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Citations for GE Life Sciences :
1 - 50 of 306 citations for Alpinone 3 Acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... separated by SDS-PAGE using a 3-15% Tris-Acetate gel and transferred to nitrocellulose membranes (GE Healthcare). Densitometry analysis was performed by the Image J software (National Institutes of Health).
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bioRxiv - Cell Biology 2023Quote: ... Cellulose acetate membrane (0.2μm pore, GE Healthcare) was equilibrated in 0.1% SDS/H2O and fixed to the filter trap device (PR648 Slot Blot Blotting Manifold ...
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bioRxiv - Immunology 2022Quote: ... Aliquots of the purified A48 or D48 PTX3 proteins were run under denaturing conditions on Tris-Acetate 3–8% (w/v) and Bis-Tris 10% (w/v) protein gels (GE Healthcare Life Sciences), in the absence and presence ...
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bioRxiv - Molecular Biology 2021Quote: ... using 10mM sodium acetate pH4.0 solution (GE Healthcare). The surface of the sensor chip was blocked with 1M ethanolamine-HCl pH8.5 (GE Healthcare) ...
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bioRxiv - Plant Biology 2022Quote: ... A cellulose acetate membrane filter (GE Healthcare Life Sciences) was placed in a slot blot apparatus (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... A cellulose acetate membrane (0.2 µm pore size, GE Healthcare) was pre-equilibrated in 0.1% SDS and affixed to the filter trap apparatus (PR648 Slot Blot Blotting Manifold ...
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bioRxiv - Neuroscience 2020Quote: ... containing a 0.2 μM cellulose acetate membrane (Whatman, GE Healthcare) as described in (Torres et al. ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins in 10 mM acetate buffer (pH 4.5, GE Healthcare) was then injected over the chip surface at a flow rate of 30 μl/min to couple the amino groups of the recombinant proteins to the carboxymethyl dextran matrix ...
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bioRxiv - Neuroscience 2020Quote: ... at 10-20 μg/mL concentration in 10 mM acetate solution (GE Healthcare) at pH 4.5 to reach a final surface ligand density of around 2000-4000 response units (RUs) ...
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bioRxiv - Neuroscience 2023Quote: ... at 10–20 μg/mL concentration in 10 mM acetate solution (GE Healthcare) at pH 4.5 to reach a final surface ligand density of around 2000–4000 response units (RUs) ...
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bioRxiv - Biochemistry 2022Quote: ... The ammonium acetate was removed using a PD SpinTrap G-25 column (GE Healthcare); the sample was eluted in G-buffer without ammonium acetate.
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bioRxiv - Neuroscience 2020Quote: ... containing a cellulose acetate membrane with pores with a 0.2 μm diameter (Whatman, GE Healthcare) as described in (Torres et al. ...
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bioRxiv - Bioengineering 2022Quote: ... The supernatant was filtered through a 0.45 mm cellulose-acetate filter (GE Healthcare, Little Chalfont, UK) and subsequently used for further purification.
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bioRxiv - Biochemistry 2020Quote: ... and equal amounts of lysates were filtered through a 0.2 μm pore size cellulose acetate membrane (GE Healthcare) and washed with lysis buffer ...
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bioRxiv - Cell Biology 2021Quote: ... 10 µg of proteins were resolved in Tris-acetate SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare). For extracellular vesicle fractions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA was then precipitated in ethanol/ammonium acetate and purified by G25 size exclusion chromatography (illustraTMNAPTM-10, GE Healthcare). RNA purity was assayed by denaturing gel electrophoresis.
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bioRxiv - Microbiology 2022Quote: ... the purified paLptDE or sfLptDE protein samples were buffer exchanged into 150 mM ammonium acetate (pH 8.0) containing 0.5% C8E4 (v/v) on a Superdex 200 gel filtration column (GE Healthcare). LptDE samples were subsequently introduced via gold-coated nanospray capillaries prepared in house and MS spectra were recorded on a Synapt G2-Si instrument (Waters ...
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bioRxiv - Cancer Biology 2021Quote: ... pH 3-10 (GE Healthcare) and peptides focused for 20 kVh ...
