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Citations for GE Life Sciences :
201 - 250 of 1336 citations for CD122 IL 2RB Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... the His-tagged DnaK protein was purified using a HisTrap HP column (GE Healthcare) with 2×PBS buffer followed by further purification using a HiTrap Q column (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... The purified protein was desalted using a His Trap Desalting columns from GE Healthcare according to the prescribed protocol and was stored at −80°C with 40 % glycerol.
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were purified over 1-2 ml His-Trap FF columns (GE Healthcare) with elution buffer containing 250 mM Imidazole ...
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bioRxiv - Plant Biology 2022Quote: ... The His-MDIS1CD protein is loaded onto a Ni-NTA agarose column (GE Healthcare) and eluted using elution buffer containing 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were applied to Nickel-Nitrilotriacetic (Ni-NTA) His-Trap FF column (GE Healthcare) and 6xHis-tagged-mCherry-p62 was eluted via a stepwise imidazole gradient (50 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... GgUox was purified using a 5 mL His-trap Ni-NTA column (GE Healthcare) equilibrated in 50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Biophysics 2023Quote: ... loaded onto a Hi-Trap heparin affinity column (2×5 ml, GE Life Sciences), and eluted with a salt gradient (200–800 mM NaCl) ...
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bioRxiv - Biophysics 2023Quote: ... and applied to a Hi Trap SP HP column (GE Healthcare #17-1151-01) equilibrated with dCas9 dialysis buffer ...
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bioRxiv - Biophysics 2023Quote: ... and applied to a Hi Trap SP HP column (GE Healthcare #17-1151-01) equilibrated with dCas9-Cys dialysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was passed through a His Prep FF 16/10 column (GE Healthcare) and washed with 5 column volumes of buffer ...
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bioRxiv - Biophysics 2023Quote: ... Subsequent removal of DTT was achieved with a Hi-Trap desalting column (GE Healthcare) equilibrated in buffer A without β-mercaptoethanol ...
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bioRxiv - Biochemistry 2024Quote: ... ZapD and His-Smt3 were separated by chromatography in 5-ml HisTrap (GE Healthcare), then the protein was eluted in a 5-ml HiTrap Desalting column (Healtcare ...
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bioRxiv - Biochemistry 2024Quote: ... coli as His-tag proteins and were purified using the IMAC principle (GE healthcare). Following purification ...
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bioRxiv - Synthetic Biology 2021Quote: ... then imaged using a DeltaVision microscope (GE Healthcare, Chicago, IL) equipped with a 100x objective (Olympus ...
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bioRxiv - Evolutionary Biology 2021Quote: ... quantified with a NanoVue spectrophotometer (GE Healthcare, Chicago, IL, USA), and stored at −80°C ...
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bioRxiv - Systems Biology 2020Quote: ... blots were flooded with ECL (Amersham; GE Healthcare, Chicago, IL) and bands detected using chemiluminescent film (Hyperfilm ECL ...
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bioRxiv - Microbiology 2019Quote: ... Superose 6 10/300 GL (GE Healthcare, Chicago, IL, USA) equilibrated with 10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... and a Superdex 200 size exclusion chromatography (GE Healthcare, IL). The peak fractions were concentrated to 0.1 mg/mL for DXT (Fig ...
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bioRxiv - Cell Biology 2021Quote: ... and Superdex 75 gel filtration chromatography (GE Healthcare, Chicago, IL) into 20 mM HEPES buffer pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... M13KO7 helper phage were obtained from GE Healthcare (Chicago, IL). Ampicillin ...
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bioRxiv - Biochemistry 2021Quote: ... gels were soaked in Amplify solution (GE Healthcare, Chicago, IL) for 30 min before drying on a vacuum air dryer ...
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bioRxiv - Biochemistry 2020Quote: ... 0.5 ml Sephadex G-50 (GE Healthcare Life Sciences, IL) in order to remove excess NHS-fluorescein.
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bioRxiv - Neuroscience 2019Quote: ... 5% horse serum (HS; GE Healthcare Life Sciences, Chicago, IL), 1% Pen-Strep (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Supernatants were incubated with Glutathione Sepharose (GE Healthcare, Chicago, Il) for 90 minutes after which beads were washed with IPB ...
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bioRxiv - Cancer Biology 2020Quote: ... and 1% Penicillin/Streptomycin (#sv30010, HyClone, GE Healthcare, Chicago, IL). The mantle cell lymphoma cell line Maver-1 (#ACC 717 ...
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bioRxiv - Neuroscience 2022Quote: A 3.0T MRI scanner (GE 750; GE Healthcare, Chicago, IL) with equal imaging protocol settings at all time points was applied to acquire axial head 3-dimensional T1-weighted ...
