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Citations for GE Life Sciences :
1 - 50 of 5743 citations for Copper;5 10 15 20 tetrakis 4 methylphenyl porphyrin 22 24 diide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... After sonication the lysate was clarified by centrifugation (22 500 rpm/61 236 x g, 20 min, 4 °C) and applied to a HisTrap FF column (GE Healthcare) equilibrated with 10 column volumes (CV ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were filtered and then pelleted at 300 g for 10 minutes at 4°C before being resuspended in 22% Percoll (GE Healthcare) and centrifuged at 900 g for 20 minutes with acceleration set to 4 and deceleration set to 1 in order to remove cellular debris ...
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bioRxiv - Immunology 2024Quote: ... Samples were centrifuged at 220xg for 10 min at 4°C and then resuspended in a 22% Percoll solution (GE Healthcare) with a PBS layer floated on top ...
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bioRxiv - Immunology 2024Quote: ... Cells were filtered through 70 µm cell strainers and then centrifuged at 300xg for 10 minutes at 4°C before being resuspended in 22% Percoll (GE Healthcare) and centrifuged at 950xg for 20 minutes (acceleration=4 ...
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bioRxiv - Microbiology 2021Quote: ... (5) Source 15 RPC resin (GE Healthcare) chromatography using a linear gradient from 0 % to 86 % (v/v ...
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bioRxiv - Physiology 2020Quote: ... Blots were then washed in TBST and incubated for 1 h at 22-24°C with secondary HRP-conjugated antibodies (GE Healthcare) diluted 5000-fold in 5% skim milk in TBST ...
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bioRxiv - Immunology 2022Quote: ... and resuspended in 24 mL of RT 70% PercollTM (1.088 g/mL at 22 °C; GE Healthcare Life Sciences 17-0891-01). Aliquots of 8 mL cell/PercollTM suspension were overlayed with 4 mL HBSS and centrifuged at 400 xg for 30 minutes RT ...
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bioRxiv - Microbiology 2022Quote: ... The purified protein was then dialyzed against 10 mM Tris HCl pH 8.0 and loaded on MonoQ 5/15 column (GE Healthcare) to be eluted using a step gradient of 240 mM and 400 mM NaCl in the same buffer.
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bioRxiv - Biochemistry 2022Quote: ... centrifuged at 13,300 rpm for 15 mins at 4°C and then loaded onto a Superose 6 Increase 10/300 column (GE Healthcare) pre-equilibrated with CENP-E gel filtration buffer (50 mM HEPES pH 7 ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was cleared by two rounds of 20 min centrifugation at 45,000 x g at 4°C and loaded onto a 5-mL HisTrap column (GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Molecular Biology 2023Quote: ... centrifuged at 16,000 g for 20 min at 4°C and then injected into Superose 6 5/150 increase column (GE Healthcare) pre-equilibrated with analytical size-exclusion chromatography buffer (50 mM HEPES ...
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bioRxiv - Cell Biology 2022Quote: ... Images were acquired from 15 fields at 5- or 10-minute intervals using a DeltaVision Elite Deconvolution Microscope (GE Healthcare Biosciences) with a 60x oil immersion objective and a Front Illuminated sCMOS camera ...
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bioRxiv - Biochemistry 2020Quote: ... SDS-PAGE gels (15-well NuPAGE 4–12% BisTris, GE Healthcare) revealed a single band for the purified enzymes ...
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bioRxiv - Immunology 2020Quote: ... Dilution series of TBK1 or IRF-3 (1.25, 2.5, 5, 10, 20 μM) in 1× HBS-EP+ buffer (GE Healthcare) were injected over the sensor chip at a flow rate of 30 μL/min ...
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bioRxiv - Biochemistry 2020Quote: ... 10.2 µM protein was incubated with 100 µM probe and 0.5 mM TCEP for ∼20 h at 4 °C and free probe was removed by desalting using a 5 mL HiTrap desalting column (GE Healthcare).
