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Citations for GE Life Sciences :
151 - 200 of 642 citations for 8 CHLOROQUINOLIN 2 AMINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Proteins were dialyzed against 10 mM Tris (pH 8) and purified by ion exchange (Q column) followed by size exclusion (S200, GE Healthcare) chromatography ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM L-Glutamine (GE Life Sciences #SH30852.01), 100 units/mL Penicillin and 100 μg/mL Streptomycin (Corning #30-001-CI ...
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bioRxiv - Cell Biology 2021Quote: ... GST-hEBP50 in pGEX-6P-2 (GE Healthcare): GST-hEBP50/WT (AA95-168) ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM βME using Glutathione Sepharose (GE Healthcare). Protein was cleaved overnight using thrombin (Recothrom ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM βME using glutathione sepharose (GE Healthcare). Protein was cleaved overnight using thrombin (Recothrom ...
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bioRxiv - Cancer Biology 2022Quote: ... siRNA non-targeting pool #2 (GE Healthcare Dharmacon) was used as a control for all the knockdown experiments ...
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bioRxiv - Neuroscience 2020Quote: ... the 2-D Clean-Up Kit (GE Healthcare) was used following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... (2) Ni Sepharose fast flow resin (GE Healthcare) chromatography using a step elution from 50 mM to 200 mM Imidazol in Tris buffer B (20 mM Tris/HCl ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mM βME using glutathione sepharose (GE Healthcare). Protein was cleaved overnight using thrombin (Recothrom ...
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bioRxiv - Microbiology 2023Quote: ... 2 µl of either anti-GST (GE Healthcare), anti-HA (Sigma H6908) ...
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bioRxiv - Cell Biology 2023Quote: ... 2% horse serum (GE Healthcare, Vélizy-Villacoublay, France), 0.5% penicillin-streptomycin (Invitrogen)) ...
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bioRxiv - Neuroscience 2021Quote: ... Solubilized supernatant was isolated by ultracentrifugation and the supernatant was incubated for 2 h with 2 mL CNBR-activated Sepharose beads (GE healthcare) coupled with 4 mg high-affinity GFP nanobodies (2) ...
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bioRxiv - Plant Biology 2020Quote: ... the membranes were incubated for 2 h in Can Get Signal Solution 2 (Toyobo) with anti-rabbit or mouse IgG conjugated to horseradish peroxidase (GE Healthcare). After washing twice with TBST ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 S-2P and SARS-CoV-2 HexaPro S were purified over a Superose6 Increase column (GE Life Sciences).
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bioRxiv - Molecular Biology 2021Quote: ... or 8 % denaturing polyacrylamide gel (PAGE) and then transferred to a nylon membrane (Biodyne® A, PALL or Hybond-N+, GE Healthcare). After transfer ...
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bioRxiv - Immunology 2021Quote: ... Reduced proteins were run on 8%,10% or 12% SDS-PAGE gels and transferred on to 0.22 μm nitrocellulose membranes (GE Healthcare Life Sciences). Membranes were blocked using 5% skim milk in 1X Tris-buffered saline and 0.1% Tween 20 (1XTBST ...
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bioRxiv - Biochemistry 2019Quote: ... 0.0027M KCl pH 8) and they were bound to a GST affinity column (GSTrap™ Fast Flow, GE Healthcare: 17-5131-02). The column was washed (0.02M NaPO4 ...
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bioRxiv - Neuroscience 2019Quote: ... Supernatant was neutralized by addition of 1:10 vol:vol 1M Tris pH 8 and applied to a 5 mL protein A Hitrap FF column (GE Healthcare Life Sciences) equilibrated in buffer A (100 mM Tris ...
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bioRxiv - Cancer Biology 2020Quote: Protein expression on immunoblots was quantified on 8-bit gray-scale-transformed .tiff images of either scanned Amersham Hyperfilm MP (GE Healthcare, 28906838) or .tiff images obtained by the Bio-Rad ChemiDoc imaging system ...
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bioRxiv - Molecular Biology 2019Quote: ... Equal amounts of protein (10-50 µg) from the total extract were separated on 8-10% SDS-polyacrylamide gels and transferred onto nitrocellulose blotting membranes (GE Healthcare, Germany) at 12 V for 20 min in a semi-dry Trans-Blot Turbo system (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of protein samples were separated on 8% Tris/glycine gels and then transferred to nitrocellulose membranes (GE Healthcare, Uppsala, Sweden). Subsequently the membranes were incubated with primary antibodies and HRP-coupled secondary antibodies (Jackson ImmunoResearch ...
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bioRxiv - Biochemistry 2023Quote: ... Protein samples at 8-13 mg/ml were loaded onto a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM Hepes pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein samples at concentrations >8 mg/ml were loaded onto a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM HEPES pH 7.5 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and then inserted immediately upstream of a promoter-less chloramphenicol resistance gene (cat) gene in pBR322 origin a medium-copy-number plasmid pKK232-8 (GE Healthcare, USA) to create the pHL1141 plasmid (8 ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 mM imidazole at pH 8) and loaded on a nickel affinity chromatography column (1 mL HisTrap, GE Healthcare, 17-5247-01). Bound proteins were eluted in elution buffer (20 mM NaH2PO4 ...
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bioRxiv - Systems Biology 2021Quote: ... and imaged with an IN Cell Analyzer 6000 (nine fields of view per well, 20x/0.45NA air objective, 2൸2 binning) (GE Healthcare Life Sciences). Following image acquisition ...
