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Citations for GE Life Sciences :
1 - 50 of 1931 citations for Alpha Cyclodextrin Solution 5% w v since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... a Ficoll solution (50% w/v Ficoll PM400, GE Healthcare, in 50 mM HEPES pH 7.4) was combined 1:1 with the KH buffer and microinjected into the cells as described for the isosmotic condition ...
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bioRxiv - Microbiology 2023Quote: ... Blots were developed with standard BCIP (5 % (w/v) in DMF) + 250 μL NBT (5 % (w/v) in 70 % DMF) or using the ECL western blotting reagent (GE Healthcare).
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bioRxiv - Zoology 2020Quote: ... and 3500 V/2 mA/5 W for 6 h using a Multiphor II system (GE Healthcare). The IEF strip was then equilibrated for 15 min in 5 ml equilibration stock solution (6 M urea ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted protein was then exchanged into lysis buffer supplemented with 0.15% w/v OGNG / 0.015% w/v CHS by passing over a pre-equilibrated Sephadex PD-10 desalting column (GE Life Sciences). TEV protease was added to the desalted protein at a weight ratio of 1:10 for overnight tag cleavage ...
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bioRxiv - Plant Biology 2021Quote: ... 1% (w/v) dithiothreitol (DTT; GE Healthcare, Piscataway, USA), and 10 μL of EDTA-free Halt protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Aliquots of the purified A48 or D48 PTX3 proteins were run under denaturing conditions on Tris-Acetate 3–8% (w/v) and Bis-Tris 10% (w/v) protein gels (GE Healthcare Life Sciences), in the absence and presence ...
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bioRxiv - Cell Biology 2020Quote: ... 5% glycerol (w/v, 0.1% Triton X-100) and mixed with 50 µl Glutathione Sepharose 4 Fast Flow (GE Healthcare) slurry ...
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bioRxiv - Immunology 2022Quote: ... Membranes were then incubated with the corresponding HRP-tagged secondary antibodies in BSA (5% w/v) PBS-T for 1 hour before visualisation using Amersham ECL prime detection reagent (GE healthcare) and a G:Box Chemi XX6 system (Syngene) ...
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bioRxiv - Plant Biology 2023Quote: ... the thalli were treated with 75.5% (w/v) iohexol (GE Healthcare Pharma) for 1 h and mounted on glass slides following the iTOMEI protocol (Sakamoto et al. ...
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bioRxiv - Plant Biology 2023Quote: ... the samples were infiltrated with 75.5% (w/v) iohexol (GE Healthcare Pharma) for 1 h and mounted onto slides ...
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bioRxiv - Plant Biology 2023Quote: ... the samples were soaked in 75.5% (w/v) iohexol (GE Healthcare Pharma) for 1 h and mounted onto slides ...
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bioRxiv - Microbiology 2024Quote: ... Cells were separated by a 40/80% (w/v) Percoll (GE Healthcare) density gradient and washed prior to staining for flow cytometry analysis ...
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bioRxiv - Biochemistry 2020Quote: ... 5% (v/v) glycerol using a HiTrap Desalting column (GE Healthcare). All protein samples were aliquoted and stored at −80 °C.
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bioRxiv - Genomics 2021Quote: ... consisting of 6.7% (w/v) Ficoll PM-400 (GE Healthcare, #17-0300-05), 225 mM Tris pH 7.5 (Teknova ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The isolated tumor infiltrating immunce cells were centrifuged in 35% v/v Percoll solution (GE Healthcare) and RPMI (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... 10% w/v glycerol and applied to a MonoS or Capto S column (GE Healthcare). Protein was eluted with a gradient to 1.0 M NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... and 5% (v/v) glycerol for further purification by anion exchange on a Mono Q 5/50 GL column (GE Healthcare) with a gradient from 50 mM to 1 M NaCl ...
