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Citations for GE Life Sciences :
451 - 500 of 2832 citations for 8 Chloro 4 methyl 1 2 dihydroquinolin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Full-length nsp1 was synthesized as a gene fragment from Integrated DNA technologies (IDT) and cloned into the EcoR1 restriction site of pGEX-6P-2 (GE healthcare) using in-fusion cloning (TakaraBio) ...
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bioRxiv - Immunology 2020Quote: ... An equal volume of buffer (2 mM EDTA in PBS) and blood was mixed and carefully layered over 5ml Ficoll Hypaque solution (GE Healthcare). After centrifugation for 20 min at 900 g (with no break ...
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bioRxiv - Immunology 2021Quote: The binding affinities of antibodies to SARS-CoV-2 spike protein were determined using surface plasmon resonance (SPR) and a BIAcore T200 instrument (GE Healthcare) at 25°C ...
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bioRxiv - Immunology 2021Quote: ... and ACE2(HH:NN)-Fc constructs were captured on flow cell 2 of a Series S Protein A sensor chip (GE Healthcare – 29127555) to a density of 500 RU using a Biacore 8K instrument (GE Healthcare) ...
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bioRxiv - Bioengineering 2021Quote: ... Sonications (10-ms bursts applied at 2 Hz for 100 s) were applied coincident with an injection of the MB ultrasound contrast agent Optison (GE Healthcare, Little Chalfont ...
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bioRxiv - Cell Biology 2020Quote: ... and differentiation was induced in differentiation medium (DM) for hMB consisting of DMEM (Nacalai) with 2% horse serum (HS) (HyClone; GE Healthcare) and PS.
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bioRxiv - Cancer Biology 2022Quote: ... were resuspended in 500 μl IP buffer and incubated with the appropriate antibodies on ice for 2 h and then 30 μl protein-G-sepharose beads (GE Healthcare; equilibrated once in 10 ml water and three times in washing buffer ...
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bioRxiv - Biochemistry 2022Quote: ... secondary antibodies were incubated for 2 h at room temperature and visualized on X-ray films using enhanced chemiluminescence detection kit (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... The remaining lysate supernatant was incubated for 2 h on a spinning wheel with 200 µl of high-Performance strepTactin Sepharose beads (#28-9355-99, GE healthcare). The beads were collected by centrifugation at 1600 g at 4°C for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein samples (100 μl at 2 mg/ml) were resolved using a Superdex 200 10/300 analytical gel filtration column (GE Healthcare) running at 0.5 ml/min at 25 °C in 10 mM K-HEPES pH 8.0 ...
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bioRxiv - Immunology 2022Quote: ... The ACE2 receptor or SARS-CoV-2 spike-specific antibodies (CR3022 or S309) were immobilized on the protein A sensor chip (GE Healthcare) at a ligand capture level of ∼100 RU ...
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bioRxiv - Biochemistry 2023Quote: ... and 2 mM DTT) for 16 h before further purification by anion exchange chromatography on a HiTrap Q HP column (GE Healthcare), followed by size exclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2023Quote: ... On day 2, cells were changed with fresh media containing RMPI1640 (Thermo, 21870) supplemented with 0.2% Hyclone FBS (GE Healthcare, SH30070.03) 100 ng/mL Activin A (R&D Systems ...
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bioRxiv - Microbiology 2023Quote: ... F045 and TriHSB.2 constructs contain C-terminal 6xHis tags and were purified by passage over a HisTrapFF column (GE Healthcare), followed by size exclusion chromatography using a Superdex 200 increase 10/300 column (Cytiva) ...
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bioRxiv - Cell Biology 2023Quote: ... Finally membranes were again washed three times with TBST and submerged in SuperSignal West Pico chemiluminescent substrate for 2 min before visualizing through ImageQuant LAS 4000 chemiluminescent Image analyzer (GE Healthcare). We used a variety of inhibitors including Torin 1 (inh-tor1 ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were then incubated for 2-h at room temperature with rabbit HRP-linked IgG (GE Healthcare, Chicago, IL, USA), and a chemiluminescence detection reagent (SuperSignal™ West Pico PLUS Chemiluminescent Substrate ...
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bioRxiv - Neuroscience 2023Quote: ... The PAGE gel was exposed to a phosphorimage screen for ∼2 hours and analyzed using a Typhoon imaging system (GE Healthcare). Band intensities corresponding to the uncleaved ribozymes and the two products of self-scission were analyzed using ImageQuant (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... M was concentrated to 2 ml using Vivaspin20 columns (SartoriusStedimBiotec) and purified on a HiLoad 10/600 Superdex S200 column (GE Healthcare) in 50 mM NaH2PO4-Na2HPO4 ...
