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Citations for GE Life Sciences :
301 - 347 of 347 citations for DNA Polymerase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... the PCR amplified eleven positive virus genomes were amplified by RCA (rolling circle amplification) using the RCA-based TempliPhi DNA amplification kit (GE Healthcare) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... The digested DNA fragments were separated by electrophoresis using 1% agarose gels and transferred overnight to Hybond-N+ membranes (GE Healthcare) in 20 × SSC ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR products amplified with primers Aeatro-Nix-F1/R1 were purified with GFX PCR DNA and Gel Band Purification Kit (GE Healthcare) and cloned into pJET1.2/blunt cloning vector ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR products amplified with primers Aeatro-Nix-F2/R2 were purified with GFX PCR DNA and Gel Band Purification Kit (GE Healthcare) and directly sequenced.
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bioRxiv - Plant Biology 2023Quote: ... DNA was isolated from leaf tissue of 4-wk-old plants by using Illustra Nucleon Phytopure extraction kit reagents (GE Healthcare). DNA was isolated from agarose gel pieces using QIAquick gel extraction kit (Qiagen).
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bioRxiv - Cell Biology 2023Quote: To create BAC transgenic mice 10 μg maxi-prepped BAC DNA (Nucleobond 100) was linearised by restriction digest (NotI) and purified by sepharose column purification (GE Healthcare). Briefly ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we introduced random mutations with Multi Primed Rolling Circle Amplification using the Illustra™ TempliPhi™ DNA Amplification Kit (GE Healthcare) with 2 mM of MnCl2 added to a final reaction volume of 10.8 µl ...
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bioRxiv - Microbiology 2024Quote: ... to reach 120 mM of NaCl and were further passed through a Hi-Trap Q HP 1 mL column to remove residual DNA before binding on a Hi-Trap heparin HP 1 mL column (GE Healthcare) and elution with a NaCl gradient ...
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bioRxiv - Neuroscience 2024Quote: ... RNA (5 μg) was used to synthesize the first-strand complementary DNA (cDNA) using the First-Strand cDNA synthesis kit (GE Healthcare) with a pd(N)6 primer following the manufacturer’s indications ...
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bioRxiv - Biochemistry 2023Quote: ... After staining with Ethidium bromide to visualize total DNA and gel transfer on a Nylon N+ membrane (GE Healthcare Ref RPN203B) (Qbiogen ...
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bioRxiv - Cell Biology 2024Quote: ... Pek1 and Atf1 were generated by PCR of the corresponding gene fragments from yeast genomic DNA or cDNA and cloned in frame into expression vectors pGEX-4T-1 (GE Healthcare) or pMAL-c2x (New England Biolabs) ...
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bioRxiv - Genetics 2020Quote: ... High molecular weight DNA from concentrated coral sperm was extracted using the illustra Nucleon Phytopure kit (GE Healthcare Life Science, Pittsburgh, PA) following the manufacturer’s instructions and eluted in nuclease-free water ...
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bioRxiv - Cancer Biology 2019Quote: ... Equal amounts of DNA from each specimen were spotted on a piece of Nytran N nylon blotting membrane (GE Healthcare Life Sciences). The membrane was incubated with a primary antibody that recognized Pt-induced DNA adducts (ICR4 ...
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bioRxiv - Plant Biology 2020Quote: ... MKK7 ORF was PCR amplified from Arabidopsis genomic DNA using primers MKK7-F and MKK7-R1 (Table 2) and cloned into pGEX-5X-1 (GE Healthcare, USA) into BamHI-EcoRI restriction sites ...
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bioRxiv - Microbiology 2021Quote: ... according to manufacturer’s instructions and circular DNA was enriched by rolling circle amplification (RCA) with TempliPhi™ amplification kit (GE Healthcare, USA).
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bioRxiv - Cancer Biology 2020Quote: DNA probes complementary to the TP53 coding region or the 3′UTR were labeled with dCTP [α-32P] using the Amersham Megaprime DNA labeling system (GE Healthcare). Primers used for probe synthesis from human cDNA are listed in Supplementary Table 1 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... gene specific fragments that were used for VIGS (primer pairs listed in Table S1) were radiolabeled with [α-32P] dCTP (Perkin-Elmer) using the Rediprime II DNA Labeling System (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... GTP-loaded protein was buffer exchanged into either NMR buffer or the GTPase assay buffer using NAP-5 Sephadex G-25 DNA Grade columns (GE Healthcare # 17085301). We were unable to obtain sufficient material for some mutants (H141E/I ...
