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Citations for GE Life Sciences :
1 - 50 of 111 citations for Aprepitant Beta Glucuronide Sodium Salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... and eluted by gradient introduction of high-salt buffer (50mM Tris pH7.0, 1M NaCl, 2mM MgCl2, 1mM ATP, 2mM beta-mercaptoethanol) on an ÅKTA system (GE Healthcare). Protein-containing pooled fractions from IEX were then loaded onto a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... both MSL8N and MSL9N were placed in a high salt storage buffer (1 M NaCl, 200 mM Sodium Phosphate) using gravity PD-10 desalting columns (GE Healthcare). The protein was labeled with Alexa Fluor 647 NHS (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... beta-Pix/ArghGEF7 (J-009616-07, Dharmacon GE Healthcare) or untargetting control were used ...
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bioRxiv - Biophysics 2022Quote: The low-salt and high-salt fractions were further purified by using gel-filtration column Superose 6 Increase 10/300 GL (GE Healthcare). The low-salt fraction was injected into the column in SEC150 buffer (10 mM Tris 7.5 ...
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bioRxiv - Bioengineering 2020Quote: ... disposable PD10 desalting salt columns were purchased from GE Healthcare Life Sciences and Amicon® Ultra centrifugal filters from Merck Millipore.
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bioRxiv - Molecular Biology 2021Quote: ... using 10mM sodium acetate pH4.0 solution (GE Healthcare). The surface of the sensor chip was blocked with 1M ethanolamine-HCl pH8.5 (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: ... 1X sodium pyruvate (HyClone, GE Healthcare, Chicago, IL), and 1X non-essential amino acids (HyClone ...
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bioRxiv - Neuroscience 2020Quote: ... in 1X Hank’s Balanced Salt Solution (SH30588.02, GE Healthcare Life Sciences, United States). The dura was removed and the spinal cord was bisected lengthwise ...
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bioRxiv - Neuroscience 2021Quote: ... 20 mg/ml sodium dodecyl sulfate (SDS, GE Healthcare), 40 nM N-ethylmaleimide (NEM ...
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bioRxiv - Neuroscience 2022Quote: ... in Hanks’ Balanced Salt Solution (HBSS) medium without calcium and magnesium (Hyclone, GE Healthcare) supplemented with 20 mg/mL DNase (Roche ...
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bioRxiv - Biophysics 2021Quote: ... Cleaved products were further purified by S-75 size exclusion chromatography (SEC buffer-50 mM sodium phosphate, 50 mM sodium chloride, 1 mM DTT, pH 8) (GE Healthcare Life Sciences). The purity of the protein was inspected using a 15% SDS PAGE (Figure S1) ...
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bioRxiv - Pathology 2021Quote: ... Excess reagents were removed by repeated diafiltration into fresh PBS buffer (140 mM sodium chloride and 12 mM sodium phosphates at pH 7.4) using VivaSpin sample concentrators (GE Healthcare, 10,000 Da MWCO), and the volume was corrected to 200 μL ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... NHR2 was then purified by S-75 size exclusion chromatography (SEC buffer-50 mM sodium phosphate, 50 mM sodium chloride, 1 mM DTT, pH 8) (GE Healthcare Life Sciences) and analyzed using SDS-PAGE ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM sodium pyruvate (HyClone, GE Healthcare Life Sciences, catalog # SH3023901), 100 µM β-mercaptoethanol ...
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bioRxiv - Cancer Biology 2020Quote: ... the membranes were incubated with 10,000-fold diluted peroxidase-linked secondary antibody (NA931V for FLAG-BRCA2 and NA934V for beta-Actin; GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Biochemistry 2021Quote: ... The phosphorylation mixture was then dialyzed against low salt buffer and loaded to a MonoS (GE HEalthcare) column ...
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bioRxiv - Genetics 2024Quote: ... without L-Glutamine and Sodium Pyruvate (GE Healthcare Life Sciences Cat. # SH30081.01); 1X GlutaMAX-I (Thermo Fisher Scientific Cat ...
