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Citations for GE Life Sciences :
501 - 550 of 4459 citations for Borrelia Miyamotoi GlpQ Protein His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The GST tag of N-terminal GST-6xHis-RhoGAP domain was removed by addition of PreScission protease (GE healthcare Lifesciences) directly to the glutathione beads followed by incubation overnight at 4°C to obtain His-tagged RhoGAP ...
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bioRxiv - Immunology 2020Quote: ... to remove the GST tag and further purified by gel filtration (SuperoseTM 6 prep grade XK 16/70; GE Healthcare). To prepare for crystallization trials ...
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bioRxiv - Microbiology 2024Quote: ... The eluted Cas1 without tag was concentrated and further purified by gel filtration chromatography (Superdex200 10/300 GL, GE Healthcare) in buffer B containing 10 mM Tris pH 8.0 ...
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bioRxiv - Bioengineering 2024Quote: Single-cycle kinetics analysis of CD4-Ig binding to the 16055-ConM-v8.1 SOSIP with C-terminal Spy-tag-2 (ST2) was performed at 25°C on a Biacore T100 (GE Healthcare). 1X HBS-EP containing 10 mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: ... 1,000 μg of proteins were prepared and pre-cleared with 20 μl of protein A-Sepharose and protein G-Sepharose beads mixture (GE Healthcare). 2 μl of (HA ...
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bioRxiv - Biophysics 2021Quote: ... The purification was done by purifying these two supernatants with 1 M NaCl containing Tris buffer using Hi-Trap Q-FF anion exchange column of ÄKTA Pure (GE healthcare). To maintain the oxidation activity of DsbA 0.3% H2O2 was added to the purified protein for overnight ...
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bioRxiv - Cell Biology 2020Quote: ... The clear supernatant layer was collected and run over five serially connected Hi-Trap desalting columns (GE healthcare, 17-1408-01) in buffer A supplemented with 2 mM DTT and 14% glycerol ...
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bioRxiv - Microbiology 2021Quote: ... Further purification was carried out by size exclusion chromatography using a Superdex™ 200 Hi Load 16/60 column (GE Healthcare). Proteins were eluted with SEC buffer (25 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysed cells were centrifuged 20,000 xg for 30 minutes at 4C and soluble lysate was filtered prior to IMAC purification using 2×5mL His-Trap columns (GE Healthcare) affixed to an Akta Pure ...
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bioRxiv - Molecular Biology 2020Quote: ... the buffer was supplemented with 30 mM imidazole and an additional 100 mM NaCl and passed through a 1 ml Hi-Trap Chelating column (GE Healthcare) to capture the N-terminal fragment that was cleaved by the TEV protease ...
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bioRxiv - Biophysics 2019Quote: ... Sample was further purified from free labelled peptide and Sortase by size-exclusion chromatography using a Hi/Load 16/600 Superdex 75 column (GE Healthcare) previously equilibrated with ZapA storage buffer ...
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Ouabain enhances cell-cell adhesion mediated by β1-subunits of the Na+,K+-ATPase in CHO fibroblasts.bioRxiv - Cell Biology 2019Quote: Total extract of CHO cells expressing the β1His6 construct were immobilized with nickel-nitrilotriacetic acid beads (Ni+-NTA, His Trap FF column; GE Healthcare) equilibrated with 10 ml of RIPA buffer containing protease inhibitors ...
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bioRxiv - Microbiology 2019Quote: ... After cell lysis by sonication cell debris was removed by centrifugation and the lysate was passed through a His-Trap column (GE Healthcare). Proteins were eluted with a gradient of buffer containing 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... except that purification by size exclusion was omitted and the solubilized inclusion bodies were pre-cleaned by passage over a Hi-Trap Q-Sepharose column (GE healthcare) before loading onto a Hi-Trap SP-Sepharose column (GE healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were concentrated using Amicon Ultra-15 concentrators and further separated by HiTrap Heparin HP column and Hi-load 16/600 superdex 200 column (GE Healthcare). Fractions from the column were pooled and subjected for SEC-MALS analysis to determine their oligomeric status ...
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bioRxiv - Microbiology 2021Quote: ... The liposomes were then purified by gel filtration using a Hi Prep 16/60 Sephacryl S-300 HR column (GE Healthcare) in 150 mM NaCl ...
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... the bacterially expressed 6×His tagged ARL3 and its variants were purified with Ni SepharoseTM 6 Fast Flow beads (GE Healthcare) and cleaved with thrombin (Solarbio ...
