Labshake search
Citations for GE Life Sciences :
51 - 100 of 1162 citations for Chromosome 11 Open Reading Frame 53 C11ORF53 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2023Quote: ... 11 times through a NucleporeTM Track-Etch Membrane polycarbonate filter of pore size 400 nm (GE Healthcare Life Siences) and diluted to 4 mg/ml in reconstitution buffer ...
-
bioRxiv - Molecular Biology 2019Quote: ... fused to triple epitope tags were prepared by directionally cloning in-frame epitope tags into the BamHI and EcoRI restriction sites of pGEX-4T-1 (GE Healthcare Life Sciences #28954549). PCR amplicons were prepared from a PAGE-purified oligonucleotide template with forward primers containing a BglII restriction site and reverse primers containing HindIII–BamHI–EcoRI sites (Supplementary Table S1) ...
-
bioRxiv - Immunology 2021Quote: ... Biotin excess was removed by SEC on either a Superdex 200 10/300 column (GE Healthcare) for the RBD probe or a HiLoad Superose 6 16/600 column (GE Healthcare ...
-
bioRxiv - Biochemistry 2022Quote: ... and unincorporated biotin-17-dCTP was removed by using S-200 HR Microspin columns (GE Healthcare). The DNA was ethanol precipitated and resuspended in TE buffer ...
-
bioRxiv - Cell Biology 2022Quote: ... Biotin-labelled PCR amplicons were captured by mixing PCR products with streptavidin-sepharose beads (GE Healthcare). Vacuum Prep Tool with filter probes was used to capture sepharose beads with biotin labelled PCR amplicons ...
-
bioRxiv - Biochemistry 2021Quote: ... Biotin incorporation was assessed by incubating blots with streptavidin-HRP (1:1000 v/v, GE Healthcare), and vimentin levels estimated by western blot with anti-vimentin V9 monoclonal antibody (1:1000 v/v ...
-
bioRxiv - Microbiology 2023Quote: ... Biotin-labelled HCV 3’UTR RNA was immobilized on streptavidin-coated sensor chips (GE Healthcare Lifescience) to a final concentration of 300 Resonance Units (RU)/flow cell ...
-
bioRxiv - Cell Biology 2023Quote: ... Biotin incorporation was assessed by incubating blots with HRP-streptavidin (1:1000 v/v, GE Healthcare) and vimentin levels were estimated by western blot as described below ...
-
bioRxiv - Cell Biology 2019Quote: ... Proteins into rehydration buffer were incubated with an 18 cm immobilized pH 3–11 nonlinear gradient dry strip (GE Healthcare) for 16 hours at 20°C ...
-
bioRxiv - Molecular Biology 2019Quote: ... Unreacted biotin label was removed via SEC on a Superdex 200 Increase column (GE Healthcare Life Sciences). Biotinylated BSA-Lewis X was immobilized on streptavidin biosensors at 10 µg/mL ...
-
bioRxiv - Biophysics 2021Quote: ... Free biotin was removed from the samples using a desalting PD-10 column (GE Healthcare, Marlborough, MA).
-
bioRxiv - Biochemistry 2019Quote: ... resin and excess biotin was separated from the labeled protein by Superdex75 gel filtration chromatography (GE Healthcare) in 20 mM HEPES buffer pH 7.5 ...
-
bioRxiv - Systems Biology 2020Quote: ... The signal of biotin-bound HRP was visualized using Amersham ECL Western Blotting Detection Reagent (GE Healthcare).
-
bioRxiv - Biochemistry 2019Quote: ... Excess biotin was removed by size-exclusion chromatography on a S200 10/300 GL column (GE Healthcare) with a distal 1 ml GSTrap 4B (GE Healthcare) ...
-
bioRxiv - Microbiology 2023Quote: ... Biotin-labeled SARS CoV2 5’UTR RNA was immobilized on streptavidin-coated sensor chips (GE Healthcare Lifescience) to a final concentration of 300 Resonance Units (RU)/flow cell ...
-
bioRxiv - Molecular Biology 2020Quote: ... the clarified milk was thawed and loaded at 150 cm/h and 90 cm/h onto a 22 mL (1.6 x 11 cm) and 40 mL (1.6 x 20 cm) rPA Sepharose FF (GE Healthcare, Uppsala, Sweden) Protein A affinity chromatography column equilibrated with PBS for samples with a 25 mL and 100 mL milk starting volume ...
