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Citations for GE Life Sciences :
401 - 450 of 976 citations for Crimean Congo Haemorrhagic Fever virus CCHFV glycoprotein C Gc sheep Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... The bacterial protein PA1217 was fused to a GST-tag by cloning it into a pGEX-6P-1 vector (GE Healthcare, Chicago, IL) using the BamHI and EcoRI restriction sites (Thermo Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... The cleaved protein was purified away from SenP1 and the N-His6-SUMO-tag by FPLC on a Mono Q 10/100 GL anion exchange column (GE Healthcare Life Sciences) using buffers containing 50 mM EPPS (pH 8.5) ...
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bioRxiv - Immunology 2020Quote: ... and the C-terminal recombinant proteins containing a N-terminal 6xHis-tag were purified from the resulting supernatant using Hitrap Ni-NTA column (GE Healthcare, Uppsala, Sweden) according to the manufacturer instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was subjected to TEV cleavage to remove the GST tag and then further purified using a Mono Q 5/50 GL anion exchange column (GE Healthcare Life Sciences). Finally ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then collected by vacuum filtration onto glass microfiber filters (grade GF/C, 125 mm in diameter, pre-combusted at 460°C for > 5 h, GE Healthcare Whatman). The filters were transferred to 50 mL centrifuge tubes (Sarstedt ...
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bioRxiv - Cell Biology 2022Quote: ... Extract was cleared by centrifugation at 186.000 x g for 1 h at 4°C and then incubated at 4°C with Glutathione Sepharose™ 4B resin (GE Healthcare) for 3h ...
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bioRxiv - Cell Biology 2020Quote: ... 28°C and purified on HisTrap columns (GE Healthcare) by Imidazole gradient elution on AKTA Prime according to manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 4°C onto 0.2uM PVDF membranes (GE Healthcare Biosciences). After transfer ...
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bioRxiv - Genomics 2024Quote: ... transferred onto a Hybond-C nitrocellulose membrane (GE Healthcare), then blocked with TBST containing 5% (w/v ...
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bioRxiv - Immunology 2019Quote: ... followed by horseradish peroxidase (HRP)-labeled secondary antibody goat-anti-mouse IgG Fc (Pierce Antibodies) or donkey-anti-rabbit IgG (GE Life Sciences). HRP conjugates bound to antigen were detected and visualized by using an ECL detection kit (GE Life Sciences).
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bioRxiv - Immunology 2022Quote: ... followed by horseradish peroxidase (HRP)-labeled secondary antibody goat-anti-mouse IgG Fc (Product #: 31433) (Pierce Antibodies) or donkey-anti-rabbit IgG (Product #: NA934V) (GE Life Sciences). HRP conjugates bound to antigen were detected and visualized by using an ECL detection kit (GE Life Sciences).
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bioRxiv - Immunology 2019Quote: ... followed by horseradish peroxidase (HRP)-labelled secondary antibody goat-anti mouse IgG Fc (Pierce Antibodies) or donkey-anti-rabbit IgG (GE Life Sciences). HRP conjugates bound to antigen were detected and visualized by using an ECL detection kit (GE Life Sciences).
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bioRxiv - Molecular Biology 2019Quote: ... fused to triple epitope tags were prepared by directionally cloning in-frame epitope tags into the BamHI and EcoRI restriction sites of pGEX-4T-1 (GE Healthcare Life Sciences #28954549). PCR amplicons were prepared from a PAGE-purified oligonucleotide template with forward primers containing a BglII restriction site and reverse primers containing HindIII–BamHI–EcoRI sites (Supplementary Table S1) ...
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bioRxiv - Molecular Biology 2020Quote: ... 4.5 µL of Cy5 Mono-Reactive NHS Ester Fluorescent Dye (ChIP samples) or Cy3 Mono-Reactive NHS Ester Fluorescent Dye (control samples Input DNA or no-tag ChIP) (GE Healthcare, PA25001 and PA23001) were added ...