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bioRxiv - Neuroscience 2022Quote: ... The blots were visualised with the BioRad ChemiDoc XRS+ (Cohort 2) or the Amersham 6000 Gel Imager (GE Healthcare, Cohort 3), and quantified with Image Lab Software (BioRad) ...
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bioRxiv - Biophysics 2022Quote: ... 250 μL protein at a concentration of 2-3 mg/mL was injected on a Superdex200 10/300 increase column (GE Healthcare) equilibrated with 20 mM Tris pH 7 ...
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bioRxiv - Biophysics 2022Quote: ... TCA extracts of total yeast or 2 μg of mitochondrial proteins were loaded on a 12 % SDS-PAGE and transferred onto a nitrocellulose membrane (GE Healthcare). UCP1 was detected using a mouse anti-pentahistidine tag:HRP 0.2 μL/mL (MCA5995P – Bio-RAD) ...
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bioRxiv - Genetics 2023Quote: ... (2) 75 µl blood to prepare Dried Blood Spots (DBS, Whatman® 903 generic multipart filter paper, GE Healthcare, Westborough, USA) for metabolic profiling ...
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bioRxiv - Neuroscience 2023Quote: ... 100 µl of 100% ethanol or 10% DEET in ethanol was applied to a 2 cm x 6 cm strip of Whatman filter paper (GE Healthcare). The treated paper was hung from a rectangular plastic frame in front of the Peltier element ...
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bioRxiv - Immunology 2023Quote: The binding kinetics and affinity of monoclonal antibodies to the SARS-CoV-2 spike trimer were analyzed by SPR (Biacore T100, GE Healthcare). Specifically ...
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bioRxiv - Biophysics 2023Quote: ... The sample was then diluted with dilution buffer (50 mM HEPES (pH 7.2) and 5% glycerol) to reduce the salt concentration and loaded onto a Mono S 10/100 GL column (GE Healthcare). The column was washed with buffer C (50 mM HEPES (pH 7.2) ...
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bioRxiv - Immunology 2024Quote: IgGs were purified from ∼2 mL of plasma with an equal volume of Protein A Sepharose resin (GE Healthcare Life Sciences) and incubated with the resin for three days at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Concentrated FhES (10 mg total protein) was fractionated (2 mL/fraction) by SEC on a Sephacryl S-200 HR 26/60 column (GE Healthcare) connected to an AKTA Start automated liquid chromatography system (GE Healthcare) ...
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bioRxiv - Biophysics 2024Quote: ... Samples were run for 2 h at 90 V and then scanned using a Typhoon FLA 9500 laser scanner (GE Healthcare) at a pixel size of 50 µm ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated with blocking solution (2.5 % FBS, 200 mM glycine, 0.1 % Triton X-100 in PBS or 2 % bovine serum albumin (GE Healthcare or Calbiochem), 0.01 % Tween 20 (National Diagnostics or Roth ...
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bioRxiv - Cell Biology 2019Quote: ... Selected plakoglobin-containing fractions (Fig. 1d, fractions #8-11) were combined and loaded onto a 1 ml Resource Q anion exchange column (GE Healthcare Life Sciences, UK), equilibrated with 20 mM Tris pH 7.2 and 500 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... The IgG anti-SARS-CoV-2 was purified from plasma of a convalescent patient using a 5/5 HiTrap rProtein-A column (GE Healthcare, USA). See detailed information about this procedure in Supplementary method No.1.
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bioRxiv - Biochemistry 2021Quote: ... The eluted protein was cleaved with Precission™ protease for 2 hours at room temperature and applied to a 5 mL HiTrap Q HP column (GE Healthcare). Protein was eluted from the Q HP column with a 2M NaCl buffer in a 100 mL gradient ...
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bioRxiv - Genetics 2021Quote: SARS-CoV-2 receptor binding domain binding to human extracellular ACE2 were analysed on a Biacore T200 instrument (GE Healthcare Life Sciences) at 37°C and a flow rate of 30 μl/min ...
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bioRxiv - Plant Biology 2020Quote: ... coli codon optimized bZip domain of protein Cre06.g310500.t1.2 (amino acid position 141-203) was cloned into the GST-containing vector pGEX-4T-2 (GE Healthcare Life Sciences) by the company GeneScript ...
