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Citations for GE Life Sciences :
1 - 50 of 650 citations for Cow Glycerol kinase GK ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 50% glycerol (GE Healthcare) and ρ-phenylenediamine 0.1% (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... 10% glycerol using PD-10 columns (GE healthcare). If required YpfP was concentrated using a Vivaspin 20 30MWCO until 2.4 mg/ml ...
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bioRxiv - Microbiology 2020Quote: ... 10% glycerol) using PD-10 desalting columns (GE Healthcare). Buffer exchanged protein was stored in aliquots at -80°C.
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bioRxiv - Biochemistry 2023Quote: ... 5 % glycerol on an ÄKTA Pure (GE Life Sciences). Where nucleotide was present ...
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bioRxiv - Cancer Biology 2023Quote: The cell proliferation rate was evaluated using the Kit Biotrak ELISA System (GE Healthcare, Orsay, France) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... Glycerol was continually replaced using a peristaltic pump (GE HealthCare) with a flow rate of one chamber volume per hour ...
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bioRxiv - Biochemistry 2019Quote: ... 5% Glycerol) 120 mL Superdex200 16/600 column (GE Healthcare). 2 mL fractions were collected and analyzed on a 15% SDS-PAGE gel ...
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bioRxiv - Developmental Biology 2021Quote: ... 5% glycerol) Superdex 75 10/300 GL column (GE Healthcare) for size exclusion chromatography ...
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bioRxiv - Biochemistry 2022Quote: ... 5% glycerol and loaded onto a MonoQ column (GE Healthcare) preequilibrated with the MonoQ buffer ...
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bioRxiv - Systems Biology 2022Quote: ... the coding sequence for this kinase (GE Healthcare Dharmacon: MHS6278-202756930) and its binding partner TAB1 (GE Healthcare Dharmacon ...
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bioRxiv - Molecular Biology 2021Quote: ... and 5% glycerol and loaded onto a Heparin column (GE Healthcare), eluted with a linear NaCl gradient (0.1 to 1M) ...
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bioRxiv - Biochemistry 2020Quote: ... 5% (v/v) glycerol using a HiTrap Desalting column (GE Healthcare). All protein samples were aliquoted and stored at −80 °C.
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bioRxiv - Biochemistry 2022Quote: ... 5 % glycerol into two 5 mL MBP-trap cartridges (GE Healthcare) connected in tandem before removal of the Ni-NTA cartridges ...
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bioRxiv - Biochemistry 2020Quote: ... and 10% Glycerol using a Superdex S200 16/60 (GE Healthcare) or Superdex S75 16/60 (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... and 10% glycerol) on a PD-10 column (GE Healthcare, 17085101), concentrated with a 10 kDa MWCO Amicon Ultra-4 device (Millipore ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1 mM DTT) with 10% glycerol using PD10 columns (GE Healthcare), followed by further concentration using an Amicon Ultra 30K device (Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... The kinase reaction was concentrated and run over Superdex-75 (GE Healthcare) to remove kinases and ATP and phosphorylation of the proteins were confirmed by electrospray mass spectrometry using a Sciex X500B QTOF system.
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bioRxiv - Cell Biology 2019Quote: ... 20% w/v Glycerol using a NAP5-column (GE Healthcare, Freiburg, Germany), partitioned in 10 µl aliquots and stored at -20°C for a maximum of 2 weeks.
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bioRxiv - Microbiology 2019Quote: ... 10% glycerol] was performed by PD10 desalting column (GE Healthcare Buckinghamshire, UK), as per manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... and 5% glycerol) and run through a tandem MBPTrap column (GE Healthcare) and HiTrap heparin HP column (GE Healthcare ...
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bioRxiv - Molecular Biology 2022Quote: ... 5% glycerol) using PD SpinTrap G-25 columns (GE Healthcare #28-9180-04) pre-equilibrated with conjugation buffer ...
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bioRxiv - Biochemistry 2023Quote: ... 10% (v/v) glycerol) and loaded onto a heparin affinity column (GE healthcare) and eluted with 100% buffer D (buffer C + 1.5 M NaCl) ...
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bioRxiv - Cancer Biology 2022Quote: ... 10% w/v glycerol and applied to a MonoS or Capto S column (GE Healthcare). Protein was eluted with a gradient to 1.0 M NaCl ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... 10% glycerol) and was purified using a Superdex 75 size exclusion chromatography column (GE Healthcare). Ub mutants K63R and K48R are from Boston Biochem.
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bioRxiv - Cell Biology 2021Quote: ... and the phosphorylation levels of receptor tyrosine kinase were detected with the image analyzer 680 (GE Healthcare).
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bioRxiv - Biophysics 2020Quote: ... and 5% glycerol at pH = 5.9 for HiTrap™ Heparin HP column (GE Healthcare Lifescience, 17040601). p53-R248Q is eluted with linear gradient of NaCl ...
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bioRxiv - Biophysics 2021Quote: ... 1□mM DTT and 10% glycerol) using a PD MiniTrap G-25 sephadex columns (GE Healthcare) and the purified proteins were flash frozen in liquid N2 and stored at −80□°C ...
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bioRxiv - Biochemistry 2019Quote: ... 5% (vol/vol) glycerol and 5 mM β-mercaptoethanol using a HiTrap Desalting column (GE Healthcare) and the GST tag was cleaved with GST-TEV protease overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 10% (w/v) glycerol) by size exclusion chromatography using a Superdex200 16/600 column (GE Healthcare). Aliquots were flash frozen in liquid nitrogen and stored at −80°C until further use ...
