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Citations for GE Life Sciences :
151 - 200 of 4615 citations for Borrelia burgdorferi C t Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... All fusion proteins were purified by using a protein A affinity column (GE Healthcare) and analyzed on SDS-PAGE in the reducing condition.
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bioRxiv - Immunology 2023Quote: ... Fc-tagged ACE2 protein was then purified with a Protein A column (GE Healthcare) followed by size exclusion chromatography.
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysate was incubated with Protein G SepharoseTM 4 Fast Flow beads (GE Healthcare) for pre-clearance for 30 min at 4°C ...
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bioRxiv - Genomics 2020Quote: ... diluted in 2.5% skimmed milk in TBS-T or anti-mouse IgG conjugated to HRP (GE Healthcare) diluted in TBS-T ...
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bioRxiv - Immunology 2021Quote: ... moDC were gently washed twice with T cell expansion medium (RPMI supplemented with HyClone FBS [GE Healthcare, Chicago ...
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bioRxiv - Neuroscience 2022Quote: All subjects underwent multi-modal MRI on a 3.0 T MR scanner (MR750, GE Healthcare, Milwaukee, USA) equipped with an 8-channel brain phased array coil ...
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bioRxiv - Molecular Biology 2019Quote: Primary CD4+ T cells were isolated from buffy coats from healthy donors by Ficoll gradient (GE Healthcare) followed by negative selection with RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCells Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunoblots were washed with TBS-T and incubated with HRP-conjugated secondary antibodies (GE Healthcare, 1:5,000) for one hour in room temperature before development in films ...
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bioRxiv - Neuroscience 2023Quote: ... t-SNARE or syntaxin-1A liposomes (1.25 µmol lipid) were loaded onto a midi column (GE Healthcare) equilibrated with desalting buffer 2 containing trehalose (1 mM HEPES-KOH ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein G beads (GE Healthcare) were washed thrice with 10 bed volumes BBN buffer ...
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bioRxiv - Cell Biology 2019Quote: ... protein G-sepharose (GE Healthcare) (30 µl resin per 1 ml extract ...
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bioRxiv - Cell Biology 2020Quote: ... protein A Sepharose (GE healthcare) bead pre-equilibrated with IP dilution buffer was added and nutated further for 2 hr at 4°C for immunoprecipitation ...
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bioRxiv - Cell Biology 2020Quote: ... protein A-sepharose (GE Healthcare) beads were added to the cell lysate-antibody mixture and incubated at 4°C for 2 hours ...
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bioRxiv - Microbiology 2021Quote: ... Protein G Sepharose (GE Healthcare) was used for purification of CSP antibody.
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bioRxiv - Immunology 2021Quote: ... or protein A (GE Healthcare) (for IgG ...
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bioRxiv - Neuroscience 2023Quote: ... Protein G Sepharose (GE Healthcare) was added and incubated at 4C for 1 hr ...
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bioRxiv - Biochemistry 2020Quote: ... The solution was dialyzed against Buffer C overnight at 4 °C and applied on a Resource Q column (GE Healthcare) that was equilibrated with Buffer C ...
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bioRxiv - Cell Biology 2020Quote: ... antigen affinity columns were prepared by conjugating purified antigens (hexa-histidine-tagged PICH C-terminus fragment or GST-tagged TopoIIα C-terminus fragment) to the NHS-Sepharose resin following manufacture’s protocol (GE healthcare). The rabbit antisera were subjected to affinity purification using antigen affinity columns ...
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bioRxiv - Cell Biology 2019Quote: ... antigen affinity columns were prepared by conjugating purified antigens (hexa-histidine-tagged PICH C-terminus fragment or GST-tagged TopoIIα C-terminus fragment) to the NHS-Sepharose resin following manufacture’s protocol (GE healthcare). The rabbit antisera were subjected to affinity purification using antigen affinity columns ...
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bioRxiv - Bioengineering 2024Quote: Single-cycle kinetics analysis of CD4-Ig binding to the 16055-ConM-v8.1 SOSIP with C-terminal Spy-tag-2 (ST2) was performed at 25°C on a Biacore T100 (GE Healthcare). 1X HBS-EP containing 10 mM HEPES ...
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bioRxiv - Biophysics 2020Quote: ... onto a Hybond-C extra membranes (GE Healthcare). Membrane strips were blocked with 3% fatty acid-free BSA (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... at 25°C using Biacore T200 (GE Healthcare). The 6×His-tagged AVR-PikD (ligand ...
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bioRxiv - Immunology 2022Quote: ... at 20 °C in HBS-EP+ (GE Healthcare) running buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was Hybond-C nitrocellulose (GE Healthcare), and transfer was performed in 25 mM Tris ...
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bioRxiv - Genomics 2023Quote: ... transferred onto Hybond C nitrocellulose membrane (GE Healthcare), probed with primary antibodies anti-RRM diluted 1:300 ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were purified with the glutathione-Sepharose 4B protein chromatography purification kit (GE Healthcare). Total protein was induced by isopropyl-β-D-thiogalactopyranoside ...
