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Citations for GE Life Sciences :
401 - 450 of 2151 citations for CEACAM 5 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... the bacterially expressed 6×His tagged ARL3 and its variants were purified with Ni SepharoseTM 6 Fast Flow beads (GE Healthcare) and cleaved with thrombin (Solarbio ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared via ultracentrifugation and his-tagged protein purified on the AKTÄ Pure on the HisTrap HP column (GE Healthcare). Fractions of interest were second round purified via size exclusion chromatography on the Superdex 200 column (GE Healthcare) ...
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bioRxiv - Plant Biology 2020Quote: ... The His-MBP tags were cleaved by TEV protease overnight and removed by flowing through a HisTrap FF column (GE Healthcare) again ...
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bioRxiv - Plant Biology 2020Quote: ... Anti-NPR1 antibodies were purified from antisera by affinity chromatography using a tNPR1-His-immobilized column (Hitrap NHS-activated HP; GE Healthcare). To prepare for the tNPR1-His-immobilized column ...
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bioRxiv - Microbiology 2020Quote: ... Purification of the protein from the soluble fraction was done in Binding buffer on a 1 mL His Trap Column (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The elution fractions were concentrated and (His)6-Rv3341 was further purified by gel exclusion chromatography using Superdex 200 Increase 10/300 GL column (GE Healthcare). The purified fractions were pooled ...
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bioRxiv - Microbiology 2020Quote: ... Solubilized IBs were purified by immobilized metal affinity chromatography (IMAC) by loading onto a prepacked 1 ml His-Trap HP column (GE Healthcare) equilibrated with lysis buffer containing 8M urea at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare) ...
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bioRxiv - Biophysics 2020Quote: ... Immunoblots with anti-HA, anti-His or anti-SecB antibodies were visualized by Lumino image analyzer LAS-4000 mini (Cytiva, Tokyo, Japan (GE Healthcare)) using ECL Prime Western Blotting Detection Reagents (Cytiva ...
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bioRxiv - Cell Biology 2020Quote: ... Fusion proteins were recovered from the cell lysates by conventional affinity chromatography on His-trapTMHP columns (GE Healthcare, 17-5247-01) using an Akta Explorer system (Amersham Pharm BiotechGE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... Size exclusion chromatography was performed to separate FLAG-Ddi1 from the cleaved SUMO protein or the full-length His-SUMO-FLAG-Ddi1 using a HiLoad 26/600 Superdex 200 pg column (GE Healthcare) in a buffer of 20 mM Tris HCl pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: Kinetic interactions between the antibodies and his-tagged antigen proteins were measured at room temperature using Biacore T200 surface plasmon resonance (GE Healthcare). Anti-human fragment crystallizable region (Fc region ...
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bioRxiv - Biophysics 2022Quote: ... with an N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was expressed using Hi5 cells and purified by Ni-NTA resin and gel filtration chromatography (GE Healthcare) in HBS buffer (10 mM HEPES ...
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bioRxiv - Developmental Biology 2022Quote: ... The precipitants were dialyzed against a 400 times volume of PBS (-) plus Triton X-100 and then applied to a Nickel (Ni)-Sepharose Histidine-affinity column (His GraviTrap; GE Healthcare). Bound proteins were eluted with PBS (- ...
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bioRxiv - Biophysics 2022Quote: ... The clarified supernatant was purified with the His-tag affinity by chromatography with a HisTrap HP Ni2+sepharose column (GE Healthcare). The collected peak fraction was further purified through a HiTrap desalting column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... RAD51 without the his-SUMO tag was then recovered into the flow thru and directly loaded onto a HiTrap heparin column (GE Healthcare). The column was washed with W2 buffer and then a 0.1-1M NaCl gradient was applied ...
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bioRxiv - Bioengineering 2022Quote: ... His-tagged Ri-boswitch-VLPs were purified using a Ni beads gravity column loaded with high-performance Ni beads (GE Healthcare). Briefly ...
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bioRxiv - Biochemistry 2022Quote: (His)6-GST-CAPN7(MIT)2 was bound to GST-sepharose beads (10 mL,GE Healthcare Life Sciences, USA, 6 h), washed with 1 L wash buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Immobilized metal ion affinity chromatography of the His-tagged Pdr5 was performed with a Zn2+ loaded 1 ml HiTrap Chelating column (GE Healthcare) and elution was carried out with a step gradient using low and high histidine buffers (50 mM Tris-HCl pH 7.8 ...
