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Citations for GE Life Sciences :
201 - 250 of 4567 citations for African Swine Fever Virus P72 Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... protein was again passed over Nickel resin to remove His-tagged protein and further purified by size exclusion chromatography (Superdex 75 10/300g, GE healthcare) into 20mM Tris.HCl 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... The 6×His-tagged SUMO fusion proteins were purified using a HisTrap™ HP Ni2+ affinity column (GE Healthcare Life Sciences). The fractions containing fusion protein were pooled and exchanged into a SUMO protease cleavage buffer (20 mM Tris-HCl at pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: SARM1-dN tagged with strep-tag II and flag-tag was immunoprecipitated with StrepTactin resin (GE healthcare), washed with buffer W (100 mM Tris-HCl pH8.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... NP_068576.1) with C-terminal Fc tag was expressed in HEK 293 cells were purified by protein A sepharose beads (GE Healthcare).
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bioRxiv - Cell Biology 2019Quote: ... The solubility tag was cleaved with TEV protease and the protein further purified by size exclusion chromatography (Superdex75 GL300/10/300; GE Healthcare) at 4°C in a SEC buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted proteins were digested with PreScission protease to remove tags and further purified by superose-6 increase column (GE Healthcare) in TBS buffer with 0.1% digitonin or 40 μM GDN ...
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bioRxiv - Biochemistry 2020Quote: ... All proteins were isolated from the medium exploiting the affinity of the 6xHis tag for the HisTrap Excel (GE Healthcare, USA) affinity column ...
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bioRxiv - Developmental Biology 2019Quote: Full-length dP75 protein with GST tag was purified from BL21 E.coli by using Glutathione Sepharose 4B GST-tagged protein purification resin (GE healthcare, 17075601) and following the protocol in GE healthcare webpage ...
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bioRxiv - Molecular Biology 2021Quote: ... and expression induced with 0.5 mM IPTG for 18 h at 18°C and purified as N-terminal His6-tag or N-terminal His6-GST tag fusion proteins by affinity chromatography and size exclusion chromatography using a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated in 50 mM Tris/HCl ...
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bioRxiv - Biophysics 2020Quote: ... was separated from the remaining tag protein and protease by immobilized metal affinity chromatography (IMAC) using Ni Sepharose 6 Fast flow resin (GE Healthcare). The unbound tag-free protein was collected and protein concentration was determined by absorbance (A280 ...
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bioRxiv - Biochemistry 2022Quote: ... TEV and residual affinity tag was removed by passing the protein through a Superdex 200 Increase 10/300 GL (GE Healthcare) in SEC buffer (30 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2019Quote: ... with expression induced with 0.5 mM IPTG for 18 h at 18°C and purified as N-terminal His6-tag or N-terminal His6-GST tag fusion proteins by affinity chromatography and size exclusion chromatography using a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated in 50 mM Tris/HCl ...
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bioRxiv - Molecular Biology 2019Quote: ... was added to the protein solution to a final concentration of 1.3 μM and incubated for 15 minutes at room temperature in order to cleave off His10-SUMO tag and the protein solution was applied on HisTrap 1 HP (GE Healthcare) loaded with Zn2+ and pre-equilibrated with storage buffer ...
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bioRxiv - Immunology 2019Quote: ... ERV-K envelope protein was cloned out of the sequencing vector and ligated into pGEX-6p-1 N’ GST-tag expression vector (GE Healthcare) between EcoRI and NotI sites using the following primers:
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bioRxiv - Cell Biology 2019Quote: ... was expressed as fusion protein with an N-terminal glutathione-S-transferase (GST)-tag using the pGEX-6P-1 vector (GE Healthcare). A synthetic PNPLA1 gene ...
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bioRxiv - Immunology 2021Quote: ... The N and RBD proteins contained a histidine tag and were purified through affinity chromatography step with the Histrap HP (GE HealthCare) column following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... Uncleaved protein and cleaved hexahistidine tag were removed from the eluted mixture with a HisTrap HP 5 mL column (GE Healthcare) in PBS with 25 mM imidazole ...
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bioRxiv - Plant Biology 2022Quote: ... Separation of cleavage tags and aggregated proteins was performed using size-exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in 20□mM citric acid pH 5.0 ...
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bioRxiv - Microbiology 2023Quote: ... the negatively charged N-terminal His- and BAP-tag fused MBP protein was separated from HphA by ion-exchange chromatography with HiTrap Q HP 5ml column (GE Healthcare). The flow-through ...
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bioRxiv - Biochemistry 2023Quote: ... 4°C) and the soluble proteins with His6 tag were purified on 1ml of nickel phosphate loaded sepharose beads (GE Healthcare). Bound proteins were washed in lysis buffer with 25mM imidazole then with 400mM Imidazole ...
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bioRxiv - Cell Biology 2022Quote: ... The tag-free protein was further purified by size-exclusion chromatography on a Hiload 16/60 Superdex 75 column (GE Healthcare). Mutants and ZF truncations were expressed and purified using the same procedure as for the wild-type proteins ...
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bioRxiv - Plant Biology 2022Quote: ... 4°C for 30min and used for the purification of HIS-AgLTP24 using His GravityTrap columns (GE Healthcare) with modified binding (100mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... Escherichia coli BL21 (GE Healthcare) was transformed with each GST-fusion protein construct and the expression of GST-fusion protein was induced by 0.1 mM isopropyl-β-D-thiogalactoside (IPTG ...
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bioRxiv - Cell Biology 2020Quote: ... anti-His (1:5000, mouse; GE Healthcare) and anti-GST (1:5000 ...
