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Citations for GE Life Sciences :
401 - 450 of 641 citations for African Swine Fever virus pp62 polyprotein His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... The bacterial lysate was clarified using centrifugation at 20,000 x g for 40 min and loaded onto a 5 ml His-Trap column (GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Microbiology 2019Quote: ... The recovered supernatant was filtered using 0.45 μm syringe-driven filters (Jet Biofil) and loaded in a His-Trap column (GE Healthcare) equilibrated with 350 mM NaCl ...
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bioRxiv - Cell Biology 2019Quote: ... The sample was concentrated on an Amicon Ultra Centrifugal Filter Unit (10 kDa cut-off) and injected at 0.5 ml/min on a Hi-Load 16/60 Superdex column (GE healthcare), equilibrated with 20 mM Tris-HCl buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... the lysate spun down at 12,000 x g for 30 min and then loaded onto a 5 ml His-Trap Crude FF column (GE Healthcare) using a ÄKTA Purifier protein purification system (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... The resulting lysate was centrifuged for 30 minutes at 20,000 rpm to remove cell debris and the supernatant was filtered with a 0.45 μm filter before loading onto an equilibrated 5 ml His Trap HP column (GE healthcare) connected to an ÄKTA pure 25 system ...
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bioRxiv - Biochemistry 2021Quote: ... XLF-6xHis was purified using a HP His-trap column followed by a HiTrap Q HP column (both GE Healthcare). mSUMO1 (#UL-712) ...
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bioRxiv - Systems Biology 2021Quote: ... The supernatants obtained after this step were loaded in 5-mL His-trap columns (GE Healthcare Systems, Chicago, IL, USA) pre-equilibrated with buffer A supplemented with 100 mM NaCl and 20 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... the obtained supernatants were filtered through sterile PVDF filters (pore size 0.22 μm) and loaded on 1 ml His-Trap columns (GE Healthcare), pre-equilibrated with ten column volumes of each Millipore water and sodium phosphate buffer A ...
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bioRxiv - Microbiology 2020Quote: ... His-CD147 (produced by our laboratory) was fixed to the surface of CM5 sensor chips (GE Healthcare Bio-Sciences AB) by amino coupling kit (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... His-Bro1ΔAPRR purified from BL21-DE3 using Ni2+-affinity chromatography (5 ml HiTrap Chelating FF column; GE Healthcare, United Kingdom). GST-Bro1V(370-709 ...
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bioRxiv - Immunology 2021Quote: ... His-CyPA (produced by our laboratory) was fixed to the surface of CM5 sensor chips (GE Healthcare Bio-Sciences AB) by an Amine Coupling Kit (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... Insoluble material was sedimented by centrifugation at 13,500 g for 30 min at 4°C prior to loading on His-trap FF columns (GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was applied to a 5-ml His-Trap HF column charged with NiCl2 (GE Healthcare, 17-5248-02). GFP-NONO_WT or GFP-NONO_ΔRRM1 was eluted at room temperature with an imidazole gradient (25-500 mM ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was supplemented with 50 mM imidazole and loaded onto a 5 mL His-Trap FF column (GE Healthcare), washed with 6 column volumes (CV ...
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bioRxiv - Microbiology 2020Quote: ... Bacteria were pelleted and lysed by sonication in lysis buffer (Tris 100mM, NaCl 0.5M, glycerol 5%) and eGFP was purified using fast flow His-affinity columns (GE Healthcare) and eluted with 500 mM imidazole ...
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bioRxiv - Biochemistry 2020Quote: The purified rhGFAT2-his protein was subjected to a size exclusion chromatography using a Superdex 200 column (GE Healthcare, USA). The column was equilibrated with 1 CV of 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... The protein was purified via a two-step protocol of Ni-affinity using a 1ml His-Trap column (GE Healthcare) in Ni-affinity buffer [50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... the proteins were first purified from bacterial cell lysates using His-Trap HP (Ni-NTA) columns (GE Healthcare Life Sciences), as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble fraction was filtered through a 0.22-μm filter and loaded onto a 5 mL His Trap HP column (GE Healthcare) pre-equilibrated with buffer A (10 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: ... the cultures were spun down at 12,000 g for 30 min and the supernatants were loaded onto a His-Select Nickel Affinity gel (GE Healthcare). Recombinant PstS proteins were eluted with the elution buffer (500 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were further purified by size exclusion chromatography (Hi-load 16/60 Superdex 200 column, GE Healthcare Life Sciences) in a buffer (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Supernatant was first subjected to immobilized metal affinity chromatography (IMAC) using a 5mL Hi-Trap TALON column (GE healthcare sciences). The running buffer for IMAC was buffer TA ...
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A bacterial signal transduction phosphorelay in the methanogenic archaeon Methanosarcina acetivoransbioRxiv - Microbiology 2021Quote: ... Recombinant His-tagged proteins (MA4376, MA4376D54N and MA4375) were purified with the ÄKTAprime plus system using HisTrap HP (GE healthcare) columns ...
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bioRxiv - Microbiology 2020Quote: ... Clarified cell lysates (following 40 min centrifugation at 11,000 x g and 0.2 μm filtration) were loaded onto prepacked columns (1 ml) of Ni-Sepharose media (His trap, GE Healthcare) using a benchtop peristaltic pump ...
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bioRxiv - Microbiology 2022Quote: ... We pelleted and lysed bacteria by sonication in lysis buffer (Tris 100mM, NaCl 0.5M, glycerol 5%) and eGFP was purified using fast flow His-affinity columns (GE Healthcare) and eluted with 0.5 M imidazole ...
