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Citations for GE Life Sciences :
51 - 100 of 5991 citations for Dickkopf related protein 1 DKK 1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... adjusted to 0.15 M NaCl and 1 % (v/v) Triton X-100 and were applied to a 1 mL Hi-Trap heparin column (GE Healthcare Life Sciences) equilibrated with a modified phosphate-buffered saline (PBS) ...
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bioRxiv - Genetics 2019Quote: ... The final antisera were purified separately using His-DBN-1(1-132) bound to NHS-activated Sepharose High Performance (GE Healthcare Life Science). Both purified antibodies were tested in western blot ...
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bioRxiv - Biophysics 2019Quote: ... the protein was further purified using ion-exchange Hi-Trap Q HP-(GE Healthcare). The protein quality was assessed by SDS-PAGE and analytical size-exclusion chromatography using the Superpose 10/300 column (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... The dialyzed protein was then passed through a Hi-Trap Chelating column (GE Healthcare) and the unbound material was collected ...
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bioRxiv - Plant Biology 2020Quote: ... tNPR1-His proteins were further purified by HiLoad 16/600 Superdex 200 (GE Healthcare) with 200 mM NaHCO3 ...
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bioRxiv - Neuroscience 2022Quote: ... together with a 5 ml HisTrap HP His tag protein purification column (GE Healthcare). The supernatant was loaded onto the column initially washed with 25 ml buffer A ...
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bioRxiv - Bioengineering 2022Quote: ... We purified the N-terminal His-tagged proteins on a HisTrap column (GE Healthcare) and then by size exclusion chromatography (SEC ...
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bioRxiv - Cell Biology 2019Quote: ... The supernatant fraction containing proteins were loaded on 5ml His-Trap column (GE Healthcare) and eluted with a linear gradient of elution buffer containing 40mM to 500mM Imidazole ...
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bioRxiv - Cell Biology 2021Quote: ... His-tag fusion proteins were purified from lysates using HisTrap HP column (GE Healthcare). The size and purity of the protein preparations were verified by SDS-PAGE and Coomassie Blue staining ...
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bioRxiv - Biochemistry 2022Quote: ... and His(6)GFP –AnkBD proteins were purified using Glutathione Sepharose 4B (GE Healthcare) or Talon® (Clontech ...
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bioRxiv - Biochemistry 2022Quote: ... the His-tagged DnaK protein was purified using a HisTrap HP column (GE Healthcare) with 2×PBS buffer followed by further purification using a HiTrap Q column (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... The purified protein was desalted using a His Trap Desalting columns from GE Healthcare according to the prescribed protocol and was stored at −80°C with 40 % glycerol.
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bioRxiv - Plant Biology 2022Quote: ... The His-MDIS1CD protein is loaded onto a Ni-NTA agarose column (GE Healthcare) and eluted using elution buffer containing 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... coli as His-tag proteins and were purified using the IMAC principle (GE healthcare). Following purification ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were purified by Ni-affinity chromatography on 5 mL His-Trap columns (GE Healthcare) as follows ...
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bioRxiv - Biochemistry 2019Quote: ... The recombinant protein was purified under denaturing conditions over a His-SpinTrap column (GE Healthcare), desalted using a PD SpinTrap G-25 column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tag was removed upon incubation of the target protein with thrombin (GE Healthcare) at room temperature for 16 h ...
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bioRxiv - Immunology 2021Quote: ... RBD protein was then captured using His-Trap HP column (GE Healthcare, Wauwatosa, WI, USA) or HiTrap TALON crude column (Cytiva ...
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bioRxiv - Immunology 2020Quote: ... the His-tagged proteins were purified using nickel-affinity chromatography (HiTrap chelating, HP; GE Healthcare) with an imidazole gradient (10-250 mM ...
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bioRxiv - Molecular Biology 2020Quote: ... The His-tagged proteins were purified by cobalt affinity chromatography using Chelating Sepharose (GE Healthcare). The proteins were eluted in buffer containing 25 mM Hepes-KOH (pH 7.4) ...
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bioRxiv - Immunology 2022Quote: ... The His-tagged proteins in the supernatants were purified using HIStrap FF columns (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... His-tagged proteins were purified from transient transfection supernatants on HisTrap HP columns (GE Healthcare) using an ÄKTAxpress (GE Healthcare ...
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bioRxiv - Biophysics 2024Quote: ... the protein mixture was loaded into a 5 ml His-trap FF column (GE Healthcare) pre-equilibrated with buffer B ...
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bioRxiv - Immunology 2021Quote: ... Cell lysates were centrifuged at 12,000 x g for 30 min and the supernatant was subsequently used for protein purification through a HisTrap column (His- tagged full-length proteins, GE Healthcare) or Amylose resin (MBP-tagged fragments ...