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bioRxiv - Biophysics 2021Quote: ... while a Tricorn 5/20 column (GE Healthcare Life Sciences ...
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bioRxiv - Molecular Biology 2020Quote: ... the clarified milk was thawed and loaded at 150 cm/h and 90 cm/h onto a 22 mL (1.6 x 11 cm) and 40 mL (1.6 x 20 cm) rPA Sepharose FF (GE Healthcare, Uppsala, Sweden) Protein A affinity chromatography column equilibrated with PBS for samples with a 25 mL and 100 mL milk starting volume ...
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bioRxiv - Immunology 2024Quote: ... Filtered suspensions were centrifuged at 220g for 10 minutes and resuspended in 5mL of 22% Percoll (GE Healthcare) in Myelin Gradient Buffer (5.6mM NaH2PO4•H2O ...
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bioRxiv - Biophysics 2022Quote: ... the supernatant was dialyzed against 20 mM Tris-HCl pH 8.0, centrifuged (15 min, 48000xg, 4 °C) and loaded onto a 15 ml Q-sepharose FF (GE Healthcare) column equilibrated with the same buffer ...
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bioRxiv - Neuroscience 2020Quote: ... and centrifuged using 22% percoll (GE Healthcare) to remove myelin debris ...
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bioRxiv - Bioengineering 2022Quote: ... the supernatant was filtered using 0.2 μm filters and buffer exchanged in storage buffer (20 mM Tris-HCl pH7.4, 200 mM NaCl and 5% glycerol) using PD-10 desalting columns (GE healthcare). The desalted sample then was aliquoted and stored at -80°C until the one-step purification/ immobilisation.
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bioRxiv - Biophysics 2021Quote: ... A Superdex 200 HR-10/30 column (24 mL; GE Healthcare Life Sciences) was used with isocratic elution in 50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... in the 24-fraction setup using pI 3-10 IPG strips (GE Healthcare) for 50 kVh ...
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bioRxiv - Microbiology 2024Quote: ... and then was purified by Superdex200 10/300 GL (GE Healthcare, 24 mL) (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... Refolded and soluble proteins were concentrated using Vivaspin 20 centrifugal concentrators (10 kDa and 5 kDa molecular weight cutoff) and further purified on Superdex S75 columns (GE Healthcare) in SEC buffer (50 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... Pooled fractions from ion-exchange chromatography were filtered through a 5 µm syringe filter and loaded onto a 22 mL Source 15ISO hydrophobic interaction column (GE Healthcare) equilibrated in buffer C (50 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... Sec23/24 was affinity purified following application to a 5 mL HisTrap column (GE Healthcare) equilibrated in lysis/binding buffer (20 mM HEPES (pH 8.0) ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... The complex was incubated for 10 min at 22°C before size exclusion chromatography using a Superdex 200 Increase 3.2/300 column (GE Healthcare) at 22°C ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μl of purified sample (3.8 mg/mL TbMORN1(7-15) and 2.6 mg/ml TgMORN1(7-15)) were injected onto a Superdex 200 10/300 (GE Healthcare) column and the flow rate was set to 0.5 ml/min ...
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bioRxiv - Neuroscience 2020Quote: ... at 10-20 μg/mL concentration in 10 mM acetate solution (GE Healthcare) at pH 4.5 to reach a final surface ligand density of around 2000-4000 response units (RUs) ...
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bioRxiv - Neuroscience 2023Quote: ... at 10–20 μg/mL concentration in 10 mM acetate solution (GE Healthcare) at pH 4.5 to reach a final surface ligand density of around 2000–4000 response units (RUs) ...
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bioRxiv - Neuroscience 2021Quote: ... The pellet was resuspended in 22% Percoll (GE Healthcare) and centrifuged at 900 g ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was centrifuged at 9,400× g at 4 °C for 20 min and the supernatant was applied onto a 5 mL His-Trap TALON crude column (GE Healthcare Bioscience, Piscataway, NJ, USA) pre-equilibrated with deionized water and Talon binding buffer ...