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bioRxiv - Cancer Biology 2021Quote: ... pH 3-10 (GE Healthcare) diluted 1:50 in 5% glycerol ...
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bioRxiv - Microbiology 2020Quote: ... Pure FasR and FasRF123A were dialysed against 10 mM sodium acetate pH 4.5 and coupled to a CM5 sensor chip using the Amine Coupling Kit (GE Healthcare). Micromolar concentrations of C20-CoA were dialysed against 25 mM Tris.HCl pH 8 ...
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bioRxiv - Biochemistry 2020Quote: ... The reaction was stopped by 20-fold dilution in 50 mM sodium acetate (pH 4.5) and chains of different lengths were separated by cation exchange using a Resource S column (GE Healthcare). Elution of different chain lengths was achieved with a gradient from 0 to 600 mM NaCl.
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 RBD was diluted to a concentration of 15 μg/mL with sodium acetate (pH 4.5) and immobilized on a CM5 chip (GE Healthcare) at a level of ∼150 response units (RU) ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant and the crude lysate were separately filtered using a 0.2 μm syringe Filter (Puradisc FP 30 Cellulose Acetate Syringe Filter, GE Healthcare) and directly loaded onto the column at 1 mL/min one after another ...
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bioRxiv - Immunology 2022Quote: ... Nb-Fc was diluted to a concentration of 5 μg/ml with sodium acetate (pH 4.5) and immobilized on a CM5 chip (GE Healthcare) at a level of ∼150 response units ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples containing 10 µg of proteins were filtered through Whatman Cellulose Acetate Membrane Filters with 0.2 µm pore sizes (GE Healthcare), which have very low protein binding capacity ...
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bioRxiv - Biochemistry 2023Quote: ... The respective reactions were stopped by 20-fold dilution in 50 mM sodium acetate (pH 4.5) and chains of different lengths were separated by cation exchange using a Resource S column (GE Healthcare). Elution of different chain lengths was achieved with a gradient from 0 to 600 mM NaCl.
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bioRxiv - Biochemistry 2024Quote: ... The respective reactions were stopped by 20-fold dilution in 50⍰mM sodium acetate (pH 4.5) and chains of different lengths were separated by cation exchange using a Resource S column (GE Healthcare). Elution of different chain lengths was achieved with a gradient from 0 to 600⍰mM NaCl.
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bioRxiv - Bioengineering 2024Quote: ... was diluted to 50 μg/mL in 10 mM acetate buffer at pH 5.0 and immobilized on the surface of a CM5 sensor chip (GE Healthcare 29149604) using amine coupling chemistry ...
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bioRxiv - Microbiology 2021Quote: ... or pGEX-6P-3 (GE Healthcare). Pc3566 (from the −35 box to the TSS ...
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bioRxiv - Microbiology 2023Quote: ... 1.04% PharmalyteTM 3 – 10 (GE Healthcare), 50 mM DTT and trace amounts of bromophenol blue) ...
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bioRxiv - Cell Biology 2021Quote: ... 5nmole of Nedd8 or LC3B [in acetate buffer (pH: 4.0)] was immobilized on a CM5 sensor chip (GE Healthcare Life Sciences, 29149604) by amine coupling ...
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bioRxiv - Plant Biology 2023Quote: ... for 2 hours at 45°C and then desalted against 50 mM ammonium acetate using a PD MidiTrap G-10 column (size exclusion limit: 700 Da; GE Healthcare, Sweden) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... 100 µg of freeze-dried HMW compounds were dissolved in 1 mL of 20 mM sodium acetate buffer (pH 4.5) and eluted through a 1 ml HiTrap ANX FF column (GE Healthcare, 17-5162-01). Samples were eluted at a flow rate of 1 ml/min with 20 mM sodium acetate buffer pH 4.5 for 36 ml ...
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bioRxiv - Neuroscience 2023Quote: ... pH 3–10 (GE Healthcare Life Sciences), and bromophenol blue were added and centrifuged (14,000 rpm for 10 min ...