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bioRxiv - Biochemistry 2021Quote: ... The buffer exchange of fragments produced by limited proteolysis (2 mg/mL) was performed using SEC on a Superdex 200 Increase 3.2/300 column (GE Healthcare Life Science) with 100 mM NH4CH3CO2 at pH 6.8 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 49A were generated by inserting the BamHI–EcoRI PCR fragments amplified from pEGFP plasmids into the BamHI–EcoRI sites of the N-terminal GST-fused protein expression vector pGEX-6P-2 (GE Healthcare, US). Recombinant proteins were obtained as described in Supporting Methods 1 ...
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bioRxiv - Biophysics 2020Quote: ... The harvested supernatant was diluted with buffer A to a final DDM concentration of 2 mM and loaded to Ni2+-Sepharose™ 6 fast flow resin (GE Healthcare, already equilibrated with 10 CV buffer A with 780 μM DDM ...
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bioRxiv - Biochemistry 2019Quote: ... 100 μL of purified protein/complex at 2-5 mg/mL was injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in a buffer containing 20 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Genomics 2019Quote: ... and whole genome amplification was achieved by multiple displacement amplification with Phi29 DNA polymerase and the Illustra GenomiPhi v.2 kit (GE Healthcare, 25660032). Amplified genomic DNA (70 ng ...
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bioRxiv - Physiology 2019Quote: ... 20 islets per well (n = 3 biological replicates per group) were transfected with control (non-targeting #2) siRNA or mouse Brd4 SMARTpool siRNA (Dharmacon GE Life Sciences) at 100 nM each with lipofectamine 3000 according to the product instructions ...
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bioRxiv - Immunology 2020Quote: SEC-purified SARS-CoV-2 RBD-SD1 was immobilized to a Ni-NTA sensor chip in a Biacore X100 (GE Life Sciences) to a response level of ~500 RUs ...
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bioRxiv - Synthetic Biology 2021Quote: ... that had been pre-equilibrated with 1.2 column volumes of sizing buffer (25 mM Tris, pH 7.5, 500 mM NaCl, 2 mM DTT, 5% glycerol) using an ATKA FPLC (GE Healthcare, Piscataway, NJ). Protein was eluted isocratically in sizing buffer over 1.3 column volumes at a flow rate of 2 ml/min collecting 3 mL fractions ...
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bioRxiv - Synthetic Biology 2021Quote: ... that had been pre-equilibrated with 1.2 column volumes of sizing buffer (25 mM Tris, pH 7.5, 250 mM NaCl, 2 mM DTT, 5% glycerol) using an ATKA Purifier (GE Healthcare, Piscataway, NJ). Protein was eluted isocratically in sizing buffer over 1.3 column volumes at a flow rate of 2 ml/min collecting 3-ml fractions ...
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bioRxiv - Molecular Biology 2021Quote: ... and analyzed (normalization, spot matching, expression analyses, and statistics) using Image Master 2-D Platinum 6 image analysis software (GE Healthcare, USA). Spots on the gel were identified and each spot was assigned a spot quantity (q) ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μL purified proteins at 2-5 mg/mL were injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in gel filtration buffer ...
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bioRxiv - Bioengineering 2020Quote: ... Angiography was also performed with a bolus intravenous injection of 2 ml/kg iohexol (Omnipaque™, 300 mg I/ml; GE Healthcare) using a CT-scanner (CT ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 2×106 CAFs were transfected with 3 nM of non-targeting control (D-001810-10-05, GE Healthcare Dharmacon, Inc., Lafayette, CO) or THY1 human siRNA (L-015337-00-0005 ...
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bioRxiv - Molecular Biology 2023Quote: ... 40 W for 2–2.5 h) followed by detection on an Amersham Typhoon Gel and Blot Imaging System (GE Healthcare Life Sciences).
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bioRxiv - Synthetic Biology 2024Quote: ... the pellet was resuspended in 18 mL of MCB1 with 0.1% BSA and 2-4.5 mL of material was layered onto stepwise Percoll (GE Healthcare, Chicago, IL, USA) MCB1 gradients with 0.1% BSA ...
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bioRxiv - Cell Biology 2024Quote: Human HeLa (ATCC® CCL-2™) were cultured in DMEM (PanEco) supplemented with 10% fetal bovine serum (FBS; HyClone/GE Healthcare) and penicillin/streptomycin ...