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Citations for GE Life Sciences :
51 - 100 of 1140 citations for Complement Factor B CFB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The RBD/RA of B-RAF (residues 150 -233) and RASSF5 (residues 199 - 367) were subcloned into pGEX-4T2 (GE Healthcare) to produce N-terminal GST fusion proteins ...
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bioRxiv - Plant Biology 2022Quote: ... coli strain BL21 with protein induction when OD600 reached 0.5 with 1 mM IPTG (isopropyl b-D-1-Thiogalactopyranoside) for 4 h at 37°C and then purified over a glutathione Sepharose 4B column (GE Healthcare). The eluted samples were then resolved by SDS-PAGE and stained by Coomassie brilliant blue R-250 to evaluate purity ...
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bioRxiv - Biophysics 2023Quote: ... we repeated the dilution in Buffer B to 18 mS/cm conductivity and loaded it onto HiTrap SP columns (GE Healthcare). For elution ...
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bioRxiv - Microbiology 2023Quote: ... Peak fractions were collected and diluted with buffer B to a conductivity of 18 mS/cm and loaded onto HiTrap SP columns (GE healthcare). A linear gradient of buffer B containing 1 M NaCl was used for elution ...
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bioRxiv - Microbiology 2023Quote: ... The sample was additionally diluted with buffer B to a conductivity of 18 mS/cm and loaded onto a Heparin column (GE healthcare). The protein was eluted with a linear gradient of buffer B containing 1 M NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... 5mM BME) with elution buffer B (buffer A with 500mM Imidazole) on a 5mL His-Trap FF Crude Nickel Column (GE Healthcare). Fractions containing Pol θ were pooled and were separated further on a 5mL HiTrap Heparin HP (GE Healthcare ...
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bioRxiv - Systems Biology 2024Quote: ... SP3 magnetic beads were prepared as follows: per 10 samples 20 µL of each Sera-Mag Speed Beads A and B (GE Healthcare) were mixed 1:1 ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies were detected with peroxidase-conjugated secondary antibodies (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... diluted two-fold against fresh buffer B and then manually applied at room temperature to a 5 mL HiTrap Heparin cation exchange column (GE Healthcare # 17040701) that had been pre-equilibrated in buffer B ...
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bioRxiv - Microbiology 2020Quote: ... 300 ml NaCl and eluted via a gradient of increasing imidazole (0-500 mM, Buffer B) using a ÄKTAPurifier™ System (GE Healthcare). After this first purification ...
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bioRxiv - Plant Biology 2022Quote: ... induced for 3 h induction by IPTG (isopropyl b-D-thiogalactopyranoside) and soluble extracts were purified using GST-sepharose 4B (GE Healthcare, Sweden) as described elsewhere (Geisler et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were detected using horseradish peroxidase-conjugated secondary antibodies (GE Healthcare). To detect the deGRadFP expression from the hb-deGRadFP construct ...
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bioRxiv - Biochemistry 2019Quote: ... via an Fc antibody using the Human Antibody Capture Kit (GE Healthcare). Immobilization levels ranged from 7,800-9,000 response units (RU ...
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bioRxiv - Microbiology 2020Quote: ... antibody with HRP-conjugated anti-mouse secondary antibody (GE Healthcare or Promega). In addition to the experimental samples ...
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bioRxiv - Pathology 2021Quote: ... horseradish peroxidase linked antibodies were used as a secondary antibody (GE Healthcare). Each signal was detected using Immunostar Zeta or LD (Fuji film ...
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bioRxiv - Biochemistry 2021Quote: ... Secondary antibodies (GE Healthcare) were used at 1:5,000 for 1 h at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... the supernatant was loaded onto a HisTrap HP 1-mL affinity column and eluted using a gradient of 10 to 500 mM imidazole in buffer B using an ÄKTA start FPLC module (GE Healthcare, Uppsala, Sweden). Fractions were analyzed by SDS–polyacrylamide gel electrophoresis ...
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bioRxiv - Bioengineering 2022Quote: We deployed vendor-neutral pulse sequences developed in RTHawk v3.0.0 (rc4-28-ge3540dda19) (HeartVista Inc., CA, USA) on three 3T systems: (G1) GE Discovery 750 software version DV25 (R02_1549.b) (GE Healthcare, Milwaukee, MI, USA), (S1 ...
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bioRxiv - Biochemistry 2020Quote: ... and fractions from 15 % to 35 % of buffer B were collected and applied to 5ml HisTrap HP column (GE Healthcare Life Sciences, USA) equilibrated with 5 volumes of buffer C (20 mM Tris (pH 7.6) ...
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bioRxiv - Biochemistry 2022Quote: ... pre-equilibrated with buffer B (buffer A supplemented with 1 mM DTT and 2 mM EDTA) connected to an AKTA FPLC system (GE Healthcare Life Sciences). Fractions corresponding to the elution peaks were analyzed by electrophoresis to assess the presence of MreB ...
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bioRxiv - Cell Biology 2019Quote: ... the primary antibody was visualized using 2~5 μg/ml secondary antibody towards specific species of primary antibody labeled with Cy3B (GE Healthcare) or Atto 488 (Sigma-Aldrich ...
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bioRxiv - Physiology 2021Quote: ... Primary antibodies were detected with HRP-conjugated secondary antibodies (GE Healthcare, NA934, NA931), diluted 1:10,000 ...
