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Citations for GE Life Sciences :
601 - 650 of 3068 citations for Dechlorane Plus Syn Bis Cyclopentene 13C10 99% 100 Ug Ml In Toluene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... These cells were isolated from human blood by density-based centrifugation following layering of the blood onto Ficoll-Hypaque plus (GE healthcare). Cells were isolated and washed three times in RPMI (Gibco ...
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bioRxiv - Biophysics 2020Quote: ... Human PBMCs were isolated from leukopheresis packs obtained from the Central Blood Bank (Pittsburgh, PA) via Ficoll-Paque Plus (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Biochemistry 2020Quote: ... The target protein was eluted with loading buffer containing 400 mM imidazole and then purified by gel filtration with a Superdex 200 column on the ÄKTAprime plus system (GE Healthcare) in binding buffer (20 mM Tris-Cl ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated for 1 h with 5 μL of anti-MYC antibody (Gentex) followed by 1 h incubation with an additional 50 μL of GammaBind plus Sepharose beads (GE Healthcare) to capture protein complexes ...
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bioRxiv - Cell Biology 2021Quote: ... and fractionated by linear gradient with end buffer (10 mM Tris-HCl, 1 M NaCl) using an AKTAprime plus (GE Healthcare) at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... were collected by crushing of bones (tibias, femurs, hips, humeri and spine) and subjecting the cells to density gradient centrifugation (Ficoll-Paque Plus, GE Healthcare) at 400g for 25 minutes with brakes turned off ...
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bioRxiv - Cell Biology 2022Quote: ... incubated in HRP conjugated secondary antibodies detailed in Table 4 for 1 h at room temperature and developed using Pierce SuperSignal Pico Plus (Pierce) or ECL (GE Healthcare) reagent and imaged on ImageQuant.
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bioRxiv - Cell Biology 2022Quote: ... Bound proteins were visualized using rat anti-HA antibody and detected using peroxidase-labeled anti-rat IgG antibody and ECL Plus (GE Healthcare).
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bioRxiv - Physiology 2022Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from age- and sex-matched mice by centrifugation over Ficoll-Paque Plus (GE Healthcare). Splenocytes were obtained by crushing the tissue through a 70μm nylon filter (Falcon) ...
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bioRxiv - Microbiology 2022Quote: ... or SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo-Fisher-Scientific)) and chemiluminescent signals were recorded with an Image Quant LAS4000 imaging system (GE Healthcare) or an Amersham Imager 680RGB (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: Blood from patients with melanoma was collected in heparinized or EDTA tubes and was diluted with an equal volume of PBS before being layered over a Ficoll Paque PLUS gradient (GE Healthcare) in 50 mL conical tubes that were centrifuged at room temperature for 15 minutes at 932 g ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were detected using Western Lightning Plus-ECL (Perkin-Elmer, NEL104001EA) and images were acquired on an ImageQuant LAS4000 system (GE Healthcare). Densitometry analysis was carried out using ImageQuant TL software.
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bioRxiv - Immunology 2021Quote: Mononuclear cells were isolated from the human umbilical cord blood of healthy neonates (Turku University Central Hospital, Turku, Finland) by Ficoll-Paque density gradient centrifugation (Ficoll-Paque PLUS; GE Healthcare). Naive CD4+ T cells were further purified using CD4+ Dynal positive selection beads (Dynal CD4 Positive Isolation Kit ...
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bioRxiv - Molecular Biology 2022Quote: ... Mononuclear cells (MNCs) were isolated from cord blood cells by centrifugation using Ficoll-PaqueTM PLUS (GE Healthcare Life Sciences, Buckinghamshire, UK). CD34+ cell enrichment was performed using EasySep™ Human CD34 Positive Selection Kit II (StemCell Technologies ...
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bioRxiv - Immunology 2022Quote: ... mononuclear cells were isolated by density gradient centrifugation of PBS-diluted buffy coat/blood (1:2) over Ficoll-Paque Plus (GE Healthcare). The PBMC layer was carefully removed and washed 3 times with PBS.
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bioRxiv - Immunology 2019Quote: Peripheral blood was collected from all subjects in K2EDTA tubes and PBMCs were isolated by density gradient centrifugation using Ficoll-Paque Plus (GE Healthcare). Cells were resuspended in freezing medium containing 90% FBS and 10% DMSO ...
