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Citations for GE Life Sciences :
501 - 550 of 3020 citations for 7 bromo 1 2 3 4 tetrahydroisoquinoline 1 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... three segments of MASTL (N: aa 1-340; M: aa 335-660; C: aa 656-887) were inserted into pGEX 4T-1(GE Healthcare). The resulting expression vectors were transfected into BL21 bacteria cells for protein expression and purification ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was diluted 1:1 with solubilization buffer without detergent and lysates were pre-cleared by incubation with Protein G sepharose beads (GE Healthcare) on a rotation wheel for 2 h ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were washed three times with TBS-Tween 1 % and incubated for 1 h at room temperature with a secondary antibody anti-Mouse conjugated to horseradish peroxidase (GE Healthcare, NA9310 ...
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bioRxiv - Biochemistry 2023Quote: ... The purified protein was finally concentrated to 1–10 mg mL−1 using centrifugal concentrators with a cut-off at 100 kDa (Vivaspin 100, GE Healthcare), frozen in liquid nitrogen and stored at −80 °C in storage buffer containing 50 mM Mes-KOH (pH 7) ...
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bioRxiv - Microbiology 2024Quote: ... to reach 120 mM of NaCl and were further passed through a Hi-Trap Q HP 1 mL column to remove residual DNA before binding on a Hi-Trap heparin HP 1 mL column (GE Healthcare) and elution with a NaCl gradient ...
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bioRxiv - Molecular Biology 2024Quote: ... 1:3 and 3:1 (w/w) at a concentration of 1 mg/mL were separated on a Superdex 200 10/30 column (GE Healthcare) using a LC1100 HPLC System (Agilent Technologies ...
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bioRxiv - Biochemistry 2024Quote: ... 2 µL of a 1:1 mix of two types of commercially available carboxylate beads (Sera-Mag Speed beads, 45152105050250, 65152105050250, GE Healthcare), and 60 µL ethanol ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... CG42797EcoRI_F: 5’-CCGgaattcATGGAGCCACCAGCT-3’ and CG42797XhoI_R: 5’-TAGAGGTACCctcgagCTACTCAATGCCGAACGTGTTG-3’ and cloned by enzymatic digestion into a pGEX6P1(GE Healthcare).
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... CG42797EcoRI WWI_F: 5’-ccgGAATTCCCACCATTGCCGCCTG-3’ CG42797XhoI WWII_R: 5’-ccgCTCGAGTCAACGAGGATCCATGAA-3’ and cloned by enzymatic digestion into a pGEX6P1(GE Healthcare).
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... CG42797EcoRI_F: 5’-CCGgaattcATGGAGCCACCAGCT-3’ and CG42797XhoI_Nterm_R: 5’-CCGctcgagTCATTCGCTGGGCTGC-3’ and cloned by enzymatic digestion into a pGEX6P1(GE Healthcare).
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bioRxiv - Physiology 2019Quote: Cardiac dimensions and contractility were assessed by right para-sternal transthoracic echocardiography (Vivid 7; GE Healthcare, UK) in non-sedated conscious sheep gently restrained in a supine position ...
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bioRxiv - Plant Biology 2023Quote: ... Two mg of proteins in buffer were mixed with 50ul 7-Methyl-GTP-Sepharose 4B (GE Healthcare) previously equilibrated with extraction buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Glutathione-sepharose 4 beads (GE Healthcare) were added to remove the cleaved tags and non-cleaved protein for another 2 h at 4°C in a column under gravity flow ...
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bioRxiv - Neuroscience 2020Quote: ... The following secondary antibodies coupled to horseradish peroxidase (HRP) were applied for 1 h: sheep anti-mouse (#NA9310V, GE Healthcare, 1:4000), donkey anti-rabbit (#NA9340V ...
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bioRxiv - Systems Biology 2022Quote: ... was combined with 250 μg carboxylated paramagnetic beads (1:1 mix of hydrophiclic and hydrophobic Sera-Mag SpeedBead Carboxylate-Modified, GE Life Sciences) and 750 μl ethanol ...
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bioRxiv - Neuroscience 2022Quote: ... distilled water was replaced with a 1:1 dilute of non-ionic iodine Omnipaque 350 contrast agent (Iohexol, 350 mg iodine/ml; GE Healthcare AS) in normal saline via 3 ml syringe ...
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bioRxiv - Physiology 2022Quote: ... The specific proteins were detected with horseradish peroxidase (HRP) conjugated secondary antibodies (1:5000 dilution; CtNo_NA934, GE Healthcare, 1:5000 dilution; CtNo_NA931, GE Healthcare, Tokyo, Japan), developed with Immobilon Western Chemiluminescent HRP Substrate (CtNo_WBKLS0100 ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibodies were horseradish peroxidase (HPR)-conjugated (Sigma-Aldrich A0545, anti-rabbit 1:10000; GE Healthcare NA931V, anti-mouse 1:5000). Protein expression was detected after 3 min incubation with a chemoluminescent substrate (GE Healthcare RPN2106 for GAPDH ...
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bioRxiv - Biochemistry 2023Quote: The DNA sequences encoding the DNA binding domains of Pdr1p (residues 1-207) and Pdr3p (residues 1-184) were amplified by PCR from the Yeast Genomic Tiling Collection (GE Healthcare YSC4613). The PCR products were inserted into the pMCSG7 vector by ligation independent cloning ...