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bioRxiv - Molecular Biology 2019Quote: ... and imager ImageQuant LAS 4000 (GE Healthcare; Chicago, IL, USA). Primary and secondary antibodies used in this study are summarized in Table 3.
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bioRxiv - Plant Biology 2020Quote: ... Horseradish peroxidase –conjugated secondary antibody (GE Healthcare, Chicago, IL, USA) and Amersham ECL (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... proteins were visualized using ECL reagent (GE Healthcare, Chicago, IL) and immunoblot intensities were quantified with ImageJ.[32].
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bioRxiv - Plant Biology 2019Quote: ... filled with Sephadex G-50 superfine (GE Healthcare, Chicago, IL) was run on a Bio-Rad BioLogic Duo Flow FPLC system equipped with a QuadTec detector ...
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bioRxiv - Cancer Biology 2020Quote: ... and immobilized onto nitrocellulose membranes (GE Healthcare, Chicago, IL, USA) by electroblot (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2021Quote: ... visualized using ImageQuant LAS 500 (GE Healthcare, Chicago, IL, US) then quantified relatively to loading controls by optical density using ImageJ (V1.46d ...
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bioRxiv - Biochemistry 2021Quote: ... Gels were soaked in Amplify (GE Healthcare, Chicago, IL, USA), dried and exposed to films (Carestream Health Medical X-ray Blue ...
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bioRxiv - Physiology 2020Quote: ... or ECL™ Prime reagent kits (GE Healthcare, Chicago, IL). Western blots were first probed with the antibody of interest ...
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bioRxiv - Microbiology 2019Quote: ... and exposed to a phosphor screen (GE Healthcare, Chicago, IL) overnight ...
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bioRxiv - Plant Biology 2020Quote: ... and purified using Glutathione Sepharose 4B resin (GE healthcare, IL). Both MBP-tagged and GST-tagged recombinant proteins were further purified with a Nanosep® 30-kDa size-exclusion column (PALL ...
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bioRxiv - Plant Biology 2020Quote: ... coli BL21 (DE3) using pGEX4T-3 vector (GE healthcare, IL).
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bioRxiv - Microbiology 2020Quote: ... or anti-rabbit Ig-HRP (GE Healthcare, Chicago, IL, USA) both diluted to 1:5,000 ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubated in streptavidin-Cy5 (GE Healthcare, Chicago, IL, USA) [16] ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were isolated with Ficoll-Hypaque (GE Healthcare, Chicago, IL) density gradient centrifugation ...
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bioRxiv - Cell Biology 2024Quote: ... 10% fetal bovine serum (FBS) (GE Healthcare, Chicago, IL, USA), and a mixture of 100 units/ml penicillin and 100 μg/ml of streptomycin (P/S ...
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bioRxiv - Plant Biology 2024Quote: ... Horseradish peroxidase-conjugated secondary antibody (GE Healthcare, Chicago, IL, USA) and Amersham ECL (GE Healthcare ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies were obtained from GE Healthcare (Chicago, IL, USA). Bound antibodies were detected by ECL (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... was cloned into mouse IgG1 or human IgG1 Fc backbone in pKN293E expression vectors and transiently transfected into HEK293 cells followed by media collection and purification using MabSelect SuRe antibody purification resin (Cat: 29-0491-04, GE Healthcare). SEC-HPLC and SDS-PAGE were used to check the size and purity of these recombinant proteins.
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bioRxiv - Biochemistry 2020Quote: The recombinant catalytic domains of MMP-2 and -9 were expressed in HEK293 cells stably transfected with respective constructs and purified from serum-free culture medium using Gelatin Sepharose 4B (GE Healthcare) as described in [35 ...
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bioRxiv - Immunology 2020Quote: IgGs were expressed by transient transfection in HEK293-6E cells and purified from cell supernatants using protein A or G (GE Healthcare) as previously described (39 ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were purified by Ni-affinity chromatography on 5 mL His-Trap columns (GE Healthcare) as follows ...
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bioRxiv - Molecular Biology 2021Quote: 1ug of soluble His-Rap1 was pre-cleared on Glutathione sepharose 4B beads (GE Healthcare) for 1h at 4C ...
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bioRxiv - Biochemistry 2019Quote: ... The recombinant protein was purified under denaturing conditions over a His-SpinTrap column (GE Healthcare), desalted using a PD SpinTrap G-25 column (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... The His-tagged UBC13 and its variants were bound to HisTrap HP columns (GE Healthcare) and were eluted with 100mM-500mM Imidazole gradient ...