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bioRxiv - Biophysics 2022Quote: ... then analyzed in 15% urea-PAGE and visualized using Amersham Typhoon 5 (GE Healthcare). Product bands were quantified using ImageJ and cleaved fraction was calculated using the intensity of product bands divided by input intensity.(Schneider et al. ...
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bioRxiv - Microbiology 2020Quote: ... 4-20% (p/v) Amersham ECL Gel (GE Healthcare, Chicago, Illinois, EEUU), using the ECL Gel Box system (GE Healthcare ...
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bioRxiv - Molecular Biology 2024Quote: ... The resin was then incubated overnight at 4 °C with 24 units of PreScission protease (GE Healthcare, US) to cleave the ScParB variant from the GST tag ...
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... containing 10% DMSO (Carl Roth) and 20% FBS (GE Healthcare). For long-term storage ...
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bioRxiv - Biochemistry 2020Quote: ... concentrated eluate was fractionated on a 24 ml Superdex 200 Increase 10/300 GL (GE Healthcare) gel filtration column in buffer A / 300 mM NaCl / 1 mM DTT ...
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bioRxiv - Biochemistry 2020Quote: ... The concentrate was gel-filtered on a 24 ml Superdex 200 10/300 GL (GE Healthcare) column equilibrated in 25 mM HEPES-KOH pH 7.5 / 1 mM EDTA / 10 % glycerol / 300 mM KOAc / 1 mM DTT ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleavage products were separated by gel electrophoresis on 15% denaturing polyacrylamide gel containing 7.5M urea and 24% formamide and visualized using a Typhoon 9400 Scanner (GE Healthcare Chicago, IL, USA). Activity assays performed with purified Argonaute proteins were carried out with 17 nt long guide G-2 (DNA ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the sections were collected on coverslips (22 mm x 22 mm) previously pretreated with a silanization solution (0.3% (v/v) bind-silane (GE Healthcare Life Sciences, 17-1330-01), 0.1% (v/v ...
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bioRxiv - Biochemistry 2024Quote: ... RRM2 (>5 mg mL-1) was injected onto a 24 mL Superdex 75 size exclusion column (GE Healthcare) at a flow rate of 1 mL min-1 ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% (HeLa) or 15% (Tig-1) fetal bovine serum (HyClone, GE Healthcare Life Sciences ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 µL of Protein A Sepharose 4 FF (GE Healthcare Japan, Tokyo, Japan) was added and mixed with rotation at 4°C for 3 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein samples (20 µg) were separated on 15% SDS-PAGE and transferred to Protran nitrocellulose membranes (GE Healthcare). Membranes were blocked overnight at 4°C with 5% milk in TBS-T (20 mM Tris pH 7.6 ...
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bioRxiv - Immunology 2022Quote: ... Whole blood (20 mL) was layered upon a 15 mL Ficoll-Paque cushion (GE Healthcare, 17-1440-02) in 50 mL conical tubes ...
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bioRxiv - Biochemistry 2021Quote: ... and loaded onto a 24 mL Superdex 200 10/300 GL (GE Healthcare, Cat# 17-5174-01) size exclusion column equilibrated in 20 mM Tris [pH 8.0] ...
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bioRxiv - Microbiology 2020Quote: ... and size-exclusion chromatography was performed using a 24-ml Superdex 200 10/300 column (GE Healthcare) with 20 mM Tris (pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... and loaded onto a 24 mL Superdex 200 10/300 GL (GE Healthcare, Cat# 17-5174-01) size exclusion column equilibrated in 20 mM Tris [pH 8.0] ...
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bioRxiv - Cell Biology 2023Quote: ... and loaded onto a 24 mL Superdex 200 10/300 GL (GE Healthcare, Cat# 17-5174-01) size exclusion column equilibrated in 20 mM Tris [pH 8.0] ...