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Citations for GE Life Sciences :
101 - 150 of 921 citations for Annexin V Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... or anti-CSP repeat antibody – mAB 3D11 (Yoshida et al., 1980) and HRP bound antibodies (GE healthcare, Thermo Fischer Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... ActRIIB-Fc was isolated using affinity chromatography with Mab Select Sure Protein A (GE healthcare - Waukesha, WI), followed by dialysis into 10mM Tris ...
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bioRxiv - Bioengineering 2020Quote: ... 10% glycerol (v/v) at 4°C and re-loaded onto a Ni-NTA column (HisTrap™ HP, GE Healthcare). An imidazole gradient was applied (from 20mM to 500mM ...
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bioRxiv - Plant Biology 2021Quote: ... and the peptides used for SPR was dissolved in HBS-EP+ (10 mM HEPES, 150 mM NaCl, 3 mM EDTA and 0.05% [v/v] surfactant P20 (GE Healthcare)) ...
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bioRxiv - Biochemistry 2021Quote: ... 10% (v/v) glycerol and loaded on a HiLoad 16/10 Superdex 200 prep grade gel filtration column (GE Healthcare) equilibrated in the same buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... All cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Nacalai Tesque Inc.) supplemented with 10% v/v fetal bovine serum (FBS; Hyclone, GE Healthcare) and penicillin/streptomycin (Nacalai Tesque Inc. ...
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bioRxiv - Immunology 2023Quote: ... resuspended in HBSS and 20mM HEPES and loaded onto a 62% (v/v) Percoll gradient (GE Healthcare #17-0891-01) in HBSS and 20mM HEPES ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was spun down at 400× g for 5 minutes followed by resuspension in a 33.75% (v/v) Percoll (GE Healthcare) diluted in PBS with 2% foetal calf serum and density gradient centrifugation at 700 x g for 12 minutes ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was spun down at 400× g for 5 minutes followed by resuspension in a an isotonic 33.75% (v/v) Percoll (GE Healthcare) diluted in PBS with 2% foetal calf serum and density gradient centrifugation at 700x g for 12 minutes ...
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bioRxiv - Immunology 2023Quote: ... Blots were then wash 3 times in 15-30 mL TBST (0.05% v/v) and chemiluminescence were quantified on the Amersham Imager 680 (GE Healthcare). All immunoblots shown are representative of 3 or more biological replicates.
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bioRxiv - Plant Biology 2019Quote: ... and electrotransfered (2 h at 80 V or 16 h at 30 V and 4°C) onto polyvinylidene difluoride membrane (Amersham, GE Healthcare). The recombinant enzymes were probed using anti-V5-HRP conjugated antibody (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: ... and 5% (v/v) glycerol for further purification by anion exchange on a Mono Q 5/50 GL column (GE Healthcare) with a gradient from 50 mM to 1 M NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... 10% (v/v) glycerol and 1 mM DTT prior to ion exchange chromatography using a Fast Flow SP column (GE Healthcare). Elution from the SPFF column was performed with a 100 mM to 1M gradient over 20 column volumes ...
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bioRxiv - Plant Biology 2022Quote: ... The obtained pellet was resuspended in 2 mL of washing buffer and loaded on top of a 28% (v/v) Percoll (GE Healthcare) continuous gradient with a 0% to 4% (w/v ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted protein was then exchanged into lysis buffer supplemented with 0.15% w/v OGNG / 0.015% w/v CHS by passing over a pre-equilibrated Sephadex PD-10 desalting column (GE Life Sciences). TEV protease was added to the desalted protein at a weight ratio of 1:10 for overnight tag cleavage ...
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bioRxiv - Neuroscience 2020Quote: ... proteins were separated on 12.5% (v/v) acrylamide gels of 25 cm x 21 cm x 1 mm using an Ettan DALTsix electrophoresis unit (GE Healthcare). The SDS-PAGE was first run at a current of 2 W/gel for 1 h and then at a constant current of 15 W/gel for 5 h ...
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bioRxiv - Plant Biology 2022Quote: ... the gels were run in 1× RNA running buffer (1 × MOPS and 6% [v/v] formaldehyde) and blotted onto the Hybond-N+ membrane (GE Healthcare) soaked in 20× SSC (3.0 M NaCl and 0.3 sodium citrate) ...
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bioRxiv - Microbiology 2023Quote: ... Blots were developed with standard BCIP (5 % (w/v) in DMF) + 250 μL NBT (5 % (w/v) in 70 % DMF) or using the ECL western blotting reagent (GE Healthcare).
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bioRxiv - Immunology 2024Quote: ... HBS-P+ buffer (10 mM HEPES pH 7.4, 0.15 M NaCl, 0.05% v/v P20 surfactant, (GE Healthcare, Marlborough, MA, USA) was used as the running buffer ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... the recombinant protein was purified through 5 mL HisTrap HP column (GE Healthcare), and the eluted protein was further purified through 5 mL HiTrap Heparin HP column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: ... The recombinant proteins were purified through 5 mL HisTrap HP column (GE Healthcare), and the eluted protein was further purified through 5 mL HiTrap Q HP column (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... The recombinant proteins were first purified on a HisTrap HP column (GE Healthcare), followed by buffer exchange using HiPrep 26/10 desalting column (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... The recombinant proteins were purified by Ni+ sepharose 6 fast flow (GE Healthcare), glutathione Sepharose 4B (GE Healthcare ...
