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Citations for GE Life Sciences :
601 - 650 of 1028 citations for Cow Flotillin 2 FLOT2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... of each peptide was serially diluted 2-fold in a 1:10 dilution of phosphate-buffered saline (PBS) (Hyclone; GE Healthcare, Pittsburgh, PA) in dH2O (10%PBS) ...
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bioRxiv - Microbiology 2021Quote: ... The MxiN and Spa33 were combined with an excess of MxiN and the mixture was concentrated to a final volume of 2 mL for injection onto a Hi Load 26/600 Superdex 200 pg column (GE Healthcare Life Sciences). The protein absorbance at 280 nm was monitored to detect protein elution on an AKTA system (GE Healthcare Life Sciences) ...
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bioRxiv - Genomics 2021Quote: ... DNA was stained with 0.2 µg/ml ethidium bromide (EtBr) (Nacalai Tesque, 14631-94) and detected using a Typhoon FLA9000 gel imaging scanner (GE Healthcare, Chicago, Illinois). Gel images were processed using ImageJ 1.8.0 (NIH ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1 mM ATP) overnight at 4°C and loaded onto a 2-ml Glutathione-Sepharose 4B (GE Healthcare Life Sciences, Piscataway, NJ, USA) column pre-charged with GST-N-WASp-VCA (Nolen & Pollard ...
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bioRxiv - Cell Biology 2021Quote: ... of SARS-CoV-2: the sequence encoding the N-terminally GST-tagged cytoplasmic domain of S were cloned into the vector pGEX6p2 (GE Healthcare Life Sciences). For GST-S (1255-1273 ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mg of each of the remaining lysates was diluted four-fold with IP buffer plus 0.1% v/v Triton X-100 and incubated for 2 hr at 4 °C with protein G-Sepharose (GE Healthcare catalog no. 17061801), pre-bound to HA-specific antibody (rabbit anti-HA from Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... Blots were UV cross-linked and baked at 80°C for 2 h prior to pre-hybridization at 42°C for 1 h in Rabid-Hyb Buffer (GE Healthcare Life Sciences). Hybridization with α-32P-ATP end-labeled DNA oligo probes was performed at 42°C for 2-16 h ...
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bioRxiv - Biochemistry 2020Quote: ... lysate was recovered and incubated for 2 h with pre-equilibrated Ni-NTA beads (Ni Sepharose 6 Fast Flow, GE Healthcare Life Sciences) in a binding buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2021Quote: ... of L-enantiomeric peptides CDP1 and CDP7 binding to SARS-CoV-2 3CLpro was determined by Surface Plasmon Resonance Spectroscopy (SPR) using a Biacore 8K instrument (GE Healthcare, Uppsala, Sweden). The peptides were immobilised on two separate channels on a series S CM-5 sensor chip (Cytiva ...
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bioRxiv - Cell Biology 2021Quote: ... hMBs and C2C12 cells were differentiated in a differentiation induction medium (DIM) consisting of DMEM with 2% horse serum (HyClone; GE Healthcare, UT, USA) and PS (Nihashi et al. ...
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bioRxiv - Neuroscience 2022Quote: ... installed prone on the MRI bed (Fig 1) and anaesthesia was immediately switched to 1.5-2% isoflurane (28) in medical air through a respirator (Aestiva, GE Healthcare, Datex-Ohmeda, USA). The respirator allowed continuous control of respiration rates ...
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bioRxiv - Biochemistry 2022Quote: ... pre-equilibrated with buffer B (buffer A supplemented with 1 mM DTT and 2 mM EDTA) connected to an AKTA FPLC system (GE Healthcare Life Sciences). Fractions corresponding to the elution peaks were analyzed by electrophoresis to assess the presence of MreB ...
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bioRxiv - Neuroscience 2022Quote: ... and soluble form of VAMP-2 lacking the transmembrane domain (VAMP-21-96) were purified using a Mono S column (GE Healthcare, Piscataway, NJ) as described previously26 ...