-
bioRxiv - Microbiology 2021Quote: PdeL samples (100 µl) varying from 11 to 371 µM were loaded onto a Superdex 200 (10/300) column (GE Healthcare) at constant flow (0.5 ml/min ...
-
bioRxiv - Neuroscience 2021Quote: ... Biotinylation was quenched and free biotins were separated using a homemade Sephadex G50 resin (GE Healthcare, Piscataway, NJ) column in TBS buffer ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins on nitrocellulose membranes were visualized with the biotin/streptavidin-horseradish peroxidase system (GE Healthcare; Little Chalfont, UK) in combination with the “Renaissance Western Blot Chemoluminescence Reagent” (Perkin Elmer ...
-
bioRxiv - Neuroscience 2020Quote: Derivatized-protein samples were analysed by two-dimensional (2D) gel electrophoresis using Immobiline® DryStrips pH 3-11 (IPG) non-linear (NL) 24 cm (GE Healthcare) for isoelectric focusing (IEF ...
-
bioRxiv - Neuroscience 2021Quote: ... The reaction was quenched and free biotins were separated using a homemade Sephadex G50 resin (GE Healthcare, Piscataway, NJ) column in TBS buffer (20 mM Tris pH 7.5 ...
-
bioRxiv - Bioengineering 2023Quote: An efficiency and SNR optimized variable-density 3D radial UTE sequence (11) with golden angle ordering was used on a 3T clinical scanner (Discovery MR750, GE Healthcare, Waukesha, WI) with an 8-channel cardiac phased-array coil (GE Healthcare ...
-
bioRxiv - Biochemistry 2022Quote: ... Proteins were purified from excess SNAP-biotin by size-exclusion chromatography on a Superdex 75 Increase 3.2/300 column using an ÅKTA micro system (GE Healthcare) in gel filtration buffer (20 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Cell Biology 2019Quote: ... Proteins were purified from excess SNAP-biotin by size-exclusion chromatography on a Superdex 75 Increase 3.2/300 column using an ÅKTAmicro system (GE Healthcare) in gel filtration buffer (20 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Capture of biotin-tagged nascent DNA was performed by incubating samples with streptavidin sepharose high performance (GE Healthcare Life Sciences) agarose beads for 16 h ...
-
bioRxiv - Biophysics 2022Quote: ... The biotinylated protein was separated from excess biotin on a Superdex 200 Increase 10/300 gel filtration column (GE Healthcare) equilibrated in 1x PBS buffer (10 mM Na2HPO4·7H2O ...
-
bioRxiv - Molecular Biology 2019Quote: ... cultures were grown in erythrocytes from which host leukocytes were removed by filtration through Whatman CF-11 cellulose (GE Healthcare Life Sciences; Pittsburgh, PA) [58] ...
-
bioRxiv - Cell Biology 2019Quote: ... Selected plakoglobin-containing fractions (Fig. 1d, fractions #8-11) were combined and loaded onto a 1 ml Resource Q anion exchange column (GE Healthcare Life Sciences, UK), equilibrated with 20 mM Tris pH 7.2 and 500 mM NaCl ...
-
bioRxiv - Molecular Biology 2023Quote: ... The Fc portion and uncut IgG was removed by flowing through a 1 mL or a 5 mL MabSelect SuRe column (GE Healthcare #11-0034-95) in PBS (Fab flows through the column) ...
-
bioRxiv - Bioengineering 2020Quote: ... Reactions were gently agitated at room temperature for 1 hour prior to removal of unreacted Biotin-NHS by size exclusion chromatography using PD-10 columns (GE Healthcare). Elution was performed using PBS ...
-
bioRxiv - Plant Biology 2020Quote: ... Desalting columns (NAP-5 and PD-10) and N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)proprionamide (HPDP-biotin) were purchased from GE Healthcare and Pierce ...
-
bioRxiv - Biophysics 2020Quote: ... 3’ biotinylated RNA constructs (linked via an extended TEG spacer arm) were purchased from Integrated DNA technologies (Baulkham Hills, NSW) and immobilised on a Biotin CAPture chip (GE Healthcare) in 10 mM sodium acetate pH 4.8 ...