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bioRxiv - Neuroscience 2023Quote: ... The remaining tag and protease were removed by reverse Ni-NTA purification in batch mode (Ni-Sepharose High Performance; GE Healthcare, 17-5268-02). Final polishing of the target proteins was done through size exclusion on a HiLoad 26/600 Superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and eluted with a linear gradient of imidazole (0–1,000 mM) in His-tag-binding buffer using the ÄKTA FPLC™ Fast Protein Liquid Chromatography system (GE Healthcare, Princeton, NJ, USA). The eluted protein peak was collected and dialyzed against buffer D containing 50 mM Tris–HCl (pH 8.0) ...
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bioRxiv - Microbiology 2019Quote: ... Blots were UV cross-linked and baked at 80°C for 2 h prior to pre-hybridization at 42°C for 1 h in Rabid-Hyb Buffer (GE Healthcare Life Sciences). Hybridization with α-32P-ATP end-labeled DNA oligo probes was performed at 42°C for 2-16 h ...
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bioRxiv - Microbiology 2020Quote: Binding kinetics and affinities for ACE2.Fc were assessed using surface plasmon resonance technology on a Biacore T200 instrument (GE Healthcare, Marlborough, MA) using a Series S CM5 sensor chip in filtered and degassed HBS-EP running buffer (10 mM HEPES ...
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bioRxiv - Microbiology 2022Quote: ... were collected by vacuum filtration onto glass microfiber filters (grade GF/C, 47 mm in diameter, pre-combusted at 460°C for >8 h, GE Healthcare Whatman, Buckinghamshire, UK), immediately plunged into liquid nitrogen ...
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bioRxiv - Biophysics 2020Quote: ... thrombin cleavage of the His6-tag overnight and final ion exchange chromatography with a MonoQ or MonoS column (Äkta purifying system, GE Healthcare, Little Chalfont, United Kingdom). Synaptobrevin 2 49-96 S79C was labeled with Atto488 maleimide ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatants were filtrated and subjected to affinity chromatography using a 5 mL HisTrap HP His-tag purification column (GE healthcare, cat. # GE29-0510-21) operated by a peristaltic pump (Cytiva ...
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bioRxiv - Microbiology 2020Quote: ... the removal of SUMO protease and SUMO tag from the cleavage reaction was performed with affinity chromatography on a HisTrap HP column (GE Healthcare, Code#:17-5248-01). The SUMO protease and SUMO tag will stay in the HisTrap HP column and the cleaved native protein will be in the flow-through fractions ...
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bioRxiv - Biochemistry 2022Quote: ... The cDNAs of full-length TPR-CHAT were synthesized using the bacterial expression vector pGEX-6P-1 (GE Healthcare, with an N-terminal GST tag). Point mutations were introduced using Fast Site-Directed Mutagenesis Kit in the Sb-gRAMP gene and verified by DNA sequencing ...
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bioRxiv - Biophysics 2021Quote: ... at 4°C using AKTA Pure HPLC system (GE Healthcare). The column was washed with 20 mM NaPi (pH 8.0 ...
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bioRxiv - Neuroscience 2020Quote: ... Gels were transferred onto Hybond-C nitrocellulose membrane (GE Healthcare). Membranes were blocked in 4% milk for 1 hour and incubated overnight at 4°C with shaking in the appropriate primary antibody against Nischarin (1:500 ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were transferred to Nitrocellulose membrane (Hybond-C, GE healthcare) in transfer buffer (50 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were transferred to Nitrocellulose membrane (Hybond-C, GE healthcare) in transfer buffer using a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... 20°C without break in a 30% Percoll (GE Healthcare) solution ...
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bioRxiv - Molecular Biology 2021Quote: ... Hybond-C Extra membrane (RPN203E) was purchased from GE healthcare. ECL Prime (RPN2236 ...
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bioRxiv - Cell Biology 2020Quote: ... at 37°C using a Deltavision core microscope (Applied Precision) equipped with a CoolSnap HQ2 CCD camera ...
