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Citations for GE Life Sciences :
401 - 450 of 1572 citations for Caspase Recruitment Domain Family Member 17 CARD17 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Biotinylated proteins were isolated from cleared lysates by incubating with 70 µl of streptavidin beads (17-5113-01, GE Healthcare) at 4°C for 3h ...
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bioRxiv - Systems Biology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood of healthy donors by Ficoll-Paque (GE Healthcare, 17-1440-02) density gradient centrifugation ...
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bioRxiv - Cell Biology 2020Quote: ... The clear supernatant layer was collected and run over five serially connected Hi-Trap desalting columns (GE healthcare, 17-1408-01) in buffer A supplemented with 2 mM DTT and 14% glycerol ...
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bioRxiv - Cell Biology 2019Quote: ... Lysate was cleared with centrifugation and loaded to HisTrap FF crude 5 ml Ni-NTA column (GE healthcare, #17-5286-01). Column was washed with 10 column volume of lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was transferred to a tube containing 30 µl of pre-washed packed streptavidin beads (GE Healthcare, 17-5113-01). Streptavidin pulldown was done for 3 hours at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... 150 mM NaCl) and GST-fused proteins were immobilized on Glutathione Sepharose 4 Fast Flow beads (GE Healthcare, #17-5132-01) by incubating in a rotator at 4°C for 1 h ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM DTT) and addition of 40 µl Protein A Sepharose resin (nProtein A Sepharose 4 Fast Flow, 17-5280, GE Healthcare) that had been blocked for 1 h in ChIP Blocking buffer (ChIP Low salt buffer containing 2 mg/ml BSA and 2% (w/v ...
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bioRxiv - Genomics 2019Quote: ... Spheroplasts were lysed by resuspension in 20 ml cold F Buffer (18% w/v Ficoll-PM400 (GE Healthcare 17-0300-50), 40 mM potassium phosphate ...
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bioRxiv - Biophysics 2021Quote: ... the sample was filtered through a 0.22 μm filter and then purified by the Q column (GE Healthcare, 17-5156-01) and the Superdex 75 column (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... Fusion proteins were recovered from the cell lysates by conventional affinity chromatography on His-trapTMHP columns (GE Healthcare, 17-5247-01) using an Akta Explorer system (Amersham Pharm BiotechGE Healthcare).
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bioRxiv - Cancer Biology 2022Quote: ... cell supernatants/lysates were precleared using 30 μl of a 50% slurry of Protein G Sepharose (GE Healthcare, #17-0618-01), while for IP of pyo-KSR1 ...
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bioRxiv - Biophysics 2022Quote: ... Clarified lysate was passed through a 10 ml Ni2+-Sepharose 6 Fast flow resin (GE Healthcare Catalogue number 17-5318-02), washed with 50 ml of binding buffer (50mM Tris ...
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bioRxiv - Neuroscience 2020Quote: ... Soluble high-molecular weight seeding-competent tau (HMW SEC tau) was isolated from homogenate using size exclusion chromatography on a Superdex200 10/300GL column (#17-5175-01, GE Healthcare) as described previously(Takeda et al. ...
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bioRxiv - Microbiology 2021Quote: ... Lithium heparin-coated tubes were used for blood collection and plasma was isolated using Ficoll-Hypaque (GE Healthcare; 17-1440-03) in accordance with manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The purified protein was run over a 10/300 Superose 6 size exclusion column (GE Life Sciences, cat#17–5172–01). Fractions containing the trimeric F proteins were pooled and concentrated by centrifugation in a 50 kDa Amicon ultrafiltration unit (Millipore ...
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bioRxiv - Molecular Biology 2019Quote: ... SMCHD1 or γH2AX) and 30µL of preblocked Protein G Sepharose 4 Fast Flow 50% bead slurry (GE Healthcare, #17-0618-01) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Gels were dried and exposed to PhosphorImager screens overnight (approximately 17 hours) and imaged with a Typhoon-9410 scanner (GE Healthcare).
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bioRxiv - Developmental Biology 2019Quote: ... or 1 ng (for other mRNAs) of mRNAs in 1× modified Barths Solution (MBS) containing 4% Ficoll-400 (17-0300-05, GE Healthcare) using a microinjector (PLI-90 ...
