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Citations for GE Life Sciences :
1 - 50 of 2633 citations for D Ribitol 5 Phosphate Cytidylyltransferase ISPD CRPPA Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... 250 μM TCEP and 20 mM sodium phosphate at pH 6.5 (ITC buffer) using a 5 mL HiTrap Desalting column (GE Healthcare). Likewise ...
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bioRxiv - Neuroscience 2022Quote: ... and phosphate-buffered saline (PBS, SH302560, HyClone, GE Healthcare).
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bioRxiv - Pathology 2023Quote: ... Phosphate buffered saline (PBS) was purchased from GE Healthcare Life Sciences (Logan ...
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Differentiation Protocol-Dependent Variability in hiPSC-Derived Endothelial Progenitor FunctionalitybioRxiv - Bioengineering 2024Quote: ... in Dulbecco’s Phosphate-Buffered Saline (DPBS, GE Healthcare, SH30028.03), and 2 µL of CD34-PE antibody (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2024Quote: ... rinsed with phosphate-buffered saline (PBS) (GE Life Sciences), permeabilized with 0.25% Triton X-100 (Sigma- Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... After incubation with secondary HRP-conjugated antibody (R&D) blots were developed using ECL reagent (GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... Eluted proteins were precipitated the supernatant by addition of ammonium sulfate to 67% saturation and dissolved in buffer D and loaded on equilibrated 5 mL HiTrap Heparin HP sepharose column (GE Healthcare). The protein was eluted with a linear 0-1 M NaCl gradient in buffer D ...
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bioRxiv - Microbiology 2023Quote: ... centrifuged 30 min at 2150g and concentrated/diafiltrated against loading buffer (D-PBS, 500 mM NaCl, 5 mM Imidazole) using the AKTA crossflow system (GE healthcare) supplemented with a 10KDa ...
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bioRxiv - Immunology 2023Quote: ... The proteins were induced in Escherichia coli BL21 (Gold) cells with 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) for 5 hour and purified by glutathione sepharose (GE healthcare) followed by on-column TEV cleavage and finally by size-exclusion chromatography on Hiload 16/60 Superdex 75 coloumn (GE healthcare).
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bioRxiv - Neuroscience 2021Quote: ... placed in 5% milk PBST plus 1:2500 mouse specific HRP antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Biophysics 2024Quote: ... The labeled antibodies were then purified using a NAP-5 column (17085302, GE Healthcare) and determined by a UV-Vis spectrophotometer.
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bioRxiv - Neuroscience 2020Quote: ... 150 mM NaCl) containing 5% skim milk (Meiji) and subjected to primary antibody for 2 h and HRP-conjugated secondary antibody (GE Healthcare) for 30 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell were washed with phosphate-buffered saline (PBS, GE Life Sciences), scrape harvested ...
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bioRxiv - Neuroscience 2020Quote: ... Antibodies were then purified using size exclusion columns (Illustra NAP-5 columns, #17085302, GE Healthcare). The absorption of IgG at 280 nm and Cy3B dye at 559 nm of the reaction products was measured by spectrophotometry (NanoDrop ND-1000 Spectrophotometer) ...
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bioRxiv - Cell Biology 2023Quote: ... Phosphate-buffered saline (PBS) was purchased from Hyclone (GE Healthcare Life Sciences). Calf thymus DNA solution was purchased from ThermoFisher.
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bioRxiv - Biochemistry 2024Quote: ... Antibodies were separated from unincorporated fluorophores on desalting columns (NAP-5 or PD-10, GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... Mg++ free phosphate buffered saline (PBS) and layered over Ficoll-Pacque (GE Healthcare), centrifuged at 2,000 rpm with no brake for 20 minutes at room temperature (RT ...
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bioRxiv - Neuroscience 2020Quote: ... Binding was detected with an anti-mouse Cy-3 and anti-rabbit Cy-5 antibodies (GE Healthcare). Blots were scanned using a Typhoon 5 (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... Supernatant was harvested after 4-5 days and antibody purified on Protein G Sepharose (Abcam, GE Healthcare) following standard protocols ...
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bioRxiv - Immunology 2021Quote: ... 2-D and 3-D images were obtained and analyzed using the software program MicroView (GE Healthcare).
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bioRxiv - Developmental Biology 2021Quote: ... and resuspended in Dulbecco’s Phosphate-Buffered Saline (GE Healthcare Life Sciences, Logan, Utah, USA), centrifuged and resuspended in fresh resuspension solution ...
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bioRxiv - Immunology 2021Quote: ... was extensively dialyzed into phosphate-buffered saline (PBS, pH 7.2) (GE Life Sciences, PA) using the Slide-A-Lyzer Dialysis Cassettes (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: We used a modified version of a previously described protocol (41). Briefly, we immobilized monoclonal 1D4 antibody (generously provided by D. Oprian, Brandeis University) onto Sepharose-4B (GE Healthcare Life Sciences). All steps were done in a darkroom under dim red-light illumination ...
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bioRxiv - Neuroscience 2021Quote: ... ATXN1 blots were then placed in 5% milk PBST plus 1:2500 rabbit specific HRP antibodies (GE Healthcare) while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... the medium was loaded overnight onto a ~5 ml anti-CD9 AR40A746.2.3 affinity column (7.5 mg antibody/ml CNBr Activated Sepharose 4B beads; GE Healthcare) in line of an AKTA explorer (GE) ...
