Labshake search
Citations for GE Life Sciences :
1 - 50 of 3159 citations for Ciprofloxacin Hcl 100 Ug Ml In Methanol 2 3 Carboxyl 13C3 99%; Quinoline 15N 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: ... PKAc was immobilized via standard N-hydroxysuccinimide (NHS) / 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) amine coupling on a CM5 (carboxyl methyl dextran) sensor chip (GE Healthcare). Before covalent immobilization of PKAc ...
-
bioRxiv - Physiology 2023Quote: ... Seramag speedbead carboxyl beads (GE Life Sciences) were prepared where a 1:1 mix of hydrophilic (Cat No ...
-
bioRxiv - Cancer Biology 2022Quote: ... a bead slurry was prepared by mixing 50 μl of Sera-Mag SpeedBeads Carboxyl Magnetic Beads (hydrophobic) with 50 μl of Sera-Mag SpeedBeads Carboxyl Magnetic Beads (hydrophilic) (both from GE Healthcare). The bead slurry was washed two times with 200 μl of MQ water using a magnetic rack ...
-
bioRxiv - Cell Biology 2021Quote: ... Hydrophilic and hydrophobic Cytiva Sera-Mag Carboxyl-Magnet-Beads (GE Healthcare) were mixed 1:1 and 2 μl beads (25 μg/μl ...
-
bioRxiv - Plant Biology 2021Quote: ... 100 mM DTT and 2% v/v IPG buffer pH 3– 10 (GE Healthcare) was applied to the strips ...
-
bioRxiv - Genomics 2022Quote: ... with 1.0x volume of 0.4% solution of SeraMag SpeedBeads® Carboxyl Magnetic Beads (GE Healthcare), followed by an incubation of 30-60 min ...
-
bioRxiv - Neuroscience 2022Quote: ... and an aliquot of synaptosomal suspension (2 ml) was placed onto a 3-ml Percoll discontinuous gradient (GE Healthcare) containing (in mM ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Purification column was prepared using 2-3 mL IMAC Sepharose 6 Fast Flow beads (GE Healthcare) and charged with 0.1 N Nickel sulphate solution ...
-
bioRxiv - Cell Biology 2020Quote: ... and 100 units/ml penicillin-100 μg/ml streptomycin (GE Healthcare, Logan UT). MCF7 cells used were authenticated to be 100% match to MCF7 cells (ATCC ...
-
bioRxiv - Cancer Biology 2021Quote: ... and 100 units/ml penicillin-100 μg/ml streptomycin (GE Healthcare, Logan UT) at 37 °C in 5% CO2 ...
-
bioRxiv - Microbiology 2022Quote: ... The remaining lysate supernatant was incubated for 2 h on a spinning wheel with 200 µl of high-Performance strepTactin Sepharose beads (#28-9355-99, GE healthcare). The beads were collected by centrifugation at 1600 g at 4°C for 5 min ...
-
bioRxiv - Cancer Biology 2020Quote: ... 100 U/ml penicillin and 100 mg/ml streptomycin (HyClone; GE Healthcare Life Sciences). All cells were routinely cultured in a humidity incubator at 37°C and 5% CO2 ...
-
bioRxiv - Microbiology 2020Quote: ... 20 mM Tris HCl pH 8.0) using Vivaspin filters with a 100 kDa molecular weight cutoff (GE Healthcare). Protein concentrations were determined using the Nanodrop method ...
-
bioRxiv - Microbiology 2022Quote: ... The supernatant was aspirated to the 5 mL mark and the samples were transferred to fresh 15 mL tubes containing 2 mL of 100% Percoll (GE Healthcare catalog no. 17-0891-01). Water was added to the 10 mL mark ...
-
Psi promotes Drosophila wing growth through transcriptional repression of key developmental networksbioRxiv - Developmental Biology 2020Quote: ... Sample clean-up and library preparation was carried out using Sera-Mag Speedbeads hydrophobic carboxyl magnetic beads (GE Healthcare, 65152105050250). Following bead cleanup ...
-
bioRxiv - Immunology 2022Quote: ... 3 ml Ficoll-Paque media (GE Healthcare) was pipetted into the bottom and the diluted 4 ml blood sample was carefully layered on top ...