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bioRxiv - Cell Biology 2020Quote: Secreted WNTs were enriched from cell culture supernatants using the affinity chromatography resin Blue Sepharose 6 Fast Flow (17-0948-01, GE Healthcare) and analysed by Western blotting according to previously published protocols (Glaeser et al. ...
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bioRxiv - Immunology 2020Quote: ... PBMCs from the patient samples and healthy donor were isolated by Ficoll gradient purification as per manufactures protocol (Ficoll, GE-17-1440, GE Healthcare). The isolated PBMC were stored by resuspending cells in cryopreservation media in aliquots of 5-10×106 cells per ml and storaed on liquid nitrogen.
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bioRxiv - Neuroscience 2022Quote: ... Samples were divided into two equal parts and added with 50 μl pre-equilibrated thiopropyl Sepharose 6B (Cat#17-0420-01, GE Healthcare) respectively ...
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bioRxiv - Neuroscience 2023Quote: ... and pellets were resuspended in 6 mL 25 % Standard Isotonic Percoll (Percoll: GE Healthcare, 17-5445-02 with 10% 10x PBS) in Medium A ...
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bioRxiv - Immunology 2023Quote: Peripheral blood mononuclear cells (PBMCs) were isolated by centrifugation using a Ficoll-Paque Plus gradient (GE Healthcare, Cat #17-1440-02), then washed by centrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... Labelling reactions proceeded for 40 mins at 4°C and the excess dye was removed using NAP-5 desalting columns (17-0853-01, GE Healthcare). Nanobodies to be used for confocal microscopy were labelled with Alexa488 (Thermo Fisher ...
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Higher meiotic chromosome condensation: a potential function of kinetochore through polo-like kinasebioRxiv - Cell Biology 2023Quote: ... Abcam)] overnight in rotating conditions followed by incubation with 50 µl of 50% Protein A Sepharose-beads (GE Healthcare,17-0780-01) for 2 hrs at 4°C in rotating condition ...
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bioRxiv - Microbiology 2023Quote: ... This suspension was slowly added to the top of 4 mLFicoll-Paque density gradient (GE Healthcare #17-1440-02, Uppsala, Sweden) and centrifuged for 40 minutes at 400 x g ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant GST-RAPSYN was purified from the supernatant by GST-affinity chromatography (SA010010, Smart-Lifescience) and size-exclusion chromatography (17-0060-01, GE Healthcare). Purified GST-RAPSYN protein was digested with thrombin (T8021 ...
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bioRxiv - Neuroscience 2021Quote: ... Free calcein or SST was removed by size-exclusion chromatography with Sephacryl® S-1000 column (Cat. # 17-0476-01, GE Healthcare). Second ...
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bioRxiv - Immunology 2022Quote: ... and resuspended in 24 mL of RT 70% PercollTM (1.088 g/mL at 22 °C; GE Healthcare Life Sciences 17-0891-01). Aliquots of 8 mL cell/PercollTM suspension were overlayed with 4 mL HBSS and centrifuged at 400 xg for 30 minutes RT ...
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bioRxiv - Biochemistry 2020Quote: ... The percent monomer was determined by dividing the estimated area under the monomer peak (elution volume of ∼15.4 to 17 mL) with the total area (monomer + dimer) using UNICORN software (default software of AKTA FPLC, GE Healthcare Life Science), followed by multiplication by 100.
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bioRxiv - Cell Biology 2021Quote: ... 500μl of dialyzed GST-Bsd or GST was bound to 50μl of PBS washed glutathione sepharose beads (GE Healthcare, 17-0756-01) and incubated at 4°C for 30min ...
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bioRxiv - Cell Biology 2021Quote: ... A volume of 2.5 ml of soluble fraction was incubated with 100 μl of Glutathione Sepharose beads (GE Healthcare, 17-0756-01) for 1 h at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Cleaved SPARC peptides were separated by 13.5% or 17% SDS PAGE and analyzed by immunoblotting or silver staining (GE Healthcare Life Sciences), respectively ...
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bioRxiv - Cell Biology 2022Quote: ... The peak fractions were pooled and loaded onto a Superdex 200 HiLoad 16/60 preparative-grade column (GE Healthcare, 17-1069-01) in 5 mL injections and developed at room temperature with the size exclusion buffer (20 mM Tris ...
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bioRxiv - Immunology 2020Quote: ... The supernatant was harvested 5 days post transfection and IgG was affinity purified using Protein A Sepharose (GE Healthcare, #17-5280-02). SDS-PAGE was used to confirm the purity of IgG and adequate cleavage of the P2A peptide ...
