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Citations for GE Life Sciences :
1 - 50 of 76 citations for Arsenazo I Trisodium Salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... The triarylmethyl EPR oxygen tracer OX063 (methyl-tris[8-carboxy-2,2,6,6-tetrakis[2-hydroxyethyl]benzo[1,2-d:4,5-d0]-bis[1,3]dithiol-4-yl]-trisodium salt) was obtained from GE Healthcare.
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bioRxiv - Biophysics 2022Quote: The low-salt and high-salt fractions were further purified by using gel-filtration column Superose 6 Increase 10/300 GL (GE Healthcare). The low-salt fraction was injected into the column in SEC150 buffer (10 mM Tris 7.5 ...
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bioRxiv - Bioengineering 2020Quote: ... disposable PD10 desalting salt columns were purchased from GE Healthcare Life Sciences and Amicon® Ultra centrifugal filters from Merck Millipore.
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bioRxiv - Neuroscience 2020Quote: ... in 1X Hank’s Balanced Salt Solution (SH30588.02, GE Healthcare Life Sciences, United States). The dura was removed and the spinal cord was bisected lengthwise ...
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bioRxiv - Biochemistry 2024Quote: ... the GE Healthcare Lunar i (GE Healthcare Lunar with enCORE software version 16.2).
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bioRxiv - Neuroscience 2022Quote: ... in Hanks’ Balanced Salt Solution (HBSS) medium without calcium and magnesium (Hyclone, GE Healthcare) supplemented with 20 mg/mL DNase (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... transthoracic echocardiography (ViVid i; GE Healthcare, WI, USA) was performed with an 11.5-MHz transducer ...
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bioRxiv - Plant Biology 2020Quote: ... using a Minifold I slot blot system (GE Healthcare). Probe labeling and hybridization were as described for RNA gel blot analysis ...
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bioRxiv - Biochemistry 2021Quote: ... The phosphorylation mixture was then dialyzed against low salt buffer and loaded to a MonoS (GE HEalthcare) column ...
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bioRxiv - Zoology 2020Quote: ... The amplified products purified enzymatically with exonuclease I (GE Healthcare) and Sanger sequenced by ACTGene Inc. ...
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bioRxiv - Physiology 2022Quote: ... and with an iodine-containing non-ionic radiocontrast agent iodixanol (12 mg I/kg bodyweight, Visipaque 320 mg I/ml; GE Healthcare, Chicago, Illinois, United States). The contrast agent was injected in the scruff area ...
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bioRxiv - Neuroscience 2024Quote: ... diluted twice to lower salt concentration to 50 mM and bound to a MonoS Sepharose column (GE Healthcare). Finally ...
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bioRxiv - Biochemistry 2022Quote: ... Salt concentration is adjusted and protein is loaded on a 10 ml HiTrap Heparin HP column (GE Healthcare) equilibrated in 20 mM HEPES- KOH pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... after which the reaction mixture was passed through the salt exchange column (HiPrep 16/10 Desalting, GE Healthcare) with FPLC UV filter set at 254nm and then concentrated using 10kDa concentrator ...
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bioRxiv - Physiology 2020Quote: A GE Lunar DXA (i-DXA, GE Healthcare Switzerland, Glattbrugg, Switzerland) with a narrow-angle fan beam (Collimator Model 42129 ...
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bioRxiv - Biochemistry 2024Quote: ... I-PpoI was loaded onto a HisTrap HP column (GE Healthcare), which was pre-equilibrated with a buffer that contained 50 mM Tris (pH 7.5) ...
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bioRxiv - Cell Biology 2020Quote: ... Umbilical cords were flushed with Hank’s balanced salt solution with magnesium and calcium (HyClone™, GE Healthcare, Solingen, Germany) and PBS with magnesium chloride and calcium chloride (Merck KGaA ...
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bioRxiv - Microbiology 2020Quote: ... the redundant salt in mixture was removed in a G25 desalting column (GE Healthcare Life Sciences, Pittsburgh, PA, USA) equilibrated with 50 mM HEPES solution (pH 7.3).