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bioRxiv - Molecular Biology 2022Quote: ... 10% (v/v) glycerol) before loading on to a third affinity column (HiTrap Heparin HP, GE Healthcare). A step gradient (25-50-75-100% ...
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bioRxiv - Microbiology 2019Quote: ... 10% glycerol) and was subjected to size-exclusion chromatography (Superdex 200 10/300 GL, GE Life Sciences) to further purify the protein and was snap frozen in liquid nitrogen and stored in FtsZ storage buffer at −80 °C.
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bioRxiv - Immunology 2023Quote: ... 10% glycerol) and further purified by size-exclusion chromatography on a Superose 200 Increase column (GE Healthcare).
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bioRxiv - Biochemistry 2024Quote: ... size exclusion chromatography was followed by glycerol removal using a PD MiniTrap G-25 column (GE Healthcare) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... Glutathione was removed and CMG2 exchanged into HBS-T with 50% glycerol using Sephadex G50 (GE Life Sciences) on an Äkta Start chromatography system.
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bioRxiv - Molecular Biology 2021Quote: ... 10% (v/v) glycerol) by gel filtration using a Superdex 200 Increase 10/300 GL column (GE Healthcare). Fractions containing GapR were identified by SDS-PAGE/Coomassie staining ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM MgCl2 + 10% glycerol) was injected manually into a Superdex 200 Increase 10/300 GL (GE Healthcare) matrix column at a constant flowrate of 0.4ml/min ...
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bioRxiv - Biochemistry 2021Quote: ... [γ-32P] (Hartmann Analytic) using T4 polynucleotide kinase (Thermo Fischer) and subsequently purified using Illustra Microspin G25 columns (GE healthcare). Recombinant EB1 (0.5 ...
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bioRxiv - Cell Biology 2021Quote: ... The kinase complex was isolated using IgG sepharose 6 Fast Flow and Calmodulin sepharose 4B affinity resin (both GE Healthcare). In the last step of all bacterially expressed proteins ...
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bioRxiv - Biochemistry 2022Quote: ... 2% glycerol and 250 mM imidazole and further purified on a Superdex 200 HiLoad 16/600 column (GE Healthcare) in the gel filtration buffer (30 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... 10% glycerol (including 100 mM NaCl and 0.5 mM DTT for PSG)) Nickel Sepharose Fast Flow column (GE Healthcare). Proteins were eluted with 250 mM imidazole and dialysed overnight into 50 mM Tris pH 7.8 (PDZ3 ...
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bioRxiv - Biophysics 2020Quote: ... 20 % glycerol (buffer A) plus 20 mM imidazole and incubated with Ni2+-Sepharose resin (GE Healthcare Buckinghamshire, United Kingdom) (5.5 ml bed volume of Ni2+-Sepharose was used per g of wet weight cells ...
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bioRxiv - Microbiology 2022Quote: ... end-labelled with [γ-32P ATP] (1000 Ci nmol-1) using T4 polynucleotide kinase and purified from free label by Sephadex G-50 spin columns (GE Healthcare). Increasing amounts of purified regulators were incubated with appropriately end-labelled DNA probes in a total volume of 10 µl binding mix (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... and the phosphorylated kinase domain of FGFR1 was collected after size exclusion chromatography purification using Superdex 75 10/300 GL column (GE Healthcare). To prepare the dephosphorylated form ...
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bioRxiv - Bioengineering 2020Quote: ... 10% glycerol (v/v) at 4°C and re-loaded onto a Ni-NTA column (HisTrap™ HP, GE Healthcare). An imidazole gradient was applied (from 20mM to 500mM ...
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bioRxiv - Cell Biology 2020Quote: ... 5% glycerol (w/v, 0.1% Triton X-100) and mixed with 50 µl Glutathione Sepharose 4 Fast Flow (GE Healthcare) slurry ...
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bioRxiv - Microbiology 2019Quote: ... Purified protein was immediately exchanged into TEV cleavage buffer (50 mM Tris-HCl pH 8.0, 10% glycerol) using a 50 ml desalting column (GE Healthcare). For His-tag cleavage ...
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bioRxiv - Biochemistry 2020Quote: ... 10% glycerol and 0.1% triton X100 by using a desalting column PD Spin Trap G25 (GE healthcare: 28-9180-04). This FSS-PscN solution was then labeled with the Monolith Protein Labeling Kit RED-NHS (MO-L001 ...
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bioRxiv - Biochemistry 2021Quote: ... Purified Ycf1p NBD1 and NBD1-R were exchanged into the fluorescence buffer (20 mM Na3PO4, 2% glycerol, 150 mM NaCl, pH 7.3) by size exclusion chromatography (Superdex75, GE Healthcare). MgATP-bound and apo NBD1 and NBD1-R were generated by adding 2 mM MgATP or 2 mM EDTA ...
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bioRxiv - Bioengineering 2022Quote: ... the supernatant was filtered using 0.2 μm filters and buffer exchanged in storage buffer (20 mM Tris-HCl pH7.4, 200 mM NaCl and 5% glycerol) using PD-10 desalting columns (GE healthcare). The desalted sample then was aliquoted and stored at -80°C until the one-step purification/ immobilisation.
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bioRxiv - Microbiology 2020Quote: ... Bacteria were pelleted and lysed by sonication in lysis buffer (Tris 100mM, NaCl 0.5M, glycerol 5%) and eGFP was purified using fast flow His-affinity columns (GE Healthcare) and eluted with 500 mM imidazole ...