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Citations for GE Life Sciences :
301 - 350 of 3998 citations for Borrelia burgdorferi sensu stricto B31 ErpN OspE Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... Protein content were estimate using 2D QuantKit (GE Healthcare) and adjusted to 2 µg/µl ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were detected on a LAS-4000 (GE Healthcare) in SYBR-green fluorescence detection mode and Image Quant TL software.
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bioRxiv - Plant Biology 2020Quote: ... the cells were lysed by sonication and the fusion protein was purified using HisTrap HP histidine-tagged protein purification column of AKTA pure FPLC system (GE Healthcare, Pittsburgh, PA). The purified protein was further dialyzed overnight and further purified with HiLoad 16/600 Superdex 200 pg column (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... The proteins were harvested from the supernatant of cell cultures and purified by HisTrap Excel (for his-tagged proteins) or Protein G (for antibodies) columns (GE Healthcare Life Sciences). RHMS-1 (trimer) ...
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bioRxiv - Microbiology 2021Quote: ... Cleavage of the GST tag to induce cleaved fusion proteins was performed using PreScission protease (10 U/mg of fusion protein, GE Healthcare, Uppsala, Sweden) or thrombin protease (10 U/mg of fusion protein ...
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bioRxiv - Biochemistry 2020Quote: ... This cell supernatant was then loaded into protein A affinity column (HiTrap protein A high performance, GE29-0485-76; GE Healthcare Life Sciences,) using liquid chromatography system (GE AKTA Prime Plus) ...
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bioRxiv - Microbiology 2022Quote: ... Protein-protein interaction and protein complex sizes were estimated by comparing the stained protein bands with the native protein molecular weight markers range between 60 to 690 kDa (Amersham, GE Healthcare Life Sciences). Protein bands excised from the stained native protein gels were identified by MS analysis.
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bioRxiv - Molecular Biology 2023Quote: ... The MBP-Q44-HttEx1 protein was purified by fast protein liquid chromatography (FPLC) using a 5 ml HisTrap HP nickel column (GE Healthcare, Uppsala, Sweden) with 0.5 M imidazole gradient (SKU I5513-100G ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant proteins were further purified by gel filtration on an ÄKTA Pure fast protein liquid chromatography (FPLC) system (GE Healthcare Life Sciences). The principal peaks were collected ...
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bioRxiv - Neuroscience 2020Quote: ... in which they were precleared for 1 h using 30 μl (dry volume) of a 1:1 mixture of protein-A and protein-G-agarose beads (GE Healthcare, Chicago, IL, USA). Thereafter ...
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bioRxiv - Neuroscience 2022Quote: ... and 500 μg of total protein from the supernatants was precleared with Protein G Sepharose 4 Fast Flow (GE Healthcare, catalog #GE17-0618-01) for 2 h at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... Single labeled protein was isolated via ion-exchange chromatography (Mono S 5/50 GL, GE Healthcare - protein bound in buffer A (+5 mM βME) and eluted with 0-40% buffer B (buffer A + 1 M NaCl ...
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bioRxiv - Microbiology 2023Quote: ... Importin-α and Ran proteins lacking GST were prepared by cleavage with PreScission protease (20 U/mg of fusion protein; GE Healthcare, Uppsala, Sweden) at 4 °C for 12 h ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred onto polyvinylidene difluoride (PVDF) membrane (GE healthcare). The membrane was blocked with 5% dry milk in PBS containing 0.1% v/v Tween 20 (PBST ...
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bioRxiv - Biophysics 2021Quote: ... on an AKTA Pure 25L protein purification system (GE Healthcare). The column was washed with 10 column volume (CV ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein was purified on an equilibrated HisTrap (GE Healthcare) and step-eluted in wash buffer (100 mM HEPES ...
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Receptor tyrosine kinases regulate signal transduction through a liquid–liquid phase separated statebioRxiv - Cell Biology 2019Quote: ... Proteins were immobilized on Glutathione Sepharose (GE Healthcare Life Science) or StrepTactin™ Sepharose (GE Healthcare Life Science ...
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bioRxiv - Cell Biology 2019Quote: ... Protein A sepharose CL-4B was purchased from GE Healthcare. Antibodies directed toward the following antigens were also purchased ...
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bioRxiv - Cell Biology 2020Quote: ... and proteins were transferred to nitrocellulose membranes (GE Healthcare, 10600007). Antibodies used for western blots are listed in Supplementary Table 1 ...
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bioRxiv - Immunology 2021Quote: ... and protein bands were visualized with ECL Prime (GE Healthcare) or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... supernatants were purified using Protein G Sepharose beads (GE Healthcare), dialyzed against PBS ...
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bioRxiv - Genetics 2021Quote: ... proteins were detected with Amersham ECL Prime (GE Life Sciences). The following antibodies were used ...