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bioRxiv - Immunology 2023Quote: ... Correctly conformed HLA.A2-peptide complexes were purified using Ni2+-NTA agarose chromatography (HisTrap excel, 2 × 5 ml2 columns connected in series; GE Healthcare Life Sciences) followed by size-exclusion chromatography (Superdex 200 10/300 GL ...
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bioRxiv - Neuroscience 2023Quote: ... A 20-μl droplet of virus inoculum containing 8X106 plaque-forming unit (PFU) of PRV-Becker, resuspended in medium (Dulbecco modified Eagle medium, 2% fetal calf serum and antibiotics) (Hyclone, GE Healthcare life sciences), was applied onto the abraded area of the skin ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated to 2-3 mL for separation on exclusion size chromatography (HiLoad 16/600 SuperdexTM 75pg, GE Healthcare; ÄKTA Avant). Finally ...
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bioRxiv - Biophysics 2023Quote: ... size exclu-sion chromatography was conducted by injecting 2-5 mL sample onto HiLoad 16/600 Superdex 200 Column (GE Healthcare Life Sciences) connected to a BioRad NGC Quest 10 FPLC system ...
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bioRxiv - Biochemistry 2023Quote: The Exosc3 open reading frame encoding the mouse EXOSC3 protein was cloned into pGEX-6P-2 plasmid (GE Healthcare Life Sciences (now Cytiva)) to create an N-terminally glutathione-S-transferase (GST ...
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bioRxiv - Biochemistry 2023Quote: ... was then incubated for 1 hour at 4°C on a rotating platform with 2 mL Sepharose 2B resin (GE Healthcare Life Sciences) suspended in 2 mL kinase buffer (50 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... was used according to manufacturer’s guideline to clone cDNAs into pUASTattB (kindly provided by K. Basler, University of Zurich) and pGEX-6P-2 (GE Healthcare Life Sciences). Full length (FL ...
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bioRxiv - Microbiology 2024Quote: ... MUC5B was purified using a Bio-Rad NGC fast protein liquid chromatography (FPLC) system equipped with an XK 50 column packed with 2 L of Sepharose CL-2B resin (GE Healthcare Bio-Sciences). Mucin-containing fractions were identified using a periodic acid-Schiff’s reagent assay and analysis of UV absorbance at 280 nm from FPLC ...
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bioRxiv - Cell Biology 2020Quote: ... Reverse transcription was performed using the First-Strand cDNA Synthesis Kit (GE Healthcare). Quantitative RT–PCR was performed using qPCR MasterMix Plus Low ROX (Eurogentec ...
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bioRxiv - Cell Biology 2020Quote: ... The bands were visualized after incubation with ECL chemiluminescence kit (Amersham, GE Healthcare). An anti-tubulin antibody was used as a loading control (monoclonal mouse anti-a-Tubuline ...
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bioRxiv - Cell Biology 2020Quote: ... Revelation was performed using an enhanced chemiluminescence detection system (ECL kit, GE Healthcare). Primary antibodies used were ...
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bioRxiv - Neuroscience 2021Quote: ... The proteins were detected using enhanced chemiluminescence (ECL) detection kit (GE HealthCare, RPN2235).
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bioRxiv - Molecular Biology 2021Quote: ... purified with mini columns (GFX PCR DNA & gel band purification kit, GE Healthcare) is described in this protocol (dx.doi.org/10.17504/protocols.io.brzsm76e ...
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bioRxiv - Microbiology 2019Quote: Protein concentration was measured with the “2D Quant kit” (GE Healthcare Life Sciences), according to the supplier’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Revelation was carried out using the ECL Plus western blotting kit (GE Healthcare). Primary antibody used for western blotting (WB ...
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bioRxiv - Neuroscience 2021Quote: ... HRP-conjugated secondary antibodies signal was revealed using ECL Prime kit (GE Healthcare) and captured using the ChemiDoc imager (BioRad ...