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bioRxiv - Neuroscience 2021Quote: ... or anti-rabbit-IgG-F(ab’)2 (GE Healthcare) antibody for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... or anti-rabbit-IgG-F(ab’)2 (GE Healthcare) antibody for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... 2 ml of Chelating Sepharose Fast Flow (GE Healthcare) was added to a small plastic column and prepared for affinity purification by sequential washing with 1 column volume (CV ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2 mM DTT using PD10 columns (GE Life Sciences) and concentrated to 9 mg/mL ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM DTT by HiTrap desalting column (GE Healthcare).
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bioRxiv - Developmental Biology 2019Quote: ... 30 μg of proteins from lysates were subjected to migration on 8–12 % acrylamide gels and transferred on to polyvinylidene difluoride membranes (GE Healthcare Europe GmbH) in borate buffer (50 mM Tris-HCl and 50 mM borate ...
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bioRxiv - Neuroscience 2019Quote: Whole-brain T1-weighted MRI data were acquired using a General Electric HDx 3T MRI scanner and a 8-channel receiver head coil (GE Healthcare, Waukesha, WI) at the Cardiff University Brain Research Imaging Centre with an axial 3D fast spoiled gradient recalled sequence (echo time 3 ms ...
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bioRxiv - Biophysics 2021Quote: ... Cleaved products were further purified by S-75 size exclusion chromatography (SEC buffer-50 mM sodium phosphate, 50 mM sodium chloride, 1 mM DTT, pH 8) (GE Healthcare Life Sciences). The purity of the protein was inspected using a 15% SDS PAGE (Figure S1) ...
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bioRxiv - Immunology 2022Quote: ... Aliquots of the purified A48 or D48 PTX3 proteins were run under denaturing conditions on Tris-Acetate 3–8% (w/v) and Bis-Tris 10% (w/v) protein gels (GE Healthcare Life Sciences), in the absence and presence ...
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bioRxiv - Biochemistry 2021Quote: ... Equal amounts of protein (10–50 μg) from the total extract were separated on 8–10% SDS–polyacrylamide gels and transferred onto nitrocellulose blotting membranes (GE Healthcare, Chicago, IL) at 12 V for 20 min in a semi-dry Trans-Blot Turbo system (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... liver and enzyme treated lung were prepared by meshing organs through 70 μm nylon cell strainers in sRPMI-1640 with 8% FBS (HyClone Laboratories, GE Healthcare, Logan, Utah). After red blood cells lysis with NH4Cl (liver ...
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bioRxiv - Plant Biology 2022Quote: ... Twenty micrograms of total RNAs were separated on 15% acrylamide/8 M urea gel and transferred onto an Amersham™ Hybond™-N+ nylon membrane (GE Healthcare). The specific isotope probe was generated by end-labeling DNA oligonucleotides complementary to the miR167a sequence with [γ-32P] ATP by T4 polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Neuroscience 2023Quote: ... Then 60 μg of the total protein of each genotype was separated on 8% SDS-PAGE and transferred to the PVDF membrane (Amersham, GE Healthcare Life Sciences). The membrane was blocked in 5% skimmed milk (HIMEDIA ...
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bioRxiv - Cell Biology 2023Quote: ... High resolution images of individual PvLS parasites and PHH nuclei were obtained by capturing 8 planes in the Z dimension using a 100×objective on Deltavision Core (GE Healthcare Life Sciences) and deconvoluted using softWoRx (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2021Quote: ... 15-25 μg of total protein per sample were resolved on 8%-14% SDS-PAGE and transferred onto nitrocellulose membrane (Amersham Hybond, GE Healthcare Life Science, 10600002). Membranes were blocked in 5% milk in PBS followed by incubation with specific primary and secondary antibodies ...
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bioRxiv - Evolutionary Biology 2019Quote: ... ligation mix was diluted to 50µl and used 0.583:1 volume of home-brewed SPRI beads (SPRI binding buffer: 2.5M NaCL, 20mM PEG 8000, 10mM Tric-HCL, 1mM EDTA, oh=8, 1mL TE-washed SpeedMag beads GE Healthcare, 65152105050250 per 100mL buffer) for purification ...
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bioRxiv - Microbiology 2022Quote: ... were collected by vacuum filtration onto glass microfiber filters (grade GF/C, 47 mm in diameter, pre-combusted at 460°C for >8 h, GE Healthcare Whatman, Buckinghamshire, UK), immediately plunged into liquid nitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... Selected plakoglobin-containing fractions (Fig. 1d, fractions #8-11) were combined and loaded onto a 1 ml Resource Q anion exchange column (GE Healthcare Life Sciences, UK), equilibrated with 20 mM Tris pH 7.2 and 500 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant desmin was purified under denaturing conditions (8 M urea) by ion exchange chromatography (IEC) using 5 mL HiTrap DEAE-FF columns (GE Healthcare, Chicago, IL, USA) and in a second step by immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Cell Biology 2021Quote: ... For co-immunoprecipitation of endogenous SQSTM1/p62 the cell lysates were incubated for 2-4 h with specific antibody followed by 1-2 h with protein-G coupled Sepharose beads (GE Healthcare, 17-0885-01). To resolve protein complexes for denaturing immunoprecipitation ...
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bioRxiv - Biophysics 2019Quote: ... A drop of 5 μL of 2% dimethyldichlorosilane (GE Healthcare) was added on the mask and hard vacuum was applied for 30 min ...
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bioRxiv - Plant Biology 2020Quote: ... Flow cell (FC) 2 of an NTA chip (GE Healthcare) was activated with 30 μl 0.5 mM NiCl2 ...
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bioRxiv - Genetics 2022Quote: ... 2 ul of Streptavidin Sepharose High Performance beads (GE Healthcare) and 35 ul of H2O ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... containing 2% of Protease Inhibitor Mix (GE Healthcare, Uppsala, Sweden). We purified all samples using a 2-D Clean-Up Kit (GE Healthcare ...