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bioRxiv - Physiology 2023Quote: ... transferred to a nitrocellulose membrane Hybond-C Extra (GE Healthcare) in transfer medium (0.025MTris ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were transferred to Hybond C-Extra nitrocellulose filters (GE Healthcare). Primary antibodies used for immunoblot analysis included ...
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bioRxiv - Bioengineering 2024Quote: ... at 4 °C using an ÄKTA Pure 25 M (GE Healthcare). The column was washed with 75 mM imidazole [in 20 mM NaH2PO4 and 0.5 M NaCl (pH 7.4)] ...
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bioRxiv - Molecular Biology 2021Quote: ... The secondary antibody only negative controls established gating for each sample and NeuN labelled nuclei were then sorted and collected in tubes pre-coated with foetal calf serum (FCS; #SH30084.02, Hyclone, GE Healthcare Life Sciences, South Logan, Utah, USA). Purity checks were performed and all samples were validated at >97% purity.
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bioRxiv - Molecular Biology 2019Quote: Purification was performed at 4 °C using His GraviTrap columns (GE Healthcare). Equilibration was done with 10 ml of B-PER II before extracted soluble fractions were applied to the column ...
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bioRxiv - Immunology 2020Quote: ... at 25 °C using a Series S Sensor Chip NTA (GE Healthcare). CSP27 and CSP9 were immobilized on separate channels on the sensor chip surface as per the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2019Quote: ... using a DeltaVision climate control unit (37°C, 5% CO2) (Applied Precision). The kinetics of pre-stress followed by treatment was analysed from images captured at 2 min intervals for 100 min ...
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bioRxiv - Synthetic Biology 2021Quote: ... the cultures were applied to CM SephadexTM C-25 column (GE Healthcare) equilibrated with distilled water ...
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bioRxiv - Plant Biology 2020Quote: ... Hybridizations were performed at 65°C in Rapid-Hyb buffer (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: SPR experiments were performed at 25°C using Biacore 3000 (GE healthcare) or Biacore T200 (Cytiva) ...
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bioRxiv - Biophysics 2022Quote: ... cells at 32°C with 0.4 mM Isopropyl-D-thiogalactoside (GE Healthcare) for 8 h ...
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bioRxiv - Cell Biology 2023Quote: Images were acquired at 30°C using a Deltavision Elite (Applied Precision) microscope equipped with an Olympus UPlanSApo 60x or 100x (NA1.4 ...
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bioRxiv - Microbiology 2022Quote: ... rolling at 4°C with 100 μl of Ni-NTA Agarose (GE Healthcare) pre-equilibrated with the SEC buffer ...
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bioRxiv - Immunology 2021Quote: ... C monoclonal antibody (W6/32) coupled Sepharose-4B resin (GE Healthcare, IL, USA) at room temperature for 2 hours ...
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bioRxiv - Molecular Biology 2019Quote: ... Cas3/I-C was further loaded onto HiTrap Heparin HP column (GE Healthcare) to remove impurities ...
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bioRxiv - Immunology 2019Quote: ... 94°C 5min) with 6 µl of RNA and random hexamers (GE Healthcare). A PCR was then performed based on an established protocol (Tiller et al. ...
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bioRxiv - Biochemistry 2021Quote: Purification was performed at 4°C using an ÄKTA™ pure (GE Healthcare) to control flow and column pressure ...
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bioRxiv - Cell Biology 2020Quote: ... followed by transfer onto a Hybond–C Extra Nitrocellulose membrane (GE1060000, GE Healthcare) for 105 min at 100 V ...
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bioRxiv - Biochemistry 2021Quote: ... 0.005% P20 surfactant) at 25 °C using a Biacore 3000 instrument (GE Healthcare). For measurements involving ATP/MgCl2 ...
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bioRxiv - Developmental Biology 2021Quote: ... were coupled overnight at 4°C to protein A Sepharose beads (GE Healthcare). Coupled beads were washed three times in EB buffer (50 mM Hepes ...