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Citations for GE Life Sciences :
151 - 168 of 168 citations for Cytomegalovirus gH Pentamer Complex since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The mixed complexes were incubated over night at 4 °C and the formed R2SP complex was separated from excess SPAG1/PIH1D2 using a Superose 6 16/60 XK (GE Healthcare), see Supplementary Information for detailed protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The following morning unbound G protein and Nb35 were separated from the complex by SEC on a Superdex 200 10/300 GL column (GE Healthcare) pre-equilibrated with the buffer containing 20 mM HEPES/KOH pH7.5 ...
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bioRxiv - Biochemistry 2023Quote: SUV420H1-H2A.Z nucleosome complex was assembled in a ratio of 3:1 and was purified on HiLoad Superdex 200 10/300 (GE Healthcare) size-exclusion liquid chromatography column (Buffer ...
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bioRxiv - Biophysics 2023Quote: ... The 35S-methionine and 35S-cysteine incorporation into the core- and complex-glycosylated CFTR variants were visualized by fluorography and quantified using phosphorimage analysis on a Typhoon imaging platform (GE Healthcare). CFTR radioactivity was quantified after background subtraction ...
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bioRxiv - Biochemistry 2024Quote: apoGAD65 and b96.11 Fab were purified as described and complex formation performed by mixing freshly purified GAD65 with 2X molar excess of b96.11 followed by incubation on ice for 15 min before subjecting the complex to analytical size exclusion chromatography using a Superdex 200 Increase 10/300 GL (GE Healthcare). The sample was used as is for negative stain EM ...
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bioRxiv - Biochemistry 2024Quote: ... The complex was separated from Nb excess by size exclusion chromatography (SEC) on a Superdex-200 10/300 column (GE Healthcare). To concentrate we used protein concentrators with a cut-off of 100 kDa (Amicon ...
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bioRxiv - Microbiology 2024Quote: ... The eluted Cas1-Cas2/3 complex was concentrated and further purified by gel filtration chromatography (Superdex200 10/300 GL, GE Healthcare) in buffer C ...
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bioRxiv - Microbiology 2019Quote: ... The concentrated protein complex was further purified using size-exclusion chromatography with a Superose-6 Increase 10/300-GL (GE Life Sciences) column equilibrated in Buffer B (50 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... Yield can be improved by taking pooled TRAPP complex before SEC and manually running protein through a 1 mL HisTrap™ FF column (GE Healthcare) that had been equilibrated in NiNTA A buffer followed by buffer exchange into SEC buffer using a 5 mL HiTrap® Desalting Column (GE Healthcare) ...
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bioRxiv - Biochemistry 2019Quote: ... 100 μL of purified protein/complex at 2-5 mg/mL was injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in a buffer containing 20 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The complex formed in the 1:2.5 mixture was purified on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) equilibrated in assay buffer and run at room temperature ...
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bioRxiv - Immunology 2024Quote: ... was incubated at room temperature for 20 minutes with a slight molar excess of c13G8 Fab and the complex was purified by SEC on a HiLoad 16/600 Superdex 200 column (GE Healthcare Biosciences) in 2 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2024Quote: ... was incubated at room temperature for 20 minutes with a 1.2-fold molar excess of ADI-46143 Fab and the complex was purified by SEC on a Superdex 200 Increase 10/300 GL (GE Healthcare Biosciences) in 2 mM Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2019Quote: ... Flow through containing the unbound ID2 complex was diluted to 150 mM NaCl concentration and loaded onto a HiTrap Heparin HP affinity column (GE Healthcare Life Sciences). Using a shallow NaCl gradient ...
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bioRxiv - Neuroscience 2022Quote: ... The stabilized Q-SNARE complex was purified by Ni2+-NTA affinity chromatography followed by ion-exchange chromatography on a Mono Q column (GE Healthcare, Piscataway, NJ) in the presence of 50 mM n-octyl-β-D-glucoside (OG)10 ...
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bioRxiv - Biochemistry 2023Quote: ... The TEV protease cleaved PIK3CB/ybbr-PIK3R1 complex was separated on a Superdex 200 size exclusion column (GE Healthcare, Cat# 17-5174-01) equilibrated with 20 mM Tris [pH 8.0] ...
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bioRxiv - Immunology 2023Quote: ... The EROS-NOX2-p22phox-7G5 Fab complex was concentrated and applied onto a Superose 6 Increase 10/300 GL column (GE HealthCare, Chicago, IL) in Buffer D (20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... and Rgl2RA were mixed in the GF buffer and the complex was purified on SEC using a gel-filtration column (GE Healthcare, HiLoad Superdex 75). The peak fractions containing both proteins in the 1:1 ratio were collected and concentrated to set up crystallization screenings ...