-
bioRxiv - Immunology 2021Quote: ... Proteins were further purified and excess biotin removed from proteins biotinylated in vitro by size exclusion chromatography (Superdex 75 or 200 column [GE Healthcare]) in HBS-EP ...
-
bioRxiv - Immunology 2020Quote: ... Proteins were further purified and excess biotin removed from proteins biotinylated in vitro by size exclusion chromatography (Superdex 75 or 200 column [GE Healthcare]) in HBS-EP ...
-
bioRxiv - Biochemistry 2021Quote: ... The click-reaction was performed by incubation for 30 min at 20 °C in the dark with vigorous intermittent shaking and the free biotin was removed from the clicked extracts using PD MiniTrap G-25 column (GE Healthcare) equilibrated with IPD buffer ...
-
bioRxiv - Immunology 2020Quote: ... The antibodies were biotinylated using EZ-Link NHS-LC-LC-biotin (Pierce) and conjugated to one of several fluorophores - Cy5 Monoreactive dye (GE Healthcare), AlexaFluor 405 or AlexaFluor 647 NHS esters (ThermoFisher ...
-
bioRxiv - Immunology 2020Quote: ... Supernatants were first purified with affinity chromatography using AKTA pure 25 system and HiTrap MabSelect SuRe protein A column (GE healthcare, Cat# 11-0034-94) for huACE2-IgHu-pVax ...
-
bioRxiv - Biophysics 2019Quote: ... The unbound protein fraction containing biotin-PS-CFP2 was again purified via gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences). The monomeric biotin-PS-CFP2 was stored in 1 x PBS at −80 °C.
-
bioRxiv - Biophysics 2020Quote: ... AF555-conjugated I-Ek/MCC complexes (either C-terminally extended with 2xHis6 or biotin) were subjected to gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences) to remove an excess of unreacted MCC-AF555 ...
-
bioRxiv - Biophysics 2023Quote: ... Free biotin was removed using size-exclusion chromatography by loading the labeled protein on a Superose 6 gel-filtration column (GE Healthcare, Pittsburgh, PA) eluted with 20 mM HEPES pH 7.5 ...
-
bioRxiv - Neuroscience 2020Quote: ... antibody with respective secondary antibodies (1:5000, GE Healthcare).
-
bioRxiv - Molecular Biology 2020Quote: ... Soluble chromatin was pre-cleared by incubation with 10 μL of 50% RIPA-equilibrated Protein A + G sepharose bead slurry (GE Healthcare, Cat. No 17-5280-11 and 17-0618-06) for every 100 μL of chromatin for 1 h at 4°C ...
-
bioRxiv - Microbiology 2023Quote: ... Primary antibodies were detected with peroxidase-conjugated secondary antibodies (GE Healthcare).
-
bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were detected using horseradish peroxidase-conjugated secondary antibodies (GE Healthcare). To detect the deGRadFP expression from the hb-deGRadFP construct ...
-
bioRxiv - Biochemistry 2019Quote: ... via an Fc antibody using the Human Antibody Capture Kit (GE Healthcare). Immobilization levels ranged from 7,800-9,000 response units (RU ...
-
bioRxiv - Microbiology 2020Quote: ... antibody with HRP-conjugated anti-mouse secondary antibody (GE Healthcare or Promega). In addition to the experimental samples ...
-
bioRxiv - Pathology 2021Quote: ... horseradish peroxidase linked antibodies were used as a secondary antibody (GE Healthcare). Each signal was detected using Immunostar Zeta or LD (Fuji film ...
-
bioRxiv - Biochemistry 2021Quote: ... Secondary antibodies (GE Healthcare) were used at 1:5,000 for 1 h at room temperature ...
-
bioRxiv - Cell Biology 2019Quote: ... the primary antibody was visualized using 2~5 μg/ml secondary antibody towards specific species of primary antibody labeled with Cy3B (GE Healthcare) or Atto 488 (Sigma-Aldrich ...
-
bioRxiv - Physiology 2021Quote: ... Primary antibodies were detected with HRP-conjugated secondary antibodies (GE Healthcare, NA934, NA931), diluted 1:10,000 ...