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bioRxiv - Plant Biology 2020Quote: ... separated in 1% [w/v] agarose gels and blotted onto Amersham Hybond-XL membranes (GE Healthcare) according to the supplier’s recommendations ...
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bioRxiv - Immunology 2020Quote: ... Leucocytes enrichment was then performed through a 40/80% (w/v) Percoll density gradient (GE Healthcare) centrifuged for 15min at 1900g at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... 10% (w/v) glycerol) by size exclusion chromatography using a Superdex200 16/600 column (GE Healthcare). Aliquots were flash frozen in liquid nitrogen and stored at −80°C until further use ...
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bioRxiv - Genomics 2023Quote: ... 0.05% (w/v) Tween 20) and 50 µl of SpeedBeads™ magnetic carboxylate modified particles (GE Healthcare 65152105050250 ...
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bioRxiv - Synthetic Biology 2020Quote: ... the plate was blocked with 200 μl of a 2% (w/v) ECL Advance Blocking Reagent (GE Healthcare) solution in PBS-T (PBS supplemented with 0.1% (v/v ...
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bioRxiv - Plant Biology 2021Quote: ... separated by gel electrophoresis in 0.7% (w/v) agarose gels and blotted onto Hybond N nylon membranes (GE Healthcare). A ~500 bp probe was produced by PCR amplification (primer pair P19/P20 ...
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bioRxiv - Biochemistry 2024Quote: ... 0.05% (w/v) bromophenol blue and analysed by polyacrylamide gel electrophoresis and phosphorimaging on a Typhoon-Trio (GE Healthcare), as described in (41).
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bioRxiv - Immunology 2023Quote: ... Supernatant was spun down at 400× g for 5 minutes followed by resuspension in a 33.75% (v/v) Percoll (GE Healthcare) diluted in PBS with 2% foetal calf serum and density gradient centrifugation at 700 x g for 12 minutes ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was spun down at 400× g for 5 minutes followed by resuspension in a an isotonic 33.75% (v/v) Percoll (GE Healthcare) diluted in PBS with 2% foetal calf serum and density gradient centrifugation at 700x g for 12 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... except with 0.025% (w/v) GDN as the detergent and gel filtration with a Superose 6 Increase 10/300 column (GE Healthcare) following affinity purification.
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bioRxiv - Biophysics 2020Quote: ... The complex was then incubated with a large excess of nanobody 4C8 (in PBS buffer with 0.08% (w/v) digitonin) and injected on a Superdex200 10/300increase column (GE healthcare) pre-equilibrated in buffer E ...
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bioRxiv - Biophysics 2022Quote: ... pre-equilibrated with buffer (50 mM HEPES pH 7.4 and 0.08% [w/v] GDN) was used to exchange CIV into GDN using an Äkta Pure M25 chromatography system (GE Healthcare) operated at 4°C with UV detection at 280 nm and 420 nm ...
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bioRxiv - Molecular Biology 2020Quote: ... After washing the membranes four times for 5 min at room temperature in Washing buffer (PBS, 0.1% (w/v) Tween-20) appropriate HRP-coupled secondary antibodies (anti-mouse IgG (NA931, GE Healthcare), anti-rabbit IgG (NA934 ...
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bioRxiv - Genomics 2023Quote: ... and lysed by resuspension in 20 ml cold F Buffer (18% w/v Ficoll-PM400 (GE Healthcare 17-0300-50), 40 mM potassium phosphate ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were separated by an 8% (w/v) polyacrylamide gel containing 0.3% SDS and detected using a Typhoon 9400 phosphorimaging system and ImageQuant software (GE Healthcare).
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... 293-F cells were seeded 2-3 h before transfection at a density of 1×106 cells per ml in Freestyle medium supplemented with 0.2% w/v HyClone Cell Boost (GE Healthcare, SH30584.02). For transient transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction products were resolved on 6–8% (w/v) denaturing polyacrylamide gels and visualized with Typhoon FLA scanner (GE Healthcare). In total ...