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bioRxiv - Neuroscience 2019Quote: ... Recombinant tau proteins were immobilized on the CM5 sensor chip (Biacore, GE Healthcare) via amine coupling ...
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bioRxiv - Immunology 2021Quote: ... Recombinant IgG was purified using HiTrap Protein A columns (GE Healthcare Life Sciences). For IgA1 or IgA2 antibodies ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The recombinant protein was purified through a nickel-nitrilotriacetic acid column (GE Healthcare) and subsequently loaded on a gel filtration column Sephacryl S-200 HR (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... Purified recombinant proteins were loaded onto Superdex 75 10/300 column (GE Healthcare) in 150mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... The recombinant proteins were purified by Ni2+-IMAC on HisTrap columns (GE Healthcare). Fractions ...
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bioRxiv - Immunology 2020Quote: ... The recombinant GST fusion proteins were affinity purified on GSH-Sepharose (GE Healthcare) from bacterial cultures incubated with 0.25 mM isopropyl-β-D-thiogalactopyranoside (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... Recombinant proteins were purified with a fast flow Ni-NTA column (GE Healthcare) and eluted by 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Microbiology 2023Quote: ... The recombinant CoxbHtrA protein was purified using an ÄKTA avant system (GE Healthcare) equipped with a 5 mL StrepTrap HP column (GE Healthcare) ...
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bioRxiv - Cell Biology 2023Quote: Recombinant RAD51 proteins were spotted on nitrocellulose membrane (GE Healthcare, Little Chalfont, UK). Membranes saturation ...
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bioRxiv - Immunology 2023Quote: ... Recombinant IgG was purified using HiTrap Protein A columns (GE Healthcare Life Sciences). To produce antibody Fabs ...
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bioRxiv - Microbiology 2024Quote: ... and recombinant protein was purified using glutathione sepharose beads (GE Healthcare Life Sciences).
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bioRxiv - Neuroscience 2024Quote: ... recombinant IgLON5 or LG1 protein was spotted onto a nitrocellulose membrane (GE Healthcare), membranes were blocked with 1% BSA in PBS ...
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bioRxiv - Immunology 2020Quote: ... clarified supernatants were passed over 2G12 mAb immobilized on CNBr-Activated Sepharose 4B resin (GE Healthcare; Cat# 17043001). Resin was washed with tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2020Quote: ... All mAbs were purified from cell culture supernatant using a HiTrap protein G HP affinity column (GE Healthcare) and stored at −80 °C ...
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bioRxiv - Immunology 2021Quote: ... and Ni Sepharose™ 6 Fast Flow (50 µL per 1 mg digested mAb; GE Healthcare Life Sciences) which were washed twice with PBS before adding to the reaction mixture ...
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bioRxiv - Immunology 2021Quote: ... and Ni Sepharose™ 6 Fast Flow (50 μL per 1 mg digested mAb; GE Healthcare Life Sciences) which were washed twice with PBS before adding to the reaction mixture ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... to a polyvinylidene difluoride membrane and blocked in TBST buffer (TBS with 0.1% (v/v) Tween-20) supplemented with 1% ECL Advance Blocking Agent (GE Healthcare, Waukesha, WI) for 40 minutes at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... 10% (v/v) glycerol and 1 mM DTT) and injected onto a MonoQ 5/50 GL column (GE Healthcare and Life Sciences) at 1 mL/min ...
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bioRxiv - Microbiology 2019Quote: ... Prior to infection HFFs were grown to confluency in DME supplemented with 10% v/v cosmic calf serum (GE Healthcare, New Zealand) (D10) ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5 % (v/v) glycerol by FPLC (ÄKTA explorer, Amersham Pharmacia Biotech) using a Superdex™ 200pg 16/60 FPLC column (GE Healthcare). Final protein fractions were analyzed by SDS-PAGE electrophoresis.
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bioRxiv - Cell Biology 2021Quote: ... 10% (v/v) glycerol and 1 mM DTT) and injected onto a MonoQ 5/50 GL column (GE Healthcare and Life Sciences) at 1 mL/min ...
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bioRxiv - Microbiology 2022Quote: ... The leukocyte-depleted red blood cell pellet was then resuspended in 10 ml PBS and 5 ml were carefully layered on 6 ml of 70% (v/v) isotonic Percoll cushion (GE Healthcare, UK). After centrifugation at 1200 x g for 15 mins ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein solution was diluted 1:10 v/v with 20 mM Tris 8.0 and further purified with anion exchange chromatography (HiTrapQ column, GE Healthcare Life Sciences) using a NaCl gradient of 50 mM to 500 mM in 20 mM Tris 8.0 ...
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bioRxiv - Plant Biology 2021Quote: ... 1% (w/v) dithiothreitol (DTT; GE Healthcare, Piscataway, USA), and 10 μL of EDTA-free Halt protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... and analysed with ImageQuant TL (v. 7.0, GE Healthcare). For re-probing ...
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bioRxiv - Cancer Biology 2023Quote: ... controlled by the SoftWoRx software v.6.5.2 (Applied Precision) and a 60x objective lens with a CoolSNAP HQ2 camera (Photometrics) ...
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bioRxiv - Microbiology 2020Quote: ... Human mAbs were purified by loading the supernatant onto a 1 mL Protein A HP HiTrap column (GE Healthcare). Columns were equilibrated and washed using Dulbecco’s phosphate buffered saline (DPBS) ...