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Citations for GE Life Sciences :
151 - 200 of 759 citations for Cow Serine protease HTRA2 mitochondrial HTRA2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... the 6x His tag was cut off by the TEV protease and the protein was further purified by size-exclusion chromatography on a Superdex 75 HiLoad 26/60 column (GE Healthcare) in SEC buffer (20 mM Na2HPO4 ...
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bioRxiv - Biophysics 2019Quote: ... and protein was released from the resin by PreScission Protease (PPX) action at 4 °C overnight and further purified on a Superdex 200 column (GE Healthcare) equilibrated in SEC buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... Both the cleaved His-tag and the His-tagged protease were removed from protein solutions by passage over loose nickel beads (GE Healthcare). The tag-free NDC-NDC was further purified with HiTrap SP HP ion exchange column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM DTT in the presence of TEV protease and subsequently purified using a 5 mL HiTrap HP Heparin column (GE Healthcare), eluting with a buffer containing 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... with the addition of 1 mg of 3C protease per 3 L of culture volume and further separated on a Superdex 200 HiLoad column (GE Healthcare) in 50mM Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted proteins were digested at 4°C with specific proteases and further purified by ion-exchange chromatography using a HiTrap SP HP or monoQ (GE Healthcare) columns and a gradient running from 0% buffer A (40 mM TRIS ...
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bioRxiv - Immunology 2020Quote: ... and thereafter were eluted with 20 mM reduced glutathione (recombinant GST or GST-fusion peptides) or incubated with PreScission Protease (GE Healthcare) overnight at 4 °C to remove the GST tag ...
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bioRxiv - Cell Biology 2021Quote: ... The Trx-6xHis-3C tag was removed by protease 3C cleavage and the untagged protein was further purified by size-exclusion chromatography (Superdex 75, GE Healthcare).
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bioRxiv - Genomics 2021Quote: ... we used the Ascaris proteins cleaved from GST-fusions bound to Glutathione Sepharose 4B by treatment with PreScission protease (GE Healthcare). AsWAGO-2 peptides (YASRRMFELTKQSRDDYRA and RNGGQETSSSSSGEAGHLDI ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleaved MBP tag and TEV protease were removed by anion exchange chromatography using a Mono Q 5/50GL (GE Healthcare) column pre-equilibrated with buffer-B ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cleaved protein was separated from TEV protease by a subtractive Ni-NTA pass and loaded on to a HiLoad 16/60 Superdex 75 column (GE Healthcare) equilibrated with 20 mM Hepes ...
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bioRxiv - Biochemistry 2023Quote: ... the His-MBP and His tags were removed by TEV protease and a passage through a Ni2+chelating Sepharose (GE Healthcare). The final purification step was size-exclusion chromatography on Superdex 200 size exclusion chromatography (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... The GST tag was cleaved off by incubating the washed beads in wash buffer supplemented with PreScission Protease (∼100 mL at 76 mg/mL) (GE Healthcare) overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... the His-MBP tag was cleaved off by TEV protease and the protein was further purified by a passage through a Ni2+-Chelating Sepharose (GE Healthcare). IHO1 was then purified by a gel filtration on Superdex 200 (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... followed by removal of the N-terminal His6-Ztag by TEV protease cleavage and size exclusion chromatography using a Superdex 200 16/60 column (GE Healthcare) (Roostalu et al ...
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bioRxiv - Biochemistry 2023Quote: ... The liberated affinity-tag and the His-tagged SENP1 and 3C proteases were removed by a second round of affinity purification with HisTrap FF columns (GE Healthcare). All proteins were purified with a final size exclusion chromatography (HiLoad 16/600 Superdex 75pg ...
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bioRxiv - Immunology 2023Quote: ... Affinity-purified proteins were incubated with HRV-3C protease to remove purification tags and subsequently purified by size-exclusion-chromatography on a Superose 6 Increase column (GE Healthcare) in TBS
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bioRxiv - Biochemistry 2023Quote: ... the N-terminal His tag was cleaved by overnight dialysis with TEV protease at 4 °C and purified by size exclusion chromatography (SEC) (HiLoad Superdex-200 16/600 column, GE Healthcare) in CHIP storage buffer (50 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... the His6-NusA-tag was removed using the HRV3C protease and was further purified by SEC on Superdex 75 Increase 10/300 (GE Healthcare) column in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... Eluted protein was digested with Ulp1 protease at 4°C for 2 h and then further purified on a Heparin HP column (GE Healthcare), eluting with a linear gradient of increasing NaCl concentration from 300 mM to 1 M in 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... Eluted protein was digested with Ulp1 protease at 4°C for 2 h and then further purified on a Heparin HP column (GE Healthcare), eluting with buffer containing 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was digested with the TEV protease overnight and further purified by gel filtration chromatography using a Superose 6 column (GE Healthcare), eluted in buffer C (25 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... The N-terminal GFP-His10 tag was cleaved overnight with 3C protease before gel filtration chromatography by using a Sepharose 6 Increase 10/300 GL column (GE Healthcare). For the purification of apo proteins ...
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bioRxiv - Plant Biology 2024Quote: ... the untagged protein was separated from cleaved tags and the 6xHis-tagged 3C protease by affinity chromatography using 5 ml HisTrap HP NTA column (GE Healthcare). If proteins were also tagged with MBP ...