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bioRxiv - Cell Biology 2024Quote: ... Monomeric forms were purified from potential oligomeric species and salt using a Superdex 75 10⁄300 GL column (GE Healthcare) at a flowrate of 0.7 mL/min ...
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bioRxiv - Microbiology 2021Quote: ... The protein sample was exchanged into low salt buffer (20 mM Tris pH 8, 20 mM NaCl, 0.03% DDM) using a PD-10 column (GE Healthcare) and then injected onto a MonoQ column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... and eluted by gradient introduction of high-salt buffer (50mM Tris pH7.0, 1M NaCl, 2mM MgCl2, 1mM ATP, 2mM beta-mercaptoethanol) on an ÅKTA system (GE Healthcare). Protein-containing pooled fractions from IEX were then loaded onto a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... hippocampi were subjected to enzymatic digestion in Hanks′ Balanced Salt Solution (HBSS) medium without calcium and magnesium (Hyclone, GE Healthcare) supplemented with Trypsin (1x ...
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bioRxiv - Biophysics 2022Quote: ... hSERINC3 was eluted in low salt buffer contain 2.5 mM desthiobiotin and then concentrated using a centrifugal filter (Vivaspin, 50 kDa MWCO (GE Healthcare)) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Monomeric forms were purified from potential oligomeric species and salt using a Superdex 75 10/300 GL column (GE Healthcare) at a flowrate of 0.5 mL/min ...
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bioRxiv - Biophysics 2023Quote: ... Monomeric forms were purified from potential oligomeric species and salt using a Superdex 75 10/300 GL column (GE Healthcare) at a flow rate of 0.5 mL min−1 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was further purified by salt gradient ion exchange chromatography using a Mono Q 5/50 GL column (GE healthcare) and Gradient buffers (20 mM HEPES-KOH pH 7.6 ...
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... GST-RIG-I protein variants were affinity purified by GSTrap column (GE Healthcare). After sample loading ...
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bioRxiv - Biophysics 2022Quote: ... and then purified from excess salt and potential oligomeric species using gel filtration on a size exclusion column (Superdex 75 10/300 GL, GE Healthcare) at a flow rate of 0.5 mL/min and eluted in 20 mM sodium phosphate 0.2 mM EDTA buffer (at pH 8.0) ...
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bioRxiv - Developmental Biology 2021Quote: ... Eluted protein was diluted to a final salt concentration of 200 mM NaCl and loaded onto a 5 mL HiTrap SP ion-exchange column (GE Healthcare).The protein was eluted in the same buffer with a salt gradient (200mM-1 M NaCl ...
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bioRxiv - Biophysics 2022Quote: ... and purified from any other oligomeric species and salts using a Superdex 75 10/300 GL column (GE Healthcare, Chicago, IL) at a flow rate of 0.5 ml/min in 20 mM sodium phosphate buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The eNOR containing fraction was eluted at around 250 mM salt and the eluted fraction was then loaded on a Chelating Sepharose (GE Healthcare) column ...
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bioRxiv - Biochemistry 2021Quote: ... bound protein was eluted using a gradient increasing the salt concentration to 500 mM KCl over 100 mL while collecting fractions (AEKTA prime FPLC system, GE Healthcare). Fractions were analyzed by SDS-PAGE and those containing pure full-length TwinStrep-UPF1 were collected ...
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bioRxiv - Genomics 2020Quote: ... Proteins were eluted with 3mL elution buffer (PBS, 250mM Imidazole) and excess salt was removed using the desalting column according to the manufacture’s protocol (GE Healthcare, 17085101). Subsequently ...
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bioRxiv - Bioengineering 2023Quote: ... where 18 mL of blood was mixed with Hanks’ Balanced Salt Solution (HBSS, 14175095, GibcoTM) before being poured onto Ficoll-Paque Plus (GE17-1440-03, GE Healthcare). The layers were separated through centrifugation ...
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bioRxiv - Biophysics 2023Quote: ... The sample was then diluted with dilution buffer (50 mM HEPES (pH 7.2) and 5% glycerol) to reduce the salt concentration and loaded onto a Mono S 10/100 GL column (GE Healthcare). The column was washed with buffer C (50 mM HEPES (pH 7.2) ...
