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Citations for GE Life Sciences :
1 - 50 of 2987 citations for Calcium calmodulin Dependent 3' 5' Cyclic Nucleotide Phosphodiesterase 1C PDE1C Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The eluate thus obtained was incubated with equilibrated 200ul Calmodulin beads (Calmodulin SepharoseTM 4B from GE Healthcare) in Calmodulin Binding Buffer (10mM Tris-HCl pH=7.5,150mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... After elution from Calmodulin Sepharose 4B (GE healthcare), the eluate was applied to a MonoQ 5/50 GL column (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads are subjected to TEV cleavage as described previously followed by incubation with 600ul pre-equilibrated Calmodulin beads (Calmodulin SepharoseTM 4B from GE Healthcare) in 3ml Calmodulin Binding Buffer by slow rotation on a rotator wheel overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: About 300 μl of Calmodulin-Sepharose 4B (GE healthcare) beads suspension was equilibrated with 10 ml of calmodulin-binding buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... Excess unbound nucleotide was removed using a NAP-5 column (GE Healthcare, 17085302).
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bioRxiv - Cell Biology 2020Quote: ... the supernatant was mixed with calmodulin-sepharose resin (GE Healthcare) for 90 min at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... the supernatant was mixed with calmodulin-sepharose resin (GE Healthcare) for 90 min at 4°C ...
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bioRxiv - Physiology 2020Quote: ... After washing calmodulin and control Sepharose 4B beads (GE Healthcare) twice with lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Cleaved protein was collected and applied to calmodulin sepharose (GE Healthcare) pre-washed with binding buffer (10 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... 0.1% Triton X-100) Calmodulin Sepharose (GE Healthcare, 17-0529-01) and incubated for 3h at 4°C with gentle rotation. ...
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bioRxiv - Biochemistry 2020Quote: ... the 5’ half of the tRNA (nucleotides 1-39) was chemically synthesized (GE Healthcare Dharmacon) and enzymatically ligated to the chemically synthesized 3’ half following established protocols (52 ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant of yeast lysate was incubated with calmodulin resin (GE Healthcare) overnight at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant of yeast lysate was incubated with calmodulin resin (GE Healthcare) overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... and purified by fast protein liquid chromatography using Calmodulin-Sepharose (GE Healthcare, USA). TAT-CaM was expressed in BL21(DE3)pLysS E ...
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bioRxiv - Biochemistry 2019Quote: ... Nucleotide composition was analyzed as described above on Mono Q® 5/50 GL columns (GE Healthcare) using an FPLC system (monitoring 254 nm absorbance) ...
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bioRxiv - Cell Biology 2021Quote: ... IgG purified protein was further subjected to affinity purification using Calmodulin Sepharose (GE Healthcare). Purified TRAPPII was further subjected to size exclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluate was incubated with 0.3 ml of Calmodulin Sepharose 4B beads (GE Healthcare) in the addition of 7 ml of Calmodulin binding buffer (10 mM Tris-Cl pH 8.0 ...
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bioRxiv - Biochemistry 2019Quote: Radioactive nucleotides were from Perkin Elmer and unlabeled nucleotides were from GE Healthcare. DNA modification enzymes were from New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... Calmodulin and EEN1 were purified on a Superdex-200 16/600 column (GE Healthcare, U.S.A) in solution buffer containing 20 mM Tris-Cl at pH 7.0 ...
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bioRxiv - Biochemistry 2020Quote: ASLPro containing a m1G37 modified anticodon stem-loop (18 nucleotides) and mRNA (19 nucleotides) were purchased from GE Healthcare Dharmacon and dissolved in 10 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2024Quote: ... The eluates were pooled and incubated with 50 µL pre-equilibrated calmodulin sepharose 4B (GE Healthcare) for 1 h at 4 °C while rotating on a wheel ...
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bioRxiv - Neuroscience 2020Quote: ... Binding was detected with an anti-mouse Cy-3 and anti-rabbit Cy-5 antibodies (GE Healthcare). Blots were scanned using a Typhoon 5 (GE Healthcare ...
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bioRxiv - Neuroscience 2019Quote: ... Each probe contains a 3’-end amine-modified nucleotide that allows directly couple to an NHS-ester Cy3 dye (GE Healthcare, PA23001) according to the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... CG42797EcoRI_F: 5’-CCGgaattcATGGAGCCACCAGCT-3’ and CG42797XhoI_R: 5’-TAGAGGTACCctcgagCTACTCAATGCCGAACGTGTTG-3’ and cloned by enzymatic digestion into a pGEX6P1(GE Healthcare).
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... CG42797EcoRI WWI_F: 5’-ccgGAATTCCCACCATTGCCGCCTG-3’ CG42797XhoI WWII_R: 5’-ccgCTCGAGTCAACGAGGATCCATGAA-3’ and cloned by enzymatic digestion into a pGEX6P1(GE Healthcare).
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... CG42797EcoRI_F: 5’-CCGgaattcATGGAGCCACCAGCT-3’ and CG42797XhoI_Nterm_R: 5’-CCGctcgagTCATTCGCTGGGCTGC-3’ and cloned by enzymatic digestion into a pGEX6P1(GE Healthcare).