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bioRxiv - Molecular Biology 2019Quote: ... (amino acids 2-241 fused to 6 x his) were expressed in BL21 cells and purified on glutathione-linked sepharose beads (GE healthcare) or Ni-NTA-Agarose (GE healthcare ...
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bioRxiv - Microbiology 2021Quote: Purified His-tagged Pentamer was immobilized to a single flow cell of a NiNTA sensor in a Biacore X100 (GE Healthcare) to a level of ∼800 response units (RUs ...
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bioRxiv - Plant Biology 2021Quote: ... the eluates containing recombinant proteins with 10× His-MsyB tag were further purified by an anion-exchange column (Source-15Q; GE Healthcare). The 10×His-MsyB tag was cleaved by TEV protease at 4 °C overnight and removed by an anion-exchange column ...
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bioRxiv - Immunology 2021Quote: The scCl5 was purified using a two-steps protocol using first immobilized metal affinity chromatography (IMAC) to enrich for His tagged protein (1 ml HisTrap, GE Healthcare) followed by anion exchange (1 ml HiTrapQ ...
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bioRxiv - Microbiology 2021Quote: ... HisTRAP eluted peak fractions were pooled and applied to a Hi-Load 16/600 Superdex-200 (preparation grade) size-exclusion column (GE Healthcare) equilibrated with the gel filtration buffer (50 mM Tris-HCl ...
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bioRxiv - Immunology 2021Quote: ... The peak fractions were collected and purified by size exclusion chromatography using Hi-Load 16/60 Superdex-200 (prep-grade) gel filtration column (GE Healthcare) in a buffer containing 25 mM Tris·HCl (pH 8.0 ...
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bioRxiv - Immunology 2021Quote: ... Peak protein fractions were pooled and loaded onto the size-exclusion chromatography column using Hi-Load 16/60 Superdex-200 (prep-grade) gel filtration column (GE Healthcare) in a buffer containing 25 mM Tris·HCl (pH 8.0 ...
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bioRxiv - Molecular Biology 2022Quote: 1.5 mg of pure PfP2 incubated at 37°C for 3h and was subsequently loaded onto a Hi Load Superdex 75 16/600 gel filtration column (GE Healthcare) pre-equilibrated with 20mM Tris-HCl (pH 7.4 ...
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bioRxiv - Immunology 2020Quote: ... Purification of His-HMGB1 from 293-freestyle cell lysate was carried out using Ni SepharoseTM 6 Fast Flow gel (GE Healthcare). After purification ...
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bioRxiv - Biochemistry 2020Quote: ... The liberated affinity-tag and the His-tagged Senp1 protease were removed by a second round of affinity purification with HisTrap FF columns (GE Healthcare). All proteins were purified with a final size exclusion chromatography (HiLoad 16/600 Superdex 75 or 200 pg ...
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bioRxiv - Microbiology 2020Quote: ... Lysed cells were ultracentrifuged and the cleared supernatant loaded onto a Ni Sepharose 6 Fast Flow His Trap column (GE Healthcare) equilibrated with Buffer A ...
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bioRxiv - Molecular Biology 2019Quote: ... Solubilized IBs were purified by immobilized metal affinity chromatography (IMAC) by loading onto a prepacked 1 ml His-Trap HP column (GE Healthcare) equilibrated with lysis buffer containing 8M urea at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... The nanobodies were fused with a His-tag at C-terminus and purified over Ni Sepharose 6 Fast Flow (GE Healthcare) and eluted with 400 mM imidazole ...
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bioRxiv - Molecular Biology 2021Quote: ... with an anti-his-tag mAb (MBL) as the primary and anti-mouse IgG HRP linked whole Ab from sheep (GE Healthcare) as the secondary antibody ...
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bioRxiv - Biophysics 2021Quote: ... was purified by 1 M NaCl containing Tris buffer using AKTATM Pure protein purification system with Hi-Trap Q-FF anion exchange column (GE healthcare). 0.3% H2O2 was added to the purified proteins ...