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bioRxiv - Microbiology 2023Quote: ... Pooled His-A51R factions were run over a Superdex 200 Increase 10/300 size-exclusion chromatography (SEC) column (GE Healthcare), exchanged into Storage Buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... Both anti-His and anti-Strep immunoblots were developed using Amersham ECL immunoblotting reagents (GE Healthcare Life Sciences, Buckinghamshire, UK).
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bioRxiv - Cell Biology 2022Quote: ... the lysate was centrifuged at 45,000 g for 30 min and His-(MBP-) ZNF524 further purified on a HisTrapTM HP (GE Healthcare) via the Akta Prime Plus System ...
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bioRxiv - Cell Biology 2023Quote: Both the GST-bait and His-prey lysates were incubated with Glutathione Sepharose 4B (GE Healthcare/Cytiva, 17-0756-01) for 30 min at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was filtered through a MF-Millipore Membrane Filter (0.45 µm pore size) then loaded on a 5 mL His-Trap HP column (GE Healthcare) pre-equilibrated with buffer A and CSE was eluted with a gradient from 20 mM to 250 mM imidazole in buffer A ...
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bioRxiv - Biochemistry 2023Quote: ... The elutate was further gel filtered over a Hi-Load 16/60 Superdex-200 pg column (GE Life Sciences, Cytiva) in buffer containing 50 mM Tris pH 7.5 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the lysate was ultracentrifuged at 22 000 g for 35 min at 4°C and the supernatant was filtered on 0.22 mm filters and then loaded on a His-Trap 5mL column (GE Healthcare) equilibrated with 50 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2024Quote: ... The lysate was clarified by centrifugation at 47,000 x g for 45min and applied to 5 ml His-Trap columns (GE Healthcare). After washing with a buffer excluding urea ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was filtered in 0.45 µm filter and loaded into a 5 ml His-trap FF column (GE Healthcare) pre-equilibrated with buffer A (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... Protein was purified via its C-terminal green fluorescence protein (GFP) tag using GFP nanobody coupled Sepharose Beads (GE Healthcare) and eluted by removing the GFP tag with the PreScission Protease ...
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bioRxiv - Biochemistry 2020Quote: ... The hexahistidine plus MBP tag was removed by TEV protease digestion followed by a second step HisTrap column (GE Healthcare). Protein was further purified using Q and Superdex G75 columns (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... at 4 °C to remove the SBP-tag and polished by size exclusion chromatography on a Superdex 200 Increase 3.2/300 column (GE Healthcare) equilibrated in 50 mM HEPES-NaOH ...
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bioRxiv - Biochemistry 2020Quote: ... The His6-tag-containing FdR9 was purified by affinity chromatography on an Äkta prime FPLC system (GE Healthcare, Freiburg, Germany), using a 5 mL HisTrap column (GE Healthcare) ...
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bioRxiv - Biophysics 2021Quote: ... The cleaved His6-MBP-tag was removed by gel filtration using a HiLoad 16/60 Superdex 200 column (GE healthcare) equilibrated in size exclusion buffer (20 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... The tag-less protein was then purified by size-exclusion chromatography (SEC) using a Superdex 200 increase column (GE Healthcare) equilibrated with a buffer containing 25 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... The GST tag of N-terminal GST-6xHis-RhoGAP domain was removed by addition of PreScission protease (GE healthcare Lifesciences) directly to the glutathione beads followed by incubation overnight at 4°C to obtain His-tagged RhoGAP ...
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bioRxiv - Immunology 2020Quote: ... to remove the GST tag and further purified by gel filtration (SuperoseTM 6 prep grade XK 16/70; GE Healthcare). To prepare for crystallization trials ...
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bioRxiv - Genetics 2020Quote: ... followed by purification of the tag-free protein by anion exchange chromatography on a HiTrap Q XL column (GE Healthcare) and nickel affinity chromatography ...
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bioRxiv - Biophysics 2023Quote: ... Protein was purified via its C-terminal green fluorescence protein (GFP) tag using GFP nanobody-coupled Sepharose beads (GE Healthcare) and eluted by removing the GFP tag with PreScission Protease ...
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bioRxiv - Biophysics 2023Quote: ... Pol II (with a protein A tag in the Rpb3 subunit) was purified by an IgG affinity column (GE Healthcare), followed by Hi-Trap Heparin (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... The eluted Cas1 without tag was concentrated and further purified by gel filtration chromatography (Superdex200 10/300 GL, GE Healthcare) in buffer B containing 10 mM Tris pH 8.0 ...
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bioRxiv - Bioengineering 2024Quote: Single-cycle kinetics analysis of CD4-Ig binding to the 16055-ConM-v8.1 SOSIP with C-terminal Spy-tag-2 (ST2) was performed at 25°C on a Biacore T100 (GE Healthcare). 1X HBS-EP containing 10 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... cells were treated with trypsin to remove any virus that had not entered the cell and then cushioned onto Ficoll-Pacque (GE Healthcare Life Sciences) to remove residual virus and debris ...
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bioRxiv - Biochemistry 2021Quote: ... and the released virus particles were purified by 55% cesium chloride and desalted through a PD10 column (GE Healthcare Life Sciences, Cat#17085101). Adenovirus particles were tail vein injected at doses of 0.1 OD for the lean ...
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bioRxiv - Neuroscience 2023Quote: ... The adeno-associated virus (AAV) vectors were produced by helper-free triple transfection and purified using affinity chromatography (GE Healthcare, Chicago, IL, USA). For production of AAV2.1 vector ...