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bioRxiv - Genetics 2021Quote: ... Proteins were transferred to Hybond-P PVDF membranes (GE Healthcare) in 1 × NuPAGE Transfer Buffer (Invitrogen by ThermoFisher ...
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bioRxiv - Biophysics 2021Quote: ... Protein was purified via FPLC (AKTA prime system, GE Healthcare) using HisTrap HP columns and eluted in 25 mM Tris ...
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bioRxiv - Biophysics 2020Quote: ... The digested protein was subjected to Superdex 200 (GE Healthcare) size exclusion chromatography in PBS to yield to pure anchor unit.
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred on a PVDF membrane (GE Healthcare, 10600021) via wet electroblotting at 100 V in transfer buffer (20% methanol ...
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bioRxiv - Neuroscience 2021Quote: ... and proteins were electrophoretically transfected onto nitrocellulose membranes (GE Healthcare). Membranes were blocked in blocking buffer (Li-COR Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were detected with the AI-600 image (GE Healthcare) using SuperSignal West Dura Extended Duration Substrate (Thermo) ...
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bioRxiv - Immunology 2022Quote: ... a 1:1 solution of Protein A Sepharose (GE Healthcare) and Protein G Sepharose (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... Separated proteins were transferred onto nitrocellulose membranes (GE Healthcare Amersham) which were subsequently blocked in 5% milk PBS for 1 h at room temperature before being probed with relevant antibodies O/N at 4°C in 5% milk PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... on Protein G Sepharose 4 Fast Flow beads (GE healthcare). The samples were incubated at 4°C overnight and then washed with progressively more stringent conditions ...
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bioRxiv - Molecular Biology 2020Quote: ... then with protein A-Sepharose CL-4B beads (GE Healthcare) for another 45 min ...
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bioRxiv - Cell Biology 2022Quote: ... and protein levels were quantified using ImageQuant software (GE Healthcare). Results were normalized to GAPDH and then to Sham values.
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bioRxiv - Cell Biology 2022Quote: ... The proteins were concentrated using a Vivaspin concentrator (GE Healthcare) and passed through a 0.2 µm Ultrafree-MC centrifugal filter (EMD Millipore ...
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bioRxiv - Genomics 2020Quote: ... Resolved proteins were transferred to a nitrocellulose membrane (GE Healthcare) and immunoblotted with the following antibodies at a 1/1,000 dilution ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was bound to a SP column (GE Healthcare) and eluted with a NaCl gradient ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were purified on an ÄKTApurifier 10 system (GE Healthcare) using 1 ml HisTrap HP columns (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... To immunoprecipitate sorLA proteins 70 μL GammaBind Beads (GE Healthcare) coated with sol-sorLA antibody was used for each sample ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were detected by adding ECL substrate (GE Healthcare, RPN2106), then exposing and visualizing using a digital developing machine (ChemiDocTM Touch Imaging System).
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bioRxiv - Genetics 2019Quote: ... and 20 μl of protein G sepharose beads (GE Healthcare) blocked with Escherichia coli DNA and bovine serum albumin ...
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bioRxiv - Microbiology 2019Quote: ... Protein bands were detected by enhanced chemiluminescence (GE Healthcare, UK) or 3,3’,5,5’-tetramethylbenzidine (TMB ...
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bioRxiv - Biochemistry 2021Quote: ... the protein was captured on glutathione sepharose beads (GE Healthcare) and eluted with 10 mM reduced glutathione ...
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bioRxiv - Microbiology 2021Quote: ... 240 mg of protein A-Sepharose CL-4B (GE Healthcare), and 1.9 ml of cell lysate ...
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bioRxiv - Microbiology 2021Quote: ... protein blots were imaged using the ImageQuant LAS4000 (GE Healthcare). The band intensity was quantified using Fiji software.
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bioRxiv - Cell Biology 2021Quote: ... Proteins were detected by enhanced chemiluminescence detection (ECL, GE Healthcare). Densitometric analyses of immunoblots were performed using ImageJ 1.50i (National Institute of Health).
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bioRxiv - Biophysics 2021Quote: ... Proteins were purified by affinity chromatography (HisTrap FF, GE Healthcare). The purest fractions were further purified through a Superdex 200 16/600 column (GE Healthcare).
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bioRxiv - Biophysics 2021Quote: ... Proteins were purified by affinity chromatography (HisTrap FF, GE Healthcare). The purest fractions were desalted through a PD10 column (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... Isolated proteins were desalted using PD-10 columns (GE Healthcare) and exchanged into BRB80 buffer (80 mM piperazine-1,4-bis(2-ethanesulfonic acid) ...
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bioRxiv - Plant Biology 2021Quote: ... protein lysates were incubated with glutathione Sepharose beads (GE Healthcare) in E buffer for 2 h ...
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bioRxiv - Systems Biology 2020Quote: ... biotinylated proteins were probed using HRP-conjugated Streptavidin (GE Healthcare Life Science ...