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bioRxiv - Biophysics 2020Quote: ... Bacterial biomass was harvested by centrifugation at 5,400g and His-tagged nanobodies were purified using HisTrap™ column chromatography (GE Healthcare) followed by a buffer exchange step to PBS using a HiTrap™ desalting column (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: ... Cell lysates were centrifuged at 12,000 x g for 30 min and the supernatant was subsequently used for protein purification through a HisTrap column (His- tagged full-length proteins, GE Healthcare) or Amylose resin (MBP-tagged fragments ...
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bioRxiv - Immunology 2021Quote: ... to prevent activation of the alternative complement pathway and complement consumption) and anti-FH mAb (35H9) on Hi-Trap HP-NHS columns (GE Healthcare). FH protein was purified from EDTA-plasma using an anti-FH mAb (35H9 ...
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bioRxiv - Biochemistry 2021Quote: ... The flow-through containing nsp12 was further purified by ion exchange chromatography using Hi TRAP Q HP and SP HP pre-packed columns (GE Healthcare) equilibrated with buffer D ...
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bioRxiv - Microbiology 2022Quote: ... The protein was further purified by Ni-NTA immobilized metal-affinity chromatography (IMAC) using five milliliter His-Trap HP columns (GE Healthcare), followed by size exclusion chromatography (SEC ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tagged sample was purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose Excel resin (GE Healthcare Life Sciences). The IMAC eluted sample was dialyzed and treated with His-tagged Human Rhinovirus 3C protease as described previously32 ...
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bioRxiv - Microbiology 2022Quote: ... Size exclusion chromatography was performed in 20 °C ÄKTA purifier system (Amersham) and a prepacked Hi-Prep 16/60 Sephacryl S-200 high-resolution column (GE Healthcare). Flow rate was 0.5 ml/min ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tagged sample was purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose Excel resin (GE Healthcare Life Sciences). The IMAC eluted sample was dialyzed and treated with His-tagged Human Rhinovirus 3C protease ...
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bioRxiv - Microbiology 2023Quote: ... Lysate was cleared by centrifugation and the supernatant loaded into an anion exchange column Hi-Trap Sepharose Q-HP (GE Healthcare). KtrA and KtrC were eluted with a 50-1000 mM KCl gradient ...
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bioRxiv - Plant Biology 2022Quote: ... radioactive signals from His-PIN1L were detected on the dried gel by the Typhoon imaging system (GE Healthcare TYPHOON FLA 9500).
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bioRxiv - Systems Biology 2023Quote: ... The lysates were clarified by centrifugation for 1h at 4ºC at 12000 rpm and loaded on a His GraviTrap™ column (GE Healthcare). After washing with 10 mL of washing buffer (40 mM sodium phosphate ...
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bioRxiv - Molecular Biology 2023Quote: ... The fusion protein was eluted using binding buffer with 250 mM imidazole and then further purified using size-exclusion chromatography on a Hi load 16/60 Sephacryl S- 100 column (GE Healthcare) in the binding buffer with pH adjusted to 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... To remove any residual impurities final step of size-exclusion chromatography on a Hi load 16/60 Sephacryl S-100 column (GE Healthcare) in the binding buffer with pH adjusted to 7.4 was employed ...
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bioRxiv - Biochemistry 2023Quote: Protein purification included two steps of immobilized metal-affinity chromatography separated by a thrombin-catalyzed His-tag cleavage and thrombin removal on a heparin column (GE Healthcare), hydrophobic interaction chromatography (HIC ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was collected and used for recombinant protein purification either by using AKTA FPLC or on His-Trap columns (GE Healthcare) as per the manufacturer guidelines or by manually purifying pre-equilibrated Ni-NTA-agarose (Qiagen) ...
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bioRxiv - Molecular Biology 2023Quote: ... Column was washed with binding buffer and the proteins of interest were eluted with the binding buffer supplemented with 250 mM imidazole and applied to size exclusion chromatography column Hi load 16/60 Sephacryl S-100 column (GE Healthcare). Fractions of interest were pooled ...
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bioRxiv - Microbiology 2023Quote: ... Size exclusion chromatography was performed in 20°C ÄKTA purifier system (Amersham) and a prepacked Hi-Prep 16/60 Sephacryl S-200 high-resolution column (GE Healthcare). Flow rate was 0.5 ml/min ...