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bioRxiv - Cell Biology 2020Quote: Protein-Protein interaction measurements were carried out on a BIACORE X100 (GE Healthcare Life Sciences). Sensorgrams were obtained as single cycle kinetics runs ...
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bioRxiv - Molecular Biology 2019Quote: ... Approximately 1mg protein was pre-cleared using 10µL of 50% protein G Beads (GE healthcare) by rotating for 15 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... CHO supernatants were flowed through columns packed with Protein L or Protein A (GE Healthcare) in 50mM Tris ...
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bioRxiv - Cell Biology 2019Quote: ... Antibody-conjugated proteins were collected by incubating with Protein A Sepharose beads (17528001, GE Healthcare). Beads were then washed 4-5 times with the lysis buffer described above ...
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bioRxiv - Molecular Biology 2021Quote: ... Mss116 protein was further purified by fast protein liquid chromatography (FPLC) Superdex 200 (GE Healthcare), the protein purity was checked by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE ...
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bioRxiv - Genomics 2021Quote: ... two sets of 20 µL of Protein A or Protein G Sepharose beads (GE Healthcare) were washed three times with nuclear lysis buffer for immunoprecipitation with antibody raised in guinea pig or in rabbit respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... Sample protein masses were assessed with Amersham high molecular weight protein marker mix (GE Healthcare) with proteins porcine thyroglobulin (Rs=17.0nm) ...
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bioRxiv - Microbiology 2020Quote: ... GST fusion protein and His fusion protein were purified with glutathione-Sepharose 4B (GE Healthcare) and Ni-NTA Agarose (QIAGEN ...
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bioRxiv - Cell Biology 2023Quote: ... The complex was pulled down using Protein A or Protein G-sepharose beads (GE Healthcare) pre-equilibrated in lysis buffer for 2 h ...
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bioRxiv - Bioengineering 2023Quote: For all protein variants a protein A chromatography (MabSelect SuRe resin, GE Healthcare, Uppsala, Sweden) was used as capture step ...
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bioRxiv - Immunology 2023Quote: ... the filtered supernatant ran through a protein A and protein G affinity column (GE Healthcare) and eluted with 0.2 M citric acid at pH 3.0 and neutralized in 2 M Tris-base ...
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bioRxiv - Biochemistry 2019Quote: ... Glutathione was removed and CMG2 exchanged into HBS-T with 50% glycerol using Sephadex G50 (GE Life Sciences) on an Äkta Start chromatography system.
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bioRxiv - Immunology 2022Quote: ... Total CD4+ T cells were collected from buffy coats by first performing a Ficoll-Paque Plus (GE Healthcare) density gradient centrifugation to isolate peripheral blood mononuclear cells (PBMC ...
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bioRxiv - Genomics 2022Quote: ... with an anti-rabbit IgG (goat polyclonal, 1:10,000 in 3% BSA/TBS-T; GE Healthcare, #RPN4301, RRID:AB_2650489) secondary antibody was used as a loading control ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... Membranes were washed with PBS-T for 3 × 10 min and were developed by ECL Prime (GE Healthcare) and imaged on a Biorad ChemiDoc MP Imaging System.
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bioRxiv - Neuroscience 2023Quote: ... MRI images were acquired with the aid of a 3-T scanner (Discovery 750; GE Healthcare, Milwaukee, WI) and an 8-channel head coil ...
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bioRxiv - Microbiology 2023Quote: ... The relevant oligonucleotide of SV40 large T antigen NLS (PPKKKRKVED) was ligated into the pGEX2T vector (GE Healthcare) carrying the GFP gene at the C-terminus of the multi-cloning site to produce GST-SV40NLS-GFP protein (referred to as SV40 NLS) ...
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bioRxiv - Microbiology 2019Quote: ... the protein was purified using HisTrap HP histidine-tagged protein columns (GE healthcare, Chicago, IL, USA) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2020Quote: ... followed by incubation with protein A or protein G Sepharose beads (GE Healthcare, Milwaukee, WI, USA) for 3 h with gentle rocking in the cold room ...
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bioRxiv - Immunology 2020Quote: ... The IgG was then purified using protein G affinity chromatography (HiTrap Protein G HP, GE Healthcare) and size-exclusion chromatography (Superdex 200 ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted proteins were exchanged into protein storage buffer on an NAP-5 column (GE Healthcare, 17085301), concentrated with a 50 kDa MWCO Amicon Ultra-0.5 device (Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... Large scale protein purification was done using ÄKTA prime Fast Liquid Protein Chromatography System (GE Healthcare). DE droplets were analyzed on a BD LSR Fortessa SORP (Special Order Research Product ...
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bioRxiv - Immunology 2021Quote: ... and the protein within the supernatant was purified with a Protein A-Sepharose column (GE Healthcare) according to the manufacturer’s instruction for primary purification ...
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bioRxiv - Biochemistry 2022Quote: Antibodies against CFTR were pre-incubated with protein-A or protein-G Sepharose beads (GE Healthcare) for 15 minutes at 4°C before adding protease-treated or non-treated lysates ...