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bioRxiv - Neuroscience 2023Quote: ... 1.5% Sodium dodecyl sulfate (SDS; GE Healthcare, cat. no 17-1313-01), 8% glycerol (Alfa Aesar ...
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bioRxiv - Cancer Biology 2020Quote: ... The triarylmethyl EPR oxygen tracer OX063 (methyl-tris[8-carboxy-2,2,6,6-tetrakis[2-hydroxyethyl]benzo[1,2-d:4,5-d0]-bis[1,3]dithiol-4-yl]-trisodium salt) was obtained from GE Healthcare.
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bioRxiv - Biochemistry 2022Quote: ... Salt concentration is adjusted and protein is loaded on a 10 ml HiTrap Heparin HP column (GE Healthcare) equilibrated in 20 mM HEPES- KOH pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... after which the reaction mixture was passed through the salt exchange column (HiPrep 16/10 Desalting, GE Healthcare) with FPLC UV filter set at 254nm and then concentrated using 10kDa concentrator ...
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bioRxiv - Neuroscience 2024Quote: ... diluted twice to lower salt concentration to 50 mM and bound to a MonoS Sepharose column (GE Healthcare). Finally ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 150 mM sodium chloride (HBS) and loaded onto protein G resin (GE Healthcare) equilibrated with HBS ...
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bioRxiv - Microbiology 2022Quote: ... 0.02% sodium azide by using a HiPrep 26/10 desalting column (GE Healthcare). This ensured that the proteins were maintained in their monomeric form ...
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bioRxiv - Cell Biology 2020Quote: ... Umbilical cords were flushed with Hank’s balanced salt solution with magnesium and calcium (HyClone™, GE Healthcare, Solingen, Germany) and PBS with magnesium chloride and calcium chloride (Merck KGaA ...
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bioRxiv - Microbiology 2020Quote: ... the redundant salt in mixture was removed in a G25 desalting column (GE Healthcare Life Sciences, Pittsburgh, PA, USA) equilibrated with 50 mM HEPES solution (pH 7.3).
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bioRxiv - Cell Biology 2024Quote: ... Monomeric forms were purified from potential oligomeric species and salt using a Superdex 75 10⁄300 GL column (GE Healthcare) at a flowrate of 0.7 mL/min ...
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bioRxiv - Microbiology 2021Quote: ... The protein sample was exchanged into low salt buffer (20 mM Tris pH 8, 20 mM NaCl, 0.03% DDM) using a PD-10 column (GE Healthcare) and then injected onto a MonoQ column (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... hippocampi were subjected to enzymatic digestion in Hanks′ Balanced Salt Solution (HBSS) medium without calcium and magnesium (Hyclone, GE Healthcare) supplemented with Trypsin (1x ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Monomeric forms were purified from potential oligomeric species and salt using a Superdex 75 10/300 GL column (GE Healthcare) at a flowrate of 0.5 mL/min ...
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bioRxiv - Biophysics 2022Quote: ... hSERINC3 was eluted in low salt buffer contain 2.5 mM desthiobiotin and then concentrated using a centrifugal filter (Vivaspin, 50 kDa MWCO (GE Healthcare)) ...
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bioRxiv - Biophysics 2023Quote: ... Monomeric forms were purified from potential oligomeric species and salt using a Superdex 75 10/300 GL column (GE Healthcare) at a flow rate of 0.5 mL min−1 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was further purified by salt gradient ion exchange chromatography using a Mono Q 5/50 GL column (GE healthcare) and Gradient buffers (20 mM HEPES-KOH pH 7.6 ...
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bioRxiv - Biophysics 2022Quote: ... and then purified from excess salt and potential oligomeric species using gel filtration on a size exclusion column (Superdex 75 10/300 GL, GE Healthcare) at a flow rate of 0.5 mL/min and eluted in 20 mM sodium phosphate 0.2 mM EDTA buffer (at pH 8.0) ...
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bioRxiv - Developmental Biology 2021Quote: ... Eluted protein was diluted to a final salt concentration of 200 mM NaCl and loaded onto a 5 mL HiTrap SP ion-exchange column (GE Healthcare).The protein was eluted in the same buffer with a salt gradient (200mM-1 M NaCl ...