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bioRxiv - Neuroscience 2023Quote: ... ATXN1 blots were then placed in 5% milk PBST plus 1:2500 rabbit specific HRP antibodies (GE Healthcare) while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... incubated for 40 min at 4°C with nickel phosphate loaded Sepharose beads (GE Healthcare) to separate the His tag ...
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bioRxiv - Neuroscience 2021Quote: ... while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Plant Biology 2020Quote: ... The extracts were incubated with 5 μL anti-HA antibodies coupled with 30 μL Protein-A Sepharose (GE Healthcare) overnight at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Microbiology 2024Quote: ... BMDMs were detached using cold phosphate-buffered saline (PBS) (Hyclone, GE Healthcare Inc. South Logan, UT), the cell viability and concentration was adjusted using a hemacytometer as previously described102 diluting 1 part of 0.4% trypan blue (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... the siRNA D-023324-05 (GE Healthcare Dharmacon) and Stealth RNAis (#HSS126645 ...
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bioRxiv - Neuroscience 2020Quote: ... the 2-D Clean-Up Kit (GE Healthcare) was used following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The probe (L3-12-D, GE Healthcare Japan) was applied to the target site with the upper surface of the echo image facing the knee joint and the lower surface facing the ankle joint ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated with secondary antibodies conjugated with horseradish peroxidase (HRP) diluted in 5% skim milk in TBS-T (1:10,000, GE healthcare). Labelled proteins were detected via enhanced chemiluminescence (Pierce ECL Western Blotting Substrate ...
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bioRxiv - Cancer Biology 2021Quote: Approximately 500,000 cells were transfected using a final concentration of 20 nM EPSTI1 (J-015094-09-0020 and J-015094-12-0020) or non-targeting (D-001810-01-20 and D-001810-02-20) ON-TARGETplus siRNAs from GE Healthcare Dharmacon using 20 µL of Lipofectamine RNAiMAX (ThermoFisher #13778-150 ...
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bioRxiv - Cell Biology 2022Quote: ... Cat# 015094-12-0020) or non-targeting (D-001810-01-20 and D-001810-02-20) ON-TARGETplus siRNAs from GE Healthcare Dharmacon using 20 μL of Lipofectamine RNAiMAX (ThermoFisher #13778-150 ...
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bioRxiv - Microbiology 2023Quote: ... 17µl (∼ 5 µg) of each Cy-5-labelled (GE Healthcare) cDNA of the sample and an equal amount of Cy-3-labelled (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... Sterile and filtered phosphate buffered saline (PBS, 1X) was obtained from GE Life Sciences (Pittsburgh, PA, USA). Unless otherwise noted ...
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bioRxiv - Neuroscience 2021Quote: ... Sterile and filtered phosphate buffered saline (PBS, 1X) was obtained from GE Life Sciences (Pittsburgh, PA, USA). Unless otherwise noted ...
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bioRxiv - Evolutionary Biology 2024Quote: ... BMDM monolayers were detached using cold phosphate-buffered saline (PBS) (Hyclone, GE Healthcare Inc. South Logan, UT) and cultured ...
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bioRxiv - Microbiology 2023Quote: ... BMDM monolayers were detached using cold phosphate-buffered saline (PBS) (Hyclone, GE Healthcare Inc. South Logan, UT) and cultured ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times with PBST and then placed in 5% milk PBST plus 1:2500 rabbit specific horseradish peroxidase (HRP) antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were then washed with TBS-T (3x, 5 min each) and incubated with horse radish peroxidase (HRP)-conjugated secondary antibodies (GE Healthcare). Following another wash step in TBS-T ...
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bioRxiv - Immunology 2022Quote: ... Membranes were then incubated with the corresponding HRP-tagged secondary antibodies in BSA (5% w/v) PBS-T for 1 hour before visualisation using Amersham ECL prime detection reagent (GE healthcare) and a G:Box Chemi XX6 system (Syngene) ...
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bioRxiv - Molecular Biology 2023Quote: ... the membranes were probed with ECL Plex Cy3- or Cy5-conjugated goat anti-mouse or anti-rabbit secondary antibodies (Cytiva) and scanned on an Amersham Typhoon 5 Phosphorimager (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... D) were deconvolved using Softworx (Applied Precision; Rača, Slowakia). Images acquired from LSM 710 confocal microscope were deconvolved using Huygens Software (Scientific Volume Imaging ...
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bioRxiv - Biochemistry 2022Quote: ... 5 % glycerol into two 5 mL MBP-trap cartridges (GE Healthcare) connected in tandem before removal of the Ni-NTA cartridges ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were mixed 1:1 with phosphate-buffered saline (PBS) and layered on Ficoll–Paque (GE Healthcare; 1714402). Cells were spun at 800 g for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... gingivalis were resuspended in Hyclone Dulbecco’s phosphate[buffered saline (DPBS) containing calcium and magnesium (GE Healthcare, Pittsburgh, PA), and were quantified using a Klett[Summerson photometer (Bel-Art ...