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bioRxiv - Biochemistry 2022Quote: ... both MSL8N and MSL9N were placed in a high salt storage buffer (1 M NaCl, 200 mM Sodium Phosphate) using gravity PD-10 desalting columns (GE Healthcare). The protein was labeled with Alexa Fluor 647 NHS (Invitrogen ...
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bioRxiv - Biophysics 2022Quote: ... was diluted to a final salt concentration of 200 mM NaCl before loading onto a 1 mL heparin column (GE Healthcare) equilibrated in low-salt buffer (50 mM Tris pH 7.4 ...
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bioRxiv - Biophysics 2023Quote: ... diluted with the same buffer devoid of NaCl to reach a final salt concentration of 40 mM and loaded on a HiSure Q 5 mL column (GE Healthcare). After washing with a 25 mM Na-HEPES ...
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bioRxiv - Neuroscience 2020Quote: ... 20–40 g) and placed on ice in Petri dishes containing 1X Hank’s Balanced Salt Solution (HBSS, SH30588.02, GE Healthcare Life Sciences, US). Ganglia were minced using surgical micro scissors and incubated in a papain solution (32 μL papain (27.3 U/mg ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted protein was then diluted to a final salt concentration of low salt buffer conditions (100 mM KCl, 50 mM HEPES, 1 mM MgCl2) and bound to HiTrap SP FF (GE Healthcare, USA) anion exchange column ...
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bioRxiv - Microbiology 2023Quote: ... purified using Exo (exonuclease I) –SAP (shrimp alkaline phosphatase) (GE Healthcare, Little Chalfont, UK) method by following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2021Quote: ... Whole blood was diluted 1:1 with balanced salt solution at RT and layered onto Ficoll Paque PLUS (GE Healthcare, #GE17-1440-02) followed by centrifugation at 400 g for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was loaded on a His-Trap FF column (GE Healthcare, Chicago, I L) equilibrated with lysis buffer and washed with 3 CV lysis buffer containing 20 mM imidazole and washed again with 3 CV lysis buffer containing 50 mM imidazole ...
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bioRxiv - Cell Biology 2021Quote: Cardiac function was assessed using cardiac echography under general anesthesia (ViVid i; GE Healthcare, WI, USA) with an 11.5-MHz transducer every week until 4 weeks after implantation ...
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bioRxiv - Immunology 2021Quote: ... for RIG-I and TRIF analysis on an ImageQuant LAS 4000 mini biomolecular imager (GE Healthcare). Membranes were washed with TBS-T ...
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bioRxiv - Biochemistry 2023Quote: ... Dissociated pMHC I and TAPBPR were isolated by SEC (Superdex 200 Increase 3.2/300, GE Healthcare) in 1×HBS ...
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bioRxiv - Cell Biology 2023Quote: ... as a model of pre-adipocytes [30] were cultured in DMEM (Nacalai, Osaka, Japan) supplemented with 10% fetal bovine serum (HyClone; GE Healthcare, Salt Lake City, UT, USA) and a mixture of 100 units/ml penicillin and 100 μg/ml streptomycin (Nacalai ...
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bioRxiv - Cell Biology 2024Quote: ... Public Health England, London, UK) were maintained in DMEM (Nacalai, Osaka, Japan) with 10% fetal bovine serum (FBS) (HyClone; GE Healthcare, Salt Lake City, UT, USA) and a mixture of 100 units/ml penicillin and 100 μg/ml streptomycin (P/S ...
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bioRxiv - Cell Biology 2021Quote: ... 2012) with the following modifications: (I) the GUVs were desalted two times employing a PD10 column (GE Healthcare) instead of using a Sephadex-G50 gel filtration column in the second desalting step ...
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bioRxiv - Plant Biology 2020Quote: ... Fractions containing complex I were pooled and loaded onto a Superose 6 Increase 10/300 column (GE Healthcare) equilibrated with buffer containing 30 mM HEPES-NaOH ...