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bioRxiv - Cancer Biology 2021Quote: ... Antigen revelation was performed using the ECL kit (GE Healthcare, Chicago, IL, USA) and images were acquired on a MyECL machine (Thermo Fisher Scientific ...
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The anti-lipidemic drug simvastatin modulates Epigenetic Biomarkers in the Amphipod Gammarus locustabioRxiv - Pharmacology and Toxicology 2020Quote: ... together with the illustraTM RNAspin Mini RNA Isolation Kit (GE Healthcare, United Kingdom), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The Amersham ECL Western Blotting Detection Reagent Kit (GE Healthcare Life Sciences, UK) was used for final detection of proteins on the membrane.
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bioRxiv - Immunology 2022Quote: ... A low molecular weight gel filtration calibration kit (product code-28403841) (GE Healthcare) was used for calibrating the column ...
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bioRxiv - Pathology 2021Quote: ... and then visualized using a Western Blotting Detection Kit (GE Healthcare, cat#: RPN2106). Antibodies used in this study are listed in Supplementary Table 2.
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bioRxiv - Molecular Biology 2020Quote: ... Immunoblots were analysed using Amersham™ ECL™ Prime Kit from GE Healthcare Life Sciences (Waukesha ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA was extracted using the illustra RNAspin Mini Isolation kit (GE Healthcare). Reverse transcription was performed using oligo-dT and random hexamer primers with an in-house enzyme produced by the Protein Technologies Unit at CRG ...
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bioRxiv - Microbiology 2022Quote: ... and amplicons were purified using the Gel Band Purification kit (GE Life Sciences), re-surveyed on Nanodrop® and delivered to DNA Sanger sequencing at “laboratório de sequenciamento de ácidos nucleicos da FCFRP-USP” (Brazil) ...
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bioRxiv - Microbiology 2022Quote: ... pneumophila gDNA was isolated using the Illustra genomicPREP DNA spin kit (GE Healthcare) and used as a template for cloning sidI and mesI into the indicated plasmid vectors ...
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bioRxiv - Biochemistry 2022Quote: Samples were precipitated with the SDS-PAGE Clean-Up Kit (10074304 GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2020Quote: ... signals were revealed using the ECL Prime western blotting Detection Kit (GE Healthcare), and visualized with a ChemiDoc XRS Touch Imaging System (Bio-Rad) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Horseradish peroxidase conjugated secondary antibodies and enhanced chemiluminescence reagents (ECL kit) (GE Healthcare) were used for detection ...
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bioRxiv - Molecular Biology 2020Quote: ... purified with mini columns (GFX PCR DNA & gel band purification kit, GE Healthcare), and sequenced in ABI3500XL Series automatic sequencer (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2021Quote: ... The column was calibrated with a high molecular weight calibration kit (GE Healthcare) in the same buffer without detergent and lipid.
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bioRxiv - Genetics 2021Quote: ... and HAP1 cell pellets using the illustra triplePrep Kit (GE Healthcare Life Sciences) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... 30mg of placenta tissue was disrupted using a sample grinding kit (GE healthcare) and RLT buffer containing β-mercaptoethanol ...
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bioRxiv - Neuroscience 2022Quote: RNA extraction was performed using illustra RNAspin Mini Kit (GE Healthcare Life Sciences) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... The column was first calibrated with standard molecular weight calibration kit (GE Healthcare) made of blue dextran containing Ferritin (440 kDa) ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting western-blots were developed using an ECL Prime Kit (GE Healthcare) and detected with an ImageQuant 800 (Amersham).
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bioRxiv - Evolutionary Biology 2022Quote: ... FACS population were amplified using Ready-To-Go Genomiphi V3 kit (GE Healthcare), followed by purification and Illumina HiSeq sequencing (paired-end 2 × 151 bp ...
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bioRxiv - Genetics 2023Quote: ... A radiolabeled PCR probe was prepared by random priming (GE Healthcare, Megaprime kit) using α-P32 dCTP and incubated with the membrane in commercial buffer (Ambion ...