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bioRxiv - Immunology 2020Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation using Ficoll-Paque™ Plus (GE Healthcare Europe, Brøndby, Denmark). They were either examined directly ex vivo or cryopreserved in 10% DMSO and 90% FCS at -150°C for later in vitro analysis.
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bioRxiv - Cancer Biology 2020Quote: Custom siRNAs with and without the ON-TARGET-Plus modification to block seed mediated off-target effects were obtained through Dharmacon (GE Healthcare). When indicated custom siRNAs were also ordered with a Cy5 label on the 3’ end of the passenger strand ...
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bioRxiv - Immunology 2019Quote: Blood was collected at the Northwest Assistance Ministries Clinic (Houston, TX) and PBMCs were isolated from whole blood within 4 h of collection using Ficoll Paque plus (GE Healthcare) and frozen down in 7% DMSO in fetal bovine serum and cooled at −1°C/min in a freezing container (Nalgene ...
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bioRxiv - Cell Biology 2021Quote: ... lymphocytes and monocytes were isolated from heparinized human peripheral blood by Ficoll-PaqueTM PLUS (GE Healthcare Bio-Sciences AB, Uppsala, Sweden) gradient centrifugation ...
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bioRxiv - Molecular Biology 2020Quote: ... and RNA primer 5’-rCrArGrUrGrCrUrArUrGrUr GrArGrArUrUrArArGrUrUrArU-3’ were prepared by solid phase synthesis on an ÄKTA oligopilot plus 10 (GE Healthcare). RNAs were cleaved from the solid support by treating with 4 mL of a 1:1 mixture of 28% wt NH3/H2O solution and 33% wt CH3NH2/EtOH solution at 55°C for 30 minutes ...
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bioRxiv - Immunology 2020Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats by density gradient centrifugation on Ficoll-Paque™ PLUS (1.077 g/L; GE healthcare). Then ...
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bioRxiv - Cell Biology 2020Quote: ... The protein bands were visualised using ECL™ or ECL plus™ reagents and Hyperfilm® ECL (all from GE Healthcare) and detected with a CCD camera (Biorad) ...
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bioRxiv - Cell Biology 2020Quote: ... the corresponding cDNAs of mouse angulin-1 were amplified by PCR using KOD-Plus-Ver.2 DNA polymerase and subcloned into pGEX-6P-1 (GE Healthcare). To construct an expression vector for MBP-tagged N-ZO-1 (aa 1-862) ...
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bioRxiv - Immunology 2020Quote: ... at room temperature and immunoprecipitated protein was detected using Amersham® ECL Plus Western Blot Detection System (GE Healthcare, Buckinghamshire, UK). Radioactivity ...
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bioRxiv - Immunology 2021Quote: ... were isolated from the umbilical cord blood of healthy neonates (Turku University Central Hospital, Turku, Finland) using the Ficoll-Paque density gradient centrifugation (Ficoll-Paque PLUS; GE Healthcare). Naive CD4+ T cells were further purified using CD4+ Dynal positive selection beads (Dynal CD4 Positive Isolation Kit ...
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bioRxiv - Immunology 2020Quote: ... isolated from the buffy coats of healthy blood donors (Stanford Blood Center) were separated by density gradient centrifugation using Ficoll-Paque PLUS (GE Healthcare) followed by 3X washes in HBSS (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were scanned using an infrared imaging system (Odyssey; LI- COR Biosciences) or using ECL Plus Western blotting reagent (Cat. no. RPN2232, GE Healthcare) and film ...
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bioRxiv - Immunology 2022Quote: For in vitro assays PBMCs were isolated using density gradient centrifugation with Ficoll-Paque PLUS (GE Healthcare Bio-Sciences AB, Sweden). Heparinized blood was diluted with phosphate-buffered saline (PBS ...
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bioRxiv - Biophysics 2023Quote: ... Samples were injected on the Äkta prime plus chromatography system (Cytivia) and separated on a Superose 6 10/300 column (GE Healthcare) in SEC buffer (10 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... the membrane is developed with a chemiluminescent substrate (Super SignalTM West Pico PLUS) and visualized in ImageQuant LAS 4000 mini Biomolecular Imager (GE Healthcare).
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bioRxiv - Immunology 2023Quote: ... 6 and 14 we euthanized 5 individuals per group and performed an intra-cardiac terminal blood collection for PBMCs isolation with Ficoll-Paque Plus (GE healthcare). We performed a complete necropsy of all animals and collected samples of the lungs ...