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bioRxiv - Microbiology 2022Quote: ... using a His SpinTrap column (GE Healthcare). Resultant elution fractions were subjected to desalting and concentration using an Amicon Ultra centrifugal filter unit (30 kDa cutoff ...
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bioRxiv - Microbiology 2023Quote: ... Anti-His antibody was from GE Healthcare; anti-PhoP antibody was elicited in rabbit (AlphaOmegaSciences) ...
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bioRxiv - Biophysics 2023Quote: ... followed by Hi-Trap Heparin (GE Healthcare) and Mono Q anion exchange chromatography columns (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... His GraviTrapTM gravity flow columns (GE Healthcare) were used for protein purification ...
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bioRxiv - Cell Biology 2024Quote: ... His Mag Sepharose Ni beads (GE Healthcare) were used for the protein-protein interaction assay ...
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bioRxiv - Cell Biology 2019Quote: ... and 0.5 mM PMSF. Mouse anti-Spt6 antibody (25C3; E. Kremmer/A. Schepers) was coupled to Protein A-Sepharose CL-4B (GE Healthcare) beads and stored at 4°C until actual pulldown ...
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bioRxiv - Genomics 2024Quote: ... WSC3-e cells (1.6 µg protein µL-1) was loaded onto a Superdex 200 10/300 column (GE Healthcare, Chicago, USA), which was equilibrated with a buffer containing 50 mM Tris-HCl (pH 7.84) ...
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bioRxiv - Biochemistry 2023Quote: ... Transfected cells were harvested 72 hours post-transfection and the 6 × His-tagged protein in the supernatant was purified using Ni-NTA (GE Healthcare, USA) affinity chromatography ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein fraction obtained was further subjected to Hi-Load Superdex 200 pg 16/600 size-exclusion chromatography column (GE Healthcare/Cytiva) equilibrated with buffer B (20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: The supernatant fraction containing the rhGFAT2 protein (with or without tag) was subjected to purification using a Ni+2NTA affinity column (HisTrap HP 5 mL, GE Healthcare, USA). The column was equilibrated with 10 column volumes (CV ...
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bioRxiv - Cell Biology 2020Quote: ... and HA-catalase were expressed as GST fusion proteins in Escherichia coli DH5α and were purified with glutathione-Sepharose beads (GE Healthcare), as described (Otera et al. ...
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bioRxiv - Cell Biology 2020Quote: ... was purified using an ÄKTAprime plus fast protein liquid chromatography (FPLC) system equipped with a 1-mL His-Trap HP column (GE Healthcare-Amersham Biosciences) and the high salt buffer ...
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bioRxiv - Microbiology 2019Quote: ... YpdAC14A and Tpx-roGFP2 proteins were purified using His Trap™ HP Ni-NTA columns (5 ml; GE Healthcare, Chalfont St Giles, UK) and the ÄKTA purifier liquid chromatography system (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was filtered using 0.45 micrometer membranes (PES, ClearLine) and the protein purification was carried out by IMAC using 1 mL His-Trap FF columns (GE Healthcare Life Sciences) and a NGC chromatography system (Bio-Rad) ...
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bioRxiv - Immunology 2019Quote: ... recombinant proteins secreted by transfected Leishmania cells were purified by liquid chromatography over His-Trap™ excel columns (GE Healthcare, Buckinghamshire, United Kingdom). Protein-containing fractions were pooled and dialysed two times against PBS (4 h and overnight) ...
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bioRxiv - Cell Biology 2020Quote: ... e Image-Quant LAS 4000 (GE Healthcare). Densitometry analysis of the gels was performed using the Image J software (version 1.37) ...
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bioRxiv - Biochemistry 2023Quote: His-purified CsoSCA-MBP-his was run on a Superose 6 Increase 10/300 GL size exclusion column (GE Healthcare) to remove MBP-his contamination ...
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bioRxiv - Microbiology 2019Quote: ... The bacterial protein PA1217 was fused to a GST-tag by cloning it into a pGEX-6P-1 vector (GE Healthcare, Chicago, IL) using the BamHI and EcoRI restriction sites (Thermo Scientific) ...
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bioRxiv - Immunology 2020Quote: ... and the C-terminal recombinant proteins containing a N-terminal 6xHis-tag were purified from the resulting supernatant using Hitrap Ni-NTA column (GE Healthcare, Uppsala, Sweden) according to the manufacturer instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... coli knockout collection (Dharmacon, GE Healthcare). Hedamycin was obtained from the National Cancer Institute’s Developmental Therapeutic Program (NCI DTP ...
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bioRxiv - Plant Biology 2023Quote: ... coli vector pGEX-2TK (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2019Quote: ... and anti-His (GE Healthcare, Chicago, IL, USA) primary antibodies ...
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bioRxiv - Immunology 2021Quote: ... A Hi-Trap® desalting column (GE Healthcare), equilibrated with argon-flushed 100 mM sodium phosphate pH 7.4 buffer ...
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bioRxiv - Immunology 2021Quote: ... A Hi-Trap® desalting column (GE Healthcare), equilibrated with 20 mM sodium phosphate buffer pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... and purified using His-Trap HP (GE Healthcare) followed by gel filtration using HiLoad 16/60 Superdex 75 columns on an ÄKTA Xpress ...
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bioRxiv - Biophysics 2024Quote: ... loaded onto a His-Trap column (GE Healthcare), and eluted by applying a linear imidazole gradient from 20 mM to 200 mM over 30 column volumes ...