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bioRxiv - Biophysics 2022Quote: ... and purified from any other oligomeric species and salts using a Superdex 75 10/300 GL column (GE Healthcare, Chicago, IL) at a flow rate of 0.5 ml/min in 20 mM sodium phosphate buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The eNOR containing fraction was eluted at around 250 mM salt and the eluted fraction was then loaded on a Chelating Sepharose (GE Healthcare) column ...
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bioRxiv - Biochemistry 2021Quote: ... bound protein was eluted using a gradient increasing the salt concentration to 500 mM KCl over 100 mL while collecting fractions (AEKTA prime FPLC system, GE Healthcare). Fractions were analyzed by SDS-PAGE and those containing pure full-length TwinStrep-UPF1 were collected ...
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bioRxiv - Genomics 2020Quote: ... Proteins were eluted with 3mL elution buffer (PBS, 250mM Imidazole) and excess salt was removed using the desalting column according to the manufacture’s protocol (GE Healthcare, 17085101). Subsequently ...
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bioRxiv - Biophysics 2022Quote: ... was diluted to a final salt concentration of 200 mM NaCl before loading onto a 1 mL heparin column (GE Healthcare) equilibrated in low-salt buffer (50 mM Tris pH 7.4 ...
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bioRxiv - Biophysics 2023Quote: ... diluted with the same buffer devoid of NaCl to reach a final salt concentration of 40 mM and loaded on a HiSure Q 5 mL column (GE Healthcare). After washing with a 25 mM Na-HEPES ...
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bioRxiv - Bioengineering 2023Quote: ... where 18 mL of blood was mixed with Hanks’ Balanced Salt Solution (HBSS, 14175095, GibcoTM) before being poured onto Ficoll-Paque Plus (GE17-1440-03, GE Healthcare). The layers were separated through centrifugation ...
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bioRxiv - Biophysics 2023Quote: ... The sample was then diluted with dilution buffer (50 mM HEPES (pH 7.2) and 5% glycerol) to reduce the salt concentration and loaded onto a Mono S 10/100 GL column (GE Healthcare). The column was washed with buffer C (50 mM HEPES (pH 7.2) ...
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bioRxiv - Neuroscience 2020Quote: ... 20–40 g) and placed on ice in Petri dishes containing 1X Hank’s Balanced Salt Solution (HBSS, SH30588.02, GE Healthcare Life Sciences, US). Ganglia were minced using surgical micro scissors and incubated in a papain solution (32 μL papain (27.3 U/mg ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted protein was then diluted to a final salt concentration of low salt buffer conditions (100 mM KCl, 50 mM HEPES, 1 mM MgCl2) and bound to HiTrap SP FF (GE Healthcare, USA) anion exchange column ...
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bioRxiv - Cell Biology 2021Quote: ... Whole blood was diluted 1:1 with balanced salt solution at RT and layered onto Ficoll Paque PLUS (GE Healthcare, #GE17-1440-02) followed by centrifugation at 400 g for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... 1% saline-sodium citrate [SSC]) at 50 °C and exposed to a phosphor screen (GE Healthcare, USA) overnight ...
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bioRxiv - Microbiology 2022Quote: ... 0.3 M sodium citrate pH 7.0) and transferred to an Amersham Hybond-N+ membrane (GE Healthcare Life Sciences) by capillary action overnight ...
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bioRxiv - Biochemistry 2021Quote: Fractions containing the dIG8-CC protein were then buffer-exchanged to buffer B (20mM Tris·HCl, 150 mM sodium chloride, pH 7.5) in a HiPrep 26/10 desalting column (GE Healthcare), and incubated overnight at 4°C with inhouse-produced His6-tagged TEV peptidase at a peptidase:substrate ratio of 1:20 (w/w ...
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bioRxiv - Immunology 2021Quote: ... Samples were buffer exchanged into NMR buffer (20 mM sodium phosphate pH 7.0, 50 mM NaCl, 0.02% NaN3) using PD10 desalting columns (GE Healthcare). Samples were concentrated further to a volume of ~300 μL ...