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bioRxiv - Molecular Biology 2019Quote: ... random hexamer primers were used following the manufacturer’s instructions for the GenomiPhi V2 DNA Amplification Kit (catalogue # 45-001-221, GE Healthcare Life Sciences).
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bioRxiv - Genetics 2020Quote: We performed WGA on each F2 gDNA cercarial sample using the Illustra GenomiPhi V2 DNA Amplification kit (GE Healthcare Life Sciences, USA) according to Le Clec’h et al ...
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bioRxiv - Genomics 2021Quote: ... G-25 sephadex™ G-50 DNA grade resin columns were used to remove unincorporated γ-32P ATP according to manufacturer’s instructions (illustra™ GE Healthcare). The purified strand was used for subsequent annealing with equal amount of complementary strand in 50 μL total volume ...
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bioRxiv - Molecular Biology 2020Quote: ... and probed with 5’ end-labeled oligonucleotides or PCR fragments labeled by random priming (Megaprime DNA Labeling System, GE Healthcare Life Sciences). Hybridizations were performed in UltraHyb or UltraHyb-Oligo (Ambion ...
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bioRxiv - Genomics 2021Quote: Single-cell WGA was done on 10~17 μm or 17~25 μm C1 DNA-Seq IFC (Fluidigm, 100-5763 or 100-5764) via multiple displacement amplification method using illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare, 25660031). The WGA products from single cells were mixed according to their tissue origin ...
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bioRxiv - Microbiology 2022Quote: ... whole genome amplification was carried out using the multiple displacement amplification technique with the Genomiphi V2 DNA Amplification Kit (GE Healthcare, USA). This approach has been widely used previously in various works [18–20] ...
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bioRxiv - Microbiology 2023Quote: ... All genes used in the curves were purified with the IllustraTM GFXTM PCR DNA and Gel Band Purification Kit (GE Healthcare, UK) for better efficiency of the standard curves ...
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bioRxiv - Genomics 2023Quote: ... 1 μg of fosmid or BAC DNA was labelled by nick-translation with fluorescent dUTPs (Abott Molecular or GE HealthCare Life Science) for 3 h at 15°C as described previously41 ...
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bioRxiv - Biophysics 2019Quote: Synthetic DNA oligonucleotides (Ella Biotech) were internally labelled with a monoreactive NHS-ester form of cyanine dyes as donors (Cy3, GE Healthcare, Cat# PA13101) or acceptors (Cy5 ...
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bioRxiv - Microbiology 2019Quote: ... Samples were incubated at room temperature for 1 h and DNA was extracted with magnetic Sera-Mag Speedbeads (GE Healthcare Life Sciences, UK) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... PCR reactions were analyzed by agarose gel electrophoresis with ethidium bromide staining and purified using the Illustra GFX PCR DNA and Gel Band Purification kit (GE Healthcare, Chicago, IL). Reactions (10 μL ...
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bioRxiv - Molecular Biology 2022Quote: ... Gels were blot-transferred to a nylon membrane (Amersham Hybond-N+ #RPN203B) and probed with pBR322 DNA sequences labelled with AlkPhos Direct (GE Healthcare® #GERPN3680). Chemiluminescent signals of increasing exposition periods were recorded with a cooled CCD camera (KODAK Gel Logic 1500 Imaging System ...
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bioRxiv - Biochemistry 2020Quote: The human Cdc42 1-179aa Q61L (referred to as Cdc42)-encoding DNA was cloned into a modified pGEX-4T-1 vector (GE Healthcare, Chicago, Illinois) as previously described (Barthelmes et al. ...
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bioRxiv - Cell Biology 2020Quote: ... and WAVE2-WCA (419-497) were amplified from plasmid DNA and inserted into BamHI/SalI sites of pGEX-6P-1 (GE Healthcare, Munich, Germany). Plasmids pGEX-6P-2-cortactin (Lai et al. ...