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bioRxiv - Plant Biology 2021Quote: ... and fractionated by gel permeation chromatography (GPC) using a Superdex 200 10/300 GL column (GE Healthcare, catalog no. 17-5175-01) connected to an AKTA FPLC system (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... We performed immunoprecipitations with precoated anti-SRSF9 or rabbit normal IgG-protein A Sepharose beads (GE Healthcare, catalog number: 17-1279-02) for 2 □ hours at 4□°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of protein from each sample were added to 50 µl of precleaned CaM binding beads (GE Healthcare, 17-0529-01) in 300 to 500 µl of 1% TX100-TNM lysis buffer and incubated with rotation at 4°C overnight (16-18 hours) ...
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bioRxiv - Biochemistry 2019Quote: ... 0.0027M KCl pH 8) and they were bound to a GST affinity column (GSTrap™ Fast Flow, GE Healthcare: 17-5131-02). The column was washed (0.02M NaPO4 ...
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bioRxiv - Developmental Biology 2019Quote: ... The mixture was layered on 40 ml of 5% Percoll (cat no. 17-0891-02, GE Healthcare Bio-sciences AB, Uppsala, Sweden) in HBSS (cat no ...
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bioRxiv - Neuroscience 2021Quote: ... human peripheral blood mononuclear cells were isolated from buffy coats obtained from healthy volunteers (Niguarda Hospital) after Ficoll®Paque Plus (Cat. 17-1440-02, GE Healthcare) density gradient centrifugation ...
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bioRxiv - Microbiology 2023Quote: Blood was drawn from five healthy donors and PBMCs were isolated using Ficoll-Paque Plus (17-1440-02, GE Healthcare, Danderyd, Sweden) gradient centrifugation ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were injected two times/embryo with mRNAs at two-cell or four-cell stage using a PicoSpritzer setup in 1/3x Modified Frog Ringer’s solution (MR) with 2.5% Ficoll PM 400 (GE Healthcare, #17-0300-50), and were transferred after injection into 1/3x MR containing Gentamycin ...
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bioRxiv - Developmental Biology 2023Quote: ... mRNAs or plasmid DNA at two-cell or four-cell stage using a PicoSpritzer setup in 1/3x Modified Frog Ringer’s solution (MR) with 2.5% Ficoll PM 400 (GE Healthcare, #17-0300-50), and were transferred after injection into 1/3x MR containing Gentamycin ...
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bioRxiv - Bioengineering 2023Quote: ... and the ends of the image were cropped flat to a length of 17 mm (MicroView ABA 2.1.1., GE Healthcare, London, Ontario, Canada). Past work has suggested that the use of a global segmentation threshold for a cohort with a broad range of bone volume fraction or varying bone mineral density can result in an inaccurate topology (Bouxsein et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 mM imidazole at pH 8) and loaded on a nickel affinity chromatography column (1 mL HisTrap, GE Healthcare, 17-5247-01). Bound proteins were eluted in elution buffer (20 mM NaH2PO4 ...
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bioRxiv - Cancer Biology 2024Quote: ... Peripheral blood and bone marrow mononuclear cells were isolated by density gradient centrifugation using the Ficoll® Paque Plus solution (17-1440-02, GE Healthcare).
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bioRxiv - Cancer Biology 2022Quote: Human peripheral blood mononuclear cells (PBMC) were purified from human blood buffy coat using Ficoll Paque Plus (GE Healthcare, cat. 17-1440-02) reagent and CD14+ microbeads (MACS Miltenyi Biotec ...
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Long-Range Electrostatic Interactions Significantly Modulate the Affinity of Dynein for MicrotubulesbioRxiv - Biophysics 2021Quote: ... and purified the MTBD constructs from the lysates using a HisTrap High Performance nickel Sepharose column (17-5247-01, Cytiva (formerly GE Healthcare Life Sciences), Marlborough ...
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bioRxiv - Cancer Biology 2020Quote: ... to separate blood constituents and isolation of buffy coat density gradient centrifugation (400 × g at 18°C for 30 min) of RPMI-1640 diluted blood over a Ficoll-Paque(tm) Plus reagent (Cat. 17-1440-02, GE Healthcare, Piscataway, NJ) was performed ...