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bioRxiv - Biophysics 2024Quote: ... His6-GB1-tag was subsequently cleaved by TEV protease (3 mM) and separated from bZIP on a cation exchange column (HiTrap SP; GE Healthcare) using a 0–2 M gradient of sodium chloride ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted protein was cleaved with Precission™ protease for 2 hours at room temperature and applied to a 5 mL HiTrap Q HP column (GE Healthcare). Protein was eluted from the Q HP column with a 2M NaCl buffer in a 100 mL gradient ...
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bioRxiv - Molecular Biology 2021Quote: ... hRpn13 Pru was eluted from the resin by overnight incubation with 50 units per mL of PreScission protease (GE Healthcare Life Sciences) in buffer B (20 mM sodium phosphate ...
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bioRxiv - Molecular Biology 2022Quote: ... The fusion protein was cleaved with 10% (w/w) TEV protease overnight at 4°C and the mixture was loaded on a 5 ml HisTrap™FF column (GE Healthcare). 6xHis-tag-labeled disulfide bond isomerase and TEV protease were retained ...
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bioRxiv - Systems Biology 2021Quote: ... The Ni-elution was cleaved with TEV protease and the PDZ-ANXA2 was purified by cation exchange on a HiTrap SP HP column (GE Healthcare, Chicago, Illinois). Proteins were aliquoted at 5-7 mg/ml concentration before flash freezing in LN2 ...
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bioRxiv - Cell Biology 2022Quote: The real time protein-protein interaction of protease and PDZ domain proteins of PfHtrA2was analysed by SPR using Biacore T200 instrument (GE Healthcare, Uppsala, Sweden) as described previously [7] ...
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bioRxiv - Microbiology 2021Quote: ... Cleavage of the GST tag to induce cleaved fusion proteins was performed using PreScission protease (10 U/mg of fusion protein, GE Healthcare, Uppsala, Sweden) or thrombin protease (10 U/mg of fusion protein ...
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bioRxiv - Biochemistry 2023Quote: ... The TEV protease cleaved PIK3CB/ybbr-PIK3R1 complex was separated on a Superdex 200 size exclusion column (GE Healthcare, Cat# 17-5174-01) equilibrated with 20 mM Tris [pH 8.0] ...
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bioRxiv - Evolutionary Biology 2021Quote: ... or with a GFX PCR DNA kit and Gel Band Purification kit (GE Healthcare, USA) following manufacturer guidelines ...
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bioRxiv - Neuroscience 2023Quote: ... The remaining tag and protease were removed by reverse Ni-NTA purification in batch mode (Ni-Sepharose High Performance; GE Healthcare, 17-5268-02). Final polishing of the target proteins was done through size exclusion on a HiLoad 26/600 Superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... Importin-α and Ran proteins lacking GST were prepared by cleavage with PreScission protease (20 U/mg of fusion protein; GE Healthcare, Uppsala, Sweden) at 4 °C for 12 h ...
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bioRxiv - Biophysics 2019Quote: A pET-based plasmid overexpressing each subunit of RNAP (full-length α, β, ω) as well as β’-PPX-His10 (PPX; PreScission protease site, LEVLFQGP, GE Healthcare) was co-transformed with a pACYCDuet-1 plasmid containing Eco rpoZ into Eco BL21(DE3) ...
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bioRxiv - Microbiology 2020Quote: ... the removal of SUMO protease and SUMO tag from the cleavage reaction was performed with affinity chromatography on a HisTrap HP column (GE Healthcare, Code#:17-5248-01). The SUMO protease and SUMO tag will stay in the HisTrap HP column and the cleaved native protein will be in the flow-through fractions ...
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bioRxiv - Immunology 2023Quote: ... Eluate was then cleaved with TEV protease overnight at 4 °C and subsequently purified further by size exclusion chromatography (Superdex 200 26/600 from GE Healthcare; cat # GE28-9893-36) in TBS ...
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bioRxiv - Cell Biology 2020Quote: ... GST kit (GE Healthcare, Chicago, IL) was used for purifying GST-Rheb following manufactures instructions and eluted with reduced glutathione ...
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bioRxiv - Cell Biology 2019Quote: ... ECL chemiluminescent kit (RPN2132, GE Healthcare) was used for detection according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... A His-capture kit (GE Healthcare) was used to immobilize anti-His antibodies on CM5 chips (as recommended by the manufacturer) ...
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bioRxiv - Cancer Biology 2019Quote: ... The His SpinTrap Kit (GE Healthcare) was used according to manufacturer’s recommendations to purify the recombinant CELF1 proteins ...
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bioRxiv - Cell Biology 2019Quote: ... The ECL Prime Kit (GE Healthcare) was used for HRP detection.
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bioRxiv - Microbiology 2022Quote: ... The DNA was extracted using the kit Illustrative Bacteria Genomic Mini Spin Kit (GE Life Sciences, Switzerland) according to manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2020Quote: ... using the amine coupling kit (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... with an ECL-plus kit (GE Healthcare). The intensity of the bands was analyzed by NIH Image J (http://rsbweb.nih.gov/ij/).
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bioRxiv - Cell Biology 2022Quote: ... Cy5-labelling kit purchased from GE Healthcare. Para-aminoblebbistatin (ax-494682 ...
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bioRxiv - Cell Biology 2022Quote: Cy5-labelling kit purchased from GE Healthcare was used for conjugation of human fibronectin (Corning) ...
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bioRxiv - Microbiology 2021Quote: ... and molecular biology kits from GE Healthcare and Promega ...
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bioRxiv - Immunology 2020Quote: ... and Amine coupling kit (GE Healthcare, USA) were used for immobilization of SARS-CoV-2 Spike S1+S2 ECD-His recombinant protein (Sino Biological ...