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bioRxiv - Microbiology 2022Quote: ... the DNA fragments were subsequently excised using BamHI and SalI and then inserted into the same sites in pGEX4T2 (GE Healthcare, Piscataway, NJ) (32) ...
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bioRxiv - Immunology 2023Quote: ... The products were filtered by 10 kDa-cutoff Amicon Ultra centrifugal filters to remove DNA and proteins and then analyzed by a Mono Q 5/50 GL column (GE Healthcare Life Sciences).
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bioRxiv - Bioengineering 2024Quote: ... 2 μg DNA loaded per lane was separated by electrophoresis on 1% agarose gels and transferred to Hybond-N membranes (GE Healthcare, Chicago, IL) using capillary flow transfer method ...
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bioRxiv - Genomics 2020Quote: ... 2 μL of each field sample was subject to whole genome amplification using the illustra Genomiphi V2 DNA amplification kit (GE Healthcare Life Sciences, Australia) for 2 hours at 30°C followed by heat inactivation at 65°C for 10 minutes ...
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bioRxiv - Plant Biology 2021Quote: ... DNA fragments were separated on the 0.8% agarose gel and alkali-blotted onto a Hybond XL membrane (GE Healthcare Life Sciences, Waukesha, WI, USA). Telomere fragments were detected by a radioactively labeled telomeric DNA probe ...
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bioRxiv - Molecular Biology 2020Quote: ... 4.5 µL of Cy5 Mono-Reactive NHS Ester Fluorescent Dye (ChIP samples) or Cy3 Mono-Reactive NHS Ester Fluorescent Dye (control samples Input DNA or no-tag ChIP) (GE Healthcare, PA25001 and PA23001) were added ...
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bioRxiv - Microbiology 2022Quote: ... The denatured DNA was then blotted onto a Hybond LFP membrane using a Minifold I dot blot system (both from GE Healthcare, Piscataway, NJ, USA) followed by a wash with 400 µL TE buffer per well ...
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bioRxiv - Cell Biology 2024Quote: ... PCR samples were resolved on agarose gel and amplified fragments extracted from gel using GFX PCR DNA and Gel Band purification kit (GE Healthcare, 28-9034-70). The purified DNA or 1µg of plasmid DNA were digested with XmaI and XbaI for 1 h at 37°C in 1x CutSmart buffer and nuclease-free water ...
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bioRxiv - Microbiology 2021Quote: ... 2.5 μL of the purified DNA was amplified by Multiple Displacement Amplification (MDA) with the Genomephi V3 kit (GE Healthcare Life Science, Marlborough, MA, USA) for 30 minutes ...
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bioRxiv - Immunology 2019Quote: ... and DNA was extracted from peripheral blood mononuclear cells through commercial kits (Qiagen GmbH, Hilden, Germany and GFX™ Genomic Blood DNA Purification Kit, GE Healthcare, São Paulo, Brazil).
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bioRxiv - Evolutionary Biology 2021Quote: ... Probes were labled with 32P-dCTP (Hartmann Analytic, Braunschweig) by the use of the Rediprime II DNA Labeling Kit (GE Healthcare Life Science, Little Chalfont) according to the manufacturer’s manual ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were then extracted and purified using an illustra™ GFX™ PCR DNA and Gel Band Purification kit (GE Healthcare, Buckinghamshire, UK). The samples were sequenced using the general Sanger sequencing technique.
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bioRxiv - Plant Biology 2022Quote: ... chromosomal DNA was purified and amplified to 5.4 - 7.9 μg by multiple displacement amplification (Supplementary Table 3) using an Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare, Chalfont St. Giles, United Kingdom) as described by Šimková et al ...
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bioRxiv - Microbiology 2024Quote: The same mixture of phage DNA as mentioned above was amplified by Multiple Displacement Amplification (MDA) using the Genomephi V3 kit (GE Healthcare Life Science, Marlborough, MA, USA) with 10 ng input and incubated at 30°C for 0.5 h (MDA_0.5h ...
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bioRxiv - Genomics 2019Quote: ... cell lysis and whole-genome amplification steps were carried out in Fluidigm C1 system using illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare Life Sciences cat: 25-6600-30). DNA concentrations of amplified single-cell genomes were determined using either a Qubit dsDNA HS Assay kit (Invitrogen cat:Q32854 ...