 x g, 20 min, 4 °C) and applied to a HisTrap FF column (GE Healthcare) equilibrated with 10 column volumes (CV ...
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bioRxiv - Immunology 2023Quote: ... as binding of DH270 UCA chimeric antibodies containing CDRH3 loops identified from the natural BCR database (Figure 4) was analyzed by Surface Plasmon Resonance on a BIAcore 3000 instrument (GE Healthcare). Biotinylated SOSIP Env 10.17DT was immobilized on a CM5 chip coated with streptavidin ...
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bioRxiv - Neuroscience 2023Quote: ... lysates were incubated with the primary antibody at 4°C for 2 h or overnight and then incubated with protein G- or protein A-Sepharose (GE Healthcare) for 2 h ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions that contained BED were concentrated and further purified at 4 °C on a preparative Superdex 75 16/600 size exclusion column (GE Healthcare) equilibrated with 25 mM HEPES.Na pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions that contained the protein were concentrated and further purified at 4 °C on a preparative Superose 6 XK 16/70 size exclusion column (GE Healthcare) equilibrated with 25 mM HEPES.Na pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The suspension was clarified by centrifugation at 30,000 x g (Sorval SLA 1500) at 4 °C for 30 min and loaded onto a 1 ml HisTrap HP column (GE Healthcare) on an ÄKTA purifier 10 system (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... All chromatography procedures were carried out at 4°C using either an ÄKTA express or ÄKTA pure chromatography system (GE Healthcare). 1 mL StrepTrapTM HP (cat# 28907546 ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was centrifuged for 30 min at 30000 x g at 4°C and loaded onto a Ni-NTA affinity chromatography column (HisTrap FF crude, GE Healthcare). His-tagged proteins were eluted with a linear gradient of buffer B (50 mM Hepes pH 8 ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was centrifuged for 30min at 30000 x g at 4°C and loaded onto a Ni-NTA affinity chromatography column (HisTrap FF crude, GE Healthcare). His-tagged proteins were eluted with a linear gradient of buffer B (PBS 1X ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was centrifuged for 1h at 20000 x g at 4°C and loaded onto a Ni-NTA affinity chromatography column (HisTrap FF crude, GE Healthcare). His-tagged protein was eluted with a linear gradient of buffer B (50 mM Hepes pH 7 ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was centrifuged for 30min at 30000 x g at 4°C and loaded onto a Ni-NTA affinity chromatography column (HisTrap FF crude, GE Healthcare). The His-tagged protein was eluted with a linear gradient of buffer B (Hepes 50 mM pH 8 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were filtered and then pelleted at 300 g for 10 minutes at 4°C before being resuspended in 22% Percoll (GE Healthcare) and centrifuged at 900 g for 20 minutes with acceleration set to 4 and deceleration set to 1 in order to remove cellular debris ...
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bioRxiv - Molecular Biology 2023Quote: ... and 20-30 μg of protein was electrophoresed on 4-12% (or 12% for OGG1 blot) Bis-Tris gels (Thermo) before transferring to PDVF or nitrocellulose membranes (GE Healthcare). For PAR 10H western blots ...
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bioRxiv - Biochemistry 2023Quote: ... Gels were run at 4 °C overnight as described above and scanned with GE Amersham Typhoon-5 fluorescence gel and blot scanner (GE Healthcare) with red laser.
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bioRxiv - Cell Biology 2023Quote: ... and indicated prey proteins at a 5X molar excess to tepsin (mATG8 and/or AP-4 β4 appendage) were incubated with glutathione sepharose resin (GE Healthcare) for 2 hours at 4°C in 20 mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2023Quote: ... the N-terminal His tag was cleaved by overnight dialysis with TEV protease at 4 °C and purified by size exclusion chromatography (SEC) (HiLoad Superdex-200 16/600 column, GE Healthcare) in CHIP storage buffer (50 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... The mixture was incubated at room temperature for 4 hours and was purified using gel filtration chromatography (ÄKTA with Superdex-200 increase column; GE Healthcare) equilibrated with storage buffer ...
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bioRxiv - Biophysics 2023Quote: ... 3,000 g × 15 min at 4 °C) and further purified by size-exclusion chromatography (ÄKTA pure system, Superdex-75 Increase 10/300 GL, GE Healthcare). The unfolding and refolding process for GlnBP WT was conducted under the same conditions as described for the GlnBP variants.
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 50,000 × g for 1 h at 4 °C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... 4°C) and the soluble proteins with His6 tag were purified on 1ml of nickel phosphate loaded sepharose beads (GE Healthcare). Bound proteins were washed in lysis buffer with 25mM imidazole then with 400mM Imidazole ...
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bioRxiv - Biophysics 2023Quote: ... Q7 or Q20 at a concentration of ~4-7mg/ml were loaded onto an S200 Increase size-exclusion column (Superdex 200 Increase10/300 GL, GE Healthcare) at a flow rate of 0.5 ml min-1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell extracts were then incubated over night at 4 °C with appropriate antibodies (1ug/ml) and protein A Sepharose beads (GE Healthcare). After incubation samples were washed five times with lysis buffer and resuspended in 1X Laemmli sample buffer (Bio-Rad cat ...
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bioRxiv - Synthetic Biology 2022Quote: ... The gels were dried (Hoefer GD2000) and exposed for at least 4 days before imaging on a Typhoon FLA 700 (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... The supernatant of the cell lysate was incubated overnight at 4°C on a wheel with Glutathione Sepharose 4B resin (GSTrap 4B, GE Healthcare). After several washes ...
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bioRxiv - Microbiology 2023Quote: ... The clarified lysate was incubated (overnight, at 4 °C, with gentle agitation) with 1 ml bed volume of GSH-Sepharose (GE Healthcare). Next ...
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bioRxiv - Biochemistry 2023Quote: ... Insoluble fractions were removed by centrifugation at 20000 g during 30 min at 4°C and the supernatant was loaded on a 1 mL HisTrapTM HP column (GE Healthcare) connected to a NGC chromatography system (BioRad) ...
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bioRxiv - Cell Biology 2023Quote: ... actin Ac-74 or Pol II S5P) overnight at +4°C and immune-complexes were collected with 50 μl of protein A sepharose (GE Healthcare) at +4°C for two hours on rotation ...
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bioRxiv - Biophysics 2024Quote: ... The sample was centrifuged (10 min, 20000g, 4 °C) and applied to Superdex 200 10/300 gel-filtration column (GE Healthcare) equilibrated with 20 mM Tris-HCl ...
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bioRxiv - Physiology 2024Quote: ... Two-dimensional speckle-tracking echocardiography longitudinal strain curves were derived using RV apical 4-chamber views (EchoPAC, GE Healthcare, Wauwatosa, WI). RV endocardial borders were manually traced ...
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bioRxiv - Biophysics 2023Quote: ... protein samples were purified by size-exclusion chromatography at 4°C using a Superose 6 10/300 GL column (GE Healthcare), equilibrated with a buffer containing 0.03% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... Eluted protein was digested with Ulp1 protease at 4°C for 2 h and then further purified on a Heparin HP column (GE Healthcare), eluting with a linear gradient of increasing NaCl concentration from 300 mM to 1 M in 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... Eluted protein was digested with Ulp1 protease at 4°C for 2 h and then further purified on a Heparin HP column (GE Healthcare), eluting with buffer containing 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... hDrp1 was eluted by cleavage from the affinity resin using 30 mM DTT at pH 8 for 48 hours at 4 °C and further purified using anion exchange chromatography (HiTrap Q FF, GE Healthcare) at pH 8 ...