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bioRxiv - Plant Biology 2020Quote: ... Anti-NPR1 antibodies were purified from antisera by affinity chromatography using a tNPR1-His-immobilized column (Hitrap NHS-activated HP; GE Healthcare). To prepare for the tNPR1-His-immobilized column ...
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bioRxiv - Microbiology 2020Quote: ... The elution fractions were concentrated and (His)6-Rv3341 was further purified by gel exclusion chromatography using Superdex 200 Increase 10/300 GL column (GE Healthcare). The purified fractions were pooled ...
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bioRxiv - Microbiology 2020Quote: ... Solubilized IBs were purified by immobilized metal affinity chromatography (IMAC) by loading onto a prepacked 1 ml His-Trap HP column (GE Healthcare) equilibrated with lysis buffer containing 8M urea at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare) ...
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bioRxiv - Biophysics 2020Quote: ... Immunoblots with anti-HA, anti-His or anti-SecB antibodies were visualized by Lumino image analyzer LAS-4000 mini (Cytiva, Tokyo, Japan (GE Healthcare)) using ECL Prime Western Blotting Detection Reagents (Cytiva ...
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bioRxiv - Developmental Biology 2022Quote: ... The precipitants were dialyzed against a 400 times volume of PBS (-) plus Triton X-100 and then applied to a Nickel (Ni)-Sepharose Histidine-affinity column (His GraviTrap; GE Healthcare). Bound proteins were eluted with PBS (- ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Insoluble material was pelleted at 50,000 xg for 30 min at 4°C and the supernatant loaded directly onto a 5 ml His Trap HP (GE Healthcare) at 2 ml.min-1 and washed with 50 ml Buffer A at 4 m.min-1 at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... His-tagged Ri-boswitch-VLPs were purified using a Ni beads gravity column loaded with high-performance Ni beads (GE Healthcare). Briefly ...
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bioRxiv - Biochemistry 2022Quote: (His)6-GST-CAPN7(MIT)2 was bound to GST-sepharose beads (10 mL,GE Healthcare Life Sciences, USA, 6 h), washed with 1 L wash buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Immobilized metal ion affinity chromatography of the His-tagged Pdr5 was performed with a Zn2+ loaded 1 ml HiTrap Chelating column (GE Healthcare) and elution was carried out with a step gradient using low and high histidine buffers (50 mM Tris-HCl pH 7.8 ...
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bioRxiv - Molecular Biology 2019Quote: ... (amino acids 2-241 fused to 6 x his) were expressed in BL21 cells and purified on glutathione-linked sepharose beads (GE healthcare) or Ni-NTA-Agarose (GE healthcare ...
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bioRxiv - Microbiology 2021Quote: Purified His-tagged Pentamer was immobilized to a single flow cell of a NiNTA sensor in a Biacore X100 (GE Healthcare) to a level of ∼800 response units (RUs ...
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bioRxiv - Microbiology 2021Quote: ... HisTRAP eluted peak fractions were pooled and applied to a Hi-Load 16/600 Superdex-200 (preparation grade) size-exclusion column (GE Healthcare) equilibrated with the gel filtration buffer (50 mM Tris-HCl ...
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bioRxiv - Immunology 2021Quote: ... The peak fractions were collected and purified by size exclusion chromatography using Hi-Load 16/60 Superdex-200 (prep-grade) gel filtration column (GE Healthcare) in a buffer containing 25 mM Tris·HCl (pH 8.0 ...
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bioRxiv - Immunology 2021Quote: ... Peak protein fractions were pooled and loaded onto the size-exclusion chromatography column using Hi-Load 16/60 Superdex-200 (prep-grade) gel filtration column (GE Healthcare) in a buffer containing 25 mM Tris·HCl (pH 8.0 ...
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bioRxiv - Molecular Biology 2022Quote: 1.5 mg of pure PfP2 incubated at 37°C for 3h and was subsequently loaded onto a Hi Load Superdex 75 16/600 gel filtration column (GE Healthcare) pre-equilibrated with 20mM Tris-HCl (pH 7.4 ...
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bioRxiv - Biophysics 2019Quote: ... Cell lysate was centrifuged at 35,000 g for 60 minutes to remove insoluble cell debris and the soluble lysate was loaded onto a 5 mL His Trap HP Ni column (GE Healthcare) at a rate of 1.5 mL/minute ...