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bioRxiv - Molecular Biology 2019Quote: ... The reaction was heat-inactivated for 5 min at 95°C and the free nucleotides were removed on a Sephadex G-25 column (GE Healthcare). The oligo was annealed to a 1.5x excess of the unlabelled complementary strand by heating for 5 min to 80°C and slowly cooling to RT in annealing buffer (50 mM Hepes ...
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bioRxiv - Cell Biology 2020Quote: ... was amplified by PCR using the primers (5’-GGTTCCGCGTGGATCCATGTCTCATGCAGCCGAGCCA-3’ and 5’-GGAATTCCGGGGATCCTCAGGACTCCTCTTCAATGCTGA-3’) and cloned into BamHI site of pGEX-4T-1 expression vector (GE Healthcare) using In-Fusion HD Cloning System (Clontech) ...
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bioRxiv - Cancer Biology 2022Quote: Custom siRNAs targeting 5’-CACCAUGAGUGGCAGUCAG-3’ 33 for FRA1 and 5′-GUUCACUGCUGGCCUAUAA-3’ 34 for FLI1 were ordered from GE Healthcare Dharmacon ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were visualised on an Odyssey CLx Imaging System (LiCOR) (Figure 1C) or an ImageQuant LAS 4000 (GE Healthcare) with SuperSignal West Pico PLUS HRP substrate (Thermo Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... and purified from free nucleotides through G25 spin columns (GE Healthcare). Binding was carried out by incubating 0.5 ng of labelled DNA with 1 μg of unmodified or PARP1 covalently PARylated TRF1 (as above described ...
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bioRxiv - Systems Biology 2019Quote: ... Free nucleotide was removed using G-25 MicroSpin columns (GE Healthcare). Oligonucleotides were subsequently heated to 75°C and slowly cooled to room temperature to allow proper annealing ...
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bioRxiv - Cell Biology 2023Quote: ... and unincorporated nucleotide removed with Illustra G-50 columns (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... The kinase complex was isolated using IgG sepharose 6 Fast Flow and Calmodulin sepharose 4B affinity resin (both GE Healthcare). In the last step of all bacterially expressed proteins ...
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bioRxiv - Microbiology 2022Quote: ... The TEV protease treated suspension was then transferred into the new columns containing 500 μl Calmodulin Sepharose 4B (GE Healthcare) and 6 ml of CBB buffer [150 mM NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3–5 times and incubated with a 1:5000 dilution of horseradish peroxidase-conjugated goat anti-rabbit antibody (GE Healthcare, UK) for one hour ...
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bioRxiv - Cell Biology 2020Quote: ... Nucleotides were removed with a Heparin column (HiTrap Heparin HP, GE Healthcare) by binding protein in 250mM NaCl and isocratic elution in 750mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... Unincorporated nucleotides were removed by using MicroSpin G-25 Columns (GE Healthcare). For the enzymatic reactions ...
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bioRxiv - Biophysics 2019Quote: ... Nucleotides were removed with a Heparin column (HiTrap Heparin HP, GE Healthcare) by binding protein in 250mM NaCl and isocratic elution in 750mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... Unincorporated nucleotides were removed using ProbeQuant G-50 Micro Columns (GE Healthcare). The radiolabeled probes were heat denatured for 5 min at 100°C immediately prior to hybridization to the membrane.
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bioRxiv - Molecular Biology 2024Quote: ... Unincorporated nucleotides were removed using ProbeQuant G-50 Micro Columns (GE Healthcare). The radiolabeled probes were heat denatured by incubating for 5 min at 100°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Unincorporated nucleotides were removed using ProbeQuant G-50 Micro Columns (GE Healthcare). The radiolabeled probes were heat-denatured for 5 min at 100°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Unincorporated nucleotides were removed using ProbeQuant G-50 Micro Columns (GE Healthcare). The radiolabeled probes were heat denatured by incubating for 5 min at 100°C immediately prior to hybridization to the membrane.
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bioRxiv - Molecular Biology 2021Quote: ... The intensities of the bands of SREBP-1c and Lamin A/C in nuclear extracts were quantified with Image analysis software (ImageQuant TL, GE Healthcare). The intensities of nuclear SREBP-1c were normalized by those of corresponding Lamin A/C intensity.
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bioRxiv - Molecular Biology 2019Quote: ... unincorporated nucleotides were removed using MicroSpin G-25 columns (GE Healthcare Life Sciences).
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bioRxiv - Biochemistry 2020Quote: ... Unincorporated nucleotides were then removed using Illustra MicroSpin G-50 columns (GE Healthcare) and the DNA isolated via phenol-chloroform extraction with Phase Lock Gel Tubes as per the manufacturer’s instructions (5PRIME) ...
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bioRxiv - Biochemistry 2019Quote: ... The excess nucleotide was removed by buffer exchange using NAP5 columns (GE healthcare) or size-exclusion chromatography columns (Superdex 75 10/300 ...
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bioRxiv - Biochemistry 2022Quote: ... Free nucleotides were removed from the RNA using Sephadex G25 resin (GE Healthcare) and RNA integrity and purity was verified by denaturing urea-polyacrylamide gel electrophoresis ...
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bioRxiv - Molecular Biology 2023Quote: ... and unincorporated nucleotides were removed using ProbeQuant G-50 Micro Columns (GE Healthcare) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... and unincorporated nucleotides were removed using ProbeQuant G-50 Micro Columns (GE Healthcare) according to the manufacturer’s instructions.