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bioRxiv - Systems Biology 2023Quote: ... were isolated from healthy donor blood (courtesy of the UCLA Blood and Platelet Center) by density gradient centrifugation using Ficoll-Paque PLUS (GE Healthcare). On the day of isolation ...
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bioRxiv - Molecular Biology 2023Quote: ... Detection of immunoreactive bands was performed using the Western Lightning Chemiluminescence Reagent Plus (Perkin-Elmer Life Sciences) and a LAS4000mini CCD camera apparatus (GE healthcare).
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bioRxiv - Bioengineering 2023Quote: ... where 18 mL of blood was mixed with Hanks’ Balanced Salt Solution (HBSS, 14175095, GibcoTM) before being poured onto Ficoll-Paque Plus (GE17-1440-03, GE Healthcare). The layers were separated through centrifugation ...
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bioRxiv - Microbiology 2023Quote: ... from 3 different healthy donors to avoid unique donor bias on type cells effects and extracted using Ficoll-Plaque Plus solution (GE Healthcare). PBMCs were re-suspended in RPMI-1640 medium (Lonza Bioscience ...
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bioRxiv - Immunology 2023Quote: Peripheral blood mononuclear cells (PBMCs) were isolated by centrifugation using a Ficoll-Paque Plus gradient (GE Healthcare, Cat #17-1440-02), then washed by centrifugation ...
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bioRxiv - Immunology 2023Quote: Peripheral blood mononuclear cells (PBMCs) from fresh peripheral blood of healthy donors were isolated by density gradient centrifugation using Ficoll-Paque Plus (GE Healthcare). Cytotoxic T cells and T helper cells were isolated from the PBMCs by positive selection with CD8- and CD4-specific MicroBeads (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2023Quote: ... MNCs were isolated from each sample using the standard gradient separation approach with Ficoll-Paque PLUS (GE Healthcare Lifesciences, Pittsburgh, PA).
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bioRxiv - Immunology 2024Quote: ... Peripheral blood mononuclear cells (PBMCs) or polymorphonuclear cells (PMNs) were isolated from whole blood by density gradient centrifugation using Ficoll-Paque Plus (GE Healthcare) or Polymorphprep (Axis-Shield) ...
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bioRxiv - Cell Biology 2024Quote: ... EGFP-Kif9 motility was observed in TIRF on the growing red plus-end extensions of MTs using a Personal Deltavision microscope (Applied Precision) with 4-laser TIRF capabilities and an Olympus 60X 1.49 NA TIRF objective at 37 °C.
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bioRxiv - Plant Biology 2024Quote: ... Immunodetection was performed using an ECL Plus Western Detection Kit and revealed with an ImageQuant LAS 4000 mini CCD camera system (GE Healthcare). ImageJ was used for quantifications of the bands.
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bioRxiv - Cancer Biology 2024Quote: ... were isolated from human buffy coats from the New York Blood Center by density gradient centrifugation using Ficoll-Paque PLUS (GE Healthcare). Primary NK cells were extracted from PBMCs by negative immunomagnetic isolation using a NK cell isolation kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: PBMCs or PMNs were separated from EDTA-anticoagulated whole blood by density gradient centrifugation either with Ficoll-Paque Plus (GE Healthcare) or Polymorphprep (Axis-Shield) ...
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bioRxiv - Immunology 2024Quote: ... were sourced from buffy coats of deidentified donors (Stanford Blood Center, Palo Alto, CA, USA) and isolated using Ficoll-Paque Plus (GE Healthcare) density gradient ...
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bioRxiv - Neuroscience 2020Quote: ... 1 x 20 mL (GE Healthcare) on an ÄKTA pure protein purification system (Cytiva ...
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bioRxiv - Cancer Biology 2020Quote: ... 5.5 mL Percoll (GE Healthcare, Germany); 0.6 mL 1.5 M NaCl) ...
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bioRxiv - Microbiology 2020Quote: ... GSTrap columns (5 mL; GE Healthcare) were used with an Akta Prime FPLC system (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... 5 ml of HisTrap (GE Healthcare) was used for the affinity purification and Gel filtration was performed on HiLoad 16/60 Superdex 200 (GE Healthcare) ...
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bioRxiv - Neuroscience 2023Quote: ... 1 x 20 mL (GE Healthcare) on an ÄKTA pure protein purification system (Cytiva ...