-
bioRxiv - Microbiology 2022Quote: ... Ten µg of a 1:1 mixture of hydrophilic and hydrophobic carboxyl-coated paramagnetic beads (SeraMag, #24152105050250 and #44152105050250, GE Healthcare) were added for each µg of protein ...
-
bioRxiv - Molecular Biology 2023Quote: ... The magnetic bead mixes were prepared by combining equal parts of Sera-Mag Carboxyl hydrophilic and hydrophobic particles (09-981-121 and 09-981-123, GE Healthcare). The bead mix was washed three times with MS-grade water and resuspended in a working concentration of 10 µg/µl ...
-
bioRxiv - Cell Biology 2020Quote: ... was exchanged into labelling buffer (20 mM Tris-HCl pH 7.5, 100 mM NaCl) using size exclusion NAP10 columns (GE Healthcare). Protein-containing fractions were identified by protein electrophoresis and pooled ...
-
bioRxiv - Cell Biology 2019Quote: ... adjusted to 100 mM Tris-HCl pH 8.0 and 1 mM EDTA and loaded onto a StrepTrap HP column (GE Healthcare) pre-equilibrated in 100 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Plant Biology 2020Quote: ... Proteins were eluted from the column with a 10–200 mM imidazole gradient (100 mM Tris-HCl, pH 8.0, 200 mM NaCl) using an ÄKTA Prime Plus chromatography system (GE Healthcare). Fractions were collected and stored at 4°C.
-
bioRxiv - Microbiology 2023Quote: ... and 0.0036% H2O2 in 100 mM Tris-HCl (pH 9.35)) and imaged the chemiluminescence using an ImageQuant LAS-4000 mini Imaging System (GE Healthcare).
-
bioRxiv - Evolutionary Biology 2022Quote: ... Samples were homogenised by bead beating at 4°C in 500 μl Tris-HCl lysis buffer (100 mM, pH 6.8) containing 1% Protease Inhibitor Mix (GE Healthcare). A full and detailed description of the protocol for 2-dimensional gel electrophoresis (2-DE ...
-
bioRxiv - Immunology 2019Quote: ... the cells were resuspended in approximately 2 mL of 100% Percoll solution (Cat. no. 17-0891-01, GE Healthcare), and layered carefully on top of 3 mL of 80% and 40% Percoll solution ...
-
bioRxiv - Microbiology 2020Quote: ... Perkin Elmer with 100 Ci/ml for 2 hrs and immunoprecipitation of Fcγ fragments using CNBr-Sepharose (GE Healthcare) was performed as described previously (Sprague et al. ...
-
bioRxiv - Biophysics 2019Quote: ... The reaction was incubated at 37°C for 2-3 hours on an orbital shaker and loaded onto an anion-exchange colum (1 ml DEAE FF, GE Healthcare) equilibrated in 50 mM Tris-HCl pH 7.4 ...
-
bioRxiv - Biophysics 2022Quote: ... 250 μL protein at a concentration of 2-3 mg/mL was injected on a Superdex200 10/300 increase column (GE Healthcare) equilibrated with 20 mM Tris pH 7 ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... 100 U/mL penicillin/0.1 mg/mL streptomycin (GE Healthcare, USA), 1% L-glutamine ...
-
bioRxiv - Plant Biology 2020Quote: ... Proteins were eluted from the column with a 10–200 mM imidazole gradient (100 mM Tris-HCl, pH 8.0, 200 mM NaCl) using an ÄKTA Prime Plus chromatography system (GE Healthcare, Chicago, IL). Fractions were collected and stored at 4°C and the presence of GSTU7-His protein was confirmed by analyzing the collected fractions by SDS-PAGE.
-
bioRxiv - Cell Biology 2021Quote: ... The mass evaluated using UV as concentration source was 55.7 kDa for the 2 mg/ml sample.The Odinarchaeota samples were analysed by SEC-MALS (100 μl protein complex at 2 mg/ml) were passed over a Superdex 200 10/300 Increase GL column (GE Healthcare), in 20 mM Tris (pH 8.0) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Protein samples (100 μl at 2 mg/ml) were resolved using a Superdex 200 10/300 analytical gel filtration column (GE Healthcare) running at 0.5 ml/min at 25 °C in 10 mM K-HEPES pH 8.0 ...
-
bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated to 2-3 mL for separation on exclusion size chromatography (HiLoad 16/600 SuperdexTM 75pg, GE Healthcare; ÄKTA Avant). Finally ...