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bioRxiv - Biochemistry 2019Quote: The interaction of the recombinant heterodimeric (‘monomer’) and homodimeric (‘dimer’) human G6b-B extracellular domain with different ligands was quantified using a BIAcoreTM 8K instrument (GE Healthcare, Little Chalfont, UK). Recombinant G6b-B proteins were immobilized on CM5 sensor chips (GE Healthcare ...
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bioRxiv - Plant Biology 2023Quote: ... Active fractions were combined and exchanged into buffer B (50 mM Tris-HCl, pH 7.5) before being applied to a Mono Q™ HR 5/5 column (GE Healthcare, Piscataway, NJ, USA) equilibrated with the same buffer ...
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bioRxiv - Plant Biology 2021Quote: ... Monoclonal anti-actin (plant) antibody detected by the secondary antibody (GE Healthcare; NA931-1ML) was used as a loading control (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... The secondary antibodies were HRP-linked antibodies against rat or mouse IgG (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... antibodies and the secondary anti-rabbit horseradish peroxidase-linked IgG antibody (NA944V, GE Healthcare for western blotting and 7074S Cell signaling Technology for IHC ...
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bioRxiv - Biochemistry 2021Quote: ... HRP linked antibodies (GE Healthcare), respectively.
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bioRxiv - Biochemistry 2023Quote: ... Anti-GST antibody (GE Healthcare) was used for the detection of GST-tagged bromodomains.
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bioRxiv - Biochemistry 2021Quote: ... diluted to 1 mL with buffer B and added immediately onto an anion exchange resin (Q resin; GE Healthcare; 0.2 mL; equilibrated in buffer H) and incubated (5 min) ...
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bioRxiv - Genomics 2022Quote: ... 0.5ml of antibiotic antimycotic solution (10000 lg/ml streptomycin, 10000 U/ml penicillin G, and 25 lg/ml amphotericin B, HyCloneTM, GE Healthcare Life Sciences, Amersham, UK) and 6ml of foetal calf serum were used to make lymphocyte short-term medium ...
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bioRxiv - Cancer Biology 2020Quote: ... Secondary antibodies were horseradish peroxidase (HRP)-conjugated anti-mouse antibody (NA931, GE Healthcare Life Sciences) and HRP-conjugated anti-rabbit antibody (NA934V ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies were detected with appropriate secondary antibodies conjugated to horseradish peroxidase (GE Healthcare, Chicago, IL). Membranes were rinsed in PBS + 0.05% Tween-20 before visualization.
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bioRxiv - Microbiology 2019Quote: ... as primary antibody and an HRP-conjugated anti-mouse as the secondary antibody (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... Secondary antibodies include ECLTM anti-Rabbit IgG Horseradish Peroxidase linked whole antibody (#NA934V, GE Healthcare) and ECLTM anti-mouse IgG Horseradish Peroxidase linked whole antibody (#NA931V ...
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bioRxiv - Molecular Biology 2023Quote: ... and the secondary antibody (ECL anti-mouse IgG horseradish peroxidase linked whole antibody) (GE Healthcare) was applied for 1 h in 1% skimmed milk in TBST ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies were detected by an HRP-conjugated donkey anti-rabbit secondary antibody (GE Healthcare; # NA934V) incubated in PBST for 1h at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Peroxidase-labelled anti-mouse IgG antibody and anti-rabbit IgG antibody (GE Healthcare, Chicago, IL, USA) were used as secondary antibodies for immunoblotting ...
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bioRxiv - Microbiology 2020Quote: ... Antibodies were detected with appropriate secondary antibodies conjugated to horseradish peroxidase (GE Healthcare, Chicago, Il USA). Membranes were rinsed in PBS + 0.05% Tween-20 and visualized by ECL in an ImageQuant LAS 4000mini (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies were detected by an HRP-conjugated donkey anti-rabbit secondary antibody (#NA934V; GE Healthcare) or HRP-conjugated donkey anti-mouse secondary antibody (#NXA931 ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary horseradish peroxidase-conjugated antibodies (anti-mouse/rabbit IgG HRP-linked whole antibody donkey; GE Healthcare) were used ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-linked antibody (NA934; GE Healthcare) as secondary antibody.
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bioRxiv - Cell Biology 2019Quote: ... HRP-linked whole antibody (GE Healthcare) and polyclonal rabbit anti-goat (Dako) ...
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bioRxiv - Molecular Biology 2019Quote: ... HRP conjugated second antibodies (GE Healthcare) were used at a concentration of 1:5000 ...
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bioRxiv - Cancer Biology 2021Quote: ... Anti-mouse secondary antibody (GE Healthcare) was used at 1:10,000 dilution and anti-rabbit secondary antibody (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated secondary antibodies (GE Healthcare) were diluted 1:10,000 in TBS + 0.1% Tween-20 and applied to the membrane for 1 hr ...
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bioRxiv - Microbiology 2020Quote: ... IgG1 or IgG2a antibodies (GE Healthcare). The plates were washed and developed with 50 μl of TMB substrate per well until blue color appeared ...
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bioRxiv - Cell Biology 2019Quote: ... Species-appropriate secondary antibodies (GE Healthcare) and ECL Prime Western Blotting Detection reagent (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... Secondary antibody (GE Healthcare, Buckinghamshire, UK) was used in a 1:2000 titer in 5% milk/Tris-buffered saline–Tween 20 for 1 hour at room temperature with agitation ...
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bioRxiv - Synthetic Biology 2022Quote: ... or anti-rabbit antibody (GE Healthcare) as secondary antibodies ...