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bioRxiv - Molecular Biology 2022Quote: His-Prs was purified on His-Trap columns (GE Healthcare) as described above and dialyzed overnight at 20 °C into 1 x acetylation buffer (100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... Affinity chromatography on Hi-Trap His Column (54835, GE Healthcare) was used as a first step as a 6x His-tag was added at the amino terminal domain of IF2 ...
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bioRxiv - Immunology 2021Quote: ... Human antibodies purified by Protein-G (GE Healthcare, USA) affinity chromatography were stored at -80°C until use.
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bioRxiv - Cell Biology 2019Quote: ... His-TWF-1 was further purified with Superdex-75 increase 10/300 GL gel filtration column (GE Healthcare) equilibrated 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant corresponding to His-JMJD6 (1-343) was loaded to 7mL of Ni-NTA resin (GE Healthcare), washed with nickel-binding buffer containing 20mM imidazole ...
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bioRxiv - Biochemistry 2019Quote: ... Enzymes were purified from the cell lysate using a 1 ml His-Trap FF crude column (GE Healthcare) using an elution gradient from 10 to 250 mM imidazole in 25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... The lysate supernatant was than loaded on a Ni-NTA column (GE Healthcare, His Trap FF 1 ml) pre-equilibrated with a binding buffer (8 M Urea ...
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bioRxiv - Microbiology 2020Quote: ... MS3_01370 was purified by IMAC by loading onto a prepacked 1 ml His-Trap HP column (GE Healthcare) equilibrated with lysis buffer at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... Filtered lysate was applied to a 1-ml His-Trap (all FPLC columns were obtained from GE Healthcare) column using an ÄKTA FPLC ...
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bioRxiv - Cell Biology 2021Quote: ... His⨯6-tagged proteins in the supernatant were purified by Ni-NTA agarose beads (GE Healthcare). After extensive wash by a buffer containing 20 mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... His-tagged proteins were purified from supernatants on HisTrap HP columns using an ÄKTApure (GE Healthcare) and resolved by gel filtration on a Superdex 200 Increase 10/300 column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tagged fusion proteins were purified by Ni2+-Sepharose 6 fast flow (GE Healthcare, USA) affinity chromatography followed by size-exclusion chromatography.
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bioRxiv - Biochemistry 2019Quote: ... His-tag removed protein was further purified by size-exclusion chromatography (S200 16/60; GE Healthcare) in buffer containing 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2019Quote: ... His-tagged recombinant protein was purified by TALON Superflow (GE Healthcare Life Sciences, Pittsburgh, PA, USA) according to the manufacturer’s description ...
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bioRxiv - Microbiology 2019Quote: ... The soluble His-tagged protein was purified using a HisTrap column (GE Healthcare Life Sciences, USA). Purity of the eluted protein was checked by SDS-PAGE gel staining with Coomassie Blue ...
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bioRxiv - Cell Biology 2019Quote: Proteins were purified according to their His6-tag using a His Excel column from GE Healthcare attached to a Äkta Start system ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were purified using the His GraviTrap column (GE Healthcare Life Sciences, 11-0033-99) by following the manufacture’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... The protein sample was passed through His Trap™ FF crude column (GE Healthcare, Piscataway, NJ). We expected the undigested protein to remain bound to the column ...
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bioRxiv - Biochemistry 2024Quote: ... The free dye was separated from labelled protein by a Hi-TRAP Desalting column (GE Healthcare). The final degree of labelling of FtsZ and ZapD was calculated from the molar absorption coefficients of the protein and the dye ...
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bioRxiv - Cell Biology 2020Quote: Stathmin was purified using His-affinity (His-Trap HP, GE Healthcare) by first binding in binding buffer (20mM NaPO4 pH 8.0 ...
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bioRxiv - Cell Biology 2019Quote: ... The supernatant was loaded at 1 ml/min on a His-Trap FF-crude 5 mL column (GE Healthcare) equilibrated with PBS buffer ...
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bioRxiv - Microbiology 2021Quote: ... and the resulting soluble fraction was applied on a Ni-NTA column (GE Healthcare, His Trap FF 1 ml) and purified according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The soluble extract was loaded onto the Hi-Trap Chelating HP 1 ml pre-packed column (GE Healthcare/Cytiva) pre-equilibrated with buffer A containing 500 mM NaCl ...
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bioRxiv - Immunology 2022Quote: ... The proteins were harvested from the supernatant of cell cultures and purified by HisTrap Excel (for his-tagged proteins) or Protein G (for antibodies) columns (GE Healthcare Life Sciences). RHMS-1 (trimer) ...
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bioRxiv - Cell Biology 2020Quote: XMAP215-GFP-7xHis was purified using His-affinity (His-Trap, GE Healthcare) by binding in buffer (50mM NaPO4 ...
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bioRxiv - Biochemistry 2020Quote: ... and the His-tag of Rab8a (anti-His-Antibody, GE Healthcare, mouse). Both were diluted (1:1000 ...