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bioRxiv - Plant Biology 2021Quote: ... and proteins were separated by SDS/PAGE on 8-10% (w/v polyacrylamide) gels and transferred to PVDF membranes (Semi-Dry-Blot, GE Healthcare). After transfer ...
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bioRxiv - Immunology 2020Quote: ... boiled and separated by gel electrophoresis in Tris-glycine SDS (TGS) buffer (20 mM Tris, 192 mM glycine, 1 % (w/v) SDS) and transferred to a nitrocellulose membrane (GE Healthcare) in Tris glycine (TG ...
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bioRxiv - Bioengineering 2023Quote: ... Equal Volume of total soluble proteins were separated on a 15% (w/v) SDS polyacrylamide gel and transferred onto a Hybond-P membrane (RPN303F, GE Healthcare). Nonspecific binding was blocked with 3% (w/v ...
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bioRxiv - Cancer Biology 2023Quote: ... and separated by 4-12% Bis-Tris SDS-PAGE electrophoresis (at 180 V, 300 W, 55 min) and transferred onto a nitrocellulose membrane (GE Healthcare). Membranes were blocked in 5% BSA(Sigma ...
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bioRxiv - Microbiology 2022Quote: ... 6 μg of total RNAs were resolved on a 1 % (w/v) agarose gel then transferred onto Amersham Hybond-N+ Membrane (GE Healthcare) by capillary transfer ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were probed with primers 32P-labelled with [alpha-P32]Deoxycytidine 5’-triphosphate (dCTP) using Megaprime DNA labelling system (GE Healthcare) (for primers ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5 % (v/v) glycerol by FPLC (ÄKTA explorer, Amersham Pharmacia Biotech) using a Superdex™ 200pg 16/60 FPLC column (GE Healthcare). Final protein fractions were analyzed by SDS-PAGE electrophoresis.
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bioRxiv - Cell Biology 2021Quote: ... 10% (v/v) glycerol and 1 mM DTT) and injected onto a MonoQ 5/50 GL column (GE Healthcare and Life Sciences) at 1 mL/min ...
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bioRxiv - Microbiology 2022Quote: ... The leukocyte-depleted red blood cell pellet was then resuspended in 10 ml PBS and 5 ml were carefully layered on 6 ml of 70% (v/v) isotonic Percoll cushion (GE Healthcare, UK). After centrifugation at 1200 x g for 15 mins ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the sections were collected on coverslips (22 mm x 22 mm) previously pretreated with a silanization solution (0.3% (v/v) bind-silane (GE Healthcare Life Sciences, 17-1330-01), 0.1% (v/v ...
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bioRxiv - Genetics 2024Quote: ... without Alpha-Thioglycerol (GE Healthcare Life Sciences Cat. # SH30228.01); 1X GlutaMAX-I ...
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bioRxiv - Cell Biology 2023Quote: ... estimated by Bradford assays) were separated in a 10 % (w/v) polyacrylamide gel and then transferred on PVDF membranes (GE Healthcare Life Sciences, Amersham). Immunodetection was performed with an anti-HA monoclonal antibody [HA Epitope Tag (YPYDVPDYA ...
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bioRxiv - Cell Biology 2024Quote: ... estimated by Bradford assays) were separated in a 10 % (w/v) polyacrylamide gel and were transferred on PVDF membranes (GE Healthcare Life Sciences Amersham). Immunodetection was performed with an anti-FLAG antibody (Agrisera ...
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bioRxiv - Biophysics 2022Quote: ... 89% (v/v) Percoll (GE healthcare), 1 cOmplete™ EDTA-free Protease Inhibitor ...
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bioRxiv - Microbiology 2020Quote: ... 0.005% (v/v) P20 (GE Healthcare), pH 6.5 ...
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bioRxiv - Biophysics 2020Quote: ... 1 mM CaCl2 then incubated with 1:50 (w/w) PreScission Protease (GE Healthcare) for 18 hrs at 4 °C ...