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bioRxiv - Cancer Biology 2023Quote: WDR5 (residues 22-334 or 1-334) and PTENα (residues 1-576) proteins were further purified using a Superdex200 gel-filtration column (GE Healthcare, 28989335) with a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Immunology 2023Quote: ... to a ratio of 1 to 1 before being layered onto Ficoll Paque (1.077 g/ml; GE Healthcare Europe GmbH, Freiburg, Germany). After centrifugation (800 x g for 25 min) ...
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bioRxiv - Microbiology 2024Quote: ... the following HRP conjugated secondary antibodies were used: for Cas9 - anti-mouse (1:10,000 in 1% milk PBS-T, GE Healthcare, code NA931V); for γ-H2A - anti-rabbit (1:2000 in 1% milk/PBS-T ...
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bioRxiv - Microbiology 2024Quote: ... 2 µMnt ssDNA (Ovio 45) and 2 mM ATP (GE Healthcare) in a 40 µL reaction solution containing 10 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genomics 2019Quote: ... The bioreactors were inoculated at a target cell density of 0.4 × 106 cells/mL in ActiPro batch media and fed daily beginning on Day 3 (3% (v/v) Boost 7a and 0.3% (v/v) Boost 7b (GE Healthcare)) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were reverse-transfected using DharmaFECT 1 (GE Healthcare) and then transfected again 24 h later according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... and sheep anti-mouse (NA931, GE Healthcare, 1:10000). The guinea-pig anti-KCTD16 antibody was raised against a synthetic peptide derived from mouse KCTD16 (aa7-23 ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mL Q Sepharose® column (GE Healthcare 17051005) was charged with 1 M KCl and then equilibrated with elution buffer containing 250 mM imidazole ...
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bioRxiv - Genomics 2020Quote: ... HRP-conjugated anti-mouse (GE Healthcare NA931V, 1:10000) and HRP-conjugated anti-rabbit (Santa Cruz sc-2004 1:5000 ...
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bioRxiv - Biochemistry 2020Quote: ... a modified version of pGEX-4T-1 (GE Healthcare) where the thrombin site is replaced with a TEV cleavage site.
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bioRxiv - Immunology 2019Quote: ... 1 μCi/well of [3H]-thymidine (GE Healthcare, UK) was added ...
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bioRxiv - Genomics 2021Quote: ... with 1 mL Histrap column (GE Healthcare Life Sciences) pre-equilibrated with buffer A ...
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bioRxiv - Plant Biology 2021Quote: ... 1% (w/v) dithiothreitol (DTT; GE Healthcare, Piscataway, USA), and 10 μL of EDTA-free Halt protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-mouse (GE Healthcare Cat# NA931, 1:5000 dilution), and then detected using an ECL SuperSignal West Femto y West Pico detection kit (Pierce).
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bioRxiv - Cell Biology 2022Quote: ... Dharmacon DharmaFECT 1 transfection reagent (GE Healthcare, Manchester, UK) was used following the manufacturer’s siRNA transfection protocol with slight modification ...
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bioRxiv - Developmental Biology 2022Quote: ... mouse IgG HRP linked (1:7,500, NXA931V, GE Healthcare). We used ECL Western Blotting detection reagent (RPN2232 and RPN2209 ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM DTT using Akta FPLC system (GE Healthcare) at 0.2 mL/min ...
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bioRxiv - Immunology 2021Quote: ... followed by anion exchange (1 ml HiTrapQ, GE Healthcare), according to manufacturers instructions ...
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bioRxiv - Neuroscience 2019Quote: ... or anti-rabbit HRP-conjugated (1:20,000; GE Healthcare) in blocking solution for 1 h at room temperature ...
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bioRxiv - Biophysics 2019Quote: ... or a 1 ml HisTrap Excel column (GE Healthcare) equilibrated in wash buffer (50 mM Tris-HCl pH 7.5 ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... 1 mM DTT) using a HiTrap column (GE Healthcare). The resulting eluate was passed over 1 mL Streptactin XT high capacity beads (IBA ...
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bioRxiv - Neuroscience 2020Quote: ... antibody with respective secondary antibodies (1:5000, GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... The pGEX-6P-1 vector (GE Healthcare, Uppsala, Sweden) was also amplified and modified by inverse PCR using primer pair For_PreSci_GST and Rev_PG_Met ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and anti-rabbit conjugated HRP (1:500; GE Healthcare) as secondary antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... Vector Map: pLKO.1) were purchased from GE Healthcare Dharmacon ...
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bioRxiv - Biochemistry 2019Quote: ... with a distal 1 ml GSTrap 4B (GE Healthcare), connected in series ...
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bioRxiv - Biochemistry 2021Quote: ... and a 1 ml HiTrap Q column (GE Healthcare), both equilibrated in a buffer containing 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2020Quote: ... and HRP-anti mouse secondary (GE Healthcare; 1:5000). Western blots were developed on X-ray film using a SRX-101A table top film processor.
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bioRxiv - Physiology 2021Quote: ... followed by biotinylated antibody (GE Healthcare, RPN1004V, 1:200), HRP-labeled streptavidin (Southern Biotech ...
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bioRxiv - Biophysics 2020Quote: ... through a 1 mL HisTrap HP column (GE Healthcare) and eluting with 350 mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... and sheep anti-mouse IgG (GE Healthcare, 1:2000) HRP-conjugated secondary antibody ...