-
bioRxiv - Neuroscience 2021Quote: ... and transferred onto a methanol-activated PVDF membrane (GE Healthcare) at 60 V for 12 h at 4 °C ...
-
bioRxiv - Microbiology 2019Quote: ... Purified protein was immediately exchanged into TEV cleavage buffer (50 mM Tris-HCl pH 8.0, 10% glycerol) using a 50 ml desalting column (GE Healthcare). For His-tag cleavage ...
-
bioRxiv - Biochemistry 2021Quote: ... The periplasmic extract was adjusted to 50 mM Tris/HCl pH 9.0 (loading buffer) and loaded onto a 5 mL Q Sepharose anion exchange column (GE Healthcare). Proteins were eluted using a linear salt gradient (0-500 mM NaCl ...
-
bioRxiv - Biophysics 2022Quote: ... the supernatant was dialyzed against 20 mM Tris-HCl pH 8.0, centrifuged (15 min, 48000xg, 4 °C) and loaded onto a 15 ml Q-sepharose FF (GE Healthcare) column equilibrated with the same buffer ...
-
bioRxiv - Biochemistry 2019Quote: ... 25 mM Tris-HCl pH 7.5) prior to adding to a HiTrap QFF (1 mL) strong anion exchange column (GE Healthcare) equilibrated with 10 column volumes (CV ...
-
bioRxiv - Microbiology 2021Quote: ... The eluate was then diluted 3x with 20 mM Tris-HCl pH7.5 and passed over a 5 mL Q-sepharose column (GE Healthcare). The Flow through containing the MIF protein was then again loaded on a 5 mL CM sepharose column ...
-
bioRxiv - Biochemistry 2019Quote: ... The total eluate was diluted 1:2 with 25 mM Tris-HCl pH 8.0 and further purified by anion exchange chromatography using a 6 ml RESOURCE Q column (GE Healthcare) equilibrated in 95% AEX-A (25 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Immunology 2019Quote: ... 20U/ml Xuri IL-2 (GE Healthcare) was added ...
-
bioRxiv - Biochemistry 2024Quote: ... protein was loaded onto a pre-equilibrated (50 mM Tris-HCl 7.5 at 4 °C and 100 mM sodium chloride) Superdex 16/600 size exclusion column (GE Life Sciences, Chicago, IL) following Ni-NTA affinity chromatography to remove any protein aggregate ...
-
bioRxiv - Plant Biology 2023Quote: ... gel analysis were pooled and dialyzed against Tris-HCl buffer (30 mM Tris-HCl, pH 8) using PD-10 Desalting Columns (GE Healthcare). The protein concentration was determined by Bradford assays with BSA (bovine serum albumin ...
-
bioRxiv - Biochemistry 2021Quote: ... flow rate 0.8 ml/min) or Superdex 200 Increase 5/150 (~3 ml, flow rate 0.45 ml/min) columns (GE Healthcare) were used ...
-
bioRxiv - Developmental Biology 2022Quote: ... before being blotted onto methanol-activated 0.45μm PVDF membranes (GE Healthcare) at 300mA for 1 hour ...
-
bioRxiv - Immunology 2019Quote: ... 100 U/mL of Penicillin (GE Healthcare, catalog SV30010), and 100 µg/mL of Streptomycin (GE Healthcare ...
-
bioRxiv - Biophysics 2019Quote: ... and 100 U/mL HyClone penicillin-streptomycin (GE Healthcare) at 37°C and 5% CO2.
-
bioRxiv - Immunology 2019Quote: ... and 100 µg/mL of Streptomycin (GE Healthcare, catalog SV30010)) at 37 °C/5% CO2 ...
-
bioRxiv - Bioengineering 2021Quote: ... and 100 mg/mL streptomycin (HyClone, GE Healthcare Life Sciences), at 37 °C and 5% CO2 ...
-
bioRxiv - Biochemistry 2023Quote: ... and 0.5 ml of 100 kU DNase I (GE healthcare) for 10 minutes at 37°C ...
-
bioRxiv - Biochemistry 2020Quote: ... 2 ml of Chelating Sepharose Fast Flow (GE Healthcare) was added to a small plastic column and prepared for affinity purification by sequential washing with 1 column volume (CV ...