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bioRxiv - Immunology 2020Quote: ... Purification of His-HMGB1 from 293-freestyle cell lysate was carried out using Ni SepharoseTM 6 Fast Flow gel (GE Healthcare). After purification ...
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bioRxiv - Biochemistry 2020Quote: ... The liberated affinity-tag and the His-tagged Senp1 protease were removed by a second round of affinity purification with HisTrap FF columns (GE Healthcare). All proteins were purified with a final size exclusion chromatography (HiLoad 16/600 Superdex 75 or 200 pg ...
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bioRxiv - Microbiology 2020Quote: ... Lysed cells were ultracentrifuged and the cleared supernatant loaded onto a Ni Sepharose 6 Fast Flow His Trap column (GE Healthcare) equilibrated with Buffer A ...
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bioRxiv - Biochemistry 2019Quote: ... A 50 – 500 mM KCl gradient was used to elute IF2 from a 5 ml Hi-Trap SP HP column (17115201, GE Healthcare). Fractions were analysed by 8% acrylamide SDS-PAGE and those containing IF2 were pooled together ...
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bioRxiv - Molecular Biology 2019Quote: ... Solubilized IBs were purified by immobilized metal affinity chromatography (IMAC) by loading onto a prepacked 1 ml His-Trap HP column (GE Healthcare) equilibrated with lysis buffer containing 8M urea at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare) ...
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bioRxiv - Biophysics 2020Quote: ... Bacterial biomass was harvested by centrifugation at 5,400g and His-tagged nanobodies were purified using HisTrap™ column chromatography (GE Healthcare) followed by a buffer exchange step to PBS using a HiTrap™ desalting column (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: ... to prevent activation of the alternative complement pathway and complement consumption) and anti-FH mAb (35H9) on Hi-Trap HP-NHS columns (GE Healthcare). FH protein was purified from EDTA-plasma using an anti-FH mAb (35H9 ...
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bioRxiv - Biochemistry 2021Quote: ... The flow-through containing nsp12 was further purified by ion exchange chromatography using Hi TRAP Q HP and SP HP pre-packed columns (GE Healthcare) equilibrated with buffer D ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tagged sample was purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose Excel resin (GE Healthcare Life Sciences). The IMAC eluted sample was dialyzed and treated with His-tagged Human Rhinovirus 3C protease as described previously32 ...
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bioRxiv - Microbiology 2022Quote: ... Size exclusion chromatography was performed in 20 °C ÄKTA purifier system (Amersham) and a prepacked Hi-Prep 16/60 Sephacryl S-200 high-resolution column (GE Healthcare). Flow rate was 0.5 ml/min ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tagged sample was purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose Excel resin (GE Healthcare Life Sciences). The IMAC eluted sample was dialyzed and treated with His-tagged Human Rhinovirus 3C protease ...
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bioRxiv - Microbiology 2023Quote: ... Lysate was cleared by centrifugation and the supernatant loaded into an anion exchange column Hi-Trap Sepharose Q-HP (GE Healthcare). KtrA and KtrC were eluted with a 50-1000 mM KCl gradient ...
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bioRxiv - Plant Biology 2022Quote: ... radioactive signals from His-PIN1L were detected on the dried gel by the Typhoon imaging system (GE Healthcare TYPHOON FLA 9500).
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bioRxiv - Systems Biology 2023Quote: ... The lysates were clarified by centrifugation for 1h at 4ºC at 12000 rpm and loaded on a His GraviTrap™ column (GE Healthcare). After washing with 10 mL of washing buffer (40 mM sodium phosphate ...
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bioRxiv - Molecular Biology 2023Quote: ... The fusion protein was eluted using binding buffer with 250 mM imidazole and then further purified using size-exclusion chromatography on a Hi load 16/60 Sephacryl S- 100 column (GE Healthcare) in the binding buffer with pH adjusted to 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... To remove any residual impurities final step of size-exclusion chromatography on a Hi load 16/60 Sephacryl S-100 column (GE Healthcare) in the binding buffer with pH adjusted to 7.4 was employed ...
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bioRxiv - Immunology 2023Quote: Recombinant 50 kDa and 39 kDa CHIT1 proteins were expressed as C-terminally 8xHis-tagged constructs in HEK293-6E cells and purified from the cell culture supernatant by His-Trap (GE Healthcare) affinity as described in [42] ...