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bioRxiv - Immunology 2022Quote: ... 3 ml Ficoll-Paque media (GE Healthcare) was pipetted into the bottom and the diluted 4 ml blood sample was carefully layered on top ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... CG42797EcoRI_F: 5’-CCGgaattcATGGAGCCACCAGCT-3’ and CG42797XhoI_R: 5’-TAGAGGTACCctcgagCTACTCAATGCCGAACGTGTTG-3’ and cloned by enzymatic digestion into a pGEX6P1(GE Healthcare).
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... CG42797EcoRI WWI_F: 5’-ccgGAATTCCCACCATTGCCGCCTG-3’ CG42797XhoI WWII_R: 5’-ccgCTCGAGTCAACGAGGATCCATGAA-3’ and cloned by enzymatic digestion into a pGEX6P1(GE Healthcare).
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... CG42797EcoRI_F: 5’-CCGgaattcATGGAGCCACCAGCT-3’ and CG42797XhoI_Nterm_R: 5’-CCGctcgagTCATTCGCTGGGCTGC-3’ and cloned by enzymatic digestion into a pGEX6P1(GE Healthcare).
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bioRxiv - Molecular Biology 2023Quote: Mouse cortical and hippocampal brain homogenates were filtered through a cellulose acetate membrane (10 µg per dot) with a pore size of 0.2 µm (GE Healthcare Life Sciences, Munich, Germany). Per cavity of filtration unit ...
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bioRxiv - Biochemistry 2022Quote: ... and then immobilized in 7 cm IPG strips (pH 3-10 : 70×3×0.5 mm) and a linear gradient (NL) (GE Healthcare Immobiline™ Dry Strip IPG ...
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bioRxiv - Immunology 2023Quote: ... [3] A HisTrap FF column (Cytiva, GE Healthcare) was used in the Äkta Pure protein chromatography system (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... was amplified by PCR using the primers (5’-GGTTCCGCGTGGATCCATGTCTCATGCAGCCGAGCCA-3’ and 5’-GGAATTCCGGGGATCCTCAGGACTCCTCTTCAATGCTGA-3’) and cloned into BamHI site of pGEX-4T-1 expression vector (GE Healthcare) using In-Fusion HD Cloning System (Clontech) ...
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bioRxiv - Biochemistry 2021Quote: ... and the cleaved DRH-3 CTD and DRH-3 NTD were purified using a Superdex 75 HiLoad 16/600 size-exclusion column (GE Healthcare). Peak fractions were pooled and concentrated to 5-15 mg ml−1 in a buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The sample was then run on an 18 cm 3-10 strip using the Ettan IPGphor 3 Isoelectric Focusing System (GE Healthcare). Four voltage steps (50 V for 10 hours ...
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bioRxiv - Cancer Biology 2022Quote: Custom siRNAs targeting 5’-CACCAUGAGUGGCAGUCAG-3’ 33 for FRA1 and 5′-GUUCACUGCUGGCCUAUAA-3’ 34 for FLI1 were ordered from GE Healthcare Dharmacon ...
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bioRxiv - Biochemistry 2021Quote: ... PKAc was immobilized via standard N-hydroxysuccinimide (NHS) / 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) amine coupling on a CM5 (carboxyl methyl dextran) sensor chip (GE Healthcare). Before covalent immobilization of PKAc ...
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bioRxiv - Microbiology 2021Quote: ... using a mixed solution of 200 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and 50 mM N-hydroxysuccinimide (NHS) in a Biacore T-200 SPR instrument (GE Healthcare), reaching 1000 response units (RU) ...
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bioRxiv - Neuroscience 2023Quote: ... the lysates were adjusted to equal protein concentrations and incubated with 500 pmol of GST-14-3-3 immobilised on glutathione-sepharose 4B beads (GE Healthcare) for 60 min at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: Dried blood spot (DBS) samples were collected on Whatman 3-mm filter paper (Whatmann No. 3, GE Healthcare Life Sciences, PA, USA) from enrolled patients on Day 0 (before treatment ...
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bioRxiv - Molecular Biology 2020Quote: ... and subcloned into the pGEX-6P-3 (GE Healthcare).